Abstract:
OBJECTIVES:The aim of the study was to introduce a novel three-dimensional (3D) method to quantify the relative amount of different tissue components in bone substitute-treated defects by means of integration of confocal laser imaging into micro-computed tomography (μCT) analysis. MATERIALS AND METHODS:One standardized semisaddle intraosseous defect was prepared in the mandibles of six minipigs and scanned by an optical scanner to capture the surface of the fresh defect in a 3D manner. Subsequently, all the defects were filled with a biphasic calcium phosphate material. The animals were divided into two groups of three animals each, which were allowed to heal for 3 and 8 weeks, respectively. μCT analysis followed the two healing periods and was performed on all defect locations. The data from optical scanning and μCT were used for three-dimensional evaluation of bone formation, nonmineralized tissue ratio, and graft degradation. The integration of confocal laser scanning into μCT analysis through a superimposition imaging procedure was conducted using the software Amira (Mercury Computer Systems, Chelmsford, MA, USA). RESULTS:The feasibility of combining the confocal imaging into μCT data with regard to obtaining accurate 3D quantification was demonstrated. The amount of tissue components was identified and quantified in all the investigated samples. Quantitative analysis demonstrated that a significant increase in the amount of bone filling the defect was observed in vivo (p < 0.02) while a significant decrease in the amount of nonmineralized tissue occurred (p < 0.04). No difference in the amount of residual grafting material was detected between 3 and 8 weeks in vivo (p > 0.38). CONCLUSIONS:The combination of confocal imaging and micro-computed tomography techniques allows for analysis of different tissue types over time in vivo. This method has revealed to be a feasible alternative to current bone regeneration quantification methods. CLINICAL RELEVANCE:Assessment of bone formation in a large animal model is a key step in assessing the performance of new bone substitute materials. Reliable and accurate methods are needed for the analysis of the regenerative potential of new materials.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Clozza E,Obrecht M,Dard M,Coelho PG,Dahlin C,Engebretson SPdoi
10.1007/s00784-013-1081-5subject
Has Abstractpub_date
2014-05-01 00:00:00pages
1245-1250issue
4eissn
1432-6981issn
1436-3771pii
10.1007/s00784-013-1081-5journal_volume
18pub_type
杂志文章abstract:OBJECTIVES:The present study evaluated the effect of an enamel matrix derivative (EMD) and platelet-rich fibrin (PRF)-modified porcine-derived collagen matrix (PDCM) on human umbilical vein endothelial cells (HUVEC) in vitro. MATERIALS AND METHODS:PDCM (mucoderm®) was prepared to 6 mm (±0.1 mm) diameter discs. PDCM sa...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2170-7
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the efficacy of four different side-to-side toothbrushes and the impact of various brushing parameters on noncontact biofilm removal in an adjustable interdental space model. MATERIALS AND METHODS:A three-species biofilm, consisting of Porphyromonas gingivalis, Fu...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1969-y
更新日期:2017-06-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050038
更新日期:1998-03-01 00:00:00
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journal_title:Clinical oral investigations
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更新日期:2020-10-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-011-0642-8
更新日期:2012-10-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1050-z
更新日期:2014-04-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0329-6
更新日期:2010-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2393-2
更新日期:2018-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03616-7
更新日期:2020-10-13 00:00:00
abstract::Squamous cell carcinoma (SCC) of the oral cavity is an extremely invasive tumour of stratified squamous epithelium that spreads throughout degradation of the basement membrane (BM) and extra-cellular matrix. Oral verrucous carcinoma (VC) is a rare low-grade variant of oral SCC that penetrates into the subepithelial co...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0296-y
更新日期:2010-06-01 00:00:00
abstract::From a preventive point of view collection of data concerning carious and non-carious cervical tooth defects is definitely important. Consequently, the prevalence and distribution of different root lesions were studied and correlated with behavioral and biological factors in 50- to 60-year-old German individuals (n = ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050097
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to validate a standardized pragmatic approach to manage new oral anticoagulants (NOACs) in patients who undergo dental extractions. MATERIALS AND METHODS:This prospective case-control study in patients undergoing dental extraction included 26 patients (mean age 76 years, 57% male) ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-2010-1
更新日期:2017-09-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-007-0124-1
更新日期:2007-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1514-4
更新日期:2016-03-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0314-0
更新日期:2010-08-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03459-2
更新日期:2020-07-21 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1166-1
更新日期:2014-11-01 00:00:00
abstract::A total of 110 patients with neurofibromatosis 1 (NF1) were evaluated for their dental health. Appropriate cohorts from national Finnish databases were used as reference. The results showed that NF1 patients presented lower rate of caries compared to controls in age groups under 35 years. The differences between NF1 p...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0341-x
更新日期:2011-02-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2019-02-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-2013-y
更新日期:2017-09-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03497-w
更新日期:2020-08-12 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03706-6
更新日期:2020-12-03 00:00:00
abstract:OBJECTIVES:This study aims to investigate the additional influence of multiple applications of antimicrobial photodynamic therapy (aPDT) in smokers with chronic periodontitis. MATERIALS AND METHODS:Twenty smokers with chronic periodontitis were treated in a split-mouth design study with aPDT adjunct to Scaling and Roo...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2740-3
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:This study evaluated the effect of the administration of pre-operative dexamethasone on tooth sensitivity stemming from in-office bleaching. MATERIALS AND METHODS:A triple-blind, parallel-design, randomized clinical trial was conducted on 70 volunteers who received dexamethasone or placebo capsules. The dru...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2452-8
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the effect of different seating forces during cementation in cement-ceramic microtensile bond strength (μTBS). MATERIALS AND METHODS:Forty-five blocks (5 × 5 × 4 mm(3)) of a glass-infiltrated alumina-based ceramic (In-Ceram Alumina) were fabricated according to the manu...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-011-0668-y
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To describe and explore the association between proxy-reported sleep bruxism (SB) and quality of life (QoL) in a population of Colombian children belonging to different social layers. METHODS:The parents of 1556, 6-13-year-old school children, were administered the Pediatric Inventory of Quality of Life (Ped...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1901-5
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVES:Mandibular reconstruction after segmental mandibulectomy can be challenging without virtual surgical planning and osteotomy guides. The purpose of this study was to analyze anatomic parameters to facilitate the evaluation of ideal fibula wedge osteotomies to reconstruct the neomandibula in a simple and cost-...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03177-4
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the palatal total support tissues (TSTs) and bone support tissues (BSTs) at 5-mm paramedian section to the midsagittal suture between mouth breathers with high-narrow palates and nose breathers with normal palates and confirm the practicability and limitation on superimpo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03004-w
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:This study aims to evaluate the effect of a minimally invasive mesial-occlusal-distal (mod) preparation on the marginal adaptation of ceramic and composite inlays with the aim of saving sound dental substance. MATERIALS AND METHODS:Class II mod cavities were prepared in 50 extracted human molars and randoml...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0988-1
更新日期:2014-01-01 00:00:00