Abstract:
:Fusicocca-2,10(14)-diene (FCdiene) is a diterpene which is interesting as a precursor of the anti-cancer drug fusicoccin A and therefore for pharmaceutical applications. Production of FCdiene using a genetically modified Saccharomyces cerevisiae has been previously demonstrated with batch cultivations with a product concentration up to 10 mg/L. However, it is widely known that fed-batch processes can significantly improve product titer in yeast fermentations. This study focuses on the establishment of fed-batch fermentation for FCdiene production because fed-batch cultivations using FeedBeads® indicated that limiting glucose supply could increase yields of biomass (1.07 gCDW/gGlucose instead of 0.20 gCDW/gGlucose) and FCdiene (21.54 mgFCdiene/gGlucose instead of 9.74 mgFCdiene/gGlucose) in shake flask scale and may have implications for larger scale processes. We implemented a new exponential glucose feed profile in a 1.8 L stirred tank reactor. This reduced overfeeding and the consequent, ethanol production. As a result improvements in cell concentrations up to 246% could be achieved and FCdiene yield increased up to 2.8X in the first 28 h. FCdiene concentration reached 161 mg/L and 320 mg/L at 44 h. Fed-batch and batch mode were combined to examine dynamics of bi-modal cultivation where a fed-batch phase was used for biomass production and a batch phase used for FCdiene production potentially supported by ethanol consumption as reported on production of betulinic acid. The present study highlights the potential of process development improvements which increase high-value heterologous diterpene yields from S. cerevisiae.
journal_name
AMB Expressjournal_title
AMB Expressauthors
Halka LM,Nowacki C,Kleinschmidt A,Koenen K,Wichmann Rdoi
10.1186/s13568-018-0662-8subject
Has Abstractpub_date
2018-08-22 00:00:00pages
132issue
1issn
2191-0855pii
10.1186/s13568-018-0662-8journal_volume
8pub_type
杂志文章相关文献
AMB Express文献大全abstract::Efficient methods to achieve the safe decontamination of agricultural products are needed. Here, we investigated the decontamination of citrus fruits to test the antifungal potential of a novel non-thermal gas plasma apparatus, termed a roller conveyer plasma instrument. This instrument generates an atmospheric pressu...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-020-01177-2
更新日期:2021-01-09 00:00:00
abstract::Sweet potato vine (SPV) is an abundant agricultural waste, which is easy to obtain at low cost and has the potential to produce clean energy via anaerobic digestion (AD). The main objectives of this study were to reveal methane production and process stability of SPV and the mixtures with animal manure under various t...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0572-9
更新日期:2018-03-22 00:00:00
abstract::Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS m...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0620-5
更新日期:2018-05-28 00:00:00
abstract::Bacillus thuringiensis is a Gram-positive soil bacterium that is known to be a bacterial biopesticide that produces insecticidal proteins called crystal proteins (Cry). In the insecticidal process, chitinases are suggested to perforate the peritrophic membrane barrier to facilitate the invasion of the Cry proteins int...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-017-0352-y
更新日期:2017-12-01 00:00:00
abstract:: Biosurfactants are amphiphilic molecules that have both hydrophilic and hydrophobic moieties which partition preferentially at the interfaces such as liquid/liquid, gas/liquid or solid/liquid interfaces. Such characteristics enable emulsifying, foaming, detergency and dispersing properties. Their low toxicity and env...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/2191-0855-1-5
更新日期:2011-03-28 00:00:00
abstract::Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/2191-0855-3-10
更新日期:2013-01-31 00:00:00
abstract::Although high levels of staphylococcal phenol-soluble modulins (PSMs) in clinical methicillin-resistant Staphylococcus aureus (MRSA) has been shown to correlate with bacterial virulence, the PSMs expression in foodborne Staphylococcus aureus (S. aureus), as well as its association with staphylococcal food poisoning (S...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0717-x
更新日期:2018-11-22 00:00:00
abstract::Aglycon protopanaxatriol (APPT) has valuable pharmacological effects such as anti-inflammatory and anti-stress activities. However, the complete conversion of all typical glycosylated protopanaxatriol ginsenosides to APPT has not been achieved to date. β-Glycosidase from the hyperthermophilic bacterium Dictyoglomus tu...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0543-1
更新日期:2018-01-24 00:00:00
abstract::With the aim to develop biocatalysts for enhanced hydrolysis of (hemi)cellulose into monosaccharides, random diversity by directed evolution was introduced in the gene coding for the endo-β-1,4-glucanase from Streptomyces sp. G12 which had been recombinantly expressed in Escherichia coli and named rCelStrep. The main ...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0602-7
更新日期:2018-05-05 00:00:00
abstract::Mycolata form a group of Gram-positive bacteria with unique cell envelope structures that are known for their high tolerance against antibiotics and both aromatic and aliphatic hydrocarbons. An important part of the unique surface structure of the mycolata is the presence of long chain α-alkyl-β-hydroxy fatty acids, t...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-016-0241-9
更新日期:2016-12-01 00:00:00
abstract::Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify a...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-019-0943-x
更新日期:2020-01-14 00:00:00
abstract::The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-020-0947-6
更新日期:2020-01-18 00:00:00
abstract::In this paper, we demonstrate that the antimicrobial activity of L. plantarum PBS067 strain against antagonist microorganisms was mediated by the production of a bacteriocin-like compound secreted at the stationary phase of the growth. The novel bacteriocin-like compound, designed plantaricin P1053, was identified by ...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-019-0813-6
更新日期:2019-06-17 00:00:00
abstract::The basidiospores of most Agaricomycetes are ballistospores. They are propelled off from their basidia at maturity when Buller's drop develops at high humidity at the hilar spore appendix and fuses with a liquid film formed on the adaxial side of the spore. Spores are catapulted into the free air space between hymenia...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-017-0326-0
更新日期:2017-12-01 00:00:00
abstract::Enzymatic hydrolysis of cassava peels was evaluated using cellulase and beta-glucanase enzymes and their mixtures at three different enzyme loadings with time. The pH of the medium used for hydrolysis was 5 and the temperature was 50 °C. The efficiency of the hydrolysis using beta-glucanase was better than cellulase a...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-015-0146-z
更新日期:2015-12-01 00:00:00
abstract::Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-019-0944-9
更新日期:2020-01-14 00:00:00
abstract::With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/2191-0855-4-7
更新日期:2014-03-26 00:00:00
abstract::Every year about 20% of the globally produced meat gets lost due to microbial spoilage. Nevertheless, the demand for processed meat is constantly rising and producers are searching for novel strategies to reduce microbial contaminations in their products. In the present study, we evaluated the applicability of alkylpy...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0583-6
更新日期:2018-04-03 00:00:00
abstract::An exhaustive screening program was applied for scoring a promising L-asparaginase producing-isolate. The recovered isolate was identified biochemically and molecularly and its L-asparaginase productivity was optimized experimentally and by Response Surface Methodology. The produced enzyme was characterized experiment...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-020-01005-7
更新日期:2020-04-15 00:00:00
abstract:: Biocathode MFCs using microorganisms as catalysts have important advantages in lowering cost and improving sustainability. Electrode materials and microbial synergy determines biocathode MFCs performance. In this study, four materials, granular activated carbon (GAC), granular semicoke (GS), granular graphite (GG) an...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/2191-0855-2-21
更新日期:2012-03-29 00:00:00
abstract::Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liqui...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0731-z
更新日期:2019-01-07 00:00:00
abstract::Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate conce...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-016-0180-5
更新日期:2016-03-01 00:00:00
abstract::The poly(3-hydroxybutyrate), PHB, accumulating thermophilic strain MW9(T), isolated from an aerobic organic waste treatment plant, was characterized by detailed physiological and phylogenetic studies. The strain is a Gram-stain-negative, rod shaped, non-spore forming member of Alphaproteobacteria. It shows optimum gro...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-016-0209-9
更新日期:2016-12-01 00:00:00
abstract::Crude enzyme from Geotrichum candidum S12 exhibited high activity towards hexanol at pH 4.0, distinguishing it from currently known enzymes. To identify the dominant enzyme contributing to this activity, the crude enzyme extract was separated into different fractions by ammonium sulfate precipitation, MonoQ anion-exch...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-016-0307-8
更新日期:2017-12-01 00:00:00
abstract::PCV3 capsid protein (Cap) is an important antigen for diagnosis and vaccine development. To achieve high-level expression of recombinant PCV3 Cap in Escherichia coli (E. coli), the gene of wild-type entire Cap (wt-eCap) was amplified from clinical samples, and three optimized entire Cap (opti-eCap) and one optimized C...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-019-0940-0
更新日期:2020-01-07 00:00:00
abstract::The zygomycete fungus Lichtheimia ramosa H71D, isolated from sugarcane bagasse compost, was identified by applying phylogenetic analysis based on the DNA sequence of the Internal Transcribed Spacer (ITS), and subsequent secondary structure analysis of ITS2. L. ramosa H71D was able to grow over a wide range of temperat...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-017-0494-y
更新日期:2017-11-02 00:00:00
abstract::The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to ass...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0668-2
更新日期:2018-08-28 00:00:00
abstract::The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male broilers (Ross 308) of 1 day ag...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-018-0641-0
更新日期:2018-07-10 00:00:00
abstract::Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolys...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-014-0036-9
更新日期:2014-04-25 00:00:00
abstract::In the present study, a methanogenic alkane-degrading (a mixture of C9 to C12n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over a...
journal_title:AMB Express
pub_type: 杂志文章
doi:10.1186/s13568-020-0956-5
更新日期:2020-02-01 00:00:00