Abstract：
Objectives:Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogen, causes 1.5 million deaths globally. Bacilli Calmette-Guérin (BCG) is commonly administered to protect people against M. tuberculosis infection; however, there are some obstacles with this first-generation vaccine. DNA vaccines, the third generation vaccines, can induce cellular immune responses for tuberculosis (TB) protection. In this study, optimized DNA vaccine (pcDNA3.1-Mtb72F) entrapped in poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) was used to achieve higher immunogenicity. Materials and Methods:Plasmid Mtb72F was formulated in PLGA NPs using double emulsion method in the presence of TB10.4 and/or CpG as an adjuvant. Female BALB/c mice were immunized either with NP-encapsulated Mtb72F or naked Mtb72F with or without each adjuvant, using the BCG-prime DNA boost regimen. Results:These NPs were approximately 250 nm in diameter and the nucleic acid and protein encapsulation efficiency were 80% and 25%, respectively. The NPs smaller than 200 nm are able to promote cellular rather than humoral responses. The immunization with the formulation consisting of Mtb72F DNA vaccine and TB10.4 entrapped in PLGA NPs showed significant immunogenicity and induced predominantly interferon-ɣ (IFN-ɣ) production and higher INF-ɣ/interleukin-4 (IL-4) ratio in the cultured spleen cells supernatant. Conclusion:PLGA NPs loaded with Mtb72F DNA-based vaccine with TB10.4 could be considered as a promising candidate for vaccination against TB. These results represent an excellent initial step toward development of novel vaccine for TB protection.
journal_name
Iran J Basic Med Scijournal_title
Iranian journal of basic medical sciencesauthors
Dalirfardouei R,Tafaghodi M,Meshkat Z,Najafi A,Gholoobi A,Nabavinia MS,Sajedifar S,Meshkat M,Badiee A,Ramezani M,Varasteh AR,Naderinasab Mdoi
10.22038/ijbms.2020.41806.9881subject
Has Abstractpub_date
2020-06-01 00:00:00pages
826-832issue
6eissn
2008-3866issn
2008-3874journal_volume
23pub_type
杂志文章abstract：Objectives:Adipose-derived stem cells (ADSCs), with suitable and easy access, are multipotential cells that have the ability for differentiation into other mesodermal and transdifferentiate into neural phenotype cells. In this study, Lithium chloride (LiCl) was used for in vitro transdifferentiation of rat ADSCs into n...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.41582.9820
更新日期：2020-04-01 00:00:00
abstract：OBJECTIVES:Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton(,)s jelly stem cell) with bone marrow cell mobilized by gr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVES:Fabaceae is the third largest family of flowering plants. Lack of essential oils in the plants of this family can be considered as an advantage and can favor them in search for safe and antiepileptic medicines. The effects of Fabacea family plants including Ebenus stellata (E. stellata), Sophora alopecuroide...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：:Rosa damascena mill L., known as Gole Mohammadi in is one of the most important species of Rosaceae family flowers. R. damascena is an ornamental plant and beside perfuming effect, several pharmacological properties including anti-HIV, antibacterial, antioxidant, antitussive, hypnotic, antidiabetic, and relaxant effec...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVES:Many cancer cells show significant resistance to drugs that kill drug sensitive cancer cells and non-tumor cells and such resistance might be a consequence of the difference in metabolism. Therefore, studying the metabolism of drug resistant cancer cells and comparison with drug sensitive and normal cell lin...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVES:Similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. Reprogramming-related transcription factors, also known as Yamanaka factors (OCT4, SOX2, KLF4, and c-MYC), are also well-known oncogenes promoting cancer initiation, pr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：Objectives:Prompt detection of extended-spectrum β-lactamases (ESBL) and carbapenemase-producing enterobacteriaceae is crucial for infection prevention and control strategies. The present study aimed to characterize the ESBL and carbapenemase genes among Enterobacter isolates from an Iranian inpatient population. Mate...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.41225.9752
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVES:Cyclooxygenases (COXs) are known to play some roles in physiological mechanisms related to learning and memory. Since sodium salicylate is an inhibitor of COX, we have evaluated the effect of peripheral and intra-hippocampal administration of sodium salicylate on spatial learning and memory in male rats. MA...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVES:Morphine is widely used to treat chronic pain. However, its utility is hindered by the development of tolerance to its analgesic effects. Despite the renowned beneficial effects of physical exercise on cognitive functions and signs of morphine withdrawal in morphine-dependent rats, little is known about the ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE(S):The effect of the extract of Crocus sativus and its constituent, safranal on inflammatory markers in sensitized guinea pigs was examined. MATERIALS AND METHODS:Ovalbumin (OA) sensitized guinea pigs were given drinking water alone (group S), or drinking water containing three concentrations of safranal, th...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVES:Recently, we showed that some new synthetic compounds structurally related to cilostamide (4-(1,2-dihydro-2-oxoquinolin-6-hydroxy)- N-cyclohexyl-N-methylbutanamide), a selective phosphodiesterase 3 (PDE3) inhibitor, produce inotropic effect comparable to that of IBMX (3-isobutyl-1-methylxanthine), a non-sele...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2017.8831
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVE(S):Pre-eclampsia is characterized by hypertension, dyslipidemia, and increased systemic inflammatory response and has been associated with an increased maternal risk of cardiovascular disease later in life. Endothelial dysfunction is thought to be a central pathogenic feature in pre-eclampsia on the basis of ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVES:Eukaryotic translation initiation factor 4E (eIF4E) is overexpressed in cervical cancer (CC). However, the molecular mechanisms are unclear. This study aimed to investigate the molecular mechanism of eIF4E gene overexpression in CC. MATERIALS AND METHODS:The human papillomavirus (HPV) type 18 E7 and eIF4E m...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE(S):Non-Hodgkin's lymphoma (NHL) is a lymphoproliferative malignancy in which cells undergo microscopic changes with unknown etiology, and risk factors such as age, sex, genetic and environmental factors are involved. The relationship between the presence of infectious agents and the development of lymphoproli...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：Objectives:Prenatal stresses increase incidence of neurodevelopmental disorders and influence cognitive abilities. Glucocorticoids are released in stress condition as endpoint activation of hypothalamus-pituitary-adrenal (HPA) axis. Evidence indicates a cross-talk between gut microbiota and brain function. This study a...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.33956.8078
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVES:Hepatitis C is a major cause of liver failure worldwide. Current therapies applied for this disease are not fully effective and produce side effects in most cases. Non-structural protein 3 helicase (NS3) of HCV is one of the key enzymes in viral replication and infection. Therefore, this region is a promisin...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE(S):Because of the high total phenolic contents of Feijoa sellowiana, its nephroprotective effect was determined in 3,4-methylene dioxymethamphetamine (MDMA) treated mice. MATERIALS AND METHODS:Animals received 10, 20 and 40 mg/kg doses of aqueous or methanol extract of F. sellowiana leaves, intra-peritoneall...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVES:MicroRNAs (miRNAs) are a class of short RNAs that control the biological processes including cell proliferation, apoptosis and development. Aberrant expression of miRNAs was determined in the different stages of tumor development and metastasis. To study the effect of silibinin on miRNAs expression, we evalu...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-12-01 00:00:00
abstract：Objectives:Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim of the present study was to provide a 3D organ system for determinati...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.30056.7242
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVES:Tadalafil can restore endothelial function and treat atherosclerosis. However, the effect of tadalafil on transplant arteriosclerosis remains unclear. In this study, we explore the effects of tadalafil on allograft vasculopathy. MATERIALS AND METHODS:Male Brow-Norway rats supplied aorta grafts for Male Lewi...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVES:Lipocalin 2 (Lcn2) is a 25-kDa glycoprotein that has initially been extracted from neutrophil granules. Expression of Lcn2 is induced under various pathophysiological conditions. It is also known as an early marker of kidney and heart injury. High-level expression of recombinant Lcn2 neutrophil gelatinase-as...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：Objectives:Neurodegeneration is an outcome of Methamphetamine (METH) abuse. Studies have emphasized on the neuroprotective properties of lithium. The current study is designed towards evaluating the role of Akt-1/GSK3 and CREB-BDNF signaling pathways in mediating lithium neuroprotection against METH-induced neurodegene...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.30855.7442
更新日期：2019-08-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the methylation status and mRNA expression levels of P15, death-associated protein kinase (DAPK), and suppressor of cytokine signaling-1 (SOCS1) genes in multiple myeloma (MM). MATERIALS AND METHODS:The bone marrow samples of 54 MM patients were collected and the met...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVES:Neuroprotective effect of creatine (Cr) against β-amyloid (Aβ) is reported in an in vitro study. This study investigated the effect of Cr supplementation on β-amyloid toxicity in vivo. MATERIALS AND METHODS:Thirty two, male Wistar rats were divided into 4 groups. During ten weeks of study, control group wen...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-11-01 00:00:00
abstract：Objectives:Eupafolin, a major active component of Eupatorium perfoliatum L., has anti-inflammatory and anti-oxidant properties. Lipopolysaccharide (LPS) is responsible for myocardial depression. A line of evidences revealed that LPS induces autophagy in cardiomyocytes injury. This study aims to evaluate the effects of ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.37748.8977
更新日期：2019-11-01 00:00:00
abstract：Objectives:We investigated the role of nitric oxide (NO) in the protective effects of remote ischemic per-conditioning (rIPerC) on renal ischemia/reperfusion (I/R) injury in male rats. Materials and Methods:I/R treatment consisted of 45 min bilateral renal artery ischemia and 24 hr reperfusion interval. rIPerC was per...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.25810.6354
更新日期：2018-06-01 00:00:00
abstract：Objectives:Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and secretion. It also has a role in depolymerization of alginates. Using A...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2020.37277.8874
更新日期：2020-04-01 00:00:00
abstract：Objectives:Weight gain and metabolic disturbances such as dyslipidemia, are frequent side effects of second-generation antipsychotics, including olanzapine. This study examined the metabolic effects of chronic olanzapine exposure. In addition, we investigated the hepatic fatty acid effects of olanzapine in female C57BL...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.22759.5780
更新日期：2018-05-01 00:00:00
abstract：OBJECTIVES:Nitric oxide (NO), a product of inducible nitric oxide synthase (iNOS), contributes in germ cell apoptosis. This study was aimed to evaluate the effects of Aloe vera gel (AVG) on male Wistar rat reproductive organ, serum NO level, and expression of iNOS gene in leydig cells. MATERIALS AND METHODS:Adult male...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVES:HTLVI-1 is the first human retrovirus with limited endemic regions in the world. The epidemiological studies have shown that the genetic background and immune response to the virus have a significant role in HTLV-I-associated diseases. Among the genes are involved in HTLV-I infection, the role of human leuko...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
