Abstract:
:In plants, pathogen triggered programmed cell death (PCD) is frequently mediated by polar lipid molecules referred as long chain bases (LCBs) or ceramides. PCD interceded by LCBs is a well-organized process where several cell organelles play important roles. In fact, light-dependent reactions in the chloroplast have been proposed as major players during PCD, however, the functional aspects of the chloroplast during PCD are largely unknown. For this reason, we investigated events that lead to disassembly of the chloroplast during PCD mediated by LCBs. To do so, LCB elevation was induced with Pseudomonas syringae pv. tomato (a non-host pathogen) or Fumonisin B1 in Phaseolus vulgaris. Then, we performed biochemical tests to detect PCD triggering events (phytosphingosine rises, MPK activation and H2O2 generation) followed by chloroplast structural and functional tests. Observations of the chloroplast, via optical phenotyping methods combined with microscopy, indicated that the loss of photosynthetic linear electron transport coincides with the organized ultrastructure disassembly. In addition, structural changes occurred in parallel with accumulation of H2O2 inside the chloroplast. These features revealed the collapse of chloroplast integrity and function as a mechanism leading to the irreversible execution of the PCD promoted by LCBs.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zavafer A,González-Solís A,Palacios-Bahena S,Saucedo-García M,Tapia de Aquino C,Vázquez-Santana S,King-Díaz B,Gavilanes-Ruiz Mdoi
10.1038/s41598-020-65186-8subject
Has Abstractpub_date
2020-06-25 00:00:00pages
10360issue
1issn
2045-2322pii
10.1038/s41598-020-65186-8journal_volume
10pub_type
杂志文章abstract::Jaw1/LRMP is a type II integral membrane protein that is localized at the endoplasmic reticulum (ER) and outer nuclear membrane. We previously reported that a function of Jaw1 is to maintain the nuclear shape as a KASH protein via its carboxyl terminal region, a component of linker of nucleoskeleton and cytoskeleton c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80258-5
更新日期:2021-01-12 00:00:00
abstract::Elastography plays a key role in characterizing soft media such as biological tissue. Although this technology has found widespread use in both clinical diagnostics and basic science research, nearly all methods require direct physical contact with the object of interest and can even be invasive. For a number of appli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38967
更新日期:2016-12-23 00:00:00
abstract::Predicting regions at risk from introductions of non-native species and the subsequent invasions is a fundamental aspect of horizon scanning activities that enable the development of more effective preventative actions and planning of management measures. The Asian cyprinid fish topmouth gudgeon Pseudorasbora parva ha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26316
更新日期:2016-05-20 00:00:00
abstract::Brain hypometabolism is a common epilepsy-related finding in both patients and animal models. Fluorodeoxyglucose positron emission tomography studies have shown that recurrent seizures lead to reduced glucose metabolism in certain brain regions, but no studies have definitively determined whether this induces epilepto...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05038-0
更新日期:2017-07-19 00:00:00
abstract::Synesthesia, an anomalous blending of senses in which stimulation of one sensory modality produces sensation in a different modality, provides a unique opportunity to study how multimodal information is represented in the human brain. We investigated how pitch classes (do, re, mi, etc.) are associated with the three d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18150-y
更新日期:2017-12-19 00:00:00
abstract::The chloride gradient plays an important role in regulating cell volume, membrane potential, pH, secretion, and the reversal potential of inhibitory glycine and GABAA receptors. Measurement of intracellular chloride activity, [Formula: see text], using liquid membrane ion-selective microelectrodes (ISM), however, has ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54885-6
更新日期:2019-12-11 00:00:00
abstract::Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength layer to close the vessel. He...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21681-7
更新日期:2018-02-19 00:00:00
abstract::Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29935
更新日期:2016-07-20 00:00:00
abstract::The controllability of a network is a theoretical problem of relevance in a variety of contexts ranging from financial markets to the brain. Until now, network controllability has been characterized only on isolated networks, while the vast majority of complex systems are formed by multilayer networks. Here we build a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20706
更新日期:2016-02-12 00:00:00
abstract::Wood density (WD) relates to important tree functions such as stem mechanics and resistance against pathogens. This functional trait can exhibit high intraindividual variability both radially and vertically. With the rise of LiDAR-based methodologies allowing nondestructive tree volume estimations, failing to account ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58733-w
更新日期:2020-02-06 00:00:00
abstract::Bone tissues have trabecular bone with a high bone turnover and cortical bone with a low turnover. The mechanisms by which the turnover rate of these bone tissues is determined remain unclear. Osteocytes secrete sclerostin, a Wnt/β-catenin signaling antagonist, and inhibit bone formation. We found that sclerostin expr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70817-1
更新日期:2020-08-13 00:00:00
abstract::To compare the performance of eight score systems (MELD, uMELD, MELD-Na. iMELD, UKELD, MELD-AS, CTP, and mCTP) in predicting the post-transplant mortality, we analyzed the data of 6,014 adult cirrhotic patients who underwent liver transplantation between January 2003 and December 2010 from the China Liver Transplant R...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42253
更新日期:2017-02-13 00:00:00
abstract::We present a new collusion attack to a kind of quantum secret sharing schemes with entangled states. Using this attack, an unauthorized set of agents can gain access to the shared secret without the others' cooperation. Furthermore, we establish a general model for this kind of quantum secret sharing schemes and then ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02543-0
更新日期:2017-05-30 00:00:00
abstract::The risk of suffering from Alzheimer's disease (AD) is higher in individuals from AD-affected mothers. The purpose of this investigation was to study whether maternal transmission might produce AD-related alterations in progenies of mice that do not have any genotypic alteration. We used cognitively-intact mothers har...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24710-7
更新日期:2018-04-24 00:00:00
abstract::Change in body composition with skeletal muscle wasting, a major component of pulmonary cachexia, is associated with mortality in chronic obstructive pulmonary disease and cancer. However, its relevance in interstitial lung diseases (ILDs) remains unclear. We hypothesized changes in body composition would be associate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32478-z
更新日期:2018-09-19 00:00:00
abstract::Cardiac tissue engineering using biomaterials with or without combination of stem cell therapy offers a new option for repairing infarcted heart. However, the bioactivity of biomaterials remains to be optimized because currently available biomaterials do not mimic the biochemical components as well as the structural p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39988
更新日期:2017-01-04 00:00:00
abstract::Catharanthus roseus is a commercial source for anti-cancer terpenoid indole alkaloids (TIAs: vincristine and vinblastine). Inherent levels of these TIAs are very low, hence research studies need to focus on enhancing their levels in planta. Since primary metabolism provides precursors for specialized-metabolism, eleva...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33415-w
更新日期:2018-10-10 00:00:00
abstract::Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12668-x
更新日期:2017-09-29 00:00:00
abstract::Sesame is prized for its oil. Genetic improvement of sesame can be enhanced through marker-assisted breeding. However, few simple sequence repeat (SSR) markers and SSR-based genetic maps were available in sesame. In this study, 7,357 SSR markers were developed from the sesame genome and transcriptomes, and a genetic m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08858-2
更新日期:2017-08-21 00:00:00
abstract::Electromagnetic resonances in conducting structures give rise to the enhancement of local fields and extinction efficiencies. Conducting structures are conventionally fabricated with a fixed geometry that determines their resonant response. Here, we challenge this conventional approach by demonstrating the photo-gener...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03584
更新日期:2014-01-07 00:00:00
abstract::We study an Ising chain undergoing a quantum phase transition in a quantum magnetic field. Such a field can be emulated by coupling the chain to a central spin initially in a superposition state. We show that--by adiabatically driving such a system--one can prepare a quantum superposition of any two ground states of t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00655
更新日期:2012-01-01 00:00:00
abstract::Hypoxic ischemic encephalopathy (HIE) is a major cause of neonatal mortality and morbidity. Our study sought to examine whether patterns of newborn screening analytes differed between infants with and without neonatal HIE in order to identify opportunities for potential use of these analytes for diagnosis in routine c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51919-x
更新日期:2019-10-31 00:00:00
abstract::Associations between cognitive decline and cerebral microbleeds (CMBs) have received increasing attention. An association between CMB distribution (deep or lobar) and cognitive decline has been reported, but these findings are controversial. We investigated the association between magnetic resonance imaging (MRI) find...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73404-6
更新日期:2020-10-02 00:00:00
abstract::Wooden shipwrecks are a significant part of the underwater cultural heritage. In 2007, the Nanhai No. 1 shipwreck was salvaged from the seabed and moved into the Marine Silk Road Museum, where it is still stored in a water tank. We analysed the microbial communities colonizing the hull surface of the Nanhai No. 1 ship...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25484-8
更新日期:2018-05-08 00:00:00
abstract::Antibody phage display has been pivotal in the quest to generate human monoclonal antibodies for biomedical and research applications. Target antigen preparation is a main bottleneck associated with the panning process. This includes production complexity, downstream purification, quality and yield. In many instances,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42628-6
更新日期:2019-04-15 00:00:00
abstract::Parkinson's Disease can be understood as a disorder of motor habits. A prediction of this theory is that early stage Parkinson's patients will display fewer errors caused by interference from previously over-learned behaviours. We test this prediction in the domain of skilled typing, where actions are easy to record a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39294-z
更新日期:2019-03-04 00:00:00
abstract::Neutrophil gelatinase-associated lipocalin (NGAL) is involved in cardiovascular and renal diseases. Gene inactivation of NGAL blunts the pathophysiological consequences of cardiovascular and renal damage. We aimed to design chemical NGAL inhibitors and investigate its effects in experimental models of myocardial infar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-82279-0
更新日期:2021-01-28 00:00:00
abstract::Mitochondria are organelles that govern energy supply and control cell death. Mitochondria also express bacterial features, such as the presence of inner membrane cardiolipin and a circular genome rich in hypomethylated CpG motifs. While mitochondrial extrusion by damaged organs or activated cells is thought to trigge...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40900-3
更新日期:2019-03-14 00:00:00
abstract::Human children show unique cognitive skills for dealing with the social world but their cognitive performance is paralleled by great apes in many tasks dealing with the physical world. Recent studies suggested that members of a songbird family-corvids-also evolved complex cognitive skills but a detailed understanding ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77060-8
更新日期:2020-12-10 00:00:00
abstract::To elude the strength-electrical conductivity trade-off dilemma, a nanostructuring strategy was achieved in microalloyed Al-0.1wt.% Zr conductor by optimizing the processing route, leading to enhanced strength and simultaneously improved electrical conductivity. The nanostructural design involved ultrafine grains with...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24527-4
更新日期:2018-04-18 00:00:00