Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase.

abstract：:In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB', gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously expressed in Escherichia coli, purified as protein possessing a His-Tag and initially characterized. FadB' is...

journal_title：AMB Express

authors： Volodina E,Steinbüchel A

更新日期：2014-08-28 00:00:00

abstract：:A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase...

journal_title：AMB Express

authors： Knudsen JD,Carlquist M,Gorwa-Grauslund M

更新日期：2014-10-30 00:00:00

abstract：:Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liqui...

journal_title：AMB Express

authors： Huang L,Sun N,Ban L,Wang Y,Yang H

更新日期：2019-01-07 00:00:00

abstract：:The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to ass...

journal_title：AMB Express

authors： Kamira B,Shi LL,Fan LM,Zhang C,Zheng Y,Song C,Meng SL,Hu GD,Bing XW,Chen ZJ,Xu P

更新日期：2018-08-28 00:00:00

abstract：:The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broa...

journal_title：AMB Express

authors： Ao XL,Yu X,Wu DT,Li C,Zhang T,Liu SL,Chen SJ,He L,Zhou K,Zou LK

更新日期：2018-06-12 00:00:00

abstract：:Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natur...

journal_title：AMB Express

authors： Roth T,Beer B,Pick A,Sieber V

更新日期：2017-12-01 00:00:00

abstract：:Recombinant antibodies are used with great success in many different diagnostic and therapeutic applications. A variety of protein expression systems are available, but nowadays almost all therapeutic antibodies are produced in mammalian cell lines due to their complex structure and glycosylation requirements. However...

journal_title：AMB Express

authors： Blanco-Toribio A,Álvarez-Cienfuegos A,Sainz-Pastor N,Merino N,Compte M,Sanz L,Blanco FJ,Álvarez-Vallina L

更新日期：2015-12-01 00:00:00

abstract：:Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T. curvata DSM43183 revealed two genes coding for putative polyester hydrolases Tcur1278 and Tcur0390 sharing...

journal_title：AMB Express

authors： Wei R,Oeser T,Then J,Kühn N,Barth M,Schmidt J,Zimmermann W

更新日期：2014-06-03 00:00:00

abstract：:Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the...

journal_title：AMB Express

authors： Shui Y,Guan ZB,Liu GF,Fan LM

更新日期：2020-01-14 00:00:00

abstract：:The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

journal_title：AMB Express

authors： Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

更新日期：2016-12-01 00:00:00

abstract：:Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella living...

journal_title：AMB Express

authors： Tajima T,Fuki K,Kataoka N,Kudou D,Nakashimada Y,Kato J

更新日期：2013-12-05 00:00:00

abstract：:Hydrogen gas represents a promising alternative energy source to dwindling fossil fuel reserves, as it carries the highest energy per unit mass and its combustion results in the release of water vapour as only byproduct. The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hyd...

journal_title：AMB Express

authors： Mohr T,Aliyu H,Biebinger L,Gödert R,Hornberger A,Cowan D,de Maayer P,Neumann A

更新日期：2019-12-23 00:00:00

abstract：:Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the...

journal_title：AMB Express

authors： Shen Y,Li J,Xiang J,Wang J,Yin K,Liu Q

更新日期：2019-07-27 00:00:00

abstract：:Green alga Chlamydomonas reinhardtii has gained interest as a sustainable resource because it can be easily grown using CO2 as a carbon source owing to its high CO2 assimilating activity. Although the robustness of the cell wall of C. reinhardtii makes it difficult to extract its intracellular products, such property ...

journal_title：AMB Express

authors： Nakanishi A,Iritani K,Sakihama Y,Ozawa N,Mochizuki A,Watanabe M

更新日期：2020-06-10 00:00:00

abstract：:Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

journal_title：AMB Express

authors： Ng TW,Chan WL,Lai KM

更新日期：2017-12-01 00:00:00

abstract：:Although high levels of staphylococcal phenol-soluble modulins (PSMs) in clinical methicillin-resistant Staphylococcus aureus (MRSA) has been shown to correlate with bacterial virulence, the PSMs expression in foodborne Staphylococcus aureus (S. aureus), as well as its association with staphylococcal food poisoning (S...

journal_title：AMB Express

authors： Wu X,Yang M,Fang X,Zhen S,Zhang J,Yang X,Qiao L,Yang Y,Zhang C

更新日期：2018-11-22 00:00:00

abstract：:Pectin is a structural heteropolysaccharide of the primary cell walls of plants and as such is a significant fraction of agricultural waste residues that is currently insufficiently used. Its main component, D-galacturonic acid, is an attractive substrate for bioconversion. The complete metabolic pathway is present in...

journal_title：AMB Express

authors： Sloothaak J,Schilders M,Schaap PJ,de Graaff LH

更新日期：2014-08-23 00:00:00

abstract：:Geniposide is an iridoid glycoside, which is abundant in Gardeniae Fructus. Despite the various pharmaceutical effects of geniposide on a human body, its hydrolysis into a smaller molecule, genipin, by β-glucosidase produced by bacteria in the intestines is particularly important to improve geniposide uptake into the ...

journal_title：AMB Express

authors： Kim YS,Lee CJ,Ma JY

更新日期：2017-12-01 00:00:00

abstract：:Biological technologies for recycling rare metals, which are essential for high-tech products, have attracted much attention because they could prove to be more environmentally friendly and energy-saving than other methods. We have developed biological recycling technologies by cell surface engineering for the selecti...

journal_title：AMB Express

authors： Ito R,Kuroda K,Hashimoto H,Ueda M

更新日期：2016-12-01 00:00:00

abstract：: Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-treatment through adsorption on activated carbon, 25%-50% (v/v) pre-treated vinasse was utilized leading t...

journal_title：AMB Express

authors： Bhattacharyya A,Pramanik A,Maji SK,Haldar S,Mukhopadhyay UK,Mukherjee J

更新日期：2012-07-09 00:00:00

abstract：:Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. Increasing interest in controlling water-borne pathogens in water resources evidenced by a large number of recent publications clearly attests to the need for studies that synthesize knowl...

journal_title：AMB Express

authors： Pandey PK,Kass PH,Soupir ML,Biswas S,Singh VP

更新日期：2014-06-28 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

journal_title：AMB Express

authors： Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

更新日期：2020-12-11 00:00:00

abstract：:Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

journal_title：AMB Express

authors： Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

更新日期：2018-08-06 00:00:00

abstract：:Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...

journal_title：AMB Express

authors： Kang TS,Korber DR,Tanaka T

更新日期：2013-01-31 00:00:00

abstract：:Aspergillus aculeatus β-glucosidase 1 (AaBGL1), which promotes cellulose hydrolysis by Trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orthologue in A. niger (AnBGL) to elucidate advantages of recombinant AaBGL1 (rAaBGL1) for synergistic effect on Trichoderma enz...

journal_title：AMB Express

authors： Baba Y,Sumitani JI,Tani S,Kawaguchi T

更新日期：2015-01-24 00:00:00

abstract：:The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred...

journal_title：AMB Express

authors： Xu L,Gao J,Guo L,Yu H

更新日期：2020-01-18 00:00:00

abstract：:This study investigated the effects of the rumen fungus Piromyces sp. CN6 CGMCC 14449 as a silage additive on the fermentation quality, nutrient composition and in vitro digestibility of whole crop maize silage. Whole crop maize served as the silage material and was vacuum packed in polyethylene bags. Three ensiling t...

journal_title：AMB Express

authors： Wang D,Zhao C,Liu S,Zhang T,Yao J,Cao Y

更新日期：2019-07-29 00:00:00

abstract：:The zygomycete fungus Lichtheimia ramosa H71D, isolated from sugarcane bagasse compost, was identified by applying phylogenetic analysis based on the DNA sequence of the Internal Transcribed Spacer (ITS), and subsequent secondary structure analysis of ITS2. L. ramosa H71D was able to grow over a wide range of temperat...

journal_title：AMB Express

authors： Alvarez-Zúñiga MT,Santiago-Hernández A,Rodríguez-Mendoza J,Campos JE,Pavón-Orozco P,Trejo-Estrada S,Hidalgo-Lara ME

更新日期：2017-11-02 00:00:00

abstract：: In this study manganese peroxidase (MnP) enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid media and a simplified process was established for the purification of extracellular enzymes.Five lignin degra...

journal_title：AMB Express

authors： Järvinen J,Taskila S,Isomäki R,Ojamo H

更新日期：2012-11-29 00:00:00

abstract：:Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analys...

journal_title：AMB Express

authors： Majeed A,Majeed M,Thajuddin N,Arumugam S,Ali F,Beede K,Adams SJ,Gnanamani M

更新日期：2019-05-29 00:00:00