Abstract:
OBJECTIVES:Bisphosphonates and denosumab are antiresorptive drugs used for the treatment of osteoporosis and oncological tumors. A severe side effect is osteonecrosis of the jaw. Monocyte/macrophage dysfunction is considered to play a distinct role in osteonecrosis. THP-1 monocytic cells were used in this study to elucidate the influence of zoledronate and denosumab on phorbol-12-myrisate-13-acetate (PMA)-induced macrophage differentiation and function in real-time. MATERIALS AND METHODS:Macrophagic differentiation of the THP-1 suspension cells was measured by cell adherence in the presence or absence of different concentrations of zoledronate (0.5, 5, 50 μM) and denosumab (1, 10, 20, 40 μg/mL) using the real-time xCELLigence system. Additionally, a live/dead staining was performed by fluorescence microscopy. RESULTS:THP-1 cells demonstrated a regular initial PMA-induced differentiation to macrophages by live measurements of cell adherence and by an increase in CD68 surface expression as detected by flow cytometry. The addition of zoledronate led to cell detachment of the THP-1-derived macrophages in a dose-dependent manner in contrast to denosumab. Cell detachment was based on cell death as confirmed by live/dead staining, revealing elevated numbers of dead cells following addition of high zoledronate concentrations. However, denosumab did not deteriorate THP-1 cell viability. CONCLUSION:Our results demonstrate that zoledronate but not denosumab suppresses monocytic THP-1 cell viability after macrophagic differentiation dose-dependently. CLINICAL RELEVANCE:This is the first real-time study providing evidence for a dose-dependent immunosuppressive effect of zoledronate in contrast to denosumab on local macrophages.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Hoefert S,Hoefert CS,Albert M,Munz A,Grimm M,Northoff H,Reinert S,Alexander Ddoi
10.1007/s00784-014-1358-3subject
Has Abstractpub_date
2015-07-01 00:00:00pages
1307-18issue
6eissn
1432-6981issn
1436-3771journal_volume
19pub_type
杂志文章abstract:OBJECTIVES:The purpose of the present study was to evaluate the frequency, locations, and dimensions of mucous retention cysts of the maxillary sinus and analyze potential associated dental pathology. MATERIALS AND METHODS:A total of 156 cone beam computed tomography (CBCT) scans were included in the analysis, resulti...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2206-z
更新日期:2018-04-01 00:00:00
abstract::This study evaluated the colour parameters of composite and ceramic shade guides determined using a colorimeter and digital imaging method with illuminants at different colour temperatures. Two different resin composite shade guides, namely Charisma (Heraeus Kulzer) and Premise (Kerr Corporation), and two different ce...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-009-0329-6
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP). MATERIALS AND METHODS:We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxill...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1166-1
更新日期:2014-11-01 00:00:00
abstract:OBJECTIVES:To evaluate the diagnostic efficacy of CBCT-MRI fused images for articular disc calcification of temporomandibular joint (TMJ). MATERIALS AND METHODS:Twenty patients (24 TMJs) whose image examinations showed dense bodies in the TMJ space were included in the study. The locations of dense bodies evaluated by...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03497-w
更新日期:2020-08-12 00:00:00
abstract:OBJECTIVES:Determine the association between sialadenitis and osteonecrosis of the jaw (ONJ) in head and neck cancer (HNC) patients with varying severity of sialadenitis, treatment modalities, and cancer locations. MATERIALS AND METHODS:A total of 40,168 HNC patients, including 1907 ONJ subjects and 7559 matched compa...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2465-3
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:Dental materials and oral hygiene products may be responsible for oral contact allergic reactions. We aimed to determine the occurrence of allergies in patients with symptomatic oral lichen planus (OLP), oral lichenoid lesions (OLLs) and stomatitis and investigate if patch testing could identify contact aller...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2053-y
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to compare temperature variation during osteotomies with trephine drills under different irrigation conditions: without irrigation, external irrigation, and double irrigation. MATERIALS AND METHODS:Synthetic blocks of bone (type I density) were used for drilling procedures with ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1163-4
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVES:Cell-free DNA (cfDNA) harboring mutations has been found in patients with diseases. Experimental studies have shown that cfDNA can be transmitted, leading to transformations in the host. In the present study, we evaluated whether bone allograft material contains cfDNA and whether this foreign cfDNA can be re...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02867-3
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:To compare selective COX-2 inhibitors with ibuprofen in terms of analgesia, rescue medication consumption, and adverse effects after impacted third molar removal. MATERIALS AND METHODS:Electronic databases were searched. Single dose, double-blind, randomized, and controlled clinical trials comparing the ana...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-019-02910-3
更新日期:2020-01-01 00:00:00
abstract:: ...
journal_title:Clinical oral investigations
pub_type: 评论,信件
doi:10.1007/s00784-018-2636-2
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to measure the percentage of volume of voids and gaps in the apical third of root canals obturated with two techniques using micro-computed tomography. MATERIALS AND METHODS:Fifty-four single-rooted teeth were collected and root canal-prepared. The roots were randomly allocated into...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0875-1
更新日期:2013-11-01 00:00:00
abstract::This study evaluated the 10-year clinical performance of high-viscosity glass-ionomer cement placed in posterior permanent teeth by means of the Atraumatic Restorative Treatment (ART) approach. One operator placed 167 single- and 107 multiple-surface restorations in 43 high-risk caries pregnant women (mean decayed tee...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0378-x
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:Different studies suggest that inflammation as well as hypoxia leads to an increase of p53 protein levels. However, the implication of p53 during oral inflammatory processes is still unknown. The aim of this study was therefore to investigate the effect of hypoxia and inflammation on p53 regulation in human ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1679-x
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate factors associated with the incidence of erosive tooth wear (ETW) among adults at different ages in Tokyo using multifactorial logistic regression analysis. MATERIALS AND METHODS:The study sample consisted of a total of 1108 subjects aged 15 to 89 years in Tokyo, Japan....
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-017-2065-7
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVES:To investigate the relationship of the irradiance-beam-profile areas from six different light-curing units (LCUs) with the degree of conversion (DC), microhardness (KH), and cross-link density (CLD) throughout a resin-based composite (RBC) cured at two clinically relevant distances, and to explore the correl...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02831-1
更新日期:2019-11-01 00:00:00
abstract::The present study focuses on the correlation between the expression pattern of β-catenin (component of Wnt signaling), ΔNp63 (proliferation marker), and Notch 1 (transmembrane receptor) in oral squamous cell carcinoma. The study also aims to investigate the interaction between β-catenin and ΔNp63 in oral cancer. Furth...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-011-0605-0
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the push-out bond strength of three calcium silicate cements to dentine after two different irrigation regimes. MATERIALS AND METHODS:One hundred eighty 2-mm-thick root sections were prepared. The sections were divided into three main groups (n = 60). In group A, the sec...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1082-4
更新日期:2014-05-01 00:00:00
abstract::Regular screening through white light inspection of the entire oral mucosa is the most important examination method to identify precancerous lesions and early oral carcinoma. Additionally, the physiologic autofluorescence of the oral mucosa has been described as a novel screening method for the detection of mucosal le...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章
doi:10.1007/s00784-010-0455-1
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVES:Pain and trismus are the most common postoperative complications following a surgical removal of the lower third molar. This randomized, double-blind, placebo-controlled clinical trial evaluated the effect of orally administrated magnesium on postoperative pain and trismus after surgical removal of the lower...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-020-03335-z
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:Although dental trauma and its unfavorable sequelae are considered major public health problems worldwide, the published data on the prevalence of traumatic crown injuries (TCIs) in Germany are lacking. Therefore, the present study assessed the prevalence of TCIs among adolescents in Bavaria, Germany. MATER...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02974-1
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:This study compared the effects of mold material and diameter on the thickness of cured composite remnants and depth of cure (DOC) of resin-based composites (RBC). MATERIAL AND METHODS:One Polywave® curing light was used to photo-cure two shades of the same "bulk-fill" RBC in 4, 6, or 10-mm internal diameter...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1672-4
更新日期:2016-09-01 00:00:00
abstract::The aims of this paper were to critically review the role of radiographic imaging before lower third molar removal and to suggest a strategy for preoperative imaging based on available scientific evidence and clinical experience. Original articles and reviews including the MESH terms "third molar" and "radiography" we...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-008-0200-1
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVES:To assess the perception of oral health and oral care needs, barriers and current practices as perceived by managers and caregivers in organizations for people with disabilities in Flanders. MATERIALS AND METHODS:Two questionnaires were developed, one for managers and one for caregivers. The questionnaires ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03071-z
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:An optimized oral health-related section and a video training were developed and validated for the interRAI suite of instruments. The latter is completed by professional non-dental caregivers and used in more than 40 countries to assess care needs of older adults. METHODS:The optimized oral health-related s...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03669-8
更新日期:2020-11-16 00:00:00
abstract:OBJECTIVES:Previous research revealed that autogenous tooth roots may be biologically equivalent to conventional bone grafts for lateral ridge augmentation. However, these analyses were limited to two dimensions, whereas healing is a volumetric process. The present study aimed at volumetrically assessing the microstruc...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2723-4
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:This study aimed to investigate the effect of fracture orientation on the detection accuracy of vertical root fractures (VRFs) in non-endodontically treated teeth using four different cone beam computed tomography (CBCT) units. MATERIALS AND METHODS:Thirty eight out of 148 extracted human permanent teeth we...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02905-0
更新日期:2019-12-01 00:00:00
abstract::Dear Professor Dr. Matthias Hannig. Editor-in-Chief Clinical Oral Investigations. Following the publication of our paper by Calvo Guirado et al, 2015 [1] in Clinical Oral Investigations, it came to light that a certain part of the text at the materials and methods and results sections were similar. ...
journal_title:Clinical oral investigations
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00784-018-2763-9
更新日期:2019-01-01 00:00:00
abstract::The purpose of the present study was to decide whether composite resin or conventional glass ionomer cement should be preferred as a base material in endodontically treated premolars. Twelve extracted human maxillary premolars were mounted in a universal testing machine at a 35 degrees angle. Cuspal stiffness was dete...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s007840050049
更新日期:1998-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the frequency and quality of root canal fillings (RCF) and the prevalence of apical radiolucency (AR) in a German population. MATERIAL AND METHODS:Five hundred randomly selected full-size CBCT images (Planmeca ProMax 3D, FOV = 8 × 8 cm, voxel size 160 μm or 200 μm) wer...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02985-y
更新日期:2020-03-01 00:00:00
abstract::The aims of the present study were: a) to assess the impact of the intraoral location on the rate of biofilm growth, and b) to establish an in vivo biofilm model to examine intraoral biofilm growth. Eight healthy volunteers wore acrylic splints with 15 glass slabs each in the upper and lower jaws to build up plaque. A...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-004-0255-6
更新日期:2004-06-01 00:00:00