Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus.

Abstract:

:Catharanthus roseus is a commercial source for anti-cancer terpenoid indole alkaloids (TIAs: vincristine and vinblastine). Inherent levels of these TIAs are very low, hence research studies need to focus on enhancing their levels in planta. Since primary metabolism provides precursors for specialized-metabolism, elevating the former can achieve higher amounts of the latter. Cell Wall Invertase (CWIN), a key enzyme in sucrose-metabolism catalyses the breakdown of sucrose into glucose and fructose, which serve as carbon-skeleton for specialized-metabolites. Understanding CWIN regulation could unravel metabolic-engineering approaches towards enhancing the levels of TIAs in planta. Our study is the first to characterize CWIN at gene-expression level in the medicinal plant, C. roseus. The CWINs and their inter-relationship with sucrose and TIA metabolism was studied at gene and metabolite levels. It was found that sucrose-supplementation to C. roseus leaves significantly elevated the monomeric TIAs (vindoline, catharanthine) and their corresponding genes. This was further confirmed in cross-species, wherein Nicotiana benthamiana leaves transiently-overexpressing CrCWIN2 showed significant upregulation of specialized-metabolism genes: NbPAL2, Nb4CL, NbCHS, NbF3H, NbANS, NbHCT and NbG10H. The specialized metabolites- cinnamic acid, coumarin, and fisetin were significantly upregulated. Thus, the present study provides a valuable insight into metabolic-engineering approaches towards augmenting the levels of therapeutic TIAs.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Nishanth MJ,Sheshadri SA,Rathore SS,Srinidhi S,Simon B

doi

10.1038/s41598-018-33415-w

subject

Has Abstract

pub_date

2018-10-10 00:00:00

pages

15059

issue

1

issn

2045-2322

pii

10.1038/s41598-018-33415-w

journal_volume

8

pub_type

杂志文章
  • An evaluation of the accuracy and speed of metagenome analysis tools.

    abstract::Metagenome studies are becoming increasingly widespread, yielding important insights into microbial communities covering diverse environments from terrestrial and aquatic ecosystems to human skin and gut. With the advent of high-throughput sequencing platforms, the use of large scale shotgun sequencing approaches is n...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19233

    authors: Lindgreen S,Adair KL,Gardner PP

    更新日期:2016-01-18 00:00:00

  • Transcriptome profiles of hypothalamus and adrenal gland linked to haplotype related to coping behavior in pigs.

    abstract::The hypothalamic-pituitary-adrenal (HPA) axis is an important component of neuroendocrine stress regulation and coping behavior. Transcriptome profiles of the hypothalamus and adrenal gland were assessed to identify molecular pathways and candidate genes for coping behavior in pigs. Ten each of high- (HR) and low- (LR...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49521-2

    authors: Gley K,Murani E,Trakooljul N,Zebunke M,Puppe B,Wimmers K,Ponsuksili S

    更新日期:2019-09-10 00:00:00

  • Mutational analysis of TSC1 and TSC2 genes in Tuberous Sclerosis Complex patients from Greece.

    abstract::Tuberous sclerosis complex (TSC) is a rare autosomal dominant disorder causing benign tumors in the brain and other vital organs. The genes implicated in disease development are TSC1 and TSC2. Here, we have performed mutational analysis followed by a genotype-phenotype correlation study based on the clinical character...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16988-w

    authors: Avgeris S,Fostira F,Vagena A,Ninios Y,Delimitsou A,Vodicka R,Vrtel R,Youroukos S,Stravopodis DJ,Vlassi M,Astrinidis A,Yannoukakos D,Voutsinas GE

    更新日期:2017-12-01 00:00:00

  • Differential electron emission from polycyclic aromatic hydrocarbon molecules under fast ion impact.

    abstract::Interaction between polycyclic aromatic hydrocarbon (PAH) molecule and energetic ion is a subject of interest in different areas of modern physics. Here, we present measurements of energy and angular distributions of absolute double differential electron emission cross section for coronene (C24H12) and fluorene (C13H1...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05149-8

    authors: Biswas S,Champion C,Weck PF,Tribedi LC

    更新日期:2017-07-17 00:00:00

  • Multiple dimensions of biodiversity drive human interest in tide pool communities.

    abstract::Activities involving observation of wild organisms (e.g. wildlife watching, tidepooling) can provide recreational and learning opportunities, with biologically diverse animal assemblages expected to be more stimulating to humans. In turn, more diverse communities may enhance human interest and facilitate provisioning ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-33155-x

    authors: Fairchild TP,Fowler MS,Pahl S,Griffin JN

    更新日期:2018-10-15 00:00:00

  • Foliar fungi of Betula pendula: impact of tree species mixtures and assessment methods.

    abstract::Foliar fungi of silver birch (Betula pendula) in an experimental Finnish forest were investigated across a gradient of tree species richness using molecular high-throughput sequencing and visual macroscopic assessment. We hypothesized that the molecular approach detects more fungal taxa than visual assessment, and tha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41801

    authors: Nguyen D,Boberg J,Cleary M,Bruelheide H,Hönig L,Koricheva J,Stenlid J

    更新日期:2017-02-02 00:00:00

  • Optogenetic activation of axon guidance receptors controls direction of neurite outgrowth.

    abstract::Growth cones of extending axons navigate to correct targets by sensing a guidance cue gradient via membrane protein receptors. Although most signaling mechanisms have been clarified using an in vitro approach, it is still difficult to investigate the growth cone behavior in complicated extracellular environment of liv...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23976

    authors: Endo M,Hattori M,Toriyabe H,Ohno H,Kamiguchi H,Iino Y,Ozawa T

    更新日期:2016-04-07 00:00:00

  • Life cycle assessment of polyphenols extraction processes from waste biomass.

    abstract::Waste biomass from forestry and wood processing industries is a source to obtain fine chemicals, and its processing is a good example of circular economy, but it generates secondary environmental impacts. The main objective of this study was to analyse the environmental performances of laboratory scale processes for p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70587-w

    authors: Barjoveanu G,Pătrăuțanu OA,Teodosiu C,Volf I

    更新日期:2020-08-12 00:00:00

  • A structural-chemical explanation of fungal laccase activity.

    abstract::Fungal laccases (EC 1.10.3.2) are multi-copper oxidases that oxidize a wide variety of substrates. Despite extensive studies, the molecular basis for their diverse activity is unclear. Notably, there is no current way to rationally predict the activity of a laccase toward a given substrate. Such knowledge would greatl...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35633-8

    authors: Mehra R,Muschiol J,Meyer AS,Kepp KP

    更新日期:2018-11-23 00:00:00

  • Studying the role of axon fasciculation during development in a computational model of the Xenopus tadpole spinal cord.

    abstract::During nervous system development growing axons can interact with each other, for example by adhering together in order to produce bundles (fasciculation). How does such axon-axon interaction affect the resulting axonal trajectories, and what are the possible benefits of this process in terms of network function? In t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-13804-3

    authors: Davis O,Merrison-Hort R,Soffe SR,Borisyuk R

    更新日期:2017-10-19 00:00:00

  • Modeling dynamic interactions between pre-exposure prophylaxis interventions & treatment programs: predicting HIV transmission & resistance.

    abstract::Clinical trials have recently demonstrated the effectiveness of Pre-Exposure Prophylaxis (PrEP) in preventing HIV infection. Consequently, PrEP may soon be used for epidemic control. We model the dynamic interactions that will occur between treatment programs and potential PrEP interventions in resource-constrained co...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00185

    authors: Supervie V,Barrett M,Kahn JS,Musuka G,Moeti TL,Busang L,Blower S

    更新日期:2011-01-01 00:00:00

  • Fermi arcs vs. Fermi pockets in electron-doped perovskite iridates.

    abstract::We report on an angle resolved photoemission (ARPES) study of bulk electron-doped perovskite iridate, (Sr(1-x)La(x))3Ir2O7. Fermi surface pockets are observed with a total electron count in keeping with that expected from La substitution. Depending on the energy and polarization of the incident photons, these pockets ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08533

    authors: He J,Hafiz H,Mion TR,Hogan T,Dhital C,Chen X,Lin Q,Hashimoto M,Lu DH,Zhang Y,Markiewicz RS,Bansil A,Wilson SD,He RH

    更新日期:2015-02-23 00:00:00

  • Earliest known lepisosteoid extends the range of anatomically modern gars to the Late Jurassic.

    abstract::Lepisosteoids are known for their evolutionary conservatism, and their body plan can be traced at least as far back as the Early Cretaceous, by which point two families had diverged: Lepisosteidae, known since the Late Cretaceous and including all living species and various fossils from all continents, except Antarcti...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17984-w

    authors: Brito PM,Alvarado-Ortega J,Meunier FJ

    更新日期:2017-12-19 00:00:00

  • Digit ratio (2D:4D) and transgender identity: new original data and a meta-analysis.

    abstract::Previously reported associations between second-to-fourth digit length ratio (2D:4D), a proxy for prenatal androgen load, and transgender identity have been inconsistent. The objectives of the present study were to provide additional original data and an updated meta-analysis concerning this association. In a study of...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72486-6

    authors: Siegmann EM,Müller T,Dziadeck I,Mühle C,Lenz B,Kornhuber J

    更新日期:2020-11-09 00:00:00

  • Scaling and correlation properties of RR and QT intervals at the cellular level.

    abstract::We study complex scaling properties of RR and QT intervals of electrocardiograms (ECGs) with their equivalences at the cellular level, that is, inter-beat intervals (IBI) and field potential durations (FPD) of spontaneously beating human-induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) aggregates. Our de...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40247-9

    authors: Kim J,Shah D,Potapov I,Latukka J,Aalto-Setälä K,Räsänen E

    更新日期:2019-03-06 00:00:00

  • Somatostatin triggers rhythmic electrical firing in hypothalamic GHRH neurons.

    abstract::Hypothalamic growth hormone-releasing hormone (GHRH) neurons orchestrate body growth/maturation and have been implicated in feeding responses and ageing. However, the electrical patterns that dictate GHRH neuron functions have remained elusive. Since the inhibitory neuropeptide somatostatin (SST) is considered to be a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24394

    authors: Osterstock G,Mitutsova V,Barre A,Granier M,Fontanaud P,Chazalon M,Carmignac D,Robinson IC,Low MJ,Plesnila N,Hodson DJ,Mollard P,Méry PF

    更新日期:2016-04-13 00:00:00

  • Grain Boundary Specific Segregation in Nanocrystalline Fe(Cr).

    abstract::A cross-correlative precession electron diffraction - atom probe tomography investigation of Cr segregation in a Fe(Cr) nanocrystalline alloy was undertaken. Solute segregation was found to be dependent on grain boundary type. The results of which were compared to a hybrid Molecular Dynamics and Monte Carlo simulation...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34642

    authors: Zhou X,Yu XX,Kaub T,Martens RL,Thompson GB

    更新日期:2016-10-06 00:00:00

  • Controlling evolution of protein corona: a prosperous approach to improve chitosan-based nanoparticle biodistribution and half-life.

    abstract::Protein corona significantly affects in vivo fate of nanoparticles including biodistribution and half-life. Without manipulating the physicochemical properties of nanoparticles with considering their biointerference, attaining effective treatment protocols is impossible. For this reason, protein corona evolution and b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-66572-y

    authors: Tekie FSM,Hajiramezanali M,Geramifar P,Raoufi M,Dinarvand R,Soleimani M,Atyabi F

    更新日期:2020-06-15 00:00:00

  • AlN formation by an Al/GaN substitution reaction.

    abstract::Aluminium nitride (AlN) is a promising semiconductor material for use as a substrate in high-power, high-frequency electronic and deep-ultraviolet optoelectronic devices. We study the feasibility of a novel AlN fabrication technique by using the Al/GaN substitution reaction method. The substitution method we propose h...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69992-y

    authors: Noorprajuda M,Ohtsuka M,Adachi M,Fukuyama H

    更新日期:2020-08-03 00:00:00

  • Injection-modulated polarity conversion by charge carrier density control via a self-assembled monolayer for all-solution-processed organic field-effect transistors.

    abstract::We demonstrated modulation of charge carrier densities in all-solution-processed organic field-effect transistors (OFETs) by modifying the injection properties with self-assembled monolayers (SAMs). The all-solution-processed OFETs based on an n-type polymer with inkjet-printed Ag electrodes were fabricated as a test ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep46365

    authors: Roh J,Lee T,Kang CM,Kwak J,Lang P,Horowitz G,Kim H,Lee C

    更新日期:2017-04-12 00:00:00

  • Effects of short-term heat shock and physiological responses to heat stress in two Bradysia adults, Bradysia odoriphaga and Bradysia difformis.

    abstract::Bradysia odoriphaga and Bradysia difformis are devastating pests of vegetable, ornamental crops and edible mushrooms causing significant losses. Temperature may be an important factor restricting their population abundance in the summer. To determine the effects of short-term heat shock on adults, their survival, long...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-13560-4

    authors: Zhu G,Xue M,Luo Y,Ji G,Liu F,Zhao H,Sun X

    更新日期:2017-10-17 00:00:00

  • Engineering of soybean mosaic virus as a versatile tool for studying protein-protein interactions in soybean.

    abstract::Transient gene expression approaches are valuable tools for rapid introduction of genes of interest and characterization of their functions in plants. Although agroinfiltration is the most effectively and routinely used method for transient expression of multiple genes in various plant species, this approach has been ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22436

    authors: Seo JK,Choi HS,Kim KH

    更新日期:2016-02-29 00:00:00

  • The estrogenicity of methylparaben and ethylparaben at doses close to the acceptable daily intake in immature Sprague-Dawley rats.

    abstract::The estrogenicity of parabens at human exposure levels has become a focus of concern due to the debate over whether the estrogenicity of parabens is strong enough to play a role in the increased incidence of breast cancer. In this study, the uterotrophic activities of methylparaben (MP) and ethylparaben (EP) at doses ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep25173

    authors: Sun L,Yu T,Guo J,Zhang Z,Hu Y,Xiao X,Sun Y,Xiao H,Li J,Zhu D,Sai L,Li J

    更新日期:2016-04-28 00:00:00

  • SOX7 co-regulates Wnt/β-catenin signaling with Axin-2: both expressed at low levels in breast cancer.

    abstract::SOX7 as a tumor suppressor belongs to the SOX F gene subfamily and is associated with a variety of human cancers, including breast cancer, but the mechanisms involved are largely unclear. In the current study, we investigated the interactions between SOX7 and AXIN2 in their co-regulation on the Wnt/β-catenin signal pa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26136

    authors: Liu H,Mastriani E,Yan ZQ,Yin SY,Zeng Z,Wang H,Li QH,Liu HY,Wang X,Bao HX,Zhou YJ,Kou JJ,Li D,Li T,Liu J,Liu Y,Yin L,Qiu L,Gong L,Liu SL

    更新日期:2016-05-18 00:00:00

  • Morphology control of exciton fine structure in polar and nonpolar zinc sulfide nanorods.

    abstract::Electron-hole exchange interaction in semiconductor quantum dots (QDs) splits the band-edge exciton manifold into optically active ("bright") and passive ("dark") states, leading to a complicated exciton fine structure. In the present work, we resolve by atomistic million-atom many-body pseudopotential calculations th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09812-y

    authors: Baskoutas S,Zeng Z,Garoufalis CS,Bester G

    更新日期:2017-08-24 00:00:00

  • Conserved interactions required for inhibition of the main protease of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

    abstract::The COVID-19 pandemic caused by the SARS-CoV-2 requires a fast development of antiviral drugs. SARS-CoV-2 viral main protease (Mpro, also called 3C-like protease, 3CLpro) is a potential target for drug design. Crystal and co-crystal structures of the SARS-CoV-2 Mpro have been solved, enabling the rational design of in...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77794-5

    authors: Shitrit A,Zaidman D,Kalid O,Bloch I,Doron D,Yarnizky T,Buch I,Segev I,Ben-Zeev E,Segev E,Kobiler O

    更新日期:2020-11-30 00:00:00

  • Long term outcomes in survivors of epidemic Influenza A (H7N9) virus infection.

    abstract::Patients who survive influenza A (H7N9) virus infection are at risk of physical and psychological complications of lung injury and multi-organ dysfunction. However, there were no prospectively individualized assessments of physiological, functional and quality-of-life measures after hospital discharge. The current stu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17497-6

    authors: Chen J,Wu J,Hao S,Yang M,Lu X,Chen X,Li L

    更新日期:2017-12-08 00:00:00

  • Tuneable quantum spin Hall states in confined 1T' transition metal dichalcogenides.

    abstract::Investigation of quantum spin Hall states in 1T' phase of the monolayer transition metal dichalcogenides has recently attracted the attention for its potential in nanoelectronic applications. While most of the theoretical findings in this regard deal with infinitely periodic crystal structures, here we employ density ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63450-5

    authors: Das B,Sen D,Mahapatra S

    更新日期:2020-04-21 00:00:00

  • Bark and wood tissues of American elm exhibit distinct responses to Dutch elm disease.

    abstract::Tolerance to Dutch elm disease (DED) has been linked to the rapid and/or high induction of disease-responsive genes after infection with the fungus Ophiostoma novo-ulmi. Although the fungal infection by O. novo-ulmi primarily takes places in xylem vessels, it is still unclear how xylem contributes to the defense again...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07779-4

    authors: Sherif SM,Erland LA,Shukla MR,Saxena PK

    更新日期:2017-08-02 00:00:00

  • Early alterations in a mouse model of Rett syndrome: the GABA developmental shift is abolished at birth.

    abstract::Genetic mutations of the Methyl-CpG-binding protein-2 (MECP2) gene underlie Rett syndrome (RTT). Developmental processes are often considered to be irrelevant in RTT pathogenesis but neuronal activity at birth has not been recorded. We report that the GABA developmental shift at birth is abolished in CA3 pyramidal neu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-45635-9

    authors: Lozovaya N,Nardou R,Tyzio R,Chiesa M,Pons-Bennaceur A,Eftekhari S,Bui TT,Billon-Grand M,Rasero J,Bonifazi P,Guimond D,Gaiarsa JL,Ferrari DC,Ben-Ari Y

    更新日期:2019-06-25 00:00:00