Abstract:
OBJECTIVES:Targeted modifications of the bulk implant surfaces using bioactive agents provide a promising tool for improvement of the long-term bony and soft tissue integration of dental implants. In this study, we assessed the cellular responses of primary human gingival fibroblasts (HGF) to different surface modifications of titanium (Ti) and titanium nitride (TiN) alloys with type I collagen or cyclic-RGDfK-peptide in order to define a modification improving long-term implants in dental medicine. MATERIALS AND METHODS:Employing Ti and TiN implants, we compared the performance of simple dip coating and anodic immobilization of type I collagen that provided collagen layers of two different thicknesses. HGF were seeded on the different coated implants, and adhesion, proliferation, and gene expression were analyzed. RESULTS:Although there were no strong differences in initial cell adhesion between the groups at 2 and 4 hours, we found that all surface modifications induced higher proliferation rates as compared to the unmodified controls. Consistently, gene expression levels of cell adhesion markers (focal adhesion kinase (FAK), integrin beta1, and vinculin), cell differentiation markers (FGFR1, TGFb-R1), extracellular protein markers (type I collagen, vimentin), and cytoskeletal protein marker aktinin-1 were consistently higher in all surface modification groups at two different time points of investigation as compared to the unmodified controls. CONCLUSION:Our results indicate that simple dip coating of Ti and TiN with collagen is sufficient to induce in vitro cellular responses that are comparable to those of more reliable coating methods like anodic adsorption, chemical cross-linking, or RGD coating. TiN alloys do not possess any positive or adverse effects on HGF. CLINICAL RELEVANCE:Our results demonstrate a simple, yet effective, method for collagen coating on titanium implants to improve the long term integration and stability of dental implants.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Ritz U,Nusselt T,Sewing A,Ziebart T,Kaufmann K,Baranowski A,Rommens PM,Hofmann Adoi
10.1007/s00784-016-1784-5subject
Has Abstractpub_date
2017-01-01 00:00:00pages
255-265issue
1eissn
1432-6981issn
1436-3771pii
10.1007/s00784-016-1784-5journal_volume
21pub_type
杂志文章abstract:OBJECTIVES:This study was established to investigate whether the chemokines CXCL1, CCL2, and CCL5 are produced in periodontal cells and tissues and, if so, whether their levels are regulated by microbial and/or mechanical signals. MATERIALS AND METHODS:The chemokine expression and protein levels in gingival biopsies f...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03244-1
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Cleft lip and palate (CLP) represents the most common malformation of the midfacial region worldwide. It can be suggested that the facial stigmatization, the speech impediment, and the long-standing pressure of treatment cause a range of life stressors. Neurocortical information is influenced by physiologica...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1465-9
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:To compare, by Micro-CT and confocal laser scanning microscopy (CLSM), the ability of the Wave One Gold and Wave One systems to remove filling material from mesial canals of mandibular molars, effective time spent; quantity of extruded material, and percentage of sealer in the dentinal tubules after retrea...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2441-y
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the effects of periodontal treatment on the inflammatory markers in gingival crevicular fluid and the concentration of salivary cortisol between non-pregnant and pregnant women with gingivitis. MATERIALS AND METHODS:This study included 30 non-pregnant women (mean age 27....
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2569-9
更新日期:2018-07-31 00:00:00
abstract::Within the context of preventing non-communicable diseases, the World Health Report (2002) and the WHO Global Oral Health Program (2003) put forward a new strategy of disease prevention and health promotion. Greater emphasis is placed on developing global policies in oral health promotion and oral disease prevention. ...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-007-0178-0
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVES:The main goal of the present study was to compare the biomechanical stability of locking plates and conventional miniplate combinations in human mandibles reconstructed with fibular grafts. MATERIALS AND METHODS:A specially developed and well-proven testing device reproduced the in vivo loading conditions o...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1105-1
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVES:To evaluate the prevalence, clinical features, and risk factors of dentin hypersensitivity (DH) in a Brazilian population. MATERIALS AND METHODS:300 patients at the Dentistry Clinic of the University of São Paulo participated in this study. The subjects completed a questionnaire regarding their personal inf...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1008-1
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Our aim was to assess the anti-biofilm ability of previously unverified individual D-amino acids (DAAs), to produce plasma polymer encapsulated DAAs (PPEDAAs), to measure the shell thickness and subsequent release of DAAs, and to assess the effects of PPEDAAs on Enterococcus faecalis biofilms. MATERIALS AND ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03663-0
更新日期:2020-11-02 00:00:00
abstract:OBJECTIVE:SLC22A4/5 single nucleotide polymorphisms (SNPs) have been reported to affect inflammatory diseases. We report the relationship of these polymorphisms with adiposity and tooth loss as elucidated in a 10-year follow-up study. METHODS:Participants of the Study of Health in Pomerania (SHIP, N = 4105) were genot...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03594-w
更新日期:2021-02-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to introduce a novel three-dimensional (3D) method to quantify the relative amount of different tissue components in bone substitute-treated defects by means of integration of confocal laser imaging into micro-computed tomography (μCT) analysis. MATERIALS AND METHODS:One standardize...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1081-5
更新日期:2014-05-01 00:00:00
abstract::The objective of this study is to evaluate flexural strength, flexural modulus, compressive strength, curing temperature, curing depth, volumetric shrinkage, water sorption, and hygroscopic expansion of two self-, three dual-, and three light-curing resin-based core materials. Flexural strength and water sorption were...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-010-0405-y
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the immunohistochemical expressions of PD1, CD4+, and CD8+ in premalignant lesions (OPML) that were transformed into oral squamous cell carcinoma OSCC (OPML-OSCC), in OSCC and also in premalignant lesions that were not transformed into OSCC (OPML-NOSSC). MATERIALS AND ME...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2622-8
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVES:Facial asymmetries in oculoauriculovertebral spectrum (OAVS) patients might require surgical corrections that are mostly based on qualitative approach and surgeon's experience. The present study aimed to develop a quantitative 3D CT imaging-based procedure suitable for maxillo-facial surgery planning in OAVS...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-015-1660-8
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was to investigate different fluorescence-based, two-color viability assays for visualization and quantification of initial bacterial adherence and to establish reliable alternatives to the ethidium bromide staining procedure. MATERIALS AND METHODS:Bacterial colonization was att...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0792-3
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVES:Numerous different attachments are used to retain overdentures on implants. The purpose of this study was to evaluate a new chairside attachment system based on polyvinylsiloxane (PVS). MATERIAL AND METHODS:A total of 250 specimens were fabricated (n = 10) to measure the retention force (RF) in dependence o...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1307-1
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVES:This in vitro study evaluated the effect of fiber insertion depth on antimicrobial efficacy of antimicrobial photodynamic therapy (aPDT) using a photosensitizer (PS; toluidine blue) and a red light-emitting diode (LED) in root canals infected with Enterococcus faecalis. MATERIALS AND METHODS:Single-rooted e...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1948-3
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:This systematic review (SR) aimed to summarise and critically appraise available evidence about the association between the synergistic consumption of alcohol and tobacco and the occurrence of oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS:Observational studies investigating the association betw...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-019-02958-1
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:This study aimed to conduct a methodological assessment of paper-based systematic reviews (SR) published in oral health using a validated checklist. A secondary objective was to explore temporal trends on methodological quality. MATERIAL AND METHODS:Two electronic databases (OVID Medline and OVID EMBASE) we...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-015-1663-5
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the effects of a hemostatic collagen sponge and a collagen sponge sealed with a bio-adhesive material on the palatal donor sites with the purpose of minimizing postoperative pain after epithelialized gingival graft (EGG) harvesting. MATERIAL AND METHODS:The present study...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2455-5
更新日期:2019-01-01 00:00:00
abstract::The aim of this study was to evaluate the clinical performance of S(3) Bond (Kuraray Corp., Japan) and G-Bond (GC Corp., Japan) all-in-one bonding agents, over 3 years in non-carious cervical lesions (NCCLs). Ethics Committee approval was obtained, and 60 restorations were placed in 11 patients aged 45-84 years (mean ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-011-0595-y
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVES:This study was designed to investigate the antimicrobial properties, compressive strength and fluoride release capacities of high-viscous glass ionomer cements (GICs) after incorporation of cinnamon and thyme essential oils. MATERIALS AND METHODS:Experimental-modified GICs were prepared by incorporation of ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03493-0
更新日期:2020-08-15 00:00:00
abstract::Oral verrucous carcinoma (OVC) is a rare variant of squamous cell carcinoma with a characteristic morphology and specific behavior. To date, few studies are available focusing the prevalence and clinicopathologic features of the oral verrucous carcinoma in Brazilian population. A total of 3,500 primary oral well-diffe...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-006-0050-7
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVES:The main purpose of this study was to investigate the detailed healing process of the roots and surrounding periodontium [cementum, periodontal ligament (PDL), and bone] at different time points after intentional root damage with miniscrew implants (MSIs). MATERIALS AND METHODS:After cone-beam computed tomo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2194-z
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:This case series compared the clinical survival of tooth-implant-supported (TI-S) and tooth-supported (T-S) three-unit fixed dental prostheses (FDPs) with zirconia frameworks and described the incidence of biological and technical complications. MATERIALS AND METHODS:Forty-four patients received 27 TI-S FDP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1594-1
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVES:Several studies have shown the possible involvement of Helicobacter pylori infection in individuals with recurrent aphthous stomatitis (RAS), but the relationship remains controversial. This meta-analysis was performed to validate the association between RAS and H. pylori infection. MATERIALS AND METHODS:Th...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00784-014-1230-5
更新日期:2014-07-01 00:00:00
abstract:OBJECTIVE:The study aimed to evaluate the effect of internal silver coating as a countermeasure to crestal bone loss around implants with or without multiple abutment disconnections/reconnections. MATERIALS AND METHODS:Following tooth extraction, 48 implants with connected healing abutments (24 implants internally coa...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03416-z
更新日期:2021-02-01 00:00:00
abstract::The alveolar cleft in patients with clefts of lip, alveolus and palate (CLAP) is usually reconstructed with an autologous bone graft. Harvesting of autologous bone grafts is associated with more or less donor site morbidity. Donor site morbidity could be eliminated if bone is fabricated by growth factor-aided tissue e...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-011-0547-6
更新日期:2011-06-01 00:00:00
abstract::The aims of the present study were: a) to assess the impact of the intraoral location on the rate of biofilm growth, and b) to establish an in vivo biofilm model to examine intraoral biofilm growth. Eight healthy volunteers wore acrylic splints with 15 glass slabs each in the upper and lower jaws to build up plaque. A...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-004-0255-6
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVES:This study aims to compare the clinical outcomes of a combination of enamel matrix derivatives (EMD) and a synthetic bone graft (biphasic calcium phosphate) with EMD alone in wide and deep one- and two-wall intrabony defects 36 months after treatment. MATERIAL AND METHODS:Thirty patients with chronic period...
journal_title:Clinical oral investigations
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00784-015-1522-4
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVES:To assess the quality of clinical practice guidelines (CPGs) on screening and diagnosis of oral cancer and to describe the characteristics of their recommendations. MATERIALS AND METHODS:We systematically searched EMBASE, MEDLINE, CPG' websites, and dentistry and oncology scientific societies to identify CP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2668-7
更新日期:2019-05-01 00:00:00