Silencing of rhomboid domain containing 1 to inhibit the metastasis of human breast cancer cells in vitro.

Abstract:

Objectives:A growing body of evidence indicates that rhomboid domain containing 1 (RHBDD1) plays an important role in a variety of physiological and pathological processes, including tumorigenesis. We aimed to determine the function of RHBDD1 in breast cancer cells. Materials and Methods:In this study, we used the Oncomine™ database to determine the expression patterns of RHBDD1 in normal and breast cancer tissues. We performed lentiviral transfection of RHBDD1-specific small interfering RNA into the breast cancer cell lines ZR-75-30 and MDA-MB-231 in order to investigate the effects of RHBDD1 deficiency on breast cancer metastasis. Results:We found that knockdown of RHBDD1 inhibited breast cancer cell migration and invasion in vitro. Moreover, knockdown of RHBDD1 promoted epithelial-mesenchymal transition (EMT) by suppressing the expression of MPP2, MPP9, fibronectin 1, vimentin, SRY-box 2, zinc finger E-box binding homeobox 1, and snail family transcriptional repressor 1, and promoting the expression of cadherin 1. Additionally, knockdown of RHBDD1 inhibited the protein expression and phosphorylation of Akt. Conclusion:Our data indicate that RHBDD1 overexpression may promote breast cancer metastasis via the regulation of EMT, suggesting that RHBDD1 may be an important regulator of breast cancer metastasis.

journal_name

Iran J Basic Med Sci

authors

Huang C,Ji X,Peng Y,Wu M,Wu W,Luo Y,Cheng G,Zhu Y

doi

10.22038/IJBMS.2018.29788.7191

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

1161-1166

issue

11

eissn

2008-3866

issn

2008-3874

journal_volume

21

pub_type

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