Abstract：
:During cell division, remodelling of the nuclear envelope enables chromosome segregation by the mitotic spindle1. The reformation of sealed nuclei requires ESCRTs (endosomal sorting complexes required for transport) and LEM2, a transmembrane ESCRT adaptor2-4. Here we show how the ability of LEM2 to condense on microtubules governs the activation of ESCRTs and coordinated spindle disassembly. The LEM motif of LEM2 binds BAF, conferring on LEM2 an affinity for chromatin5,6, while an adjacent low-complexity domain (LCD) promotes LEM2 phase separation. A proline-arginine-rich sequence within the LCD binds to microtubules and targets condensation of LEM2 to spindle microtubules that traverse the nascent nuclear envelope. Furthermore, the winged-helix domain of LEM2 activates the ESCRT-II/ESCRT-III hybrid protein CHMP7 to form co-oligomeric rings. Disruption of these events in human cells prevented the recruitment of downstream ESCRTs, compromised spindle disassembly, and led to defects in nuclear integrity and DNA damage. We propose that during nuclear reassembly LEM2 condenses into a liquid-like phase and coassembles with CHMP7 to form a macromolecular O-ring seal at the confluence between membranes, chromatin and the spindle. The properties of LEM2 described here, and the homologous architectures of related inner nuclear membrane proteins7,8, suggest that phase separation may contribute to other critical envelope functions, including interphase repair8-13 and chromatin organization14-17.
journal_name
Naturejournal_title
Natureauthors
von Appen A,LaJoie D,Johnson IE,Trnka MJ,Pick SM,Burlingame AL,Ullman KS,Frost Adoi
10.1038/s41586-020-2232-xsubject
Has Abstractpub_date
2020-06-01 00:00:00pages
115-118issue
7810eissn
0028-0836issn
1476-4687pii
10.1038/s41586-020-2232-xjournal_volume
582pub_type
杂志文章相关文献
NATURE文献大全abstract：:The fundus of the guinea-pig stomach actively dilates in response to low increases in intragastric pressure. This physiological response, now called adaptive relaxation, accommodates the intake of liquid or food. It is independent of external innervation, resistant to ganglion blockade, but reflex in origin. The nerve...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/351477a0
更新日期：1991-06-06 00:00:00
abstract：:Lecithin is a major structural component of biological membranes. Because of their amphipathic nature, lecithin and related phospholipids tend to aggregate as bilayer structures in which the hydrophilic head groups are orientated towards the surface and the hydrophobic hydrocarbon chains towards the interior. A detail...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/281499a0
更新日期：1979-10-11 00:00:00
abstract：:Two main forms of long-term potentiation (LTP)-a prominent model for the cellular mechanism of learning and memory-have been distinguished in the mammalian brain. One requires activation of postsynaptic NMDA (N-methyl d-aspartate) receptors, whereas the other, called mossy fibre LTP, has a principal presynaptic compon...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/415327a
更新日期：2002-01-17 00:00:00
abstract：:Vaccines against a variety of infectious diseases represent one of the great triumphs of medicine. The immune correlates of protection induced by most current vaccines seem to be mediated by long-lived humoral immune responses. By contrast, there are no currently available vaccines that are uniformly effective for dis...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35021239
更新日期：2000-08-17 00:00:00
abstract：:Metabolic phenotypes are the products of interactions among a variety of factors-dietary, other lifestyle/environmental, gut microbial and genetic. We use a large-scale exploratory analytical approach to investigate metabolic phenotype variation across and within four human populations, based on 1H NMR spectroscopy. M...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature06882
更新日期：2008-05-15 00:00:00
abstract：:One approach to the controlled release of drugs involves incorporation of the drug molecules into the matrix of microscopic polymer spheres or capsules. Existing methods for preparing such microparticles do not, however, always guarantee a constant release rate, for example because drug molecules may be trapped prefer...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/367258a0
更新日期：1994-01-20 00:00:00
abstract：:The promoters of eukaryotic genes are being increasingly defined through the identification of consensus DNA sequences, by mutational analysis, and by in vitro and in vivo studies of transcription. Whereas the TATA sequence (Goldberg-Hogness box) has been largely conserved among protein encoding genes (transcribed by ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/301167a0
更新日期：1983-01-13 00:00:00
abstract：:During embryonic development, orderly patterns of gene expression eventually assign each cell in the embryo its particular fate. For the anteroposterior axis of the Drosophila embryo, the first step in this process depends on a spatial gradient of the maternal morphogen Bicoid (Bcd). Positional information of this gra...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/415798a
更新日期：2002-02-14 00:00:00
abstract：:The metabolic basis of the autosomal recessive disease cystic fibrosis (CF) remains unidentified. Elevated levels of a serum protein in CF homozygotes and obligate heterozygotes have been described. As heterozygotes are clinically unaffected, any consistently observed abnormality in these individuals is a likely point...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/326614a0
更新日期：1987-04-09 00:00:00
abstract：:Ultraluminous X-ray sources are extragalactic objects located outside the nucleus of the host galaxy with bolometric luminosities exceeding 10(39) erg s(-1). These extreme luminosities-if the emission is isotropic and below the theoretical (Eddington) limit, where the radiation pressure is balanced by the gravitationa...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08083
更新日期：2009-07-02 00:00:00
abstract：:Hox genes are essential for growth and patterning of the tetrapod limb skeleton. Mice mutant for the Hoxd-13 gene have an important delay in morphogenesis owing to reduced proliferation. Based on the appearance of atavisms in such mice, we suggested that modifications of Hox gene regulation may have been a source of m...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/375678a0
更新日期：1995-06-22 00:00:00
abstract：:Recurring outbursts associated with matter flowing onto compact stellar remnants (such as black holes, neutron stars and white dwarfs) in close binary systems provide a way of constraining the poorly understood accretion process. The light curves of these outbursts are shaped by the efficiency of angular-momentum (and...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature25159
更新日期：2018-02-01 00:00:00
abstract：:The population vector hypothesis was introduced almost twenty years ago to illustrate that a population vector constructed from neural activity in primary motor cortex (MI) of non-human primates could predict the direction of hand movement during reaching. Alternative explanations for this population signal have been ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35093102
更新日期：2001-09-13 00:00:00
abstract：:There are major impediments to finding improved DEET alternatives because the receptors causing olfactory repellency are unknown, and new chemicals require exorbitant costs to determine safety for human use. Here we identify DEET-sensitive neurons in a pit-like structure in the Drosophila melanogaster antenna called t...
journal_title：Nature
pub_type： 杂志文章,收录出版
doi：10.1038/nature12594
更新日期：2013-10-24 00:00:00
abstract：:DNA-binding repressor proteins mediate regulation of yeast genes by cell type (Mcm1/alpha 2 and a1/alpha 2), glucose (Mig1) and oxygen (Rox1) (refs 1-4 respectively). An unusual feature of all these regulatory pathways is that transcriptional repression requires two physically associated proteins that do not bind DNA ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/369758a0
更新日期：1994-06-30 00:00:00
abstract：:Cyclin-dependent kinase 5 (Cdk5) and its neuron-specific regulator p35 are essential for neuronal migration and for the laminar configuration of the cerebral cortex. In addition, p35/Cdk5 kinase concentrates at the leading edges of axonal growth cones and regulates neurite outgrowth in cortical neurons in culture. The...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/26034
更新日期：1998-09-10 00:00:00
abstract：:Non-volcanic tremor is a weak, extended duration seismic signal observed episodically on some major faults, often in conjunction with slow slip events. Such tremor may hold the key to understanding fundamental processes at the deep roots of faults, and could signal times of accelerated slip and hence increased seismic...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05666
更新日期：2007-03-15 00:00:00
abstract：:The taxonomy of a parasite can be an important guide to its pathogenic characteristics. A wide range of anatomical, biochemical and behavioural tests is now being developed to define different strains and subspecies of the main tropical parasites. ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/273600a0
更新日期：1978-06-22 00:00:00
abstract：:A family of G-protein-coupled chemoattractant receptors is known to mediate the transport and activation of neutrophils and macrophages This family includes receptors for chemokines, such as interleukin-8, bacterial formylated peptides, platelet-activating factor, leukotriene B4, and the complement anaphylatoxins. The...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/383086a0
更新日期：1996-09-05 00:00:00
abstract：:Cancer genetics has for many years focused on mutational events that have their primary effect within the cancer cell. Recently that focus has widened, with evidence of the importance of epigenetic events and of cellular interactions in cancer development. The role of common genetic variation in determining the range ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/35077207
更新日期：2001-05-17 00:00:00
abstract：:Nonrandom chromosome rearrangements, observed in a variety of human and animal tumours, are associated in some cases with enhanced expression or deregulation of cellular oncogenes. Recently, it was shown that normal, diploid rodent cells are neoplastically transformed following transfection with two cooperating oncoge...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/316636a0
更新日期：1985-08-15 00:00:00
abstract：:Major histocompatibility complex (MHC) class II molecules are highly polymorphic cell-surface glycoproteins that present antigenic peptides to CD4+ T lymphocytes. The normal assembly of class II molecules with cognate peptides for antigen presentation requires an accessory function provided by a gene mapping to the cl...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/368554a0
更新日期：1994-04-07 00:00:00
abstract：:Williams syndrome is a genetic neurodevelopmental disorder characterized by an uncommon hypersociability and a mosaic of retained and compromised linguistic and cognitive abilities. Nearly all clinically diagnosed individuals with Williams syndrome lack precisely the same set of genes, with breakpoints in chromosome b...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature19067
更新日期：2016-08-18 00:00:00
abstract：:The UK Farm-Scale Evaluations (FSE) compared the effects on biodiversity of management of genetically modified herbicide-tolerant (GMHT) spring-sown crops with conventional crop management. The FSE reported larger weed abundance under GMHT management for fodder maize, one of three crops studied. Increased seed product...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02374
更新日期：2004-03-18 00:00:00
abstract：:The Ras proto-oncogene is a central component of mitogenic signal-transduction pathways, and is essential for cells both to leave a quiescent state (G0) and to pass through the G1/S transition of the cell cycle. The mechanism by which Ras signalling regulates cell-cycle progression is unclear, however. Here we report ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/386177a0
更新日期：1997-03-13 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 历史文章,新闻
doi：10.1038/d41586-018-07518-3
更新日期：2018-11-01 00:00:00
abstract：:One of the most exciting events in recent years in molecular biology was the discovery of the left-handed Z form of the DNA double helix. Originally found in linear self-complementary d(GC)x . d(GC)x polymers and oligomers in non-physiological conditions (a rather high salt concentration), it was recently shown to be ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/307481a0
更新日期：1984-02-02 00:00:00
abstract：:The TGF-beta/activin/BMP cytokine family signals through serine/threonine kinase receptors, but how the receptors transduce the signal is unknown. The Mad (Mothers against decapentaplegic) gene from Drosophila and the related Sma genes from Caenorhabditis elegans have been genetically implicated in signalling by membe...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/381620a0
更新日期：1996-06-13 00:00:00
abstract：:Genome instability is central to ageing, cancer and other diseases. It is not only proteins involved in DNA replication or the DNA damage response (DDR) that are important for maintaining genome integrity: from yeast to higher eukaryotes, mutations in genes involved in pre-mRNA splicing and in the biogenesis and expor...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13374
更新日期：2014-07-17 00:00:00
abstract：:Treatments that target immune checkpoints, such as the one mediated by programmed cell death protein 1 (PD-1) and its ligand PD-L1, have been approved for treating human cancers with durable clinical benefit. However, many patients with cancer fail to respond to compounds that target the PD-1 and PD-L1 interaction, an...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature25015
更新日期：2018-01-04 00:00:00
