Abstract：
:Interactions between tumor cells and tumor-associated macrophages (TAMs) are critical for glioblastoma progression. The TAMs represent up to 30% of the glioblastoma mass. The role of TAMs in tumor progression and in the mechanisms underlying tumor growth remain unclear. Using an in vitro model resembling the crosstalk between macrophages and glioblastoma cells, we show that glioblastoma-derived exosomes (GBex) reprogram M1 (mediate pro-inflammatory function) and M2 (mediate anti-inflammatory function) macrophages, converting M1 into TAMs and augmenting pro-tumor functions of M2 macrophages. In turn, these GBex-reprogrammed TAMs, produce exosomes decorated by immunosuppressive and tumor-growth promoting proteins. TAM-derived exosomes disseminate these proteins in the tumor microenvironment (TME) promoting tumor cell migration and proliferation. Mechanisms underlying the promotion of glioblastoma growth involved Arginase-1+ exosomes produced by the reprogrammed TAMs. A selective Arginase-1 inhibitor, nor-NOHA reversed growth-promoting effects of Arginase-1 carried by TAM-derived exosomes. The data suggest that GBex-reprogrammed Arginase-1+ TAMs emerge as a major source of exosomes promoting tumor growth and as a potential therapeutic target in glioblastoma.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Azambuja JH,Ludwig N,Yerneni SS,Braganhol E,Whiteside TLdoi
10.3390/ijms21113990subject
Has Abstractpub_date
2020-06-02 00:00:00issue
11issn
1422-0067pii
ijms21113990journal_volume
21pub_type
杂志文章abstract：:Background-It is recognized that inflammation is an underlying cause of dry eye disease (DED), with cytokine release involved. We systematically reviewed literature with meta-analyses to quantitatively summarize the levels of tear cytokines in DED. Methods-The PubMed, Embase, Web of Science, Ovid, Cochrane, and Scopus...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21093111
更新日期：2020-04-28 00:00:00
abstract：:In vitro chondrogenically differentiated mesenchymal stem cells (MSCs) have a tendency to undergo hypertrophy, mirroring the fate of transient "chondrocytes" in the growth plate. As hypertrophy would result in ossification, this fact limits their use in cartilage tissue engineering applications. During limb developmen...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041444
更新日期：2020-02-20 00:00:00
abstract：:Oral biofilms attach onto both teeth surfaces and dental material surfaces in oral cavities. In the meantime, oral biofilms are not only the pathogenesis of dental caries and periodontitis, but also secondary caries and peri-implantitis, which would lead to the failure of clinical treatments. The material surfaces exp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19103157
更新日期：2018-10-14 00:00:00
abstract：:The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many Lactobacillus species, was identi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010091
更新日期：2019-12-21 00:00:00
abstract：:CX3CL1 can function as both an adhesion molecule and a chemokine for CX3CR1+ cells, such as T cells, monocytes, and NK cells. Recent studies have demonstrated that CX3CL1-CX3CR1 interaction is associated with the development of various inflammatory skin diseases. In this study, we examined CX3CR1 involvement in 2,4-di...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197401
更新日期：2020-10-07 00:00:00
abstract：:The plant-specific WUSCHEL-related homeobox (WOX) transcription factor gene family is important for plant growth and development but little studied in oil crops. We identified and characterized 58 putative WOX genes in Brassica napus (BnWOXs), which were divided into three major clades and nine subclades based on the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19113470
更新日期：2018-11-05 00:00:00
abstract：:In the present study, we investigated the signal transduction of mutants of the eel follicle-stimulating hormone receptor (eelFSHR). Specifically, we examined the constitutively activating mutant D540G in the third intracellular loop, and four inactivating mutants (A193V, N195I, R546C, and A548V). To directly assess f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197075
更新日期：2020-09-25 00:00:00
abstract：:Flower development is a vital developmental process in the life cycle of woody perennials, especially fruit trees. Herein, we used transcriptomic, proteomic, and hormone analyses to investigate the key candidate genes/proteins in loquat (Eriobotrya japonica) at the stages of flower bud differentiation (FBD), floral bu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21145107
更新日期：2020-07-20 00:00:00
abstract：:Neoplastic growth and cellular differentiation are critical hallmarks of tumor development. It is well established that cell-to-cell communication between tumor cells and "normal" surrounding cells regulates tumor differentiation and proliferation, aggressiveness, and resistance to treatment. Nevertheless, the mechani...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19051270
更新日期：2018-04-24 00:00:00
abstract：:Lymphoma, a group of widely prevalent hematological malignancies of lymphocyte origin, has become the focus of significant clinical research due to their high propensity for refractory/relapsed (R/R) disease, leading to poor prognostic outcomes. The complex molecular circuitry in lymphomas, especially in the aggressiv...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21030904
更新日期：2020-01-30 00:00:00
abstract：:Many systems, including polymers and molecular liquids, when adequately cooled and/or compressed, solidify into a disordered solid, i.e., a glass. The transition is not abrupt, featuring progressive decrease of the microscopic mobility and huge slowing down of the relaxation. A distinctive aspect of glass-forming mate...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20225708
更新日期：2019-11-14 00:00:00
abstract：:Cystic fibrosis (CF) is caused by mutations in the gene encoding the transmembrane conductance regulator (CFTR) protein. Some CF patients are compound heterozygous or homozygous for nonsense mutations in the CFTR gene. This implies the presence in the transcript of premature termination codons (PTCs) responsible for a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134781
更新日期：2020-07-06 00:00:00
abstract：:Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies, and thus better understanding of its molecular pathology is crucial for us to devise more effective treatment of this deadly disease. As cancer cell line remains a convenient starting point for discovery and proof-of-concept studies, here ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184473
更新日期：2019-09-10 00:00:00
abstract：:In vivo inoculation of cells such as tumor cells and induced pluripotent stem (iPS)/embryonic stem (ES) cells into immunocompromised mice has been considered as a powerful technique to evaluate their potential to proliferate or differentiate into various cell types originating from three germ cell layers. Subcutaneous...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18081678
更新日期：2017-08-02 00:00:00
abstract：:Much of the experimental data, especially in life sciences, is considered to be useless if it demonstrates a large standard deviation from the mean value. The Renaissance distribution, as presented in this study, allows one to extract true values from such statistical data with large noise. To obtain proof of the Rena...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20133250
更新日期：2019-07-02 00:00:00
abstract：:Bovine immunoglobulin G (bIgG) was previously shown to enhance innate immune responses to toll-like receptor (TLR) stimulation, via induction of trained immunity. In this study, we investigated whether minimally processed dairy streams with high levels of whey proteins as potential infant nutrition ingredients could a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21239077
更新日期：2020-11-28 00:00:00
abstract：:The applications of ZnO nanoparticles in agriculture have largely contributed to crop growth regulation, quality enhancement, and induction of stress tolerance, while the underlying mechanisms remain elusive. Herein, the involvement of melatonin synthesis and metabolism in the process of nano-ZnO induced drought toler...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21030782
更新日期：2020-01-25 00:00:00
abstract：:Cadmium is a deleterious environmental pollutant that threats both animals and human health. Oxidative stress and elevated levels of reactive oxygen species (ROS) have recently been reported to be the main cause of cellular damage as a result of cadmium exposure. We investigate, here, the protective effect of strawber...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18050957
更新日期：2017-05-05 00:00:00
abstract：:Demands for safe depigmentation compounds are constantly increasing in the pharmaceutical and cosmetic industry, since the numerous relevant compounds reported to date have shown undesirable side effects or low anti-melanogenic effects. In this study, we reported three novel inhibitors of tyrosinase, which is the key ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21093144
更新日期：2020-04-29 00:00:00
abstract：:Before utilization in biomedical diagnosis, therapeutic treatment, and biotechnology, the diverse variety of peptides and proteins must be preliminarily purified and thoroughly characterized. The recombinant DNA technology and heterologous protein expression have helped simplify the isolation of targeted polypeptides ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21030928
更新日期：2020-01-31 00:00:00
abstract：:Alzheimer's disease (AD) is characterized by extracellular amyloid plaques composed of the β-amyloid peptides and intracellular neurofibrillary tangles and associates with progressive declines in memory and cognition. Several genes play important roles and regulate enzymes that produce a pathological accumulation of β...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21041327
更新日期：2020-02-16 00:00:00
abstract：:Aromatic isocyanides including isocyanonaphthalene derivatives have been proven to be very effective fluorescent sensors for the quantification of Hg(II) ions in water. Thus, the reaction of 1,5-isocyanoaminonaphthalene (1,5-ICAN), which is one of the most important members of this family, with water and HgCl2 as the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21155588
更新日期：2020-08-04 00:00:00
abstract：:Human serum albumin (HSA) is a target for reactive oxygen species (ROS), and alterations of its physiological functions caused by oxidation is a current issue. In this work, the amino-acid residues Trp-214 and Lys-199, which are located at site I of HSA, were experimentally and computationally oxidized, and the effect...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19102868
更新日期：2018-09-21 00:00:00
abstract：:GM2 gangliosidoses are a group of pathologies characterized by GM2 ganglioside accumulation into the lysosome due to mutations on the genes encoding for the β-hexosaminidases subunits or the GM2 activator protein. Three GM2 gangliosidoses have been described: Tay-Sachs disease, Sandhoff disease, and the AB variant. Ce...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21176213
更新日期：2020-08-27 00:00:00
abstract：:Mechanical force is a key factor for the maintenance, adaptation, and function of tendons. Investigating the impact of mechanical loading in tenocytes and tendons might provide important information on in vivo tendon mechanobiology. Therefore, the study aimed at understanding if an in vitro loading set up of tenocytes...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041313
更新日期：2020-02-15 00:00:00
abstract：:The mosquito-borne viral disease caused by the Dengue virus is an expanding global threat. Diagnosis in low-resource-settings and epidemiological surveillance urgently requires new immunoprobes for serological tests. Structure-based epitope prediction is an efficient method to design diagnostic peptidic probes able to...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081921
更新日期：2019-04-18 00:00:00
abstract：:Genomic instability is a hallmark of human cancer and an enabling factor for the genetic alterations that drive cancer development. The processes involved in genomic instability resemble those of meiosis, where genetic material is interchanged between homologous chromosomes. In most types of human cancer, epigenetic c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17060890
更新日期：2016-06-06 00:00:00
abstract：:According to recent estimates, 2%-15% of couples are sterile, and approximately half of the infertility cases are attributed to male reproductive factors. However, the reasons remain undefined in approximately 25% of male infertility cases, and most infertility cases exhibit spermatogenic defects. Numerous genes invol...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms18010097
更新日期：2017-01-05 00:00:00
abstract：:Hyperpolarization-activated cationic HCN channels comprise four members (HCN1-4) that control dendritic integration, synaptic transmission and action potential firing. In the kidney, HCN1, HCN2 and HCN3 are differentially expressed and contribute to the transport of sodium, potassium (K+) and ammonium into the nephron...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms20204995
更新日期：2019-10-09 00:00:00
abstract：:The l-arginine/agmatine transporter AdiC is a prokaryotic member of the SLC7 family, which enables pathogenic enterobacteria to survive the extremely acidic gastric environment. Wild-type AdiC from Escherichia coli, as well as its previously reported point mutants N22A and S26A, were overexpressed homologously and pur...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030918
更新日期：2018-03-20 00:00:00
