The heat shock protein 70 gene as a new alternative molecular marker for the taxonomic identification of Streptomyces strains.

abstract：:The discovery and creation of biocatalysts for plant biomass conversion are essential for industrial demand and scientific research of the plant cell wall. α-1,2 and α-1,3-L-arabinofuranosidases are debranching enzymes that catalyzing hydrolytic release of α-L-arabinofuranosyl residues in plant cell wall. Gene databas...

journal_title：AMB Express

authors： Hu Y,Yan X,Zhang H,Liu J,Luo F,Cui Y,Wang W,Zhou Y

更新日期：2018-04-02 00:00:00

abstract：:The bioprospection for cellulase and protease producers is a promise strategy for the discovery of potential biocatalysts for use in hydrolysis of lignocellulosic materials as well as proteic residues. These enzymes can increment and turn viable the production of second generation ethanol from different and alternativ...

journal_title：AMB Express

authors： da Cruz Ramos GF,Ramos PL,Passarini MRZ,Vieira Silveira MA,Okamoto DN,de Oliveira LCG,Zezzo LV,Marem A,Santos Rocha RC,da Cruz JB,Juliano L,de Vasconcellos SP

更新日期：2016-03-01 00:00:00

abstract：:Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and is highly abundant in Rehmannia glutinosa Libosch. The study included five groups such as group I (normal contr...

journal_title：AMB Express

authors： Yang L,Feng X,Li Y,Zhang S,Ying Y

更新日期：2020-03-20 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L...

journal_title：AMB Express

authors： Bel-Rhlid R,Thapa D,Kraehenbuehl K,Hansen CE,Fischer L

更新日期：2013-05-21 00:00:00

abstract：: In this study manganese peroxidase (MnP) enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid media and a simplified process was established for the purification of extracellular enzymes.Five lignin degra...

journal_title：AMB Express

authors： Järvinen J,Taskila S,Isomäki R,Ojamo H

更新日期：2012-11-29 00:00:00

abstract：:Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressu...

journal_title：AMB Express

authors： Sakudo A,Yagyu Y

更新日期：2021-01-09 00:00:00

abstract：:Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosacc...

journal_title：AMB Express

authors： Zhang Y,Yang H,Yu X,Kong H,Chen J,Luo H,Bai Y,Yao B

更新日期：2019-01-29 00:00:00

abstract：:High-performance liquid chromatography-mass spectrometry (LC-MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis ...

journal_title：AMB Express

authors： Gu F,Chen Y,Hong Y,Fang Y,Tan L

更新日期：2017-12-01 00:00:00

abstract：: Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus ha...

journal_title：AMB Express

authors： Martínez CP,Echeverri C,Florez JC,Gaitan AL,Góngora CE

更新日期：2012-03-30 00:00:00

abstract：:The effects of inlet VOCs (Volatile Organic Compounds) shifts on microbial community structure in a biofiltration system were investigated. A lab-scale biofilter was set up to treat eight VOCs sequentially. Short declines in removal efficiency appeared after VOCs shifts and then later recovered. The number of OTUs in ...

journal_title：AMB Express

authors： Lu L,Wang G,Yeung M,Xi J,Hu HY

更新日期：2018-10-03 00:00:00

abstract：:Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attemp...

journal_title：AMB Express

authors： Watanabe Y,Kuroda K,Tatemichi Y,Nakahara T,Aoki W,Ueda M

更新日期：2020-04-15 00:00:00

abstract：:During industrial fermentation, Streptomyces clavuligerus F613-1 simultaneously produces primary product clavulanic acid (CA) and cephamycin C. The cephamycin C biosynthetic gene cluster and pathway have been basically elucidated and the CcaR positive regulator was found to control the cephamycin genes expression. How...

journal_title：AMB Express

authors： Fu J,Qin R,Zong G,Zhong C,Zhang P,Kang N,Qi X,Cao G

更新日期：2019-07-27 00:00:00

abstract：:Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. How...

journal_title：AMB Express

authors： Hu X,Xu N,Yang X,Hu X,Zheng Y,Zhang Q

更新日期：2020-10-04 00:00:00

abstract：:Hydrogen gas represents a promising alternative energy source to dwindling fossil fuel reserves, as it carries the highest energy per unit mass and its combustion results in the release of water vapour as only byproduct. The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hyd...

journal_title：AMB Express

authors： Mohr T,Aliyu H,Biebinger L,Gödert R,Hornberger A,Cowan D,de Maayer P,Neumann A

更新日期：2019-12-23 00:00:00

abstract：:A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs). P.putida LS46 was differentiated from other P.putida strains on the basis of cpn60 (UT). The complete genome of P.putida LS46 was sequenced and annotate...

journal_title：AMB Express

authors： Sharma PK,Fu J,Zhang X,Fristensky B,Sparling R,Levin DB

更新日期：2014-05-22 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

journal_title：AMB Express

authors： Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

更新日期：2020-12-11 00:00:00

abstract：:An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experiment...

journal_title：AMB Express

authors： Abdelrazek NA,Elkhatib WF,Raafat MM,Aboulwafa MM

更新日期：2020-04-15 00:00:00

abstract：:Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, t...

journal_title：AMB Express

authors： de Carvalho CCCR,Fischer MA,Kirsten S,Würz B,Wick LY,Heipieper HJ

更新日期：2016-12-01 00:00:00

abstract：:Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of ...

journal_title：AMB Express

authors： Gao D,Chang K,Ding G,Wu H,Chen Y,Jia M,Liu X,Wang S,Jin Y,Pan H,Li H

更新日期：2019-05-24 00:00:00

abstract：:Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in ...

journal_title：AMB Express

authors： Othman M,Ariff AB,Wasoh H,Kapri MR,Halim M

更新日期：2017-11-27 00:00:00

abstract：:Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production. The hydrolytic pathway from ginsenoside Rb1 to compound K via Rd...

journal_title：AMB Express

authors： Shin KC,Choi HY,Seo MJ,Oh DK

更新日期：2017-10-04 00:00:00

abstract：:Protein-ligand docking plays an important role in computer-aided pharmaceutical development. Protein-ligand docking can be defined as a search algorithm with a scoring function, whose aim is to determine the conformation of the ligand and the receptor with the lowest energy. Hence, to improve an efficient algorithm ha...

journal_title：AMB Express

authors： Guan B,Zhang C,Ning J

更新日期：2017-09-13 00:00:00

abstract：:Chlorothalonil, a non-systemic and broad-spectrum fungicide, is widely used to control the pathogens of agricultural plants. Although microbial degradation of chlorothalonil is known, we know little about the colonization and degradation capacity of these microbes in the natural and semi-natural soil environments. The...

journal_title：AMB Express

authors： Zhang Q,Saleem M,Wang C

更新日期：2017-12-23 00:00:00

abstract：:The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to ass...

journal_title：AMB Express

authors： Kamira B,Shi LL,Fan LM,Zhang C,Zheng Y,Song C,Meng SL,Hu GD,Bing XW,Chen ZJ,Xu P

更新日期：2018-08-28 00:00:00

abstract：:Disentangling the interactions between cyanobacteria and associated bacterial community is important for understanding the mechanisms that mediate the formation of cyanobacterial blooms in freshwater ecosystems. Despite the fact that a metagenomic approach enables researchers to profile the structure of microbial comm...

journal_title：AMB Express

authors： Ren M,Zhang G,Ye Z,Qiao Z,Xie M,Lin Y,Li T,Zhao J

更新日期：2017-11-02 00:00:00

abstract：: Effective utilization of photosynthetic microorganisms as potential biocatalysts is favorable for the production of useful biomaterials and the reduction of atmospheric CO2. For example, biocatalytic transformations are used in the synthesis of optically active alcohols. We previously found that ketone reduction in c...

journal_title：AMB Express

authors： Yamanaka R,Nakamura K,Murakami A

更新日期：2011-09-01 00:00:00

abstract：:Docosahexaenoic acid (DHA) enriched in brain can yield many important degradation products after the attack of hydroxyl radicals, which is known to serve as a nutraceutical and neuroprotective effects. Oxidative stress is a commonly observed feature of Alzheimer's disease (AD). Therefore, uniformly radiolabeled DHA pl...

journal_title：AMB Express

authors： Song P,Kuryatov A,Axelsen PH

更新日期：2020-03-11 00:00:00

abstract：:Spinal cord injury (SCI) is involved with abnormal expression of miRNAs (miRs) which are responsible for some IIry injury responses which include apoptosis, inflammation and oxidative stress. Mechanisms involving miRs induced apoptosis still needs to be investigated. In the present work we developed a rat model of SCI...

journal_title：AMB Express

authors： An Y,Li J,Yuan Q,Fan M

更新日期：2020-06-15 00:00:00

abstract：:Recombinant antibodies are used with great success in many different diagnostic and therapeutic applications. A variety of protein expression systems are available, but nowadays almost all therapeutic antibodies are produced in mammalian cell lines due to their complex structure and glycosylation requirements. However...

journal_title：AMB Express

authors： Blanco-Toribio A,Álvarez-Cienfuegos A,Sainz-Pastor N,Merino N,Compte M,Sanz L,Blanco FJ,Álvarez-Vallina L

更新日期：2015-12-01 00:00:00