Abstract:
OBJECTIVES:The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP). MATERIALS AND METHODS:We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxillofacial surgery and then analyzed their cell surface antigens by flow cytometry. Then, the cells were cultured in osteogenic medium for 14 days. Measurement of bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), and calcium deposition and alizarin red staining were performed to evaluate the osteoblastic differentiation ability of both cell types. RESULTS:ASCs and DFAT cells were positive for CD90 and CD105 and negative for CD11b, CD34, and CD45. BAP (days 3 and 7), OCN (day 14), and calcium deposition (days 7 and 14) within DFAT cell cultures were significantly higher than those in ASC cultures. The alizarin red-stained area in DFAT cell cultures, which indicates mineralized matrix deposition, was stained more strongly than that in ASC cultures. CONCLUSIONS:The cell surface antigens of ASCs and DFAT cells tend to be similar. Furthermore, the osteoblastic differentiation ability of human DFAT cells is higher than that of ASCs from the BFP. CLINICAL RELEVANCE:Isolation of DFAT cells from the BFP has an esthetic advantage because the BFP can be obtained via the oral cavity without injury to the external body surface. Therefore, we consider that DFAT cells from the BFP are an ideal cell source for bone tissue engineering.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Kishimoto N,Momota Y,Hashimoto Y,Tatsumi S,Ando K,Omasa T,Kotani Jdoi
10.1007/s00784-013-1166-1subject
Has Abstractpub_date
2014-11-01 00:00:00pages
1893-901issue
8eissn
1432-6981issn
1436-3771journal_volume
18pub_type
杂志文章abstract:OBJECTIVE:Noninvasive optical methods such as photoplethysmography, established for blood pulse detection in organs, have been proposed for vitality testing of human dental pulp. However, no information is available on the mechanism of action in a closed pulp chamber and on the impairing influence of other than pulpal ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1115-z
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:The present case-control study aims to assess the occurrence of alexithymic traits in burning mouth syndrome (BMS) subjects and to correlate alexithymic traits to anxious and depressive traits in BMS subjects. MATERIALS AND METHODS:Prospectively enrolled BMS and control subjects were administered the 20-ite...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1416-5
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the immunohistochemical expressions of PD1, CD4+, and CD8+ in premalignant lesions (OPML) that were transformed into oral squamous cell carcinoma OSCC (OPML-OSCC), in OSCC and also in premalignant lesions that were not transformed into OSCC (OPML-NOSSC). MATERIALS AND ME...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2622-8
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVES:Τhis study aims at determining the ability of cone beam computed tomography (CBCT) to visualize critical-size defects (CSD) created at rat calvaria and filled with 75/25 w/w nano-hydroxyapatite/chitosan (nHAp/CS) scaffolds, prior to their histological investigation. MATERIALS AND METHODS:Thirty adult Spragu...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02939-4
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Targeted modifications of the bulk implant surfaces using bioactive agents provide a promising tool for improvement of the long-term bony and soft tissue integration of dental implants. In this study, we assessed the cellular responses of primary human gingival fibroblasts (HGF) to different surface modifica...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1784-5
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVES:The purpose of this systematic review was to examine if, in adult patients, the absence or presence of smoking influenced the prevalence of periapical periodontitis (PP). MATERIALS AND METHODS:Databases were searched, and original research manuscripts up to June 2019 were identified by two reviewers. The Ne...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03094-6
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:Clinicians agree that children with isolated cleft lip have fewer cleft-associated problems than children with cleft lip and palate. Unfortunately, for isolated cleft lip children, the risk of cleft-associated problems is unknown and maybe underestimated. Often, these children do not get the required follow-...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03367-5
更新日期:2020-06-04 00:00:00
abstract::The translucency of teeth allows the non-destructive subsurface visualisation of their microstructure by confocal laser scanning microscopy (CLSM) at a level of about 150 microns below the surface. The dentine-enamel junction (DEJ) is accessible only directly adjacent to the cervix of the tooth. Therefore teeth have t...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050038
更新日期:1998-03-01 00:00:00
abstract::The objective of this prospective clinical follow-up was to evaluate the 2-year clinical performance of a nanofilled resin composite in class II restorations. The restorations were made with and without intermediary layer of a nanofilled flowable resin composite studied in an intraindividual comparison. Each participa...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-010-0485-8
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Long-term culture system is used to prevent the impediment of insufficient cells and is good for low starting materials such as dental pulp or periodontal ligament. In general, although cell viability and functionality are the most common aspects taken into consideration in culturing cells for...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1497-1
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate through a systematic review the extraction of third molars as a risk factor for temporomandibular disorders (TMDs). TYPES OF STUDIES REVIEWED:Randomized and nonrandomized controlled clinical trials where patients underwent third molar extraction and with qualitative eva...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-020-03277-6
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:Genome-wide association studies (GWAS) and literature have identified polymorphisms in the KCNJ11, HNF1A, IRS1, TCF7L2, CDKAL1, CDKN2B, RPSAP52, GPR45 HHEX, IL18, and RUNX2 genes associated with type 2 diabetes mellitus (T2DM) and/or periodontitis (P) in diverse populations, and we sought to evaluate them as ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03717-3
更新日期:2021-01-03 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the palatal total support tissues (TSTs) and bone support tissues (BSTs) at 5-mm paramedian section to the midsagittal suture between mouth breathers with high-narrow palates and nose breathers with normal palates and confirm the practicability and limitation on superimpo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03004-w
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:Our aim was to assess the anti-biofilm ability of previously unverified individual D-amino acids (DAAs), to produce plasma polymer encapsulated DAAs (PPEDAAs), to measure the shell thickness and subsequent release of DAAs, and to assess the effects of PPEDAAs on Enterococcus faecalis biofilms. MATERIALS AND ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03663-0
更新日期:2020-11-02 00:00:00
abstract:OBJECTIVES:Resin-based composites may leach monomers such as triethylene-glycol dimethacrylate (TEGDMA), which could contribute to intrapulpal inflammation. The aim of this investigation was to examine whether various concentrations of TEGDMA are able to influence dentally relevant Matrix metalloproteinase (MMP)-2, MMP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03545-5
更新日期:2020-08-26 00:00:00
abstract:OBJECTIVES:This in vitro study evaluated the effect of fiber insertion depth on antimicrobial efficacy of antimicrobial photodynamic therapy (aPDT) using a photosensitizer (PS; toluidine blue) and a red light-emitting diode (LED) in root canals infected with Enterococcus faecalis. MATERIALS AND METHODS:Single-rooted e...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1948-3
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:Ankaferd BloodStopper® (ABS) is an herbal extract which has been used historically as a hemostatic agent in traditional Turkish medicine. ABS comprises of standardized mixture of herbs Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. In addition to its hemostatic e...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2399-9
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:Previous research revealed that autogenous tooth roots may be biologically equivalent to conventional bone grafts for lateral ridge augmentation. However, these analyses were limited to two dimensions, whereas healing is a volumetric process. The present study aimed at volumetrically assessing the microstruc...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2723-4
更新日期:2019-07-01 00:00:00
abstract::This study deals with 4971 implants (Brånemark system) installed in 1315 patients, either fully or partially edentulous, and followed from implant installation up to the last control. A predominance of female patients (61%) and a nearly equal number of upper and lower jaws characterised the study group. Patients were ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050107
更新日期:2000-03-01 00:00:00
abstract:OBJECTIVES:To determine the detectability of the intra-oral photographic method in comparison to the baseline, comprehensive dental examination (CDE), in children when performed by different levels of dental practitioners. MATERIALS AND METHODS:As part of a quality assurance program, intra-oral photographs were obtain...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2700-y
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:For well-defined deep (> 2/3 dentin extension) carious lesions, selective (SE) or stepwise (SW) carious tissue removals have been recommended, while there is limited comparative evidence for both. We compared SE and SW over 24 months in a randomized controlled trial. METHODS:A two-arm superiority trial was ...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-020-03536-6
更新日期:2021-02-01 00:00:00
abstract::Complex human tissues harbour stem cells and/or precursor cells, which are responsible for tissue development or repair. Recently, dental tissues such as periodontal ligament (PDL), dental papilla or dental follicle have been identified as easily accessible sources of undifferentiated cells. The dental stem cell biolo...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-007-0170-8
更新日期:2008-06-01 00:00:00
abstract::Fluoride is used in dentistry as a prophylactic agent to reduce caries rates due to the demineralization/remineralization effect and its influence on the metabolism of cariogenic bacteria. The purpose of this study was to evaluate the cytotoxic effects of sodium fluoride (NaF) on three different cell lines and the ant...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-002-0157-4
更新日期:2002-06-01 00:00:00
abstract:OBJECTIVES:Detecting bacterial activity is considered a promising approach to monitor shifts from symbiosis to dysbiosis in oral microbiome. The present study aimed at investigating both the relative bacterial activity and the lactate dehydrogenase (ldh) gene expression of caries-associated bacteria in a site-specific ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03691-w
更新日期:2020-11-23 00:00:00
abstract:OBJECTIVES:C-shaped root canal configuration is an unusual root canal morphology and one of the most often anatomical variations in root canal systems of second mandibular molars. The purpose of this study was to assess the prevalence, symmetry and configuration of C-shaped canals in first and second mandibular molars ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1993-y
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVES:To assess the association between periodontal disease and Helicobacter pylori (H. pylori) infection in oral cavity. MATERIALS AND METHODS:We searched PubMed, Embase, Web of Science, Cochrane library, Gray literature, and clinicaltrials.gov for eligible studies up to September 25, 2019. Odds ratios (ORs) and...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-020-03330-4
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:To evaluate the marginal and internal fit of CAD/CAM-generated frameworks for 4-unit, fixed dental prostheses (FDPs) from zirconia (Z) and cobalt-chromium alloy (C) made with conventional (CI) and digital impressions (DI). MATERIALS AND METHODS:A titanium model was digitized with an intraoral scanner (DI, L...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1513-5
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVES:Nitrogen-containing bisphosphonates induce osteonecrosis mostly in the jaw and less frequently in other bones. Because of the crucial role of periosteal perfusion in bone repair, we investigated zoledronate-induced microcirculatory reactions in the mandibular periosteum in comparison with those in the tibia ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1347-6
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVES:Ventilator-associated pneumonia (VAP) is the most frequent hospital-acquired infections in intensive care units (ICU). In the bundle of care to prevent the VAP, the oral care is very important strategies, to decrease the oropharyngeal bacterial colonization and presence of causative bacteria of VAP. In view ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2289-6
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:Bisphosphonate-associated osteonecrosis of the jaw is a severe side effect in patients receiving nitrogen-containing bisphosphonates (N-BPs). One characteristic is its high recurrence rate; therefore, basic research for new therapeutic options is necessary. N-BPs inhibit the farnesylpyrophosphate synthase in...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1320-4
更新日期:2015-06-01 00:00:00