Abstract:
OBJECTIVES:Intermittent parathyroid hormone (PTH) exerts anabolic effects on bone and has been approved for osteoporosis therapy. The dual actions of PTH are mediated primarily through the parathyroid hormone 1 receptor (PTH1R). Upon ligand binding, PTH1R activates diverse signaling pathways, including cAMP/protein kinase A (PKA)- and phospholipase C/protein kinase C (PLC/PKC)-dependent pathways. PTH1R has been abundantly studied in bone cells. Knowledge on PTH1R characteristics and physiology in periodontal ligament (PDL) cells is still in its infancy. MATERIALS AND METHODS:We characterized PTH1R in PDL cells in terms of its cellular localization, binding affinity, and signal transduction and compared these characteristics to those of MG63 osteoblast-like cells. RESULTS:PTH1R mRNA/protein was identified in PDL and MG63 cells. PTH1R was mainly localized on the plasma membrane, in vesicular structures inside the cell, and, to some extent, in the nucleus of both cell types. Binding characteristics of PTH1R were cell type specific, with PDL cells demonstrating a lower binding affinity. The response of cAMP and active PKC production in MG63 cells was dose dependent with increasing PTH(1-34) concentration, whereas in PDL cells, it was regulated biphasically. However, we observed a cross talk between the cAMP/PKA and PLC/PKC signaling pathways, which were regulated diametrically opposed at a given concentration of PTH(1-34). CONCLUSION:These data indicate that, albeit the similarity in its subcellular distribution, PTH1R in PDL cells exhibits characteristics different from those in MG63 cells, pointing to the cell type specificity of this receptor. CLINICAL RELEVANCE:The findings further elucidate the characteristics of PTH action in dental tissues and widen the theoretical basis for the development of anabolic treatment strategies.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Abuduwali N,Lossdörfer S,Winter J,Kraus D,Guhlke S,Wolf M,Jäger Adoi
10.1007/s00784-013-0985-4subject
Has Abstractpub_date
2014-01-01 00:00:00pages
461-70issue
2eissn
1432-6981issn
1436-3771journal_volume
18pub_type
杂志文章abstract:OBJECTIVES:The aim of this study was to examine reports of perceptual distortion evoked by transient deafferentation and burning pain as models of aspects of burning mouth syndrome (BMS). MATERIALS AND METHODS:Sixteen healthy women took part in three experimental sessions that included exposure to lingual nerve block,...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1994-x
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the contamination level of toothbrushes by mutans streptococci (MS) and the efficacy of antimicrobial solutions: cetylpyridinium chloride 0.05 % (CPC; Cepacol™) and chlorhexidine 0.12 % (CHX; Periogard™), to disinfect toothbrushes of preschool-aged children in day-care centers. MATERIAL AND METHO...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-013-1169-y
更新日期:2014-11-01 00:00:00
abstract:OBJECTIVES:To explore whether a computed tomography (CT) examination of the head and neck region induces biological damage and whether the damage was correlated with the radiation dose. MATERIALS AND METHODS:Peripheral blood was taken from 33 individuals who received head and neck CT examinations. Blood samples were d...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03331-3
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Bisphosphonates and denosumab are antiresorptive drugs used for the treatment of osteoporosis and oncological tumors. A severe side effect is osteonecrosis of the jaw. Monocyte/macrophage dysfunction is considered to play a distinct role in osteonecrosis. THP-1 monocytic cells were used in this study to eluc...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1358-3
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVES:Previous research revealed that autogenous tooth roots may be biologically equivalent to conventional bone grafts for lateral ridge augmentation. However, these analyses were limited to two dimensions, whereas healing is a volumetric process. The present study aimed at volumetrically assessing the microstruc...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2723-4
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVE:Our aim was to assess the anti-biofilm ability of previously unverified individual D-amino acids (DAAs), to produce plasma polymer encapsulated DAAs (PPEDAAs), to measure the shell thickness and subsequent release of DAAs, and to assess the effects of PPEDAAs on Enterococcus faecalis biofilms. MATERIALS AND ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03663-0
更新日期:2020-11-02 00:00:00
abstract:OBJECTIVES:To investigate the relationship of the irradiance-beam-profile areas from six different light-curing units (LCUs) with the degree of conversion (DC), microhardness (KH), and cross-link density (CLD) throughout a resin-based composite (RBC) cured at two clinically relevant distances, and to explore the correl...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02831-1
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:Clinicians agree that children with isolated cleft lip have fewer cleft-associated problems than children with cleft lip and palate. Unfortunately, for isolated cleft lip children, the risk of cleft-associated problems is unknown and maybe underestimated. Often, these children do not get the required follow-...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03367-5
更新日期:2020-06-04 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. MATERIALS AND METHODS:The following strains were tested: Bifidobacterium dentium DSM20436, Parascardovia denticolens DSM10105, and Scardovia inopinata DSM10107. Streptococcus mutans UA...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1958-1
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:The present study evaluated the cellular tissue reaction of two equine-derived collagen hemostatic sponges (E-CHS), which differed in thickness after pressing, over 30 days in vivo. The inflammatory response during physiological wound healing in sham-operated animals was used as control group. MATERIAL AND ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03219-2
更新日期:2020-10-01 00:00:00
abstract::From a preventive point of view collection of data concerning carious and non-carious cervical tooth defects is definitely important. Consequently, the prevalence and distribution of different root lesions were studied and correlated with behavioral and biological factors in 50- to 60-year-old German individuals (n = ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050097
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVE:Azithromycin is a macrolide antibiotic that appears to have both antibacterial and anti-inflammatory properties. This study aimed to investigate the effect of azithromycin on the production of pro-inflammatory cytokines and chemokines by human gingival fibroblasts (HGF) in vitro. MATERIALS AND METHODS:The ef...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1249-7
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:Investigate the biochemistry of in vivo healthy oral tissues through Raman spectroscopy. We aimed to characterize the biochemical features of healthy condition in oral subsites (buccal mucosa, lip, tongue, and gingiva) of healthy subjects. More specifically, we investigated Raman spectral characteristics and...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2714-5
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:To evaluate and compare the obturation quality of mineral trioxide aggregate (MTA) and Biodentine placed with hand condensation or indirect ultrasonic activation technique in teeth models simulating perforating internal root resorption (IRR) using micro-computed tomographic (micro-CT) imaging. MATERIALS AND...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2502-2
更新日期:2019-02-01 00:00:00
abstract::The aim of the study was to investigate the temperature rise of a nanocomposite and a conventional hybrid dental composite during photopolymerization when cured with halogen curing lamp (QHT) and light-emitting diode (LED). Temperature rise during photopolymerization of two commercially available composites (Filtek Su...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-008-0236-2
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVES:This study aims to evaluate the effect of a minimally invasive mesial-occlusal-distal (mod) preparation on the marginal adaptation of ceramic and composite inlays with the aim of saving sound dental substance. MATERIALS AND METHODS:Class II mod cavities were prepared in 50 extracted human molars and randoml...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0988-1
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:Resin polymerisation shrinkage reduces the congruence of the denture base with denture-bearing tissues and thereby decreases the retention of conventionally fabricated dentures. CAD/CAM denture manufacturing is a subtractive process, and polymerisation shrinkage is not an issue anymore. Therefore, CAD/CAM de...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2369-2
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:This study aimed to estimate the dental age (DA) using the London Atlas, Willems, and Nolla methods and to compare their accuracies. METHODS:The DA was estimated using the aforementioned methods on panoramic radiograms of a total of 919 Eastern Turkish patients aged 6-14 years, of whom 459 were female and 4...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-021-03788-w
更新日期:2021-01-18 00:00:00
abstract:OBJECTIVES:To investigate the relationship between sleep disorders, morning hyposalivation, and subjective feeling of dry mouth. MATERIALS AND METHODS:A cross-sectional, observational, clinical study was carried out in a homogenous population sample which consists of Greek male soldiers without any medical history. Af...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03029-1
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation. MATERIALS AND METHODS:Thirty-six rats' right incisors were used. Teeth were extracted and divided into: del...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03430-1
更新日期:2020-07-29 00:00:00
abstract:OBJECTIVE:The aim of this article is to compare the influence of surface treatment on the integration (at 2, 4 and 8 weeks) of 120 dental implants inserted in 60 tibiae of rabbits. MATERIALS AND METHODS:Four different surfaces were double-blind tested: blasted, acid-etched and discrete crystal deposition (DCD) (group ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1241-2
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:An optimized oral health-related section and a video training were developed and validated for the interRAI suite of instruments. The latter is completed by professional non-dental caregivers and used in more than 40 countries to assess care needs of older adults. METHODS:The optimized oral health-related s...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03669-8
更新日期:2020-11-16 00:00:00
abstract::The aim of this study was to evaluate the influence of caries-detecting dyes on the microleakage of adhesive materials. Sixty cubic class V cavities were prepared on buccal and lingual surfaces of 30 human third molars. Coronal margins were located in enamel and gingival margins in cementum. The teeth were randomly di...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-002-0182-3
更新日期:2002-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to compare wound healing and patient pain perception of single-incision (single-incision, modified single-incision) and trap-door surgical techniques to harvest subepithelial connective tissue grafts from the palate. MATERIAL AND METHODS:Thirty-six patients were selected for root co...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1204-7
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVES:The purpose of the present study was to evaluate the frequency, locations, and dimensions of mucous retention cysts of the maxillary sinus and analyze potential associated dental pathology. MATERIALS AND METHODS:A total of 156 cone beam computed tomography (CBCT) scans were included in the analysis, resulti...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2206-z
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:This study aims to investigate the additional influence of multiple applications of antimicrobial photodynamic therapy (aPDT) in smokers with chronic periodontitis. MATERIALS AND METHODS:Twenty smokers with chronic periodontitis were treated in a split-mouth design study with aPDT adjunct to Scaling and Roo...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2740-3
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVES:Advanced oral cancer is a major public health concern because of a lack of effective prevention and treatment. Triptolide (TPL), a diterpenoid triepoxide derived from the Chinese herb Tripterygium wilfordii, has been demonstrated to possess strong anticancer properties. In this study, we investigated whether...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1808-1
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVES:The present dental bleaching case report describes a new method that precisely quantifies the daily wearing-times of the bleaching product by inserting a microsensor in the acetate custom tray. The bleaching efficacy was also discussed since the patient was previously submitted to enamel microabrasion. METH...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2439-5
更新日期:2019-01-01 00:00:00
abstract::The objective of this study is to determine if there is an association between HIV infection in children and alterations in the chronological development of permanent dentition detectable by radiographic examination. The sample comprised two groups of children: (1) 50 children infected with HIV by vertical transmissio...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-007-0124-1
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the association between salivary and plaque arginine levels/ADS activities with dental caries. MATERIALS AND METHODS:A systematic search was performed as per PRISMA statement using PubMed, Scopus, Cochrane Library, and Web of Science. Published studies that invest...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00784-017-2322-9
更新日期:2018-03-01 00:00:00