Abstract：
OBJECTIVES:Hepatitis C is a major cause of liver failure worldwide. Current therapies applied for this disease are not fully effective and produce side effects in most cases. Non-structural protein 3 helicase (NS3) of HCV is one of the key enzymes in viral replication and infection. Therefore, this region is a promising target to design new drugs and therapies against HCV infection. The aim of this study was cloning and expression of HCV NS3 helicase fragment in Escherichia coli BL21 (DE3) using pET102/D-TOPO expression vector and studying immunoreactivity of the expressed antigen in Iranian infected with hepatitis C. MATERIALS AND METHODS:The viral RNA was extracted from the serum of HCV infected patient. The NS3 helicase region was amplified by RT-PCR. The PCR product was directionally cloned into the expression vector pET102/D-TOPO and transformed into the BL21 strain of E. coli (DE3). The transformed bacteria were then induced by adding 1mM isopropyl-β-D-thiogalactopyranoside (IPTG) into the culture medium to enhance the protein expression. SDS-PAGE and western blotting were carried out to identify the protein under investigation, and finally purified recombinant fusion protein was used as the antigen for ELISA method. RESULTS:The insertion of the DNA fragment of the NS3 region into the expression vector was further confirmed by PCR and sequencing. SDS-PAGE analysis showed the successful expression of the recombinant protein of interest. Furthermore, immunoreactivity of fusion NS3 helicase was confirmed by ELISA and western blotting. CONCLUSION:It seems that this recombinant protein could be a useful source of antigen for future studies on HCV diagnosis and therapy.
journal_name
Iran J Basic Med Scijournal_title
Iranian journal of basic medical sciencesauthors
Sadri M,Farajollahi MM,Sharifi Zsubject
Has Abstractpub_date
2015-02-01 00:00:00pages
159-63issue
2eissn
2008-3866issn
2008-3874journal_volume
18pub_type
杂志文章abstract：OBJECTIVE(S):In the current study, the effects of selected folk medicinal herbs were evaluated in D-galactose-induced aging in male mice. MATERIALS AND METHODS:Male BALB/c mice were randomly divided into 12 groups composing sham, control, and treated groups. Aging was induced by administration of D-galactose (500 mg/k...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE(S):PDE3 has a functional role in insulin secretion and action. We investigated the metabolic effects of new synthetic PDE3 inhibitors (mc1, mc2, mc5 and mc6), on mice and hyperglycemic rat. MATERIALS AND METHODS:The test compound or solvent was injected subcutaneously to mice, for 7 days. On day 8, blood and...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：:Sulfur mustard (SM, bis- (2-chloroethyl) sulphide) is a chemical warfare agent that causes DNA alkylation, protein modification and membrane damage. SM can trigger several molecular pathways involved in inflammation and oxidative stress, which cause cell necrosis and apoptosis, and loss of cells integrity and function...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVES:Hyperthermia is one of the most common environmental stressors that affect multi-biological systems in the body including the central nervous system as well as the hematopoietic organs. The objective of the present study was to investigate the protective role of ethanolic extract of propolis (EEP) on some se...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：Objectives:Morbidity and mortality due to diabetes mellitus (DM) result in exorbitant psycho-economical costs, so there is a strong need to create new strategies and drugs for controlling DM. The aim of the current study was to investigate the anti-diabetic effect of the aqueous extract of Pterocarpus santalinus on str...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.34998.8325
更新日期：2019-08-01 00:00:00
abstract：Objectives:Association of adolescent emotional stress (ES) with the incidence of cardiovascular disease (CVD) at older age was investigated. Materials and Methods:21 female rats were divided into three groups of 7 each; ES, foot-shock, and control. Chronic ES was induced by exposing the rats to witness foot-shock of t...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.32888.7857
更新日期：2019-10-01 00:00:00
abstract：OBJECTIVES:MicroRNAs (miRNAs) are a class of short RNAs that control the biological processes including cell proliferation, apoptosis and development. Aberrant expression of miRNAs was determined in the different stages of tumor development and metastasis. To study the effect of silibinin on miRNAs expression, we evalu...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-12-01 00:00:00
abstract：Objectives:Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. Materials and Methods:Saline, acetylcholine (Ach; doses: 90 and 150 nmol), hexamethonium (Hexa; ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.25616.6319
更新日期：2018-04-01 00:00:00
abstract：Objectives:We investigated the role of nitric oxide (NO) in the protective effects of remote ischemic per-conditioning (rIPerC) on renal ischemia/reperfusion (I/R) injury in male rats. Materials and Methods:I/R treatment consisted of 45 min bilateral renal artery ischemia and 24 hr reperfusion interval. rIPerC was per...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.25810.6354
更新日期：2018-06-01 00:00:00
abstract：OBJECTIVE(S):Carotenoids which are naturally synthesized by fungi such as yeasts can act as an antioxidant which is closely related to their ability to decrease the risk of a variety of degenerative diseases. In recent years, the increase of demand for carotenoids obtained from natural sources has promoted major effort...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：Objectives:A growing body of evidence indicates that rhomboid domain containing 1 (RHBDD1) plays an important role in a variety of physiological and pathological processes, including tumorigenesis. We aimed to determine the function of RHBDD1 in breast cancer cells. Materials and Methods:In this study, we used the Onc...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.29788.7191
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVES:Lupus nephritis (LN) is frequently associated with a poor long-term prognosis. Renal biopsy is the diagnostic method of choice in this condition. Urine biomarkers have been mentioned in the diagnosis of LN. The study(,)s purpose was to evaluate the performance of urinary monocyte chemoattractant protein 1(UM...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVES:In previous studies, researchers observed that doxepin could improve cognitive processes and has protective effects on the central nervous system. Thus, this study was designed to analyze the effects of doxepin on β-amyloid (Aβ)-induced memory impairment and neuronal toxicity in rat and to explore the underl...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2017.9274
更新日期：2017-09-01 00:00:00
abstract：Objectives:Charcot-Marie Tooth disease (CMT) is one of the main inherited causes of motor and sensory neuropathies with variable expressivity and age-of onset. Although more than 70 genes have been identified for CMT, more studies are needed to discover other genes involved in CMT. Introduction of whole exome sequencin...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.30754.7414
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE(S):Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast an...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：Objectives:Periodontal diseases are among prevalent oral health problems which may ultimately lead to severe complications in oral cavity. Herbal products can be designed as single or multicomponent preparations for better oral health. This study aims to review current clinical trials on the effectiveness of herbal pro...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：10.22038/IJBMS.2018.31997.7690
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVE(S):Atherosclerosis is a chronic immune-inflammatory disease that generally leads to ischemic heart disease. Ghrelin has several modulatory effects on cardiovascular system. In this study, we investigated the effect of ghrelin on aortic intima-media thickness, size and the number of adipocyte cells in obese an...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVES:Neuroprotective effect of creatine (Cr) against β-amyloid (Aβ) is reported in an in vitro study. This study investigated the effect of Cr supplementation on β-amyloid toxicity in vivo. MATERIALS AND METHODS:Thirty two, male Wistar rats were divided into 4 groups. During ten weeks of study, control group wen...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-11-01 00:00:00
abstract：OBJECTIVES:The purpose of the current study was to assess the feasibility of microspheres from biocompatible polymer for oral bioavailability (BA) enhancement of potent sulfonamide- type loop diuretic- Furosemide - which used in the treatment of congestive heart failure, caused edema, cirrhosis, renal disease and as an...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：:Alzheimer's disease (AD) is a neurodegenerative disease and the leading cause of dementia worldwide. Epidemiological studies support the important role of diet in prevention and improvement of AD. In Traditional Persian Medicine (TPM), there is multiple dietary guidelines to prevent and alleviate dementia and memory i...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：10.22038/ijbms.2019.36505.8694
更新日期：2019-10-01 00:00:00
abstract：Objectives:Neurodegeneration is an outcome of Methamphetamine (METH) abuse. Studies have emphasized on the neuroprotective properties of lithium. The current study is designed towards evaluating the role of Akt-1/GSK3 and CREB-BDNF signaling pathways in mediating lithium neuroprotection against METH-induced neurodegene...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.30855.7442
更新日期：2019-08-01 00:00:00
abstract：OBJECTIVES:In food industry, the inhibition of tyrosinase is very important, because this enzyme catalyzes the oxidation of phenolic compounds found in fruits and vegetables into quinones, which contribute in undesirable color and taste of fruits and vegetables. Teucrium polium L. var. gnaphalodes (Lamiaceae), a wild-g...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE(S):Because of the high total phenolic contents of Feijoa sellowiana, its nephroprotective effect was determined in 3,4-methylene dioxymethamphetamine (MDMA) treated mice. MATERIALS AND METHODS:Animals received 10, 20 and 40 mg/kg doses of aqueous or methanol extract of F. sellowiana leaves, intra-peritoneall...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE(S):Garlic (Allium sativum L. family Liliaceae) is well known in Iran and its leaves, flowers, and cloves have been used in traditional medicine for a long time. Research in recent decades has shown widespread pharmacological effects of A. sativum and its organosulfur compounds especially Allicin. Studies carr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVES:Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton(,)s jelly stem cell) with bone marrow cell mobilized by gr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：Objectives:Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim of the present study was to provide a 3D organ system for determinati...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.30056.7242
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVES:Parkinson's disease (PD) is a progressive neurological disorder associated with motor disabilities and cognitive dysfunction as well. Evidence indicates that PD occurs less frequently in women than men, confirming a role for steroid hormones in protection of dopaminergic nigrostriatal neurons. It is reported...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2016.7911
更新日期：2016-12-01 00:00:00
abstract：Objectives:Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogen, causes 1.5 million deaths globally. Bacilli Calmette-Guérin (BCG) is commonly administered to protect people against M. tuberculosis infection; however, there are some obstacles with this first-generation vaccine. DNA vaccines, the thir...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.41806.9881
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVES:Lead (Pb) is a toxic metal inducing many destructive effects leading to a broad range of physiological, biochemical, and neurological dysfunctions in humans and laboratory animals. MATERIALS AND METHODS:Here, we investigated the effect of chronic exposure to Pb (50 mg/l) on oxidative stress, hepatotoxicity,...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：Objectives:Chemotherapies used to treat colon cancer might often fail due to the emergence of chemoresistance and side effects. Escherichia coli Nissle 1917 (EcN) is a beneficial probiotic, whose molecular mechanisms in the prevention of colon cancer are yet to be fully understood. The present study assessed the anti-c...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.43016.10115
更新日期：2020-07-01 00:00:00
