当前位置： SCI文献检索 > Clinical Oral Investigations期刊下所有文献 > Expression patterns of immune genes in long-term cultured dental stem cells.

Expression patterns of immune genes in long-term cultured dental stem cells.

Abstract：

BACKGROUND AND OBJECTIVES:Long-term culture system is used to prevent the impediment of insufficient cells and is good for low starting materials such as dental pulp or periodontal ligament. In general, although cell viability and functionality are the most common aspects taken into consideration in culturing cells for a long term, they may not truly represent the biological state of the cells. Hence, we explored the behaviour of another important aspect which is the immune properties in long-term cultured cells. METHODS:Dental pulp stem cells from deciduous (SHED; n = 3) and permanent (DPSCs; n = 3) teeth as well as periodontal ligament stem cells (PDLSCs; n = 3) were cultured under identical culture condition. The immune properties of each cell lines were profiled at passage 2 [P2] and passage 9 [P9] as early and late passages, respectively. This was further validated at the protein level using the Luminex platform. RESULTS:A major shift of genes was noticed at P9 with SHED being the highest. SHED cultured at P9 displayed many genes representing pathogen recognition (P < 0.001), immune signalling (P < 0.001, pro-inflammatory (P < 0.001), anti-inflammatory (P < 0.001) and immune-related growth and stimulation factor (P < 0.001) as compared to DPSCs and PDLSCs. Surprisingly, SHED also expressed many cytotoxicity genes (P < 0.001). CONCLUSIONS:Communally, instabilities of immune genes from our findings suggest that long-term cultured cells may not be feasible for transplantation purposes. CLINICAL RELEVANCE:A complete biological characterization covering all major aspects including immune properties should be made as prerequisite criteria prior to the use of long-term cultured stem cells in clinical settings.

journal_name

Clin Oral Investig

journal_title

Clinical oral investigations

authors

Jayaraman P,Govindasamy V,Gnanasegaran N,Kunasekaran W,Vasanthan P,Musa S,Kasim NH

doi

10.1007/s00784-015-1497-1

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

109-16

issue

1

eissn

1432-6981

issn

1436-3771

pii

10.1007/s00784-015-1497-1

journal_volume

20

pub_type

杂志文章

相关文献

Clinical Oral Investigations文献大全
  • A new method for chlorhexidine (CHX) determination: CHX release after application of differently concentrated CHX-containing preparations on artificial fissures.

    abstract：:Aims of the study were (1) to establish a method for quantification of chlorhexidine (CHX) in small volumes and (2) to determine CHX release from differently concentrated CHX-containing preparations, varnishes, and a CHX gel applied on artificial fissures. CHX determination was conducted in a microplate reader using p...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-007-0166-4

    authors： Attin T,Abouassi T,Becker K,Wiegand A,Roos M,Attin R

    更新日期：2008-09-01 00:00:00

  • Effects of cachaça, a typical Brazilian alcoholic beverage, on submandibular glands of rats: a histomorphometric and biochemical study.

    abstract：OBJECTIVE:This study aimed to evaluate the effects of chronic consumption of cachaça, a Brazilian beverage containing alcohol, on submandibular glands (SM) of rats by using histomorphometric and biochemical parameters. MATERIALS AND METHODS:Twenty-four male rats (40 days of age) were assigned into the following four g...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-020-03290-9

    authors： Dal Prá KJ,de Fatima Moraes da Silva A,Chaves-Neto AH,Soubhia AMP

    更新日期：2020-12-01 00:00:00

  • Assessment of craniofacial maturation in preadolescents with cleft lip and/or palate using the cervical vertebral maturation method.

    abstract：OBJECTIVES:Aberrant growth of the maxillomandibular complex in patients with cleft lip and/or palate (CL/P) can be managed with dentofacial orthopaedics. However, no consensus has been reached regarding timing and evolution of the maturational stages. Therefore, the aim of this study is to determine if patients with CL...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-021-03790-2

    authors： Thierens LAM,Manalili L,De Roo N,Verdonck A,De Llano-Pérula MC,De Pauw GAM

    更新日期：2021-01-23 00:00:00

  • Psychological symptoms and self-image of patients with complaints attributed to dental restorative materials.

    abstract：OBJECTIVES:The aim was to study self-image and the level of psychological symptoms in patients with symptoms attributed to their dental restorative materials. MATERIALS AND METHODS:A questionnaire containing questions regarding dental and medical history was answered by 257 participants, one group with local oral symp...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-018-2707-4

    authors： Mårell L,Bergdahl J,Tillberg A,Stenberg B,Berglund A

    更新日期：2019-06-01 00:00:00

  • Orthodontic cell stress modifies proinflammatory cytokine expression in human PDL cells and induces immunomodulatory effects via TLR-4 signaling in vitro.

    abstract：OBJECTIVE:Biomechanical orthodontics loading of the periodontium initiates a cascade of inflammatory signaling events that induce periodontal remodeling and finally facilitate orthodontic tooth movement. Pattern recognition receptors such as toll-like receptors (TLRs) have been well characterized for their ability to i...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-019-03111-8

    authors： Marciniak J,Lossdörfer S,Knaup I,Bastian A,Craveiro RB,Jäger A,Wolf M

    更新日期：2020-04-01 00:00:00

  • Histotomography of the odontoblast processes at the dentine-enamel junction of permanent healthy human teeth in the confocal laser scanning microscope.

    abstract：:The translucency of teeth allows the non-destructive subsurface visualisation of their microstructure by confocal laser scanning microscopy (CLSM) at a level of about 150 microns below the surface. The dentine-enamel junction (DEJ) is accessible only directly adjacent to the cervix of the tooth. Therefore teeth have t...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s007840050038

    authors： Grötz KA,Duschner H,Reichert TE,de Aguiar EG,Götz H,Wagner W

    更新日期：1998-03-01 00:00:00

  • Comparative in vivo study of alloy titanium implants with two different surfaces: biomechanical and SEM analysis.

    abstract：OBJECTIVES:The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by Al2O3 sandblasting and acid etching (SBAS). MATERIALS AND METHODS:Before surgery, topographic characterization was performed by SEM-EDX and...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-019-02872-6

    authors： Souza FÁ,Furtado TSM,Dayube URC,Melo WM,Nishioka RS,Poli PP,Maiorana C,de Carvalho PSP

    更新日期：2019-12-01 00:00:00

  • Quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time PCR.

    abstract：:The present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (PCR). Twenty patients with untreated chronic periodontitis were randomized into two groups. Before subgingival instrumentati...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00784-009-0333-x

    authors： Jervøe-Storm PM,AlAhdab H,Koltzscher M,Fimmers R,Jepsen S

    更新日期：2010-10-01 00:00:00

  • Comparison of efficiency of the retreatment procedure between Wave One Gold and Wave One systems by Micro-CT and confocal microscopy: an in vitro study.

    abstract：INTRODUCTION:To compare, by Micro-CT and confocal laser scanning microscopy (CLSM), the ability of the Wave One Gold and Wave One systems to remove filling material from mesial canals of mandibular molars, effective time spent; quantity of extruded material, and percentage of sealer in the dentinal tubules after retrea...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-018-2441-y

    authors： Canali LCF,Duque JA,Vivan RR,Bramante CM,Só MVR,Duarte MAH

    更新日期：2019-01-01 00:00:00

  • Microhardness changes in surface enamel after application of bioadhesive fluoride tablets in situ.

    abstract：:The effect of fluoride released by bioadhesive tablets was evaluated in an in situ model in human volunteers. Eight volunteers carried four to six polished human enamel samples in a lower lingual device for a period of 5 days without fluoride supplements (control) and 5 days using one bioadhesive tablet (0.5 mg F) per...

    journal_title：Clinical oral investigations

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1007/s007840000076

    authors： Bottenberg P,Cleymaet R,Röhrkasten K,Lampert F

    更新日期：2000-09-01 00:00:00

  • Effectiveness of 2% Articaine as an anesthetic agent in children: randomized controlled trial.

    abstract：AIM:The aim of this study was to compare the effectiveness of 2% articaine and 2% lignocaine in achieving adequate anesthesia in children between the age group of 6-13 years using inferior alveolar nerve block. METHODS:A triple blinded randomized controlled trial was conducted in 180 participants (90 patients- 2% arti...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00784-018-2775-5

    authors： Ramadurai N,Gurunathan D,Samuel AV,Subramanian E,Rodrigues SJL

    更新日期：2019-09-01 00:00:00

  • Connective tissue graft as a biological barrier for guided tissue regeneration in intrabony defects: a histological study in dogs.

    abstract：BACKGROUND:The use of the autogenous periosteal graft as biological barrier has been proposed for periodontal regeneration. The aim of this study was to evaluate the histometric findings of the subepithelial connective tissue graft as barrier in intrabony defects compared to a bioabsorbable membrane. METHODS:Three-wal...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-014-1323-1

    authors： Ribeiro FS,Pontes AE,Zuza EP,da Silva VC,Lia RC,Marcantonio Junior E

    更新日期：2015-06-01 00:00:00

  • Complications and survival rates of teeth after dental trauma over a 5-year period.

    abstract：OBJECTIVES:The purpose of this study was to evaluate whether the current guidelines of the International Association of Dental Traumatology (IADT) for emergency care of traumatised teeth result in lower complication rates. MATERIALS AND METHODS:Therapeutic strategies of 361 dental injuries in 291 patients treated at a...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-012-0817-y

    authors： Bücher K,Neumann C,Thiering E,Hickel R,Kühnisch J,International Association of Dental Traumatology.

    更新日期：2013-06-01 00:00:00

  • Retentive characteristics of individual and prefabricated polyvinylsiloxane overdenture attachments: alternative treatment options for geriatric patients.

    abstract：OBJECTIVES:Stud attachments are often too adhesive and too susceptible to damage for use in geriatric patients, especially when implants are angulated. This study aimed to evaluate alternative anchoring systems comprising individual and prefabricated polyvinylsiloxane (PVS) attachments. MATERIALS AND METHODS:A total o...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-018-2568-x

    authors： Schweyen R,Arnold C,Setz JM,Hey J

    更新日期：2019-03-01 00:00:00

  • Reconstruction of the alveolar cleft: can growth factor-aided tissue engineering replace autologous bone grafting? A literature review and systematic review of results obtained with bone morphogenetic protein-2.

    abstract：:The alveolar cleft in patients with clefts of lip, alveolus and palate (CLAP) is usually reconstructed with an autologous bone graft. Harvesting of autologous bone grafts is associated with more or less donor site morbidity. Donor site morbidity could be eliminated if bone is fabricated by growth factor-aided tissue e...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,评审

    doi：10.1007/s00784-011-0547-6

    authors： van Hout WM,Mink van der Molen AB,Breugem CC,Koole R,Van Cann EM

    更新日期：2011-06-01 00:00:00

  • Clinical evaluation of the performance and safety of a new dentine substitute, Biodentine, in the restoration of posterior teeth - a prospective study.

    abstract：OBJECTIVES:A multicentric randomized, 3-year prospective study was conducted to determine for how long Biodentine, a new biocompatible dentine substitute, can remain as a posterior restoration. MATERIALS AND METHODS:First, Biodentine was compared to the composite Z100®, to evaluate whether and for how long it could be...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1007/s00784-012-0701-9

    authors： Koubi G,Colon P,Franquin JC,Hartmann A,Richard G,Faure MO,Lambert G

    更新日期：2013-01-01 00:00:00

  • Ten-year survival of ART restorations in permanent posterior teeth.

    abstract：:This study evaluated the 10-year clinical performance of high-viscosity glass-ionomer cement placed in posterior permanent teeth by means of the Atraumatic Restorative Treatment (ART) approach. One operator placed 167 single- and 107 multiple-surface restorations in 43 high-risk caries pregnant women (mean decayed tee...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00784-009-0378-x

    authors： Zanata RL,Fagundes TC,Freitas MC,Lauris JR,Navarro MF

    更新日期：2011-04-01 00:00:00

  • Comparison of indirect pulp treatment and iRoot BP Plus pulpotomy in primary teeth with extremely deep caries: a prospective randomized trial.

    abstract：OBJECTIVES:The purpose of this randomized controlled trial was to compare the 24-month success rates of indirect pulp treatment (IPT) and iRoot BP Plus pulpotomy of primary molars with extremely deep caries. MATERIALS AND METHODS:Generally healthy children aged 3-7 years requiring general anesthesia for treating prima...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-020-03627-4

    authors： Chen X,Zhang H,Zhong J,Yan W,Lin B,Ding M,Xue S,Xia B

    更新日期：2020-10-15 00:00:00

  • Immediate definitive individualized abutments reduce peri-implant bone loss: a randomized controlled split-mouth study on 16 patients.

    abstract：OBJECTIVES:This paper aims to observe peri-implant time-related vertical bone level and soft tissue changes with immediate, non-detached glass-ceramic (lithium-disilicate) individualized abutments and dis-/reconnections. MATERIALS AND METHODS:Sixteen split-mouth patients received provisionalized immediate definitive i...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00784-017-2136-9

    authors： Erhan Çömlekoğlu M,Nizam N,Çömlekoğlu MD

    更新日期：2018-01-01 00:00:00

  • Relationship between dental erosion, soft drink consumption, and gastroesophageal reflux among Icelanders.

    abstract：:The aim of this study was to determine the prevalence of dental erosion in young Icelandic adults (19-22 years old) and patients with gastroesophageal reflux disease (GERD), in relation to their soft drink consumption and gastroesophageal reflux. Eighty subjects (40 males and 40 females), comprising 57 young adults (m...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-003-0252-1

    authors： Jensdottir T,Arnadottir IB,Thorsdottir I,Bardow A,Gudmundsson K,Theodors A,Holbrook WP

    更新日期：2004-06-01 00:00:00

  • Marginal adaptation of ceramic and composite inlays in minimally invasive mod cavities.

    abstract：OBJECTIVES:This study aims to evaluate the effect of a minimally invasive mesial-occlusal-distal (mod) preparation on the marginal adaptation of ceramic and composite inlays with the aim of saving sound dental substance. MATERIALS AND METHODS:Class II mod cavities were prepared in 50 extracted human molars and randoml...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-013-0988-1

    authors： Zaruba M,Kasper R,Kazama R,Wegehaupt FJ,Ender A,Attin T,Mehl A

    更新日期：2014-01-01 00:00:00

  • Push-out bond strength of three calcium silicate cements to root canal dentine after two different irrigation regimes.

    abstract：OBJECTIVES:The aim of this study was to compare the push-out bond strength of three calcium silicate cements to dentine after two different irrigation regimes. MATERIALS AND METHODS:One hundred eighty 2-mm-thick root sections were prepared. The sections were divided into three main groups (n = 60). In group A, the sec...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-013-1082-4

    authors： Çelik D,Er K,Serper A,Taşdemir T,Ceyhanlı KT

    更新日期：2014-05-01 00:00:00

  • A novel three-dimensional analysis of standardized bone defects by means of confocal scanner and micro-computed tomography.

    abstract：OBJECTIVES:The aim of the study was to introduce a novel three-dimensional (3D) method to quantify the relative amount of different tissue components in bone substitute-treated defects by means of integration of confocal laser imaging into micro-computed tomography (μCT) analysis. MATERIALS AND METHODS:One standardize...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-013-1081-5

    authors： Clozza E,Obrecht M,Dard M,Coelho PG,Dahlin C,Engebretson SP

    更新日期：2014-05-01 00:00:00

  • Effect of non-surgical periodontal treatment by full-mouth disinfection or scaling and root planing per quadrant in halitosis-a randomized controlled clinical trial.

    abstract：OBJECTIVE:The objective of this study was to compare the effect of one-stage full-mouth disinfection (FMD) and conventional quadrant scaling in four weekly sessions (QS) on periodontal clinical parameters and halitosis among individuals with advanced chronic periodontitis. MATERIALS AND METHODS:In this randomized cont...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00784-016-1959-0

    authors： Silveira JO,Costa FO,Oliveira PAD,Dutra BC,Cortelli SC,Cortelli JR,Cota LOM,Oliveira AMSD

    更新日期：2017-06-01 00:00:00

  • Effectiveness of metronidazole gel on cyclosporine-induced gingival overgrowth in heart transplant patients.

    abstract：:To evaluate the efficacy of metronidazole on cyclosporine-induced gingival overgrowth (GO), a prospective intra-subject double-blind longitudinal study was performed on six heart transplant patients with GO. All patients underwent scaling and root planing before any treatment. Metronidazole gel (Elyzol, Cabon) was the...

    journal_title：Clinical oral investigations

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1007/s00784-001-0142-3

    authors： Montebugnoli L,Servidio D,Prati C

    更新日期：2002-03-01 00:00:00

  • Oral verrucous carcinoma: a retrospective study in São Paulo Region, Brazil.

    abstract：:Oral verrucous carcinoma (OVC) is a rare variant of squamous cell carcinoma with a characteristic morphology and specific behavior. To date, few studies are available focusing the prevalence and clinicopathologic features of the oral verrucous carcinoma in Brazilian population. A total of 3,500 primary oral well-diffe...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-006-0050-7

    authors： Oliveira DT,de Moraes RV,Fiamengui Filho JF,Fanton Neto J,Landman G,Kowalski LP

    更新日期：2006-09-01 00:00:00

  • Polymerization pattern characterization within a resin-based composite cured using different curing units at two distances.

    abstract：OBJECTIVES:To investigate the relationship of the irradiance-beam-profile areas from six different light-curing units (LCUs) with the degree of conversion (DC), microhardness (KH), and cross-link density (CLD) throughout a resin-based composite (RBC) cured at two clinically relevant distances, and to explore the correl...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-019-02831-1

    authors： Al-Zain AO,Eckert GJ,Lukic H,Megremis S,Platt JA

    更新日期：2019-11-01 00:00:00

  • Squamous cell carcinoma of the oral cavity: a case series analysis of clinical presentation and histological grading of 1,425 cases from Iraq.

    abstract：:Peoples in Iraq face a mixture of health hazards associated with poverty. Oral cancer is a major public health issue worldwide; it remains a highly lethal and disfiguring disease. It is primarily a disease of epithelial origin. A total of 1,425 cases of histologically diagnosed squamous cell carcinoma collected from t...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-007-0141-0

    authors： Al-Rawi NH,Talabani NG

    更新日期：2008-03-01 00:00:00

  • Assessment of postoperative pain after reciprocating or rotary NiTi instrumentation of root canals: a randomized, controlled clinical trial.

    abstract：OBJECTIVES:The aim of this study was to assess postoperative pain in a prospective randomized clinical trial comparing two groups, using the Reciproc® system in one group and the ProTaper® rotary system in the other. MATERIAL AND METHODS:The study included 78 male patients, aged 18-64 years (mean age of 26 years), wit...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00784-015-1692-0

    authors： Relvas JB,Bastos MM,Marques AA,Garrido AD,Sponchiado EC Jr

    更新日期：2016-11-01 00:00:00

  • Screening and diagnosis of oral cancer: a critical quality appraisal of clinical guidelines.

    abstract：OBJECTIVES:To assess the quality of clinical practice guidelines (CPGs) on screening and diagnosis of oral cancer and to describe the characteristics of their recommendations. MATERIALS AND METHODS:We systematically searched EMBASE, MEDLINE, CPG' websites, and dentistry and oncology scientific societies to identify CP...

    journal_title：Clinical oral investigations

    pub_type： 杂志文章

    doi：10.1007/s00784-018-2668-7

    authors： Madera M,Franco J,Solà I,Bonfill X,Alonso-Coello P

    更新日期：2019-05-01 00:00:00