Expression and regulation of phenol-soluble modulins and enterotoxins in foodborne Staphylococcus aureus.

Abstract:

:Although high levels of staphylococcal phenol-soluble modulins (PSMs) in clinical methicillin-resistant Staphylococcus aureus (MRSA) has been shown to correlate with bacterial virulence, the PSMs expression in foodborne Staphylococcus aureus (S. aureus), as well as its association with staphylococcal food poisoning (SFP) was not yet clear. We collected a panel of 350 foodborne and 127 clinic-derived S. aureus strains and compared their PSMs expression. Overall, foodborne strains exhibited higher PSMs than clinical isolates, indicating a potential pathological significance of PSMs in staphylococcal food contamination. Furthermore, PSMs expression and staphylococcal enterotoxins (SEs) levels in relation to antibiotic sensitive and resistant strains were analysed. While the co-expression of PSMs and SEs was confirmed, one typical foodborne strain simultaneously yielding PSMs, SEB and SED was selected. By comparing this wildtype strain to a series of gene-deficient mutants, we concluded that PSMs and SEs expressions both relied on staphylococcal accessory regulator A initiation in the early stage of accessory gene regulator control, yet their succedent regulations differentiated to RNAIII-dependent and independent, respectively. These data provided preliminary insight into PSMs and SEs expression in foodborne S. aureus, and may guide the further studies on PSMs effects in SFP.

journal_name

AMB Express

journal_title

AMB Express

authors

Wu X,Yang M,Fang X,Zhen S,Zhang J,Yang X,Qiao L,Yang Y,Zhang C

doi

10.1186/s13568-018-0717-x

subject

Has Abstract

pub_date

2018-11-22 00:00:00

pages

187

issue

1

issn

2191-0855

pii

10.1186/s13568-018-0717-x

journal_volume

8

pub_type

杂志文章
  • (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB') from fatty acid degradation operon of Ralstonia eutropha H16.

    abstract::In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB', gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been heterologously expressed in Escherichia coli, purified as protein possessing a His-Tag and initially characterized. FadB' is...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0069-0

    authors: Volodina E,Steinbüchel A

    更新日期:2014-08-28 00:00:00

  • Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells.

    abstract::Cardiac arrhythmia is an irregular heart rhythm that can lead to serious heart conditions and various organ disorders, and may cause sudden cardiac death. Catalpol belongs to the iridoid glycoside family and is highly abundant in Rehmannia glutinosa Libosch. The study included five groups such as group I (normal contr...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00986-9

    authors: Yang L,Feng X,Li Y,Zhang S,Ying Y

    更新日期:2020-03-20 00:00:00

  • Cloning and expression of a novel α-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68.

    abstract::The discovery and creation of biocatalysts for plant biomass conversion are essential for industrial demand and scientific research of the plant cell wall. α-1,2 and α-1,3-L-arabinofuranosidases are debranching enzymes that catalyzing hydrolytic release of α-L-arabinofuranosyl residues in plant cell wall. Gene databas...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0577-4

    authors: Hu Y,Yan X,Zhang H,Liu J,Luo F,Cui Y,Wang W,Zhou Y

    更新日期:2018-04-02 00:00:00

  • Overexpression of the Aspergillus niger GatA transporter leads to preferential use of D-galacturonic acid over D-xylose.

    abstract::Pectin is a structural heteropolysaccharide of the primary cell walls of plants and as such is a significant fraction of agricultural waste residues that is currently insufficiently used. Its main component, D-galacturonic acid, is an attractive substrate for bioconversion. The complete metabolic pathway is present in...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0066-3

    authors: Sloothaak J,Schilders M,Schaap PJ,de Graaff LH

    更新日期:2014-08-23 00:00:00

  • Nigella A ameliorates inflammation and intestinal flora imbalance in DSS induced colitis mice.

    abstract::Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. How...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01114-3

    authors: Hu X,Xu N,Yang X,Hu X,Zheng Y,Zhang Q

    更新日期:2020-10-04 00:00:00

  • Ammonium nitrate regulated the color characteristic changes of pigments in Monascus purpureus M9.

    abstract::Monascus pigments (MPs) with different color characteristics, produced by submerged fermentation of Monascus purpureus M9, have potential application in food industry. In the present study, the effects and regulatory mechanisms of ammonium nitrate (AN) on the color characteristics of MPs were investigated. The concent...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01165-6

    authors: Chen D,Wang Y,Chen M,Fan P,Li G,Wang C

    更新日期:2021-01-04 00:00:00

  • High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.

    abstract::Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitiv...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01163-8

    authors: Liu BY,Gao B,Liu MZ,Zhang TT,Liu BS,Chen ZL

    更新日期:2020-12-11 00:00:00

  • High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization.

    abstract::The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is comparatively simple, well characterized and can be manipulated easily...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0155-y

    authors: Kangwa M,Yelemane V,Polat AN,Gorrepati KD,Grasselli M,Fernández-Lahore M

    更新日期:2015-12-01 00:00:00

  • Microbial production of Propionic and Succinic acid from Sorbitol using Propionibacterium acidipropionici.

    abstract::Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0095-6

    authors: Duarte JC,Valença GP,Moran PJ,Rodrigues JA

    更新日期:2015-02-20 00:00:00

  • Natural antibacterial agents from arid-region pretreated lignocellulosic biomasses and extracts for the control of lactic acid bacteria in yeast fermentation.

    abstract::Bacterial contamination is one of the major challenges faced by yeast fermentation industries as the contaminating microorganisms produce lactic acid and acetic acid, which reduces the viability of yeast, and hence fermentation yields. The primary bacterial contaminants of yeast fermentations are lactic acid bacteria ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0654-8

    authors: Haris S,Fang C,Bastidas-Oyanedel JR,Prather KJ,Schmidt JE,Thomsen MH

    更新日期:2018-08-06 00:00:00

  • Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus.

    abstract::In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) an...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0433-y

    authors: Mohd Zin NB,Mohamad Yusof B,Oslan SN,Wasoh H,Tan JS,Ariff AB,Halim M

    更新日期:2017-12-01 00:00:00

  • iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae.

    abstract::Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a swine respiratory disease with an important impact around the world either as a single infection or part of the porcine respiratory disease complex. The data of interaction between hosts and pathogens has becoming more crucial for exploration...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01057-9

    authors: Zhu R,Bao C,Liu B,Xiao J,Sun C,Feng X,Langford PR,Li Y,Lei L

    更新日期:2020-07-06 00:00:00

  • Recovery of platinum(0) through the reduction of platinum ions by hydrogenase-displaying yeast.

    abstract::Biological technologies for recycling rare metals, which are essential for high-tech products, have attracted much attention because they could prove to be more environmentally friendly and energy-saving than other methods. We have developed biological recycling technologies by cell surface engineering for the selecti...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-016-0262-4

    authors: Ito R,Kuroda K,Hashimoto H,Ueda M

    更新日期:2016-12-01 00:00:00

  • Thermostabilization of the uronate dehydrogenase from Agrobacterium tumefaciens by semi-rational design.

    abstract::Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natur...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0405-2

    authors: Roth T,Beer B,Pick A,Sieber V

    更新日期:2017-12-01 00:00:00

  • Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera.

    abstract::The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01143-y

    authors: Kaur N,Arora DS

    更新日期:2020-11-11 00:00:00

  • Purification and characterization of neutral protease from Aspergillus oryzae Y1 isolated from naturally fermented broad beans.

    abstract::The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broa...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0611-6

    authors: Ao XL,Yu X,Wu DT,Li C,Zhang T,Liu SL,Chen SJ,He L,Zhou K,Zou LK

    更新日期:2018-06-12 00:00:00

  • Development and testing of a 3D-printable polylactic acid device to optimize a water bioremediation process.

    abstract::In the present work, a remediation bioprocess based on the use of a native isolate of Chlorella vulgaris immobilized in an alginate matrix inside a polylactic acid (PLA) device is proposed. This microalga immobilized in alginate beads was previously shown to be useful for the reduction of several chemical and microbia...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-01081-9

    authors: Marconi PL,Trentini A,Zawoznik M,Nadra C,Mercadé JM,Sánchez Novoa JG,Orozco D,Groppa MD

    更新日期:2020-08-15 00:00:00

  • Improved conversion of ginsenoside Rb1 to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase.

    abstract::Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production. The hydrolytic pathway from ginsenoside Rb1 to compound K via Rd...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0487-x

    authors: Shin KC,Choi HY,Seo MJ,Oh DK

    更新日期:2017-10-04 00:00:00

  • Importance of stress-response genes to the survival of airborne Escherichia coli under different levels of relative humidity.

    abstract::Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-017-0376-3

    authors: Ng TW,Chan WL,Lai KM

    更新日期:2017-12-01 00:00:00

  • The regulatory effect of Lactobacillus rhamnosus GG on T lymphocyte and the development of intestinal villi in piglets of different periods.

    abstract::The maturation and development of T cells were not completed until T cells were selected in thymus. It was not until the early 1960s that j.f.a.p. discovered the importance of thymus in T cell development. Twelve healthy piglets were randomly divided into two groups, the experimental group (LGG group) and the control ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-020-00980-1

    authors: Shonyela SM,Feng B,Yang W,Yang G,Wang C

    更新日期:2020-04-17 00:00:00

  • Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii.

    abstract::Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0057-4

    authors: Almaraz-Delgado AL,Flores-Uribe J,Pérez-España VH,Salgado-Manjarrez E,Badillo-Corona JA

    更新日期:2014-08-13 00:00:00

  • Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase.

    abstract::Clean coal technologies (e.g. coal biosolubilization) are of essential value, especially in Europe, where coal is the national wealth and other energy sources like crude oil are not available. Fusarium oxysporum LOCK 1134, the strain isolated from brown coal, efficiently biosolubilizes lignite. The obtained liquefied ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-018-0669-1

    authors: Kwiatos N,Jędrzejczak-Krzepkowska M,Strzelecki B,Bielecki S

    更新日期:2018-08-22 00:00:00

  • Effects of Piromyces sp. CN6 CGMCC 14449 on fermentation quality, nutrient composition and the in vitro degradation rate of whole crop maize silage.

    abstract::This study investigated the effects of the rumen fungus Piromyces sp. CN6 CGMCC 14449 as a silage additive on the fermentation quality, nutrient composition and in vitro digestibility of whole crop maize silage. Whole crop maize served as the silage material and was vacuum packed in polyethylene bags. Three ensiling t...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0846-x

    authors: Wang D,Zhao C,Liu S,Zhang T,Yao J,Cao Y

    更新日期:2019-07-29 00:00:00

  • Characterization of Aspergillus aculeatus β-glucosidase 1 accelerating cellulose hydrolysis with Trichoderma cellulase system.

    abstract::Aspergillus aculeatus β-glucosidase 1 (AaBGL1), which promotes cellulose hydrolysis by Trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orthologue in A. niger (AnBGL) to elucidate advantages of recombinant AaBGL1 (rAaBGL1) for synergistic effect on Trichoderma enz...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0090-3

    authors: Baba Y,Sumitani JI,Tani S,Kawaguchi T

    更新日期:2015-01-24 00:00:00

  • NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level.

    abstract::A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phosphate dehydrogenase...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-014-0081-4

    authors: Knudsen JD,Carlquist M,Gorwa-Grauslund M

    更新日期:2014-10-30 00:00:00

  • Large-scale microcarrier culture of HEK293T cells and Vero cells in single-use bioreactors.

    abstract::Gene therapy and viral vaccine are becoming attractive therapeutic options for the treatment of different malignant diseases. Viral vector productions are often using static culture vessels and small volume stainless steel bioreactors (SSB). However, the yield of each vessel can be relatively low and multiple vessels ...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0794-5

    authors: Yang J,Guertin P,Jia G,Lv Z,Yang H,Ju D

    更新日期:2019-05-24 00:00:00

  • Influence of oxygen on NADH recycling and oxidative stress resistance systems in Lactobacillus panis PM1.

    abstract::Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/2191-0855-3-10

    authors: Kang TS,Korber DR,Tanaka T

    更新日期:2013-01-31 00:00:00

  • The two-component system CepRS regulates the cephamycin C biosynthesis in Streptomyces clavuligerus F613-1.

    abstract::During industrial fermentation, Streptomyces clavuligerus F613-1 simultaneously produces primary product clavulanic acid (CA) and cephamycin C. The cephamycin C biosynthetic gene cluster and pathway have been basically elucidated and the CcaR positive regulator was found to control the cephamycin genes expression. How...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0844-z

    authors: Fu J,Qin R,Zong G,Zhong C,Zhang P,Kang N,Qi X,Cao G

    更新日期:2019-07-27 00:00:00

  • Mixed alcoholic fermentation of Schizosaccharomyces pombe and Lachancea thermotolerans and its influence on mannose-containing polysaccharides wine Composition.

    abstract::This study researched the winemaking performance of new biotechnology involving the cooperation of Lachancea and Schizosaccharomyces genera in the production of wine. In all fermentations where Lachancea thermotolerans was involved, higher lactic acid concentrations appeared, while all fermentations where Schizosaccha...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-019-0738-0

    authors: Benito Á,Calderón F,Benito S

    更新日期:2019-02-02 00:00:00

  • Optimisation of enzymatic hydrolysis of cassava peel to produce fermentable sugars.

    abstract::Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...

    journal_title:AMB Express

    pub_type: 杂志文章

    doi:10.1186/s13568-015-0146-z

    authors: Bayitse R,Hou X,Bjerre AB,Saalia FK

    更新日期:2015-12-01 00:00:00