Abstract：
:Deregulated microRNA (miRNA) expression profiles and their contribution to carcinogenesis have been observed in virtually all types of human cancer. However, their role in the pathogenesis of rare mesenchymal gastrointestinal stromal tumors (GISTs) is not well defined, yet. In this study, we aimed to investigate the role of two miRNAs strongly downregulated in GIST-miR-375-3p and miR-200b-3p-in the pathogenesis of GIST. To achieve this, miRNA mimics were transfected into GIST-T1 cells and changes in the potential target gene mRNA and protein expression, as well as alterations in cell viability, migration, apoptotic cell counts and direct miRNA-target interaction, were evaluated. Results revealed that overexpression of miR-375-3p downregulated the expression of KIT mRNA and protein by direct binding to KIT 3'UTR, reduced GIST cell viability and migration rates. MiR-200b-3p lowered expression of ETV1 protein, directly targeted and lowered expression of EGFR mRNA and protein, and negatively affected cell migration rates. To conclude, the present study identified that miR-375-3p and miR-200b-3p have a tumor-suppressive role in GIST.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Gyvyte U,Lukosevicius R,Inciuraite R,Streleckiene G,Gudoityte G,Bekampyte J,Valentini S,Salteniene V,Ruzgys P,Satkauskas S,Zviniene K,Kupcinskas J,Skieceviciene Jdoi
10.3390/ijms21145151subject
Has Abstractpub_date
2020-07-21 00:00:00issue
14issn
1422-0067pii
ijms21145151journal_volume
21pub_type
杂志文章abstract：:A series of novel 1-(4-substitutedbenzoyl)-4-(4-chlorobenzhydryl)piperazine derivatives 5a-g was designed by a nucleophilic substitution reaction of 1-(4-chlorobenzhydryl)piperazine with various benzoyl chlorides and characterized by elemental analyses, IR and (1)H nuclear magnetic resonance spectra. Cytotoxicity of t...
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更新日期：2020-03-18 00:00:00
