Abstract：
:Trace metals are required in many cellular processes in bacteria but also induce toxic effects to cells when present in excess. As such, various forms of adaptive responses towards extracellular trace metal ions are essential for the survival and fitness of bacteria in their environment. A soil Pseudomonas putida, strain S13.1.2 has been isolated from French vineyard soil samples, and shown to confer resistance to copper ions. Further investigation revealed a high capacity to tolerate elevated concentrations of various heavy metals including nickel, cobalt, cadmium, zinc and arsenic. The complete genome analysis was conducted using single-molecule real-time (SMRT) sequencing and the genome consisted in a single chromosome at the size of 6.6 Mb. Presence of operons and gene clusters such as cop, cus, czc, nik, and asc systems were detected and accounted for the observed resistance phenotypes. The unique features in terms of specificity and arrangements of some genetic determinants were also highlighted in the study. Our findings has provided insights into the adaptation of this strain to accumulation and persistence of copper and other heavy metals in vineyard soil environment.
journal_name
AMB Expressjournal_title
AMB Expressauthors
Chong TM,Yin WF,Chen JW,Mondy S,Grandclément C,Faure D,Dessaux Y,Chan KGdoi
10.1186/s13568-016-0269-xsubject
Has Abstractpub_date
2016-12-01 00:00:00pages
95issue
1issn
2191-0855pii
10.1186/s13568-016-0269-xjournal_volume
6pub_type
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