Abstract:
:Background: Castration-resistant prostate cancer (CRPC) is an incurable malignancy. Long noncoding RNAs (lncRNAs) play key roles in drug resistance. Materials & methods: LncRNA HORAS5 role in cabazitaxel resistance (i.e., cell-count, IC50 and caspase activity) was studied via lentiviral-mediated overexpression and siRNA-based knockdown. Genes expression was analyzed with RNA-sequencing, reverse transcription quantitative PCR (RT-qPCR) and western blot. HORAS5 expression was queried in clinical database. Results: Cabazitaxel increased HORAS5 expression that upregulated BCL2A1, thereby protecting CRPC cells from cabazitaxel-induced apoptosis. BCL2A1 knockdown decreased cell-count and increased apoptosis in CRPC cells. HORAS5-targeting antisense oligonucleotide decreased cabazitaxel IC50. In CRPC clinical samples, HORAS5 expression increased upon taxane treatment. Conclusion:HORAS5 stimulates the expression of BCL2A1 thereby decreasing apoptosis and enhancing cabazitaxel resistance in CRPC cells.
journal_name
Epigenomicsjournal_title
Epigenomicsauthors
Pucci P,Venalainen E,Alborelli I,Quagliata L,Hawkes C,Mather R,Romero I,Rigas SH,Wang Y,Crea Fdoi
10.2217/epi-2019-0316subject
Has Abstractpub_date
2020-07-01 00:00:00pages
1123-1138issue
13eissn
1750-1911issn
1750-192Xjournal_volume
12pub_type
杂志文章相关文献
Epigenomics文献大全abstract::Epigenetic modifications established during gametogenesis regulate transcription and other nuclear processes in gametes, but also have influences in the zygote, embryo and postnatal life. This is best understood for DNA methylation which, established at discrete regions of the oocyte and sperm genomes, governs genomic...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi-2016-0056
更新日期:2016-10-01 00:00:00
abstract::Aim: To develop a method for estimating cell-specific effects in epigenomic association studies in the presence of cell type heterogeneity. Materials & methods: We utilized Monte Carlo Expectation-Maximization (MCEM) algorithm with Metropolis-Hastings sampler to reconstruct the 'missing' cell-specific methylations and...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0147
更新日期:2020-12-22 00:00:00
abstract:AIM:In order to identify regulators of foam cells, we studied the H3K27 demethylase Kdm6b (also known as Jmjd3), a known regulator of macrophages, in controlling the transcriptional profile of foam cells. MATERIALS & METHODS:Foam cells from Kdm6b-deleted or Kdm6b wild-type mice were isolated and used for RNA-sequencin...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0152
更新日期:2017-04-01 00:00:00
abstract::Aim: Epilepsy is a common neurological disorder characterized by recurrent seizures. We performed epigenetic analyses between and within 15 monozygotic (MZ) twin pairs discordant for focal or generalized epilepsy. Methods: DNA methylation analysis was performed using Illumina Infinium MethylationEPIC arrays, in blood ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2018-0136
更新日期:2019-06-01 00:00:00
abstract::This article focuses on the epigenetic alterations of aberrant promoter hypermethylation of genes, and histone modifications or RNA interference in cancer cells. Current knowledge of the hypermethylation of allele(s) in classical tumor suppressor genes in inherited and sporadic cancer, candidate tumor suppressor and o...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.10.64
更新日期:2011-02-01 00:00:00
abstract:AIM:We examined maternal prepregnancy anthropometry and cord blood DNA methylation. METHODS:Associations between maternal measures (i.e., weight, height, waist circumference, hip circumference, skinfolds, leptin) and methylation β-values at each CpG (measured by the Infinium MethylationEPIC BeadChip) were estimated am...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2018-0099
更新日期:2019-02-01 00:00:00
abstract::Aim: To examine the impact of 4-nonylphenol (4-NP), on the expression of polycomb repressive complexes and cellular proliferation. Materials & methods: Cell proliferation assays, quantitative PCR, Western blotting, luciferase reporter assay, chromatin immunoprecipitation-quantitative PCR were used for the study. Resul...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2018-0175
更新日期:2019-06-01 00:00:00
abstract:AIM:To identify regions of aberrant DNA methylation in acute lymphoblastic leukemia (ALL) cells of different subtypes on a genome-wide scale. MATERIALS & METHODS:Whole-genome bisulfite sequencing (WGBS) was used to determine the DNA methylation levels in cells from four pediatric ALL patients of different subtypes. Th...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0052
更新日期:2016-10-01 00:00:00
abstract::Aim: To determine changes in global DNA methylation in monocytes from neonates of women with obesity, as markers of an immune programming resulting from maternal obesity. Materials & methods: Cord blood monocytes were obtained from neonates born to women with obesity and normal weight, genome-wide differentially methy...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0098
更新日期:2020-11-01 00:00:00
abstract::Aim: To identify pregnancy-associated changes in cervical noncoding RNA (ncRNA), including miRNA and long noncoding RNA (lncRNA), and their potential effects on biologic processes. Materials & methods: We enrolled 21 pregnant women with term deliveries (≥37 weeks' gestation) in a prospective cohort and collected cervi...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2019-0231
更新日期:2020-06-01 00:00:00
abstract::Protein methyltransferases (PMT)s play essential roles in many biological processes through methylation of histones and diverse nonhistone substrates. Dysregulation of these enzymes has been implicated in many diseases including cancers. While PMT-associated biology can be probed via genetic perturbation, this approac...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.15.87
更新日期:2015-01-01 00:00:00
abstract::Prader-Willi syndrome (PWS) is a relatively rare disorder that originates from paternally inherited deletions and maternal disomy (mUPD) within the 15q11-q13 region or alterations in the PWS imprinting center. Evidence is accumulating that mUPD underlies high prevalence of psychosis among PWS patients. Several genes i...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.14.52
更新日期:2014-01-01 00:00:00
abstract::Alterations in DNA methylation have been associated with cognitive decline and Alzheimer's disease. A recent study of mild cognitive impairment (MCI) reported a significant association between annual decline in cognitive function and the rs11887120 SNP located in DNMT3A, a gene implicated in DNA methylation. Here, we ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2015-0014
更新日期:2016-05-01 00:00:00
abstract::Aim: The goal of this study was to comprehensively interrogate and map DNA methylation across 16 CpG-dense regions previously associated with oral and pharyngeal squamous cell carcinoma (OPSCC). Materials & methods: Targeted multiplex bisulfite amplicon sequencing was performed on four OPSCC cell lines and primary non...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2018-0172
更新日期:2019-07-01 00:00:00
abstract::Imprinted genes are an epigenetically regulated class of genes that are preferentially expressed from one parental allele. A number of these genes are crucial for placental function and embryonic growth in mice and humans. Disruption of imprinted genes is also associated with several neurodevelopmental disorders, alth...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.10.61
更新日期:2010-12-01 00:00:00
abstract::Hematopoiesis is a regulated multistep process, whereby transcriptional and epigenetic events contribute to progenitor fate determination. miRNAs have emerged as key players in hematopoietic cell development, differentiation and malignant transformation. From embryonic development through to adult life, miRNAs coopera...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi.13.55
更新日期:2013-01-01 00:00:00
abstract::For the first time, the development of next-generation sequencing technologies has brought about tools to investigate epigenetic alterations in an unbiased, yet genome-wide approach. The importance of this innovative technology is undeniable since it has already been established that changes in DNA methylation play an...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.09.50
更新日期:2010-04-01 00:00:00
abstract:AIM:This study aims to couple DNA methylation changes and evolution of retrogenes. MATERIALS & METHODS:A new two-step strategy was developed to screen retrogenes. Further, reduced representation bisulfite sequencing and RNA-seq data of eight tissues were used to analyze retrogenes. RESULTS:A total of 964 retrocopies ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2017-0071
更新日期:2018-02-01 00:00:00
abstract::Fundamental aspects of mammalian brain evolution occurred in the context of viviparity and placentation brought about by the epigenetic regulation of imprinted genes. Since the fetal placenta hormonally primes the maternal brain, two genomes in one individual are transgenerationally co-adapted to ensure maternal care ...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.11.10
更新日期:2011-04-01 00:00:00
abstract::Post-translational modifications of chromatin have become a 'booming' area of biomedical research. One particularly interesting modification that is important for eukaryotic gene expression is trimethylation of histone H3 lysine 4 (H3K4me3), which is almost exclusively associated with active promoters of RNA polymeras...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.10.11
更新日期:2010-06-01 00:00:00
abstract::Aim: Myotonic dystrophy type 1 (DM1) is caused by an unstable trinucleotide (CTG) expansion at the DMPK gene locus. Cognitive dysfunctions are often observed in the condition. We investigated the association between DMPK blood DNA methylation (DNAm) and cognitive functions in DM1, considering expansion length and vari...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0328
更新日期:2020-12-01 00:00:00
abstract::Aim: We aimed to explore the roles of noncoding RNAs (ncRNAs) in renal cell carcinoma. Materials & methods: The altered expressions of miR-196a2, miR-499a, H19, MALAT1 and GAS5, as well as some target transcripts were identified by quantitative real-time reverse transcription polymerase chain reaction. Results: Up-reg...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2019-0120
更新日期:2020-02-01 00:00:00
abstract:AIM:The aim was to investigate the effect of miR-377 on DNMT1 expression and cancer phenotype in pancreatic cancer cells. MATERIALS & METHODS:Real-time PCR, luciferase assay, MTT and Annexin-PI staining were used. RESULTS:Decreased miR-377 and increased DNMT1 (verified as a target for mir-377) levels in pancreatic ca...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0175
更新日期:2017-08-01 00:00:00
abstract::Aim: This study aims to examine the DNA methylation (DNAm) and expression patterns of genes associated with placental angiogenesis in preeclampsia. Materials & methods: DNAm and expression were examined in normotensive (n = 100) and preeclampsia (n = 100) women using pyrosequencing and quantitative real-time PCR respe...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0318
更新日期:2021-01-20 00:00:00
abstract::Idiopathic pulmonary fibrosis (IPF) is a complex lung disease of unknown etiology. Development of IPF is influenced by both genetic and environmental factors. Gene-expression profiling studies have taught us quite a bit about the biology of this fatal disease, but epigenetic marks may be the missing link that connects...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.12.10
更新日期:2012-04-01 00:00:00
abstract::Fibrosis is the excess deposition of extracellular matrix components which occur in multiple organs and ultimately leads to organ failure. Long noncoding RNAs (lncRNAs) are a kind of noncoding RNAs longer than approximately 200 nucleotides with no protein-encoding capacity. A growing body of evidence suggests that lnc...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi-2017-0020
更新日期:2017-09-01 00:00:00
abstract::Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used for mapping histone modifications, histone proteins, chromatin regulators, transcription factors and other DNA-binding proteins. It has played a significant role in our understanding of disease mechanisms and in exploring epigenetic changes...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi-2016-0053
更新日期:2016-09-01 00:00:00
abstract::Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnet...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0353
更新日期:2021-01-07 00:00:00
abstract:AIM:To characterize the genotypic and phenotypic extent of multilocus imprinting disturbances (MLID). MATERIALS & METHODS:We analyzed 37 patients with imprinting disorders (explorative cohort) for DNA methylation changes using the Infinium HumanMethylation450 BeadChip. For validation, three independent cohorts with im...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0007
更新日期:2016-06-01 00:00:00
abstract::Stephen B Baylin is a codirector of the Cancer Biology Program at The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins and the Virginia and DK Ludwig Professor of Oncology and Medicine. Baylin attended Duke University, where he earned his medical degree and completed his internship and first year residency i...
journal_title:Epigenomics
pub_type: 面试
doi:10.2217/epi.15.61
更新日期:2015-01-01 00:00:00