Abstract：
:The chloride gradient plays an important role in regulating cell volume, membrane potential, pH, secretion, and the reversal potential of inhibitory glycine and GABAA receptors. Measurement of intracellular chloride activity, [Formula: see text], using liquid membrane ion-selective microelectrodes (ISM), however, has been limited by the physiochemical properties of Cl- ionophores which have caused poor stability, drift, sluggish response times, and interference from other biologically relevant anions. Most importantly, intracellular [Formula: see text] may be up to 4 times more abundant than Cl- (e.g. skeletal muscle) which places severe constraints on the required selectivity of a Cl- - sensing ISM. Previously, a sensitive and highly-selective Cl- sensor was developed in a polymeric membrane electrode using a trinuclear Hg(II) complex containing carborane-based ligands, [9]-mercuracarborand-3, or MC3 for short. Here, we have adapted the use of the MC3 anion carrier in a liquid membrane ion-selective microelectrode and show the MC3-ISM has a linear Nernstian response over a wide range of aCl (0.1 mM to 100 mM), is highly selective for Cl- over other biological anions or inhibitors of Cl- transport, and has a 10% to 90% settling time of 3 sec. Importantly, over the physiological range of aCl (1 mM to 100 mM) the potentiometric response of the MC3-ISM is insensitive to [Formula: see text] or changes in pH. Finally, we demonstrate the biological application of an MC3-ISM by measuring intracellular aCl, and the response to an external Cl-free challenge, for an isolated skeletal muscle fiber.
journal_name
Sci Repjournal_title
Scientific reportsauthors
DiFranco M,Quinonez M,Dziedzic RM,Spokoyny AM,Cannon SCdoi
10.1038/s41598-019-54885-6subject
Has Abstractpub_date
2019-12-11 00:00:00pages
18860issue
1issn
2045-2322pii
10.1038/s41598-019-54885-6journal_volume
9pub_type
杂志文章abstract：:Non-Markovianity has recently attracted large interest due to significant advances in its characterization and its exploitation for quantum information processing. However, up to now, only non-Markovian regimes featuring environment to system backflow of information (strong non-Markovianity) have been experimentally s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17520
更新日期：2015-12-02 00:00:00
abstract：:Centromeres usually contain large amounts of tandem repeat DNA. Alpha satellite DNA (AS) is the most abundant tandem repeat DNA found in the centromeres of simian primates. The AS of humans contains sequences organized into higher-order repeat (HOR) structures, which are tandem arrays of larger repeat units consisting...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10315
更新日期：2015-05-14 00:00:00
abstract：:Mosquito larvae continuously encounter microbes in their aquatic environment, which serve as food and play a critical role in successful development. In previous work, we isolated a Chromobacterium sp. (C.sp_P) with larvicidal activity from the midgut of dengue vector Aedes mosquitoes in Panama. In this study, we foun...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26680-2
更新日期：2018-05-29 00:00:00
abstract：:Observations and modeling studies have shown that during CO2 injection into underground carbonate reservoirs, the dissolution of CO2 into formation water forms acidic brine, leading to fluid-rock interactions that can significantly impact the hydraulic properties of the host formation. However, the impacts of these in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60247-4
更新日期：2020-02-27 00:00:00
abstract：:Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca(2+) imaging, and electrophys...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25657
更新日期：2016-05-10 00:00:00
abstract：:Concerns related to pulmonary toxicity and non-specificity of nanoparticles have limited their clinical applications for aerosol delivery of chemotherapeutics in lung cancer. We hypothesized that pulmonary surfactant mimetic nanoparticles that offer pH responsive release specifically in tumor may be a possible solutio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07085
更新日期：2014-11-18 00:00:00
abstract：:Temperature-modulated space-charge-limited-current spectroscopy (TMSCLC) is applied to quantitatively evaluate the density of trap states in the band-gap with high energy resolution of semiconducting hybrid lead halide perovskite single crystals. Interestingly multicomponent deep trap states were observed in the pure ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40139-y
更新日期：2019-03-04 00:00:00
abstract：:The white spot syndrome virus (WSSV) causes mass mortalities in the aquaculture of shrimps worldwide. The mud shrimp Austinogebia edulis (Ngoc-Ho & Chan, 1992) is an economically important sea food item occurring along the west coast of Taiwan. While the population of A. edulis began to decrease with some fluctuations...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54837-0
更新日期：2019-12-09 00:00:00
abstract：:Transjugular intrahepatic portosystemic shunt (TIPS) is an effective therapy for reducing portal pressure. Hepatic myelopathy (HM), a rare complication of chronic liver diseases, remains obscure in terms of treatment and prognosis. We aimed to determine an optimal treat strategy for patients with HM after TIPS. Twenty...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/s41598-018-33216-1
更新日期：2018-10-12 00:00:00
abstract：:Rotaviruses (RVs) are the most common cause of severe diarrheal disease. To date two rotavirus oral vaccines are licensed: Rotarix and Rotateq. Our aim was to contribute to the post-marketing evaluation of these vaccines safety profile. We collected all RV vaccines-related reports of Adverse Events Following Immunizat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70653-3
更新日期：2020-08-12 00:00:00
abstract：:Bacterial NhaB Na+/H+ exchangers belonging to the Ion Transporter superfamily are poorly characterized in contrast to Na+/H+ exchangers of the Cation Proton Antiporter superfamily which have NhaA from Escherichia coli as a prominent member. For a more detailed understanding of the intricacies of the exchanger's transp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51887-2
更新日期：2019-10-28 00:00:00
abstract：:Granule cells at the input layer of the cerebellum comprise over half the neurons in the human brain and are thought to be critical for learning. However, little is known about granule neuron signaling at the population scale during behavior. We used calcium imaging in awake zebrafish during optokinetic behavior to re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15938-w
更新日期：2017-11-24 00:00:00
abstract：:Visible indication based on the aggregation of colloidal nanoparticles (NPs) is highly advantageous for rapid on-site detection of biological entities, which even untrained persons can perform without specialized instrumentation. However, since the extent of aggregation should exceed a certain minimum threshold to pro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00456
更新日期：2012-01-01 00:00:00
abstract：:Several studies have advanced the idea that the etiology of Alzheimer's disease (AD) could be microbial in origin. In the present study, we tested the possibility that polymicrobial infections exist in tissue from the entorhinal cortex/hippocampus region of patients with AD using immunohistochemistry (confocal laser s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05903-y
更新日期：2017-07-17 00:00:00
abstract：:Cross-linked protein crystals (CLPCs) are very useful materials in applications such as biosensors, catalysis, and X-ray crystallography. Hence, preparation of CLPCs is an important research direction. During the preparation of CLPCs, an often encountered problem is that cracks may appear in the crystals, which may fi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34770
更新日期：2016-10-05 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-42889-1
更新日期：2019-06-10 00:00:00
abstract：:Extraction of mouse spinal motor neurons from transgenic mouse embryos recapitulating some aspects of neurodegenerative diseases like amyotrophic lateral sclerosis has met with limited success. Furthermore, extraction and long-term culture of adult mouse spinal motor neurons and glia remain also challenging. We presen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16763
更新日期：2015-11-18 00:00:00
abstract：:Stable electroluminescence from micro-pixelated light-emitting diode (μLED) occurs when electrons and holes are continuously injected from external electrodes. Different from the general recognition, in this work, μLED works in an operation mode, namely, non-electrical contact and non-carrier injection mode, and can b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65092-z
更新日期：2020-05-15 00:00:00
abstract：:The quantities and proportions of protein fractions have notable effects on the nutritional and technological value of milk. Although much is known about the effects of genetic variants on milk proteins, the complex relationships among the set of genes and pathways regulating the different protein fractions synthesis ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18916-4
更新日期：2018-01-12 00:00:00
abstract：:A tribochemistry-assisted method has been developed for nondestructive surface nanofabrication on GaAs. Without any applied electric field and post etching, hollow nanostructures can be directly fabricated on GaAs surfaces by sliding a SiO2 microsphere under an ultralow contact pressure in humid air. TEM observation o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09020
更新日期：2015-03-12 00:00:00
abstract：:Body mass index (BMI) is associated with increased future risk of inflammatory bowel disease(IBD) particularly Crohn's disease(CD), where associations with high and low BMI have been observed. Most studies are based on adult women. We aimed to explore the impact of BMI in men entering adult life on their long-term ris...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42642-8
更新日期：2019-04-23 00:00:00
abstract：:The potential energy profile of an ideal binary switch is a symmetric double well. Switching between the wells without energy dissipation requires time-modulating the height of the potential barrier separating the wells and tilting the profile towards the desired well at the precise juncture when the barrier disappear...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03204
更新日期：2013-11-13 00:00:00
abstract：:Somatic mutations of many cancer genes tend to co-occur (termed co-mutations) in certain patterns during tumor initiation and progression. However, the genetic and epigenetic mechanisms that contribute to the co-mutations of these cancer genes have yet to be explored. Here, we systematically investigated the associati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35270
更新日期：2016-10-20 00:00:00
abstract：:Emerging infectious diseases such as chytridiomycosis and ranavirus infections are important contributors to the worldwide decline of amphibian populations. We reviewed data on 247 anuran mortality events in 43 States of the United States from 1999-2015. Our findings suggest that a severe infectious disease of tadpole...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10456-1
更新日期：2017-08-31 00:00:00
abstract：:The majority of individuals with Down syndrome (DS) do not exhibit accurate accommodation, with the aetiology of this deficit unknown. This study examines the mechanism underlying hypoaccommodation in DS by simultaneously investigating the 'near triad' - accommodation, vergence and pupillary response. An objective pho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20444
更新日期：2016-02-05 00:00:00
abstract：:Limbal stem cell deficiency can be treated with transplantation of cultured human limbal epithelial cells (LEC). It can be advantageous to produce LEC in centralized labs and thereafter ship them to eye clinics. The present study used transport simulations of LEC to determine if vigorous shaking during transport alter...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28553-0
更新日期：2018-07-12 00:00:00
abstract：:A common philosophy in control theory is the control of disorder by order. Control of decoherence is no exception; strategies aimed at suppressing quantum decoherence adopt this point of view. Here we predict an anomalous phenomenon in open quantum systems-control of disorder by (even more) disorder. It is shown that ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02746
更新日期：2013-09-25 00:00:00
abstract：:Atomoxetine is a norepinephrine reuptake inhibitor indicated in the treatment of attention-deficit/hyperactivity disorder. It is primarily metabolized by CYP2D6 to its equipotent metabolite, 4-hydroxyatomoxetine, which promptly undergoes further glucuronidation to an inactive 4-HAT-O-glucuronide. Clinical trials have ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30841-8
更新日期：2018-08-17 00:00:00
abstract：:Invariant natural killer T (iNKT) cells are a specialized subset of T cells contributing to both, the innate and adaptive immune responses. In contrast to conventional T lymphocytes they recognize lipid antigens. The aim of the project is to establish a novel model system, to study iNKT-TCR - ligand interaction. An iN...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43529-4
更新日期：2019-05-15 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-37593-5
更新日期：2018-12-21 00:00:00
