Enhancement of active compound, genipin, from Gardeniae Fructus using immobilized glycosyl hydrolase family 3 β-glucosidase from Lactobacillus antri.

abstract：:Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30°C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 ± 5...

journal_title：AMB Express

authors： Duarte JC,Valença GP,Moran PJ,Rodrigues JA

更新日期：2015-02-20 00:00:00

abstract：:Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L...

journal_title：AMB Express

authors： Ece S,Lambertz C,Fischer R,Commandeur U

更新日期：2017-12-01 00:00:00

abstract：:High temperature causes ubiquitous environmental stress to microorganisms, but studies have not fully explained whether and to what extent heat shock would affect genome stability. Hence, this study explored heat-shock-induced genomic alterations in the yeast Saccharomyces cerevisiae. Using genetic screening systems a...

journal_title：AMB Express

authors： Shen L,Wang YT,Tang XX,Zhang K,Wang PM,Sui Y,Zheng DQ

更新日期：2020-08-17 00:00:00

abstract：:Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have...

journal_title：AMB Express

authors： Almaraz-Delgado AL,Flores-Uribe J,Pérez-España VH,Salgado-Manjarrez E,Badillo-Corona JA

更新日期：2014-08-13 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the prese...

journal_title：AMB Express

authors： Lee JH,Kim YG,Gwon G,Wood TK,Lee J

更新日期：2016-12-01 00:00:00

abstract：:During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the S...

journal_title：AMB Express

authors： El-Rotail AAMM,Zhang L,Li Y,Liu SP,Shi GY

更新日期：2017-12-01 00:00:00

abstract：:Poly-3-hydroxybutyrate (PHB), the most abundant type of polyhydroxyalkanoates (PHA) is synthesized inside a variety of microorganisms as a primary candidate for industrial PHB production. Lipophilic dyes such as Nile red and BODIPY have been used to quantify intracellular PHB, but their uses have often been limited in...

journal_title：AMB Express

authors： Choi JE,Na HY,Yang TH,Rhee SK,Song JK

更新日期：2015-12-01 00:00:00

abstract：: Biocathode MFCs using microorganisms as catalysts have important advantages in lowering cost and improving sustainability. Electrode materials and microbial synergy determines biocathode MFCs performance. In this study, four materials, granular activated carbon (GAC), granular semicoke (GS), granular graphite (GG) an...

journal_title：AMB Express

authors： Sun Y,Wei J,Liang P,Huang X

更新日期：2012-03-29 00:00:00

abstract：:Poly-gamma-glutamic acid (PGA) is a promising bio-based polymer that shares many functions with poly (acrylic acid) and its derivatives. Thus, technologies for efficient production and molecular size control of PGA are required to expand the application of this useful biopolymer. In Bacillus strains, PGA is synthesize...

journal_title：AMB Express

authors： Sawada K,Araki H,Takimura Y,Masuda K,Kageyama Y,Ozaki K,Hagihara H

更新日期：2018-07-03 00:00:00

abstract：:Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressu...

journal_title：AMB Express

authors： Sakudo A,Yagyu Y

更新日期：2021-01-09 00:00:00

abstract：:Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analys...

journal_title：AMB Express

authors： Majeed A,Majeed M,Thajuddin N,Arumugam S,Ali F,Beede K,Adams SJ,Gnanamani M

更新日期：2019-05-29 00:00:00

abstract：:Origanum vulgare L. (Lamiaceae) is a widespread flavoring culinary and medicinal herb. The present study aimed at investigating the antimicrobial activity of Origanum vulgare (OV) essential oil (EO) through illustrating its biostatic, biocidal and the dynamics of the biocidal activity against 11 different microorganis...

journal_title：AMB Express

authors： Fikry S,Khalil N,Salama O

更新日期：2019-03-26 00:00:00

abstract：:The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male broilers (Ross 308) of 1 day ag...

journal_title：AMB Express

authors： Kumbhar S,Khan AZ,Parveen F,Nizamani ZA,Siyal FA,El-Hack MEA,Gan F,Liu Y,Hamid M,Nido SA,Huang K

更新日期：2018-07-10 00:00:00

abstract：:Herbicides are important tools for weed control in modern agriculture. In the search for potential herbicidal natural products from fungal species, harzianum A and B were identified from the biofertilizer fungus, Trichoderma brevicompactum. In the phytotoxicity assays on the dicot species Brassica chinensis, harzianum...

journal_title：AMB Express

authors： Yin M,Fasoyin OE,Wang C,Yue Q,Zhang Y,Dun B,Xu Y,Zhang L

更新日期：2020-07-01 00:00:00

abstract：:The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...

journal_title：AMB Express

authors： Ibrahim MHA,Lebbe L,Willems A,Steinbüchel A

更新日期：2016-12-01 00:00:00

abstract：: Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus ha...

journal_title：AMB Express

authors： Martínez CP,Echeverri C,Florez JC,Gaitan AL,Góngora CE

更新日期：2012-03-30 00:00:00

abstract：:The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA...

journal_title：AMB Express

authors： Hu Z,Ma D,Niu H,Chang J,Yu J,Tong Q,Li S

更新日期：2021-01-06 00:00:00

abstract：:As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11...

journal_title：AMB Express

authors： Zhou Y,He P,Xu Y,Liu Q,Yang Y,Liu S

更新日期：2017-09-29 00:00:00

abstract：:Thermomonospora curvata is a thermophilic actinomycete phylogenetically related to Thermobifida fusca that produces extracellular hydrolases capable of degrading synthetic polyesters. Analysis of the genome of T. curvata DSM43183 revealed two genes coding for putative polyester hydrolases Tcur1278 and Tcur0390 sharing...

journal_title：AMB Express

authors： Wei R,Oeser T,Then J,Kühn N,Barth M,Schmidt J,Zimmermann W

更新日期：2014-06-03 00:00:00

abstract：:Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticidal process, chitinases are suggested to perforate the peritrophic membrane barrier to facilitate the invasion of the Cry proteins int...

journal_title：AMB Express

authors： Honda S,Kunii T,Nohara K,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2017-12-01 00:00:00

abstract：:This study was demonstrated with a coculture fermentation system using sugar beet pulp (SBP) as a carbon source combining the cellulose-degrading bacterium Clostridium cellulovorans with microbial flora of methane production (MFMP) for the direct conversion of cellulosic biomass to methane (CH4). The MFMP was taken fr...

journal_title：AMB Express

authors： Tomita H,Okazaki F,Tamaru Y

更新日期：2019-02-18 00:00:00

abstract：:Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this ext...

journal_title：AMB Express

authors： Roberts DP,Selmer K,Lupitskyy R,Rice C,Buyer JS,Maul JE,Lakshman DK,DeSouza J

更新日期：2021-01-06 00:00:00

abstract：:Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate conce...

journal_title：AMB Express

authors： Silva HR,Prete CEC,Zambrano F,de Mello VH,Tischer CA,Andrade DS

更新日期：2016-03-01 00:00:00

abstract：:Here, we report a novel protein elicitor from Bacillus subtilis BU412 which could cause hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco. The purification was executed by ion-exchange and size exclusion chromatography. The target band on SDS-PAGE was analyzed by mass spectrometry, and the...

journal_title：AMB Express

authors： Shen Y,Li J,Xiang J,Wang J,Yin K,Liu Q

更新日期：2019-07-27 00:00:00

abstract：:Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In ...

journal_title：AMB Express

authors： Kitahara N,Morisaka H,Aoki W,Takeda Y,Shibasaki S,Kuroda K,Ueda M

更新日期：2015-12-01 00:00:00

abstract：:The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees rev...

journal_title：AMB Express

authors： Oueslati M,Mulet M,Zouaoui M,Chandeysson C,Lalucat J,Hajlaoui MR,Berge O,García-Valdés E,Sadfi-Zouaoui N

更新日期：2020-11-01 00:00:00

abstract：:The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial ...

journal_title：AMB Express

authors： Kaur N,Arora DS

更新日期：2020-11-11 00:00:00

abstract：:With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae...

journal_title：AMB Express

authors： Eibl JK,Corcoran JD,Senhorinho GN,Zhang K,Hosseini NS,Marsden J,Laamanen CA,Scott JA,Ross GM

更新日期：2014-03-26 00:00:00

abstract：:Trace metals are required in many cellular processes in bacteria but also induce toxic effects to cells when present in excess. As such, various forms of adaptive responses towards extracellular trace metal ions are essential for the survival and fitness of bacteria in their environment. A soil Pseudomonas putida, str...

journal_title：AMB Express

authors： Chong TM,Yin WF,Chen JW,Mondy S,Grandclément C,Faure D,Dessaux Y,Chan KG

更新日期：2016-12-01 00:00:00

abstract：:Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. How...

journal_title：AMB Express

authors： Hu X,Xu N,Yang X,Hu X,Zheng Y,Zhang Q

更新日期：2020-10-04 00:00:00