In Vivo and In Vitro Mechanical Loading of Mouse Achilles Tendons and Tenocytes-A Pilot Study.


:Mechanical force is a key factor for the maintenance, adaptation, and function of tendons. Investigating the impact of mechanical loading in tenocytes and tendons might provide important information on in vivo tendon mechanobiology. Therefore, the study aimed at understanding if an in vitro loading set up of tenocytes leads to similar regulations of cell shape and gene expression, as loading of the Achilles tendon in an in vivo mouse model. In vivo: The left tibiae of mice (n = 12) were subject to axial cyclic compressive loading for 3 weeks, and the Achilles tendons were harvested. The right tibiae served as the internal non-loaded control. In vitro: tenocytes were isolated from mice Achilles tendons and were loaded for 4 h or 5 days (n = 6 per group) based on the in vivo protocol. Histology showed significant differences in the cell shape between in vivo and in vitro loading. On the molecular level, quantitative real-time PCR revealed significant differences in the gene expression of collagen type I and III and of the matrix metalloproteinases (MMP). Tendon-associated markers showed a similar expression profile. This study showed that the gene expression of tendon markers was similar, whereas significant changes in the expression of extracellular matrix (ECM) related genes were detected between in vivo and in vitro loading. This first pilot study is important for understanding to which extent in vitro stimulation set-ups of tenocytes can mimic in vivo characteristics.


Int J Mol Sci


Fleischhacker V,Klatte-Schulz F,Minkwitz S,Schmock A,Rummler M,Seliger A,Willie BM,Wildemann B




Has Abstract


2020-02-15 00:00:00










  • Functional Identification of the Dextransucrase Gene of Leuconostoc mesenteroides DRP105.

    abstract::Leuconostoc mesenteroides DRP105 isolated from Chinese sauerkraut juice is an intensive producer of dextran. We report the complete genome sequence of Leu. mesenteroides DRP105. This strain contains a dextransucrase gene (dsr) involved in the production of dextran, possibly composed of glucose monomers. To explore the...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Du R,Zhou Z,Han Y

    更新日期:2020-09-09 00:00:00

  • The Impact of the 'Mis-Peptidome' on HLA Class I-Mediated Diseases: Contribution of ERAP1 and ERAP2 and Effects on the Immune Response.

    abstract::The strong association with the Major Histocompatibility Complex (MHC) class I genes represents a shared trait for a group of autoimmune/autoinflammatory disorders having in common immunopathogenetic basis as well as clinical features. Accordingly, the main risk factors for Ankylosing Spondylitis (AS), prototype of th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Tedeschi V,Paldino G,Paladini F,Mattorre B,Tuosto L,Sorrentino R,Fiorillo MT

    更新日期:2020-12-17 00:00:00

  • Bone Marrow Aspirate Concentrate for the Treatment of Avascular Meniscus Tears in a One-Step Procedure-Evaluation of an In Vivo Model.

    abstract::Avascular meniscus tears show poor intrinsic regenerative potential. Thus, lesions within this area predispose the patient to developing knee osteoarthritis. Current research focuses on regenerative approaches using growth factors or mesenchymal stem cells (MSCs) to enhance healing capacity within the avascular menisc...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Koch M,Hammer S,Fuellerer J,Lang S,Pfeifer CG,Pattappa G,Weber J,Loibl M,Nerlich M,Angele P,Zellner J

    更新日期:2019-03-05 00:00:00

  • Dexamethasone Loaded Liposomes by Thin-Film Hydration and Microfluidic Procedures: Formulation Challenges.

    abstract::Liposomes have been one of the most exploited drug delivery systems in recent decades. However, their large-scale production with low batch-to-batch differences is a challenge for industry, which ultimately delays the clinical translation of new products. We have investigated the effects of formulation parameters on t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Al-Amin MD,Bellato F,Mastrotto F,Garofalo M,Malfanti A,Salmaso S,Caliceti P

    更新日期:2020-02-26 00:00:00

  • Consecutive Isoproterenol and Adenosine Treatment Confers Marked Protection against Reperfusion Injury in Adult but Not in Immature Heart: A Role for Glycogen.

    abstract::Consecutive treatment of adult rat heart with isoproterenol and adenosine (Iso/Aden), known to consecutively activate PKA/PKC signaling, is cardioprotective against ischemia and reperfusion (I/R). Whether this is cardioprotective in an immature heart is unknown. Langendorff-perfused hearts from adult and immature (60 ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lewis M,Szobi A,Balaska D,Khaliulin I,Adameova A,Griffiths E,Orchard CH,Suleiman MS

    更新日期:2018-02-07 00:00:00

  • Identification of tillering node proteins differentially accumulated in barley recombinant inbred lines with different juvenile growth habits.

    abstract::Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kuczyńska A,Kosmala A,Surma M,Adamski T

    更新日期:2012-01-01 00:00:00

  • Vitamin D in Triple-Negative and BRCA1-Deficient Breast Cancer-Implications for Pathogenesis and Therapy.

    abstract::Several studies show that triple-negative breast cancer (TNBC) patients have the lowest vitamin D concentration among all breast cancer types, suggesting that this vitamin may induce a protective effect against TNBC. This effect of the active metabolite of vitamin D, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D), can be attr...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Blasiak J,Pawlowska E,Chojnacki J,Szczepanska J,Fila M,Chojnacki C

    更新日期:2020-05-23 00:00:00

  • Non-Coding RNAs in Cancer: An Interview with Dr. Martin Pichler.

    abstract::In this issue, we are pleased and honored to have an interview with Professor Martin Pichler, who is the Collection Editor for the International Journal of Molecular Sciences Topical Collection of "Regulation by Non-Coding RNAs" [1].[...]. ...

    journal_title:International journal of molecular sciences

    pub_type: 社论,面试


    authors: International Journal Of Molecular Sciences Editorial Office.

    更新日期:2016-04-21 00:00:00

  • Role of cytokine signaling during nervous system development.

    abstract::Cytokines are signaling proteins that were first characterized as components of the immune response, but have been found to have pleiotropic effects in diverse aspects of body function in health and disease. They are secreted by numerous cells and are used extensively in intercellular communications to produce differe...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Mousa A,Bakhiet M

    更新日期:2013-07-04 00:00:00

  • Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation.

    abstract::Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this study, we identify abundant proteins in the formation of the biofilm of a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Moreno-García J,Mauricio JC,Moreno J,García-Martínez T

    更新日期:2017-03-28 00:00:00

  • Normal FGF-21-Serum Levels in Patients with Carnitine Palmitoyltransferase II (CPT II) Deficiency.

    abstract::Fibroblast growth factor 21 (FGF-21) is known to be a biomarker for mitochondrial disorders. An upregulation of FGF-21 in serum and muscle of carnitine palmitoyltransferase I (CPT I) and carnitine palmitoyltransferase II (CPT II) knock-out mice has been reported. In human CPT II deficiency, enzyme activity and protein...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Motlagh Scholle L,Lehmann D,Joshi PR,Zierz S

    更新日期:2019-03-20 00:00:00

  • Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in Gossypium hirsutum.

    abstract::Chemical defoliation is an important part of cotton mechanical harvesting, which can effectively reduce the impurity content. Thidiazuron (TDZ) is the most used chemical defoliant on cotton. To better clarify the mechanism of TDZ promoting cotton leaf abscission, a greenhouse experiment was conducted on two cotton cul...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Jin D,Wang X,Xu Y,Gui H,Zhang H,Dong Q,Sikder RK,Yang G,Song M

    更新日期:2020-04-15 00:00:00

  • Comparative Small RNA Analysis of Pollen Development in Autotetraploid and Diploid Rice.

    abstract::MicroRNAs (miRNAs) play key roles in plant reproduction. However, knowledge on microRNAome analysis in autotetraploid rice is rather limited. Here, high-throughput sequencing technology was employed to analyze miRNAomes during pollen development in diploid and polyploid rice. A total of 172 differentially expressed mi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li X,Shahid MQ,Wu J,Wang L,Liu X,Lu Y

    更新日期:2016-04-12 00:00:00

  • Identification, Characterization and Synthesis of Walterospermin, a Sperm Motility Activator from the Egyptian Black Snake Walterinnesia aegyptia Venom.

    abstract::Animal venoms are small natural mixtures highly enriched in bioactive components. They are known to target at least two important pharmacological classes of cell surface receptors: ion channels and G protein coupled receptors. Since sperm cells express a wide variety of ion channels and membrane receptors, required fo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Abd El-Aziz TM,Jaquillard L,Bourgoin-Voillard S,Martinez G,Triquigneaux M,Zoukimian C,Combemale S,Hograindleur JP,Al Khoury S,Escoffier J,Michelland S,Bulet P,Beroud R,Seve M,Arnoult C,De Waard M

    更新日期:2020-10-21 00:00:00

  • Role of the Novel Hsp90 Co-Chaperones in Dynein Arms' Preassembly.

    abstract::The outer and inner dynein arms (ODAs and IDAs) are composed of multiple subunits including dynein heavy chains possessing a motor domain. These complex structures are preassembled in the cytoplasm before being transported to the cilia. The molecular mechanism(s) controlling dynein arms' preassembly is poorly understo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Fabczak H,Osinka A

    更新日期:2019-12-07 00:00:00

  • Homogenized finite element analysis on effective elastoplastic mechanical behaviors of composite with imperfect interfaces.

    abstract::A three-dimensional (3D) representative volume element (RVE) model was developed for analyzing effective mechanical behavior of fiber-reinforced ceramic matrix composites with imperfect interfaces. In the model, the fiber is assumed to be perfectly elastic until its tensile strength, and the ceramic material is modele...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Jiang WG,Zhong RZ,Qin QH,Tong YG

    更新日期:2014-12-16 00:00:00

  • Proteome and peptidome of human acquired enamel pellicle on deciduous teeth.

    abstract::Understanding the composition and structure of the acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than 100 proteins from AEP formed o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zimmerman JN,Custodio W,Hatibovic-Kofman S,Lee YH,Xiao Y,Siqueira WL

    更新日期:2013-01-07 00:00:00

  • A Novel Selective 11β-HSD1 Inhibitor, (E)-4-(2-(6-(2,6-Dichloro-4-(Trifluoromethyl)Phenyl)-4-Methyl-1,1-Dioxido-1,2,6-Thiadiazinan-2-yl)Acetamido)Adamantan-1-Carboxamide (KR-67607), Prevents BAC-Induced Dry Eye Syndrome.

    abstract::Dry eye syndrome is the most common eye disease and it is caused by various reasons. As the balance of the tear film that protects the eyes is broken due to various causes, it becomes impossible to properly protect the eyes. In this study, the protective effects and underlying mechanisms of topical (E)-4-(2-(6-(2,6-di...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Na YJ,Choi KJ,Jung WH,Park SB,Kang S,Ahn JH,Kim KY

    更新日期:2020-05-25 00:00:00

  • Characterization of Fluid Biomarkers Reveals Lysosome Dysfunction and Neurodegeneration in Neuronopathic MPS II Patients.

    abstract::Mucopolysaccharidosis type II is a lysosomal storage disorder caused by a deficiency of iduronate-2-sulfatase (IDS) and characterized by the accumulation of the primary storage substrate, glycosaminoglycans (GAGs). Understanding central nervous system (CNS) pathophysiology in neuronopathic MPS II (nMPS II) has been hi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Bhalla A,Ravi R,Fang M,Arguello A,Davis SS,Chiu CL,Blumenfeld JR,Nguyen HN,Earr TK,Wang J,Astarita G,Zhu Y,Fiore D,Scearce-Levie K,Diaz D,Cahan H,Troyer MD,Harris JM,Escolar ML

    更新日期:2020-07-22 00:00:00

  • Biochemical and Behavioral Consequences of Ethanol Intake in a Mouse Model of Metabolic Syndrome.

    abstract::Ethanol abuse is a common issue in individuals with sedentary lifestyles, unbalanced diets, and metabolic syndrome. Both ethanol abuse and metabolic syndrome have negative impacts on the central nervous system, with effects including cognitive impairment and brain oxidative status deterioration. The combined effects o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Baliño P,Romero-Cano R,Muriach M

    更新日期:2021-01-15 00:00:00

  • The Roles of Sodium-Independent Inorganic Phosphate Transporters in Inorganic Phosphate Homeostasis and in Cancer and Other Diseases.

    abstract::Inorganic phosphate (Pi) is an essential nutrient for the maintenance of cells. In healthy mammals, extracellular Pi is maintained within a narrow concentration range of 0.70 to 1.55 mM. Mammalian cells depend on Na+/Pi cotransporters for Pi absorption, which have been well studied. However, a new type of sodium-indep...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Lacerda-Abreu MA,Russo-Abrahão T,Meyer-Fernandes JR

    更新日期:2020-12-06 00:00:00

  • Insect Cecropins, Antimicrobial Peptides with Potential Therapeutic Applications.

    abstract::The alarming escalation of infectious diseases resistant to conventional antibiotics requires urgent global actions, including the development of new therapeutics. Antimicrobial peptides (AMPs) represent potential alternatives in the treatment of multi-drug resistant (MDR) infections. Here, we focus on Cecropins (Cecs...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Brady D,Grapputo A,Romoli O,Sandrelli F

    更新日期:2019-11-22 00:00:00

  • Three-Dimensional Electrodes for High-Performance Bioelectrochemical Systems.

    abstract::Bioelectrochemical systems (BES) are groups of bioelectrochemical technologies and platforms that could facilitate versatile environmental and biological applications. The performance of BES is mainly determined by the key process of electron transfer at the bacteria and electrode interface, which is known as extracel...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Yu YY,Zhai DD,Si RW,Sun JZ,Liu X,Yong YC

    更新日期:2017-01-04 00:00:00

  • Hsa-let-7g miRNA targets caspase-3 and inhibits the apoptosis induced by ox-LDL in endothelial cells.

    abstract::It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovere...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zhang Y,Chen N,Zhang J,Tong Y

    更新日期:2013-11-18 00:00:00

  • LARP6 Meets Collagen mRNA: Specific Regulation of Type I Collagen Expression.

    abstract::Type I collagen is the most abundant structural protein in all vertebrates, but its constitutive rate of synthesis is low due to long half-life of the protein (60-70 days). However, several hundred fold increased production of type I collagen is often seen in reparative or reactive fibrosis. The mechanism which is res...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zhang Y,Stefanovic B

    更新日期:2016-03-22 00:00:00

  • Syntenin: PDZ Protein Regulating Signaling Pathways and Cellular Functions.

    abstract::Syntenin is an adaptor-like molecule that has two adjacent tandem postsynaptic density protein 95/Discs large protein/Zonula occludens 1 (PDZ) domains. The PDZ domains of syntenin recognize multiple peptide motifs with low to moderate affinity. Many reports have indicated interactions between syntenin and a plethora o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Shimada T,Yasuda S,Sugiura H,Yamagata K

    更新日期:2019-08-26 00:00:00

  • Regulation of Autophagic Signaling by Mechanical Loading and Inflammation in Human PDL Fibroblasts.

    abstract::Autophagy (cellular self-consumption) is a crucial adaptation mechanism during cellular stress conditions. This study aimed to examine how this important process is regulated in human periodontal ligament (PDL) fibroblasts by mechanical and inflammatory stress conditions and whether the mammalian target of rapamycin (...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Blawat K,Mayr A,Hardt M,Kirschneck C,Nokhbehsaim M,Behl C,Deschner J,Jäger A,Memmert S

    更新日期:2020-12-11 00:00:00

  • Characterization and Immobilization of a Novel SGNH Family Esterase (LaSGNH1) from Lactobacillus acidophilus NCFM.

    abstract::The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many Lactobacillus species, was identi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Le LTHL,Yoo W,Jeon S,Kim KK,Kim TD

    更新日期:2019-12-21 00:00:00

  • MiR-30a-5p Inhibits Epithelial-to-Mesenchymal Transition and Upregulates Expression of Tight Junction Protein Claudin-5 in Human Upper Tract Urothelial Carcinoma Cells.

    abstract::The involvement of microRNAs (miRNAs) in cancer development and their potential as prognostic biomarkers are becoming increasingly known. However, the signature of miRNAs and their regulatory roles in tumorigenesis of upper tract urothelial carcinoma (UTUC) remain to be elucidated. This study aimed to profile the miRN...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Chung YH,Li SC,Kao YH,Luo HL,Cheng YT,Lin PR,Tai MH,Chiang PH

    更新日期:2017-08-22 00:00:00

  • lncRNA_Mdeep: An Alignment-Free Predictor for Distinguishing Long Non-Coding RNAs from Protein-Coding Transcripts by Multimodal Deep Learning.

    abstract::Long non-coding RNAs (lncRNAs) play crucial roles in diverse biological processes and human complex diseases. Distinguishing lncRNAs from protein-coding transcripts is a fundamental step for analyzing the lncRNA functional mechanism. However, the experimental identification of lncRNAs is expensive and time-consuming. ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Fan XN,Zhang SW,Zhang SY,Ni JJ

    更新日期:2020-07-23 00:00:00