Organized Disassembly of Photosynthesis During Programmed Cell Death Mediated By Long Chain Bases.

Abstract:

:In plants, pathogen triggered programmed cell death (PCD) is frequently mediated by polar lipid molecules referred as long chain bases (LCBs) or ceramides. PCD interceded by LCBs is a well-organized process where several cell organelles play important roles. In fact, light-dependent reactions in the chloroplast have been proposed as major players during PCD, however, the functional aspects of the chloroplast during PCD are largely unknown. For this reason, we investigated events that lead to disassembly of the chloroplast during PCD mediated by LCBs. To do so, LCB elevation was induced with Pseudomonas syringae pv. tomato (a non-host pathogen) or Fumonisin B1 in Phaseolus vulgaris. Then, we performed biochemical tests to detect PCD triggering events (phytosphingosine rises, MPK activation and H2O2 generation) followed by chloroplast structural and functional tests. Observations of the chloroplast, via optical phenotyping methods combined with microscopy, indicated that the loss of photosynthetic linear electron transport coincides with the organized ultrastructure disassembly. In addition, structural changes occurred in parallel with accumulation of H2O2 inside the chloroplast. These features revealed the collapse of chloroplast integrity and function as a mechanism leading to the irreversible execution of the PCD promoted by LCBs.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Zavafer A,González-Solís A,Palacios-Bahena S,Saucedo-García M,Tapia de Aquino C,Vázquez-Santana S,King-Díaz B,Gavilanes-Ruiz M

doi

10.1038/s41598-020-65186-8

subject

Has Abstract

pub_date

2020-06-25 00:00:00

pages

10360

issue

1

issn

2045-2322

pii

10.1038/s41598-020-65186-8

journal_volume

10

pub_type

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