Abstract：
Objectives:Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and secretion. It also has a role in depolymerization of alginates. Using AlgLs in cystic fibrosis patients along with antibiotics enhances bacterial killing and host healing. In this study, we investigated the different biochemical properties of a newly isolated AlgL from P. aeruginosa S21 to complete the databank of AlgLs. Materials and Methods:The enzyme was extracted from the periplasmic space of the bacteria by the heat shock method. Using the TBA method, the enzyme activity and biochemical properties were assessed. The mutability of P. aeruginosa S21 AlgL to increase its thermal stability was investigated. The most favorable mutations were studied computationally. The molecular dynamics simulation (MDS) package GROMACS was used for determining the effect of S34R mutation on enzyme's thermal stability. Results:Data showed that this enzyme has the best activity at 37 °C and pH 7.5 and it can degrade mannuronate blocks, guluronate blocks, and sodium alginate. After 7 hr at 80 °C, 45% of the enzyme activity was retained. This enzyme needed 15 min to completely degrade accessible sodium alginate. Tris buffer, pH 8.5 and Britton-Robinson buffer, pH 7.0 were the preferable buffers for the enzyme activity. MDS of native and mutated enzymes showed desirable results. Conclusion:P. aeruginosa S21 AlgL can be used in medical and industrial applications to degrade alginates.
journal_name
Iran J Basic Med Scijournal_title
Iranian journal of basic medical sciencesauthors
Piroozmand F,Ghadam P,Zarrabi M,Abdi-Ali Adoi
10.22038/ijbms.2020.37277.8874subject
Has Abstractpub_date
2020-04-01 00:00:00pages
454-460issue
4eissn
2008-3866issn
2008-3874journal_volume
23pub_type
杂志文章abstract：Objectives:Weight gain and metabolic disturbances such as dyslipidemia, are frequent side effects of second-generation antipsychotics, including olanzapine. This study examined the metabolic effects of chronic olanzapine exposure. In addition, we investigated the hepatic fatty acid effects of olanzapine in female C57BL...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.22759.5780
更新日期：2018-05-01 00:00:00
abstract：OBJECTIVE(S):Gastric cancer is the first and second leading cause of cancer related death in Iranian men and women, respectively. Gastric cancer management is based on the surgery, radiotherapy and chemotherapy. In the present study, for the first time, the beneficial effect of saffron (Crocus sativus L.) aqueous extra...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVES:Fumonisins are a group of toxic and carcinogenic mycotoxins, which contaminate the grains and their products. The aim of this study was to examine the apoptotic and proliferative activity of mouse gastric mucosa following administration of fumonisin B1 (FB1). MATERIALS AND METHODS:Twenty-nine female mice di...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：Objectives:Interleukin-36 receptor antagonist (IL-36Ra) is a new member of the IL-1 family that exhibits anti-inflammatory activity in a variety of inflammatory and immune diseases. Our purpose was to determine the effect of IL-36Ra on liver injury in a mouse hepatitis model induced by concanavalin A (ConA). Materials...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.35614.8492
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVES:Tadalafil can restore endothelial function and treat atherosclerosis. However, the effect of tadalafil on transplant arteriosclerosis remains unclear. In this study, we explore the effects of tadalafil on allograft vasculopathy. MATERIALS AND METHODS:Male Brow-Norway rats supplied aorta grafts for Male Lewi...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：Objectives:Ferula ovina is an Iranian medicinal plant. Tschimgine and stylosin are two of its major monoterpene derivatives. In this study, we proceeded to investigate some fungal endophytes from F. ovina that can produce plant secondary metabolites. Materials and Methods:The isolated endophytic fungi were fermented i...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.46334.10703
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:Asthma results from the interaction between genetic and environmental factors. ADAM33 gene on chromosome 20p13 is associated with asthma and airway hyperresponsiveness. MATERIALS AND METHODS:This is a case-control study, where four SNPs S1 (rs3918396), T1 (rs2280091), T2 (rs2280090), V4 (rs2787094) of ADAM3...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：Objectives:The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats. Materials and Methods:Fifty-six female albino Wistar rats were divided into 7 equal groups. Group 1; Sham Op...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2018.30149.7268
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVES:Hepatitis C is a major cause of liver failure worldwide. Current therapies applied for this disease are not fully effective and produce side effects in most cases. Non-structural protein 3 helicase (NS3) of HCV is one of the key enzymes in viral replication and infection. Therefore, this region is a promisin...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE(S):Non-Hodgkin's lymphoma (NHL) is a lymphoproliferative malignancy in which cells undergo microscopic changes with unknown etiology, and risk factors such as age, sex, genetic and environmental factors are involved. The relationship between the presence of infectious agents and the development of lymphoproli...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVES:The primary objective of this study was to determine whether local and imported cigarette brands used in , have elevated levels of metals or not. The produced data of cigarette brands are compared both with each other and with the existing brands in different countries. MATERIALS AND METHODS:In present stud...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：Objectives:The present paper aims to review the studies describing the interactions between HopQ and CEACAMs along with possible mechanisms responsible for pathogenicity of Helicobacter pylori. Materials and Methods:The literature was searched on "PubMed" using different key words including Helicobacter pylori, CEACAM...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：10.22038/ijbms.2019.34237.81
更新日期：2019-07-01 00:00:00
abstract：Objectives:This study aimed to investigate the glucose and lipid metabolism improving effect of the total flavonoids from Selaginella tamariscina (Beauv.) Spring (TFST) on db/db mice, and to study its mechanism of action. Materials and Methods:The db/db mice were divided into 5 groups: the normal group (NC), the diabe...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.40532.9594
更新日期：2020-10-01 00:00:00
abstract：Objectives:Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins f...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.24280.6064
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVES:The Muller cell is the principal glial cell of the vertebrate retina. The expression of Glial fibrillary acidic protein (GFAP) in the Muller cells was used as a cellular marker for retinal damage. This study was done to evaluate the effect of gestational diabetes on retinal Muller cells in rat's offspring. ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2017.8251
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVES:Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton(,)s jelly stem cell) with bone marrow cell mobilized by gr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：Objectives:Adipose-derived stem cells (ADSCs), with suitable and easy access, are multipotential cells that have the ability for differentiation into other mesodermal and transdifferentiate into neural phenotype cells. In this study, Lithium chloride (LiCl) was used for in vitro transdifferentiation of rat ADSCs into n...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.41582.9820
更新日期：2020-04-01 00:00:00
abstract：Objectives:Periodontal diseases are among prevalent oral health problems which may ultimately lead to severe complications in oral cavity. Herbal products can be designed as single or multicomponent preparations for better oral health. This study aims to review current clinical trials on the effectiveness of herbal pro...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：10.22038/IJBMS.2018.31997.7690
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVES:Many cancer cells show significant resistance to drugs that kill drug sensitive cancer cells and non-tumor cells and such resistance might be a consequence of the difference in metabolism. Therefore, studying the metabolism of drug resistant cancer cells and comparison with drug sensitive and normal cell lin...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVES:Primary or adult type hypolactasia, the most common enzyme deficiency in the world, is due to reduced lactase activity in the intestinal cell after weaning. Lactase non-persistence is inherited as an autosomal recessive trait. A DNA variant, single nucleotide polymorphism C/T-13910 which is located on 13910 ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVES:Intracerebral injection of bone marrow stromal cells (BMSCs) is being investigated as a therapeutic tool to prevent Alzheimer's disease (AD). Our aim was to investigate the effects of BMSCs by intrathecal injection in AD rat model. MATERIALS AND METHODS:BMSCs were obtained from the bone marrow of Wistar rat...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：OBJECTIVES:Both caloric restriction (CR) and resveratrol (RSV) have been shown to improve learning and memory, but their potential effects in juvenile animals were unknown. Here, we evaluated the effects of RSV and CR on learning and memory function in juvenile mice and investigated potential molecular mechanisms. MET...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：Objectives:In this study, we investigated the protective effects of piperine on lead acetate-induced renal damage in rat kidney tissue. Materials and Methods:Forty male rats were divided into 5 groups: negative control (rats were given aquadest daily), positive control (rats were given lead acetate 30 mg/kg BW orally ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2017.9487
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVES:Prostaglandins have been shown to mediate the gastro-protective effect of sodium hydrosulfide (NaHS) but effect of NaHS on mRNA expression of prostaglandin E2 receptors (EP1, 3-4; EPs) has not been investigated. Therefore, this study designed to evaluate the effect of NaHS on mRNA expression of EPs receptors...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2017.8237
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVE(S):Atherosclerosis is a chronic immune-inflammatory disease that generally leads to ischemic heart disease. Ghrelin has several modulatory effects on cardiovascular system. In this study, we investigated the effect of ghrelin on aortic intima-media thickness, size and the number of adipocyte cells in obese an...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE(S):Garlic (Allium sativum L. family Liliaceae) is well known in Iran and its leaves, flowers, and cloves have been used in traditional medicine for a long time. Research in recent decades has shown widespread pharmacological effects of A. sativum and its organosulfur compounds especially Allicin. Studies carr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVES:In food industry, the inhibition of tyrosinase is very important, because this enzyme catalyzes the oxidation of phenolic compounds found in fruits and vegetables into quinones, which contribute in undesirable color and taste of fruits and vegetables. Teucrium polium L. var. gnaphalodes (Lamiaceae), a wild-g...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：Objectives:A growing body of evidence indicates that rhomboid domain containing 1 (RHBDD1) plays an important role in a variety of physiological and pathological processes, including tumorigenesis. We aimed to determine the function of RHBDD1 in breast cancer cells. Materials and Methods:In this study, we used the Onc...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.29788.7191
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE(S):The effect of the extract of Crocus sativus and its constituent, safranal on inflammatory markers in sensitized guinea pigs was examined. MATERIALS AND METHODS:Ovalbumin (OA) sensitized guinea pigs were given drinking water alone (group S), or drinking water containing three concentrations of safranal, th...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVES:Valproic acid (VPA) has proven to be as one of the most promising useful drug with anticancer properties. In this study, we investigate the VPA effects on E-cadherin expression in HeLa, TC1, MKN45, and HCT116 cell lines. This study assesses the effects of VPA on human papillomavirus E7 expression in HPV posi...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-06-01 00:00:00
