Abstract:
OBJECTIVES:Detecting bacterial activity is considered a promising approach to monitor shifts from symbiosis to dysbiosis in oral microbiome. The present study aimed at investigating both the relative bacterial activity and the lactate dehydrogenase (ldh) gene expression of caries-associated bacteria in a site-specific natural biofilm. MATERIAL AND METHODS:Sixty subjects (age, mean ± SE: 30.1 ± 1.4) were allocated to two groups: caries-free subjects (CF) or caries-active subjects (CA). CF presented one sound surface (CFS, n = 30). CA presented two donor sites: a cavitated caries lesion (CAC, n = 30) and a sound reference surface (CAS, n = 30). Real-time quantitative PCR (q-PCR) on species or genus level and total bacteria was performed targeting the 16S gene, the 16S rRNA, the ldh gene, and the ldh mRNA (increasing 16S ribosomal RNA copy numbers can function as an indicator of increased energy metabolism). As the 16S rRNA abundance represents the number of ribosomes, while the 16S gene abundance represents the number of genomes, the quotient of the relative abundances functions as a measure for the relative bacterial activity (%). RESULTS:Both lactobacilli and S. mutans showed the highest relative bacterial activity in CAC ((mean ± SE) 218 ± 60% and 61 ± 16%, respectively) and the lowest values for both sound reference surfaces (69 ± 48%; 8 ± 3%). Significant differences were found between CAC and CAS as well as between CAC and CFS for both lactobacilli and S. mutans (p < 0.05). The ldh gene expression of lactobacilli and S. mutans only showed moderate values in CAC (1.90E+03 ± 2.11E+03; 2.08E+04 ± 4.44E+04 transcripts/μl) and CFS (2.04E+03 ± 2.74E+03; 8.16E+03 ± 6.64E+03 transcripts/μl); consequently no significant differences were detected. CONCLUSION AND CLINICAL RELEVANCE:Caries-associated bacteria (lactobacilli and S. mutans) showed the highest relative bacterial activity in plaque of cavitated lesions, the lowest in sound surfaces, allowing the detection of a significant activity shift in health and disease for caries-active patients. However, no significant differences in ldh gene expression could be determined.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Walther C,Zumbülte S,Faerber CM,Wierichs RJ,Meyer-Lueckel H,Conrads G,Henne K,Esteves-Oliveira Mdoi
10.1007/s00784-020-03691-wsubject
Has Abstractpub_date
2020-11-23 00:00:00eissn
1432-6981issn
1436-3771pii
10.1007/s00784-020-03691-wpub_type
杂志文章abstract:OBJECTIVES:The clinical standard for alveolar cleft osteoplasty is augmentation with autologous bone being available in limited amounts and might be associated with donor site morbidity. The aim of the present study was the creation of tissue-engineered bone grafts and their in vivo evaluation regarding their potential...
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abstract::The goal of this investigation was to evaluate the effect of postoperative exposure of two different bioresorbable membranes on the guided tissue regeneration (GTR) healing results compared to nonexposed sites. In each of 25 patients one pair of contralateral intrabony lesions was treated either with polylactic acid (...
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更新日期:2016-12-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-020-03365-7
更新日期:2021-01-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,随机对照试验
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更新日期:2009-12-01 00:00:00
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journal_title:Clinical oral investigations
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更新日期:2016-03-01 00:00:00
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更新日期:2017-03-01 00:00:00
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更新日期:2017-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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abstract::The main objective of this study was to identify the risk factors for the incidence of visible caries experience in a cohort of preschool children living in Flanders. Data were collected from 1,057 children; validated questionnaires on oral health-related behaviour were completed by parents at birth (2003-2004), at ag...
journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00