Abstract:
AIM:To identify regions of aberrant DNA methylation in acute lymphoblastic leukemia (ALL) cells of different subtypes on a genome-wide scale. MATERIALS & METHODS:Whole-genome bisulfite sequencing (WGBS) was used to determine the DNA methylation levels in cells from four pediatric ALL patients of different subtypes. The findings were confirmed by 450k DNA methylation arrays in a large patient set. RESULTS:Compared with mature B or T cells WGBS detected on average 82,000 differentially methylated regions per patient. Differentially methylated regions are enriched to CpG poor regions, active enhancers and transcriptional start sites. We also identified approximately 8000 CpG islands with variable intermediate DNA methylation that seems to occur as a result of stochastic de novo methylation. CONCLUSION:WGBS provides an unbiased view and novel insights into the DNA methylome of ALL cells.
journal_name
Epigenomicsjournal_title
Epigenomicsauthors
Wahlberg P,Lundmark A,Nordlund J,Busche S,Raine A,Tandre K,Rönnblom L,Sinnett D,Forestier E,Pastinen T,Lönnerholm G,Syvänen ACdoi
10.2217/epi-2016-0052subject
Has Abstractpub_date
2016-10-01 00:00:00pages
1367-1387issue
10eissn
1750-1911issn
1750-192Xjournal_volume
8pub_type
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