Abstract:
OBJECTIVES:The aim of the present study was to investigate different fluorescence-based, two-color viability assays for visualization and quantification of initial bacterial adherence and to establish reliable alternatives to the ethidium bromide staining procedure. MATERIALS AND METHODS:Bacterial colonization was attained in situ on bovine enamel slabs (n = 6 subjects). Five different live/dead assays were investigated (fluorescein diacetate (FDA)/propidium iodide (PI), Syto 9/PI (BacLight®), FDA/Sytox red, Calcein acetoxymethyl (AM)/Sytox red, and carboxyfluorescein diacetate (CFDA)/Sytox red). After 120 min of oral exposure, analysis was performed with an epifluorescence microscope. Validation was carried out, using the colony-forming units for quantification and the transmission electron microscopy for visualization after staining. RESULTS:The average number of bacteria amounted to 2.9 ± 0.8 × 10(4) cm(-2). Quantification with Syto 9/PI and Calcein AM/Sytox red yielded an almost equal distribution of cells (Syto 9/PI 45% viable, 55% avital; Calcein AM/Sytox red 52% viable, 48% avital). The live/dead ratio of CFDA/Sytox red and FDA/Sytox red was 3:2. An aberrant dispersal was recorded with FDA/PI (viable 34%, avital 66%). The TEM analysis indicated that all staining procedures affect the structural integrity of the bacterial cells considerably. CONCLUSION:The following live/dead assays are reliable techniques for differentiation of viable and avital adherent bacteria: BacLight, FDA/Sytox red, Calcein AM/Sytox red, and CFDA/Sytox red. These fluorescence-based techniques are applicable alternatives to toxic and instable conventional assays, such as the staining procedure based on ethidium bromide. CLINICAL RELEVANCE:Differentiation of viable and avital adherent bacteria offers the possibility for reliable evaluation of different mouth rinses, oral medication, and disinfections.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Tawakoli PN,Al-Ahmad A,Hoth-Hannig W,Hannig M,Hannig Cdoi
10.1007/s00784-012-0792-3subject
Has Abstractpub_date
2013-04-01 00:00:00pages
841-50issue
3eissn
1432-6981issn
1436-3771journal_volume
17pub_type
杂志文章abstract:OBJECTIVE:This study aimed to prospectively observe gustatory and neurosensory alterations following surgical removal of mandibular third molars. MATERIAL AND METHODS:A prospective clinical study was conducted with patients who required mandibular third molar extraction, recruited from the Division of Oral and Maxillo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-02798-5
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:The caries preventive effect of long-term use (1 year) of low-dosage (2.5 g/die) of xylitol chewing gum in a high-caries-risk adult population was evaluated. MATERIALS AND METHODS:In this randomized clinical trial, 179 high-caries-risk adults were assigned to two experimental groups, xylitol and polyols. Ca...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
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更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:Intrapapillary injection (IPI) has been suggested to improve pulpal anesthesia of mandibular teeth and to avoid complications from inferior alveolar nerve block (IANB). This study aimed to determine and compare clinical efficacies and prostaglandin E2 (PGE2) levels between IPI and IANB. MATERIALS AND METHODS...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
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更新日期:2020-02-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0939-x
更新日期:2014-01-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
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更新日期:2008-06-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03514-y
更新日期:2020-08-22 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03416-z
更新日期:2021-02-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2594-8
更新日期:2019-04-01 00:00:00
abstract:: ...
journal_title:Clinical oral investigations
pub_type: 评论,信件
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更新日期:2019-01-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2228-6
更新日期:2018-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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更新日期:2019-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
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更新日期:2020-08-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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更新日期:2016-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-007-0124-1
更新日期:2007-12-01 00:00:00
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doi:10.1007/s00784-015-1497-1
更新日期:2016-01-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-021-03788-w
更新日期:2021-01-18 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0347-4
更新日期:2010-12-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1105-1
更新日期:2014-05-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1167-0
更新日期:2014-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00784-018-2512-0
更新日期:2018-09-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
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更新日期:2016-03-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1798-z
更新日期:2017-01-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03267-8
更新日期:2020-05-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2308-7
更新日期:2018-07-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1465-9
更新日期:2016-01-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-011-0642-8
更新日期:2012-10-01 00:00:00
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journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-019-02910-3
更新日期:2020-01-01 00:00:00