Abstract:
OBJECTIVES:To evaluate the mRNA expression levels of cytokines interferon-γ, tumour necrosis factor-α, interleukin IL-1β, IL-10, and the chemokine CCL2/MCP-1, CCL4, and CXCR4 in the periapical interstitial fluid from root canal infections before and after bacterial load reduction in patients undergoing haematopoietic stem cell transplantation (HSCT). MATERIALS AND METHODS:The case group was composed of 10 patients undergoing HSCT, and our control group included 10 healthy patients. Clinical samples were taken from teeth with pulp necrosis. Three paper points were placed in the RCS and maintained for 2 min for microbial evaluation before cleaning and shaping procedures. After cleaning and drying the canal, three paper points were introduced into the root canal, passing passively through the root apex (2 mm) into the periapical tissues for 1 min. Samples were collected immediately after root canal cleaning and 7 days later (restrained root canal bacterial load) to characterize gene expression using real-time PCR. RESULTS:The results showed significantly reduction in the microbial load on day 7. An increased expression level of TNF-α and IFN-γ on day 7 in control and case groups was observed (p < 0.05). The mRNA levels of IL-1β and IL-10 in the pre-HSCT group increased in the samples from day 7 (p < 0.05). The chemokine CCL-2/MCP-1 was not detected in pre-HSCT group. Chemokine receptor CXCR4 levels increased in samples obtained from the day 7 in the control group (p < 0.05). CONCLUSIONS:Individuals undergoing HSTC presented similar cytokine and chemokine mRNA expression compared with healthy individuals. However, it was observed the total absence of mRNA MCP-1/CCL2 expression in those individuals undergoing HSCT. CLINICAL RELEVANCE:Patients undergoing HSCT are at higher risk of infection. No study has analysed the periapical immune responses to root canal infections in HSCT individuals.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Braga Diniz JM,Espaladori MC,E Souza Silva ME,de Brito LCN,Vieira LQ,Sobrinho APRdoi
10.1007/s00784-020-03448-5subject
Has Abstractpub_date
2020-07-14 00:00:00eissn
1432-6981issn
1436-3771pii
10.1007/s00784-020-03448-5pub_type
杂志文章abstract::Smoking is a causative factor in oral squamous cell carcinomas (SCC). Unfortunately, only poor data exist regarding the quality of life of smokers vs non-smokers with SCC. The purpose of this study is to show a correlation between variables for comprehensive interdisciplinary rehabilitation and better patient quality ...
journal_title:Clinical oral investigations
pub_type: 杂志文章,多中心研究
doi:10.1007/s00784-011-0631-y
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:This aimed to assess the potential role of chronic stress in saliva secretion, xerostomia, and oral health in a population attending a saliva clinic. MATERIALS AND METHODS:Data of 114 patients who met the inclusion criteria and completed all questionnaires were analyzed in this study. Participants completed ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2393-2
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Intermittent parathyroid hormone (PTH) exerts anabolic effects on bone and has been approved for osteoporosis therapy. The dual actions of PTH are mediated primarily through the parathyroid hormone 1 receptor (PTH1R). Upon ligand binding, PTH1R activates diverse signaling pathways, including cAMP/protein kin...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0985-4
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:To develop and evaluate predictive nomogram for extended duration of surgery in patients following mandibular third molars (M3M) removal. MATERIALS AND METHODS:A retrospectively observational study was performed and designed. A credible random split-sample method was used to divide data into training and va...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03499-8
更新日期:2020-08-10 00:00:00
abstract:OBJECTIVES:Analgesics are one of the most frequently used medicines. Self-medication and misuse have been described in the literature. The purpose of this study was to document analgesic (mis)use in a population seeking emergency dental treatment. MATERIAL AND METHODS:Patients consulting a dental emergency service wer...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2228-6
更新日期:2018-04-01 00:00:00
abstract:: ...
journal_title:Clinical oral investigations
pub_type: 信件
doi:10.1007/s00784-018-2619-3
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:Ventilator-associated pneumonia (VAP) is the most frequent hospital-acquired infections in intensive care units (ICU). In the bundle of care to prevent the VAP, the oral care is very important strategies, to decrease the oropharyngeal bacterial colonization and presence of causative bacteria of VAP. In view ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2289-6
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to investigate the role of hyperbaric oxygen (HBO) in the healing of teeth extraction sockets and in alveolar ridge preservation. This may provide an experimental basis for the widespread application of HBO in oral implantation. METHODS:A total of 32 beagle dogs were included i...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03117-2
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:The study evaluates the influence of two spacer settings and three resin luting materials on the marginal and internal fit of polymer-infiltrated ceramic network (PICN) material crowns manufactured using a complete digital workflow. METHODS:Optical impressions of fifty identical dies were performed using th...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03053-1
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:In the current literature, cone beam computed tomography (CBCT) seems to be more accurate in detecting apical lesions (AL) than two-dimensional radiographs. Cortical bone thickness might have an influence on AL visibility. Therefore, the purpose of the study was to directly compare the diagnostic accuracy of...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02843-x
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to compare the effect of one-stage full-mouth disinfection (FMD) and conventional quadrant scaling in four weekly sessions (QS) on periodontal clinical parameters and halitosis among individuals with advanced chronic periodontitis. MATERIALS AND METHODS:In this randomized cont...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-016-1959-0
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:Obesity is associated with periodontitis, but the mechanisms underlying this association have yet to be unraveled. The present investigation was to evaluate a common rat model, in which obesity is induced by high-fat, high-sucrose diet (HFSD), for its applicability in periodontal research. MATERIALS AND MET...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2514-y
更新日期:2019-02-01 00:00:00
abstract::The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers submitted to dental X-ray using two anatomic sites: buccal mucosa and lateral border of the tongue. A total of 15...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0345-6
更新日期:2010-12-01 00:00:00
abstract::The aim of this study was to investigate the different variables in mesio-vertically positioned impacted or erupted third molars in a group of volunteers in Turkey to determine the angular changes and levels of eruption radiographically using standardised panoramic radiographs. There were 213 volunteers with impacted ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-001-0144-1
更新日期:2002-06-01 00:00:00
abstract:OBJECTIVES:Targeted modifications of the bulk implant surfaces using bioactive agents provide a promising tool for improvement of the long-term bony and soft tissue integration of dental implants. In this study, we assessed the cellular responses of primary human gingival fibroblasts (HGF) to different surface modifica...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1784-5
更新日期:2017-01-01 00:00:00
abstract::The purpose of the present study was to decide whether composite resin or conventional glass ionomer cement should be preferred as a base material in endodontically treated premolars. Twelve extracted human maxillary premolars were mounted in a universal testing machine at a 35 degrees angle. Cuspal stiffness was dete...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s007840050049
更新日期:1998-06-01 00:00:00
abstract::The aims of this study were to evaluate clinically and microbiologically the effects of two resin-modified glass-ionomer cements (RMGICs) used as liners after incomplete dentine caries removal and to identify Streptococcus mutans and Streptococcus sobrinus strains isolated from dentine samples, before and after indire...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0304-2
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVES:To compare selective COX-2 inhibitors with ibuprofen in terms of analgesia, rescue medication consumption, and adverse effects after impacted third molar removal. MATERIALS AND METHODS:Electronic databases were searched. Single dose, double-blind, randomized, and controlled clinical trials comparing the ana...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-019-02910-3
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:The aim of the present retrospective cohort study was to assess the 5-year outcome and survival of secondary root canal treatments (2°RCT), exploring the influence of pre-, intra-, and post-operative variables. MATERIALS AND METHODS:One hundred thirty-two endodontically retreated teeth were radiographically...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2229-5
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:To assess dimensional changes following alveolar ridge preservation using bovine-derived xenograft with 10% collagen and collagen membrane compared to ridge preservation by means of bovine-derived xenograft particles and collagen membrane or spontaneous healing in posterior sites. MATERIALS AND METHODS:Fort...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-019-02979-w
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:The purpose of this laboratory study was to evaluate the influence of the attachment design and material on the retention of resin-bonded attachments (RBAs) before and after dynamic loading. MATERIALS AND METHODS:Forty-eight caries-free human premolars were prepared for RBAs fabricated either from a CoCr al...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2544-5
更新日期:2019-03-01 00:00:00
abstract::This study evaluated the colour parameters of composite and ceramic shade guides determined using a colorimeter and digital imaging method with illuminants at different colour temperatures. Two different resin composite shade guides, namely Charisma (Heraeus Kulzer) and Premise (Kerr Corporation), and two different ce...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0329-6
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:To explore whether a computed tomography (CT) examination of the head and neck region induces biological damage and whether the damage was correlated with the radiation dose. MATERIALS AND METHODS:Peripheral blood was taken from 33 individuals who received head and neck CT examinations. Blood samples were d...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03331-3
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was to determine the prevalence of permanent first molar (P1M) extraction among Turkish adolescents and young adult subpopulation, and to investigate the effects of P1M extraction on development of the third molars (3Ms) in the same quadrant. MATERIALS AND METHODS:A retrospectiv...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1121-1
更新日期:2014-01-01 00:00:00
abstract::This study compared shear bond strengths of six self-adhesive resin cements to zirconium oxide ceramic with and without air-particle abrasion. One hundred twenty zirconia samples were air-abraded (group SB; n = 60) or left untreated (group NO). Composite cylinders were bonded to the zirconia samples with either BisCem...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0278-0
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVES:Evaluate the bone remodeling during orthodontic movement with corticotomy when submitted to low-intensity electrical stimulation application (microcurrent-MC) and low-level laser therapy (LLLT). MATERIAL AND METHODS:One hundred and fifty Wistar rats were divided into the following 5 groups: (C) submitted to...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02845-9
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of fluoride dentifrices combined with sodium hexametaphosphate (HMP) on enamel demineralization in vitro. MATERIAL AND METHODS:Enamel bovine blocks were selected by initial surface hardness (SHi) and then divided into five experimental groups (n = 12): placebo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1707-x
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVES:The purpose of this systematic review was to examine if, in adult patients, the absence or presence of smoking influenced the prevalence of periapical periodontitis (PP). MATERIALS AND METHODS:Databases were searched, and original research manuscripts up to June 2019 were identified by two reviewers. The Ne...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03094-6
更新日期:2020-02-01 00:00:00
abstract::Bcl-2 is a family of proteins involved in protecting the cell against death stimuli or in promoting cell death. The aim of the present study was to determine the effect of nifedipine treatment on the expression of bcl-2 protein in rat gingival tissues. Rats were given gastric intubation with various concentrations and...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-003-0194-7
更新日期:2003-03-01 00:00:00
abstract:OBJECTIVES:This study aims to test the influence of different surface treatments and conditioning on the shear bond strength between polyetherketone (PEEK) and composite. MATERIALS AND METHODS:Surfaces (570 plates) were used untreated, etched, air-particle abraded or activated with silica-modified alumina oxide. Surfa...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1294-2
更新日期:2015-04-01 00:00:00