当前位置： SCI文献检索 > Clinical Oral Investigations期刊下所有文献 > Gene expression profile of compressed primary human cementoblasts before and after IL-1β stimulation.

Gene expression profile of compressed primary human cementoblasts before and after IL-1β stimulation.

Abstract：

OBJECTIVES:Root resorptions due to a reduced repair function of cementoblasts are common side effects during orthodontic tooth movement (OTM). The mechanisms, which lead to an impaired cementoblast function, are not fully understood. Therefore, we aimed to investigate changes in the gene expression of cementoblasts during mechanical stimulus under inflammatory conditions. MATERIALS AND METHODS:Human primary cementoblasts (HPCB) were exposed to compression for 6 h or stimulation with IL-1β for 96 h and subsequent 6 h compression. Genome-wide expression analysis was performed using microarray analysis. Prominent gene expression alterations (COX2, AXUD1, FOSB, CCL2, IFI6, and PTGES) were verified by quantitative RT-PCR (qRT-PCR) in two HPCB populations. A caspase 3/7 activity assay was used to determine caspase-3 and caspase-7 activity in stressed cells. RESULTS:Gene expression cluster analysis revealed apoptosis as an important process induced under both conditions. Apoptosis (pro- and anti-apoptotic) related gene expression was most relevant after pro-inflammatory stimulation and compression. qRT-PCR analysis confirmed significant up-regulation of COX2, AXUD1, and FOSB in both HPCB populations after compression, while selected genes significantly increased after pro-inflammatory stimulation and compression. Compression of cementoblasts increased caspase. The combination of pro-inflammatory stimulation and compression led to a slightly smaller increase of caspase activity. CONCLUSIONS:Gene ontology analysis showed that compressed HPCB up-regulate genes that are associated with apoptosis. Combining compression with a pro-inflammatory stimulus (IL-1β) augmented the positive regulation of apoptosis-related pathways. The induction of apoptosis related gene expression (pro- and anti-apoptotic genes) in cementoblasts suggests an involvement of apoptosis in cementoblast regulation during OTM. CLINICAL RELEVANCE:As apoptosis is induced in HPCB after compression and inflammation, it is conceivable that HPCB cell death might contribute to root resorptions due to a loss of repair activity of cementoblasts. Further studies should be conducted to clarify the implication of the identified genes on root resorptions in order to develop therapeutic strategies to prevent a shortening of roots.

journal_name

Clin Oral Investig

journal_title

Clinical oral investigations

authors

Diercke K,Zingler S,Kohl A,Lux CJ,Erber R

doi

10.1007/s00784-013-1167-0

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

1925-39

issue

eissn

1432-6981

issn

1436-3771

journal_volume

pub_type

杂志文章

在线工具

Gene expression profile of compressed primary human cementoblasts before and after IL-1β stimulation.