NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level.

abstract：:Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of ...

journal_title：AMB Express

authors： Gao D,Chang K,Ding G,Wu H,Chen Y,Jia M,Liu X,Wang S,Jin Y,Pan H,Li H

更新日期：2019-05-24 00:00:00

abstract：:The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs functi...

journal_title：AMB Express

authors： Aghajanyan A,Gabrielyan L,Schubert R,Trchounian A

更新日期：2020-04-07 00:00:00

abstract：:High temperature causes ubiquitous environmental stress to microorganisms, but studies have not fully explained whether and to what extent heat shock would affect genome stability. Hence, this study explored heat-shock-induced genomic alterations in the yeast Saccharomyces cerevisiae. Using genetic screening systems a...

journal_title：AMB Express

authors： Shen L,Wang YT,Tang XX,Zhang K,Wang PM,Sui Y,Zheng DQ

更新日期：2020-08-17 00:00:00

abstract：:Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

journal_title：AMB Express

authors： Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

更新日期：2018-08-06 00:00:00

abstract：:Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressu...

journal_title：AMB Express

authors： Sakudo A,Yagyu Y

更新日期：2021-01-09 00:00:00

abstract：:The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

journal_title：AMB Express

authors： Shonyela SM,Feng B,Yang W,Yang G,Wang C

更新日期：2020-04-17 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the prese...

journal_title：AMB Express

authors： Lee JH,Kim YG,Gwon G,Wood TK,Lee J

更新日期：2016-12-01 00:00:00

abstract：:Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the...

journal_title：AMB Express

authors： Shen Y,Li J,Xiang J,Wang J,Yin K,Liu Q

更新日期：2019-07-27 00:00:00

abstract：:Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used protein production platforms. Typically, strong, tightly regulated promoters of genes coding for their methanol utilization (MUT) pathways are used...

journal_title：AMB Express

authors： Vogl T,Fischer JE,Hyden P,Wasmayer R,Sturmberger L,Glieder A

更新日期：2020-02-25 00:00:00

abstract：:Lactic acid bacteria are industrially important microorganisms recognized for fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Fermentation conditions such as concentration of initial glucose in the culture, concentration of lactic acid accumulated in ...

journal_title：AMB Express

authors： Othman M,Ariff AB,Wasoh H,Kapri MR,Halim M

更新日期：2017-11-27 00:00:00

abstract：:Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

journal_title：AMB Express

authors： Fikry S,Khalil N,Salama O

更新日期：2019-03-26 00:00:00

abstract：:Ammonia is an essential substance for agriculture and the chemical industry. The intracellular production of ammonia in yeast (Saccharomyces cerevisiae) by metabolic engineering is difficult because yeast strongly assimilates ammonia, and the knockout of genes enabling this assimilation is lethal. Therefore, we attemp...

journal_title：AMB Express

authors： Watanabe Y,Kuroda K,Tatemichi Y,Nakahara T,Aoki W,Ueda M

更新日期：2020-04-15 00:00:00

abstract：: To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-bu...

journal_title：AMB Express

authors： Koitabashi M,Noguchi MT,Sameshima-Yamashita Y,Hiradate S,Suzuki K,Yoshida S,Watanabe T,Shinozaki Y,Tsushima S,Kitamoto HK

更新日期：2012-08-02 00:00:00

abstract：:The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial ...

journal_title：AMB Express

authors： Kaur N,Arora DS

更新日期：2020-11-11 00:00:00

abstract：:In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, ...

journal_title：AMB Express

authors： Soundhararajan R,Mohamed Sheik Meeran SAF,Prakash SP,Mohammad W,Kandasamy R,Srinivasan H

更新日期：2020-09-18 00:00:00

abstract：:Biological technologies for recycling rare metals, which are essential for high-tech products, have attracted much attention because they could prove to be more environmentally friendly and energy-saving than other methods. We have developed biological recycling technologies by cell surface engineering for the selecti...

journal_title：AMB Express

authors： Ito R,Kuroda K,Hashimoto H,Ueda M

更新日期：2016-12-01 00:00:00

abstract：:Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides. However, the majority of the studies are focussed on the marine ...

journal_title：AMB Express

authors： Gaikwad S,Shouche YS,Gade WN

更新日期：2016-12-01 00:00:00

abstract：:In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

journal_title：AMB Express

authors： Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

更新日期：2017-12-01 00:00:00

abstract：:Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli ar...

journal_title：AMB Express

authors： Mozejko-Ciesielska J,Dabrowska D,Szalewska-Palasz A,Ciesielski S

更新日期：2017-12-01 00:00:00

abstract：:Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liqui...

journal_title：AMB Express

authors： Huang L,Sun N,Ban L,Wang Y,Yang H

更新日期：2019-01-07 00:00:00

abstract：:The engineered monomeric version of the lancelet Branchiostoma lanceolatum fluorescent protein, mNeonGreen (mNG), has several positive characteristics, such as a very bright fluorescence, high photostability and fast maturation. These features make it a good candidate for the utilization as fluorescent tool for cell b...

journal_title：AMB Express

authors： Werner A,Otte KL,Stahlhut G,Pöggeler S

更新日期：2020-12-21 00:00:00

abstract：:Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

journal_title：AMB Express

authors： Ng TW,Chan WL,Lai KM

更新日期：2017-12-01 00:00:00

abstract：:Wheat bran is an effective raw material for preparation xylooligosaccharides; however, current research mainly focuses on alkali extraction and enzymatic hydrolysis methods. Since ester bonds are destroyed during the alkali extraction process, xylanase and arabinofuranosidase are mainly used to hydrolyze xylooligosacc...

journal_title：AMB Express

authors： Zhang Y,Yang H,Yu X,Kong H,Chen J,Luo H,Bai Y,Yao B

更新日期：2019-01-29 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:With the developments in taxonomy, the classically used highly conserved 16S rRNA molecular marker has shown some disadvantages among closely related species. For further taxonomic studies of the prokaryotes, specific PCR primers were designed from two conserved regions in the amino acid sequences of the 70-kDa heat s...

journal_title：AMB Express

authors： Hu Y,Sun F,Liu W

更新日期：2018-09-10 00:00:00

abstract：:Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have...

journal_title：AMB Express

authors： Almaraz-Delgado AL,Flores-Uribe J,Pérez-España VH,Salgado-Manjarrez E,Badillo-Corona JA

更新日期：2014-08-13 00:00:00

abstract：:High-performance liquid chromatography-mass spectrometry (LC-MS) was used for comprehensive metabolomic fingerprinting of vanilla fruits prepared from the curing process. In this study, the metabolic changes of vanilla pods and vanilla beans were characterized using MS-based metabolomics to elucidate the biosynthesis ...

journal_title：AMB Express

authors： Gu F,Chen Y,Hong Y,Fang Y,Tan L

更新日期：2017-12-01 00:00:00

abstract：:Plant extracts serve as reducing and coating agents and are, therefore, commonly employed for the generation of silver (Ag) nanoparticles (NPs). Plant extract mediated synthesis of Ag NPs is a green, environmentally friendly and cost-effective technique which offers a new and potential alternative to chemically synthe...

journal_title：AMB Express

authors： Hambardzumyan S,Sahakyan N,Petrosyan M,Nasim MJ,Jacob C,Trchounian A

更新日期：2020-09-05 00:00:00

abstract：:The Editor-in-Chief has retracted this article (Wang et al. 2018) because the authors do not have ownership of the data they report. An investigation by the Commonwealth Scientific and Industrial Research Organisation (CSIRO) has concluded that the data reported in this article are the sole property of the CSIRO. Ming...

journal_title：AMB Express

authors： Wang D,Liu Y,Lv D,Hu X,Zhong Q,Zhao Y,Wu M

更新日期：2019-04-19 00:00:00

abstract：:Young barley leaf extract (YBL) contains beneficial substances such as fructans, minerals, and vitamins. The effects of YBL administration on the human colonic microbiota and its production of metabolites were evaluated using an in vitro model culture system. Fermentations were started by inoculating fecal samples fro...

journal_title：AMB Express

authors： Sasaki D,Sasaki K,Kadowaki Y,Aotsuka Y,Kondo A

更新日期：2019-11-13 00:00:00