Abstract:
:Ischemic preconditioning (IPre) reduces ischemia/reperfusion (I/R) injury in the heart. The non-coding microRNA miR-125b-1-3p has been demonstrated to play a role in the mechanism of IPre. Hypercholesterolemia is known to attenuate the cardioprotective effect of preconditioning; nevertheless, the exact underlying mechanisms are not clear. Here we investigated, whether hypercholesterolemia influences the induction of miR-125b-1-3p by IPre. Male Wistar rats were fed with a rodent chow supplemented with 2% cholesterol and 0.25% sodium-cholate hydrate for 8 weeks to induce high blood cholesterol levels. The hearts of normo- and hypercholesterolemic animals were then isolated and perfused according to Langendorff, and were subjected to 35 min global ischemia and 120 min reperfusion with or without IPre (3 × 5 min I/R cycles applied before index ischemia). IPre significantly reduced infarct size in the hearts of normocholesterolemic rats; however, IPre was ineffective in the hearts of hypercholesterolemic animals. Similarly, miR-125b-1-3p was upregulated by IPre in hearts of normocholesterolemic rats, while in the hearts of hypercholesterolemic animals IPre failed to increase miR-125b-1-3p significantly. Phosphorylation of cardiac Akt, ERK, and STAT3 was not significantly different in any of the groups at the end of reperfusion. Based on these results we propose here that hypercholesterolemia attenuates the upregulation of miR-125b-1-3p by IPre, which seems to be associated with the loss of cardioprotection.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Szabó MR,Gáspár R,Pipicz M,Zsindely N,Diószegi P,Sárközy M,Bodai L,Csont Tdoi
10.3390/ijms21113744subject
Has Abstractpub_date
2020-05-26 00:00:00issue
11issn
1422-0067pii
ijms21113744journal_volume
21pub_type
杂志文章abstract::Aegista chejuensis and Aegista quelpartensis (Family-Bradybaenidae) are endemic to Korea, and are considered vulnerable due to declines in their population. The limited genetic resources for these species restricts the ability to prioritize conservation efforts. We sequenced the transcriptomes of these species using I...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17030379
更新日期:2016-03-15 00:00:00
abstract::The aim of this study is to elucidate the detailed mechanism of endoplasmic reticulum (ER) stress-induced auditory cell death based on the function of the initiator caspases and molecular complex of necroptosis. Here, we demonstrated that ER stress initiates not only caspase-9-dependent intrinsic apoptosis along with ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20235896
更新日期:2019-11-24 00:00:00
abstract::Absence of effective therapeutic methods for avascular necrosis of femoral head (ANFH) is still perplexing the world's medical community. Bone marrow mesenchymal stem cells (BMSCs) adoptive cell therapy combined with core decompression is a promising modality, which is highly dependent on the cellular activities of BM...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030734
更新日期:2018-03-05 00:00:00
abstract::Interactions between tumor cells and tumor-associated macrophages (TAMs) are critical for glioblastoma progression. The TAMs represent up to 30% of the glioblastoma mass. The role of TAMs in tumor progression and in the mechanisms underlying tumor growth remain unclear. Using an in vitro model resembling the crosstalk...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113990
更新日期:2020-06-02 00:00:00
abstract::In Arabidopsis basal hypocotyls of dark-grown seedlings, xylary cells may form from the pericycle as an alternative to adventitious roots. Several hormones may induce xylogenesis, as Jasmonic acid (JA), as well as indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) auxins, which also affect xylary identity. Stu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184469
更新日期:2019-09-10 00:00:00
abstract::Molecular factors involved in neuroprotection are key in the design of novel multitarget drugs in aging and neurodegeneration. AVCRI104P3 is a huprine derivative that exhibits potent inhibitory effects on human AChE, BuChE, and BACE-1 activities, as well as on AChE-induced and self-induced Aβ aggregation. More recentl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092615
更新日期:2018-09-04 00:00:00
abstract::Dysregulated human monocytes/macrophages can synthesize and secrete matrix metalloproteinases (MMPs), which play important roles in the progression of sepsis. In this study, we investigated the effects and mechanism of a novel histone deacetylase (HDAC8) inhibitor, (E)-N-hydroxy-4-methoxy-2-(biphenyl-4-yl)cinnamide (W...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18071394
更新日期:2017-06-29 00:00:00
abstract::Modern antipsychotic drugs are employed increasingly in the treatment of mood disorders as well as psychoses, stimulating interest in their possible contributions to altering suicidal risk. Clozapine remains the only treatment with an FDA-recognized indication for reducing suicidal risk (in schizophrenia). We carried ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17101700
更新日期:2016-10-11 00:00:00
abstract::Extracellular vesicles (EVs) have been tremendous carriers in both experimental and translational science. These vesicles-formerly regarded as artifacts of in vitro research-have a heterogeneous population of vesicles derived from virtually all eukaryotic cells. EVs consist of a bilayer lipid structure with a diameter...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20235995
更新日期:2019-11-28 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to β-lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in terms of observation of the growth properties and ability to form biofilms in sensitive S. aureu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161024656
更新日期:2015-10-16 00:00:00
abstract::Inflammatory bowel disease (IBD) is a chronic disorder manifested as Crohn's disease (CD) and ulcerative colitis (UC) characterized by intestinal inflammation and involves a dysregulated immune response against commensal microbiota through the activation of CD4 T helper cells. T helper cell differentiation to effector...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21093379
更新日期:2020-05-11 00:00:00
abstract::The growth arrest-specific transcript 5 (GAS5) is a >200-nt lncRNA molecule that regulates several cellular functions, including proliferation, apoptosis, invasion and metastasis, across different types of human cancers. Here, we reviewed the current literature on the expression of GAS5 in leukemia, cervical, breast, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21207633
更新日期:2020-10-15 00:00:00
abstract::Glucose-6-phosphate dehydrogenase (G6PD) deficiency in humans causes severe disease, varying from mostly asymptomatic individuals to patients showing neonatal jaundice, acute hemolysis episodes or chronic nonspherocytic hemolytic anemia. In order to understand the effect of the mutations in G6PD gene function and its ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17050787
更新日期:2016-05-21 00:00:00
abstract::Plants infected with DNA viruses produce massive quantities of virus-derived, 24-nucleotide short interfering RNAs (siRNAs), which can potentially direct viral DNA methylation and transcriptional silencing. However, growing evidence indicates that the circular double-stranded DNA accumulating in the nucleus for Pol II...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140815233
更新日期:2013-07-24 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is among the severe neuro degenerative diseases that lack widely available effective treatments. As the disease progresses, patients lose the control of voluntary muscles. Although the neuronal degeneration is the cause of this disease, the failure mechanism is still unknown. In ord...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051318
更新日期:2018-04-28 00:00:00
abstract::Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205105
更新日期:2019-10-15 00:00:00
abstract::The relationships among 55 wheat accessions (47 accessions collected from Iran and eight accessions provided by the Institute of Plant Biology of the University of Zurich, Switzerland) belonging to eight species carrying A genome (Triticum monococcum L., T. boeoticum Boiss., T. urartu Tumanian ex Gandilyan, T. durum D...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11114309
更新日期:2010-11-02 00:00:00
abstract::Extracellular vesicles (EVs) are lipid bilayer vesicles which are released from cells and play multifaceted roles in cellular communication in health and disease. EVs can be isolated from various body fluids, including serum and plasma, and are usable biomarkers as they can inform health status. Studies on EVs are an ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020875
更新日期:2021-01-16 00:00:00
abstract::SDS-gradient mini-gel electrophoresis and immunoblotting of urine of rats given ochratoxin A (OTA), showed OTA binding to an alpha2u-globulin. Perceived potential internalised delivery of OTA to proximal tubule epithelia by the carrier, specific only to adult male rats and augmenting other uptake mechanisms, suggests ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9050719
更新日期:2008-05-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) typically migrates and metastasizes. Interleukin-6 (IL-6) is a multifunctional cytokine associated with disease status and cancer outcomes. The effect of IL-6 on human OSCC cells, however, is unknown. Here, we showed that IL-6 increased cell migration and Intercellular adhesion mole...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15010545
更新日期:2014-01-06 00:00:00
abstract::Quorum sensing is an intercellular form of communication that bacteria use to coordinate group behaviors such as biofilm formation and the production of antibiotics and virulence factors. The term quorum sensing was originally coined to describe the mechanism underlying the onset of luminescence production in cultures...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140816386
更新日期:2013-08-07 00:00:00
abstract::The epithelial sodium channel (ENaC) has a key role in modulating endothelial cell stiffness and this in turn regulates nitric oxide (NO) synthesis. The physiological relevance of endothelial ENaC in pathological conditions where reduced NO bioavailability plays an essential role remains largely unexplored. Renal isch...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20133132
更新日期:2019-06-27 00:00:00
abstract::Sequence-defined recombinant antibodies (rAbs) have emerged as alternatives to hybridoma-secreted monoclonal antibodies (mAbs) for performing immunoassays. However, the polyploidy nature of hybridomas often leads to the coexistence of aberrant or non-specific functional variable region (VR) gene transcripts, which com...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21186857
更新日期:2020-09-18 00:00:00
abstract::Factor binding IST-1 (FBI-1) plays an important role in oncogenic transformation and tumorigenesis. As FBI-1 is over-expressed in multiple human cancers, the regulation of itself would provide new effective options for cancer intervention. In this work, we aimed to study the role that EPAS-1 plays in regulating FBI-1....
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150915689
更新日期:2014-09-04 00:00:00
abstract::Scaffolds made of biodegradable biomaterials are widely used to guide bone regeneration. Commonly, in vitro assessment of scaffolds' osteogenesis potential has been performed predominantly in monoculture settings. Hence, this study evaluated the potential of an unstimulated, growth factor-free co-culture system compri...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20051068
更新日期:2019-03-01 00:00:00
abstract::Regulation of melanogenesis has been the focus of treatment for hyperpigmentary skin disorders. Although hesperidin is one of the most well-known, naturally occurring flavonoids with antioxidant and anti-inflammatory effect, its anti-melanogenic effect is not known. The present study aims to determine the anti-melanog...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160818384
更新日期:2015-08-07 00:00:00
abstract::The paper presents a detailed study of the biological effects of two amino acid hydroxyurea derivatives that showed selective antiproliferative effects in vitro on the growth of human tumor cell line SW620. Tested compounds induced cell cycle perturbations and apoptosis. Proteins were identified by proteomics analyses...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141223654
更新日期:2013-12-04 00:00:00
abstract::The aim of this study was to assess the effect of naringenin on osteoclastogenesis and titanium particle-induced osteolysis. Osteolysis from wear-induced particles and aseptic loosening are the most frequent late complications of total joint arthroplasty leading to revision of the prosthesis. Osteolysis during aseptic...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151221913
更新日期:2014-11-28 00:00:00
abstract::Carotid atherosclerotic plaque rupture can lead to cerebrovascular accident (CVA). By comparing RNA-Seq data from vascular smooth muscle cells (VSMC) extracted from carotid atheroma surgically excised from a group of asymptomatic and symptomatic subjects, we identified more than 700 genomic variants associated with sy...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249387
更新日期:2020-12-09 00:00:00
abstract::The silk gland synthesizes and secretes a large amount of protein and stores liquid silk protein at an extremely high concentration. Interestingly, silk proteins and serine protease inhibitors are orderly arranged in the silk gland lumen and cocoon shells. Silk fiber formation and the spinning mechanism have not been ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102491
更新日期:2019-05-20 00:00:00