当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES期刊下所有文献 > Type 2 Diabetes Alters Intracellular Ca2+ Handling in Native Endothelium of Excised Rat Aorta.

Type 2 Diabetes Alters Intracellular Ca2+ Handling in Native Endothelium of Excised Rat Aorta.

Abstract：

:An increase in intracellular Ca2+ concentration ([Ca2+]i) plays a key role in controlling endothelial functions; however, it is still unclear whether endothelial Ca2+ handling is altered by type 2 diabetes mellitus, which results in severe endothelial dysfunction. Herein, we analyzed for the first time the Ca2+ response to the physiological autacoid ATP in native aortic endothelium of obese Zucker diabetic fatty (OZDF) rats and their lean controls, which are termed LZDF rats. By loading the endothelial monolayer with the Ca2+-sensitive fluorophore, Fura-2/AM, we found that the endothelial Ca2+ response to 20 µM and 300 µM ATP exhibited a higher plateau, a larger area under the curve and prolonged duration in OZDF rats. The "Ca2+ add-back" protocol revealed no difference in the inositol-1,4,5-trisphosphate-releasable endoplasmic reticulum (ER) Ca2+ pool, while store-operated Ca2+ entry was surprisingly down-regulated in OZDF aortae. Pharmacological manipulation disclosed that sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) activity was down-regulated by reactive oxygen species in native aortic endothelium of OZDF rats, thereby exaggerating the Ca2+ response to high agonist concentrations. These findings shed new light on the mechanisms by which type 2 diabetes mellitus may cause endothelial dysfunction by remodeling the intracellular Ca2+ toolkit.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Berra-Romani R,Guzmán-Silva A,Vargaz-Guadarrama A,Flores-Alonso JC,Alonso-Romero J,Treviño S,Sánchez-Gómez J,Coyotl-Santiago N,García-Carrasco M,Moccia F

doi

10.3390/ijms21010250

subject

Has Abstract

pub_date

2019-12-30 00:00:00

issue

1

issn

1422-0067

pii

ijms21010250

journal_volume

21

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES文献大全
  • Nanoparticles and Colloidal Hydrogels of Chitosan-Caseinate Polyelectrolyte Complexes for Drug-Controlled Release Applications.

    abstract：:Chitosan-caseinate nanoparticles were synthesized by polyelectrolyte complex (PEC) formation. Caseinate is an anionic micellar nanocolloid in aqueous solutions, which association with the polycationic chitosan yielded polyelectrolyte complexes with caseinate cores surrounded by a chitosan corona. The pre-structuration...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21165602

    authors： Lall A,Kamdem Tamo A,Doench I,David L,Nunes de Oliveira P,Gorzelanny C,Osorio-Madrazo A

    更新日期：2020-08-05 00:00:00

  • Mitochondrial Quality Control in Age-Related Pulmonary Fibrosis.

    abstract：:Idiopathic pulmonary fibrosis (IPF) is age-related interstitial lung disease of unknown etiology. About 100,000 people in the U.S have IPF, with a 3-year median life expectancy post-diagnosis. The development of an effective treatment for pulmonary fibrosis will require an improved understanding of its molecular patho...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21020643

    authors： Roque W,Cuevas-Mora K,Romero F

    更新日期：2020-01-18 00:00:00

  • CYP3A5 Genotype as a Potential Pharmacodynamic Biomarker for Tacrolimus Therapy in Ulcerative Colitis in Japanese Patients.

    abstract：:Tacrolimus has been used to induce remission in patients with steroid-refractory ulcerative colitis. It poses a problem of large individual differences in dosage necessary to attain target blood concentration and, often, this leads to drug inefficacy. We examined the difference in mRNA expression levels of ATP binding...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21124347

    authors： Yamamoto Y,Nakase H,Matsuura M,Maruyama S,Masuda S

    更新日期：2020-06-18 00:00:00

  • Functional Mechanisms of Mitochondrial Respiratory Chain Supercomplex Assembly Factors and Their Involvement in Muscle Quality.

    abstract：:Impairment of skeletal muscle function causes disabilities in elderly people. Therefore, in an aged society, prevention and treatment of sarcopenia are important for expanding healthy life expectancy. In addition to aging, adipose tissue disfunction and inflammation also contribute to the pathogenesis of sarcopenia by...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21093182

    authors： Azuma K,Ikeda K,Inoue S

    更新日期：2020-04-30 00:00:00

  • Programmed Cell Death in the Pathogenesis of Influenza.

    abstract：:Influenza is a respiratory disease induced by infection by the influenza virus, which is a member of Orthomyxoviridae family. This infectious disease has serious impacts on public health systems and results in considerable mortality and economic costs throughout the world. Based on several experimental studies, massiv...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19072065

    authors： Fujikura D,Miyazaki T

    更新日期：2018-07-16 00:00:00

  • Genome-Wide Identification and Characterization of the UBP Gene Family in Moso Bamboo (Phyllostachys edulis).

    abstract：:The largest group of deubiquitinases-ubiquitin-specific proteases (UBPs)-perform extensive and significant roles in plants, including the regulation of development and stress responses. A comprehensive analysis of UBP genes has been performed in Arabidopsis thaliana, but no systematic study has been conducted in moso ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20174309

    authors： Wu R,Shi Y,Zhang Q,Zheng W,Chen S,Du L,Lu C

    更新日期：2019-09-03 00:00:00

  • GABA-Alleviated Oxidative Injury Induced by Salinity, Osmotic Stress and their Combination by Regulating Cellular and Molecular Signals in Rice.

    abstract：:This study was conducted in order to determine the effect of priming with γ-aminobutyric acid (GABA) at 0.5 mM on rice (Oryza sativa L.) seed germination under osmotic stress (OS) induced by polyethylene glycol (30 g/L PEG 6000); and salinity stress (S, 150 mM NaCl) and their combination (OS+S). Priming with GABA sign...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20225709

    authors： Sheteiwy MS,Shao H,Qi W,Hamoud YA,Shaghaleh H,Khan NU,Yang R,Tang B

    更新日期：2019-11-14 00:00:00

  • TGF-β-Dependent Growth Arrest and Cell Migration in Benign and Malignant Breast Epithelial Cells Are Antagonistically Controlled by Rac1 and Rac1b.

    abstract：:Despite improvements in diagnosis and treatment, breast cancer is still the most common cancer type among non-smoking females. TGF-β can inhibit breast cancer development by inducing cell cycle arrest in both, cancer cells and, as part of a senescence program in normal human mammary epithelial cells (HMEC). Moreover, ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms18071574

    authors： Melzer C,von der Ohe J,Hass R,Ungefroren H

    更新日期：2017-07-20 00:00:00

  • Role of Major Endocannabinoid-Binding Receptors during Mouse Oocyte Maturation.

    abstract：:Endocannabinoids are key-players of female fertility and potential biomarkers of reproductive dysfunctions. Here, we investigated localization and expression of cannabinoid receptor type-1 and -2 (CB1R and CB2R), G-protein coupled receptor 55 (GPR55), and transient receptor potential vanilloid type 1 channel (TRPV1) i...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20122866

    authors： Cecconi S,Rossi G,Oddi S,Di Nisio V,Maccarrone M

    更新日期：2019-06-12 00:00:00

  • Microbiological, Physicochemical, and Immunological Analysis of a Commercial Cashew Nut-Based Yogurt.

    abstract：:Nut-based milks and yogurts are gaining popularity, but may not offer the same benefits as dairy yogurts to consumers. Cashew nuts often cause severe allergic reactions, and cashew nut allergens are stable to several types of processing. To compare its characteristics to dairy yogurt and characterize the effects of fe...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21218267

    authors： Mattison CP,Aryana KJ,Clermont K,Prestenburg E,Lloyd SW,Grimm CC,Wasserman RL

    更新日期：2020-11-04 00:00:00

  • Pro-apoptotic effect of rice bran inositol hexaphosphate (IP6) on HT-29 colorectal cancer cells.

    abstract：:Inositol hexaphosphate (IP6), or phytic acid is a natural dietary ingredient and has been described as a "natural cancer fighter", being an essential component of nutritional diets. The marked anti-cancer effect of IP6 has resulted in our quest for an understanding of its mechanism of action. In particular, our data p...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms141223545

    authors： Shafie NH,Esa NM,Ithnin H,Saad N,Pandurangan AK

    更新日期：2013-12-02 00:00:00

  • The Effect of the Chorion on Size-Dependent Acute Toxicity and Underlying Mechanisms of Amine-Modified Silver Nanoparticles in Zebrafish Embryos.

    abstract：:As the worldwide application of nanomaterials in commercial products increases every year, various nanoparticles from industry might present possible risks to aquatic systems and human health. Presently, there are many unknowns about the toxic effects of nanomaterials, especially because the unique physicochemical pro...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21082864

    authors： Chen ZY,Li NJ,Cheng FY,Hsueh JF,Huang CC,Lu FI,Fu TF,Yan SJ,Lee YH,Wang YJ

    更新日期：2020-04-20 00:00:00

  • Antagonistic Autoantibodies to Insulin-Like Growth Factor-1 Receptor Associate with Poor Physical Strength.

    abstract：:Natural autoantibodies to the IGF1 receptor (IGF1R-aAb) have been described in relation to Graves' ophthalmopathy. Other physiological roles of natural IGF1R-aAb are not known. We hypothesized that IGF1R-aAb may be related to muscle development. Serum samples (n = 408) from young overweight subjects (n = 143) were col...

    journal_title：International journal of molecular sciences

    pub_type： 临床试验,杂志文章

    doi：10.3390/ijms21020463

    authors： Schwiebert C,Kühnen P,Becker NP,Welsink T,Keller T,Minich WB,Wiegand S,Schomburg L

    更新日期：2020-01-11 00:00:00

  • Programmed Cell Death in the Left and Right Ventricle of the Late Phase of Post-Infarction Heart Failure.

    abstract：:While necroptosis has been shown to contribute to the pathogenesis of post-infarction heart failure (HF), the role of autophagy remains unclear. Likewise, linkage between these two cell death modalities has not been sufficiently investigated. HF was induced by 60-min left coronary occlusion in adult Wistar rats and he...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21207782

    authors： Lichý M,Szobi A,Hrdlička J,Neckář J,Kolář F,Adameová A

    更新日期：2020-10-21 00:00:00

  • Hepatic microRNA Expression by PGC-1α and PGC-1β in the Mouse.

    abstract：:The fine-tuning of liver metabolism is essential to maintain the whole-body homeostasis and to prevent the onset of diseases. The peroxisome proliferator-activated receptor-γ coactivators (PGC-1s) are transcriptional key players of liver metabolism, able to regulate mitochondrial function, gluconeogenesis and lipid me...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20225735

    authors： Piccinin E,Arconzo M,Graziano G,Vacca M,Peres C,Bellafante E,Villani G,Moschetta A

    更新日期：2019-11-15 00:00:00

  • Determination of aflatoxins in peanut products in the northeast region of São Paulo, Brazil.

    abstract：:The aim of the present study was to determine aflatoxin levels in peanut products traded in the Northeast region of São Paulo, Brazil. To this end, 240 samples of peanut products traded in the cities of Araras, Leme, Pirassununga and Porto Ferreira were collected from June 2006 to May 2007. The samples were analyzed f...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms10010174

    authors： Oliveira CA,Gonçalves NB,Rosim RE,Fernandes AM

    更新日期：2009-01-01 00:00:00

  • The Biological Significance and Regulatory Mechanism of c-Myc Binding Protein 1 (MBP-1).

    abstract：:Alternatively translated from the ENO gene and expressed in an array of vertebrate and plant tissues, c-Myc binding protein 1 (MBP-1) participates in the regulation of growth in organisms, their development and their environmental responses. As a transcriptional repressor of multiple proto-oncogenes, vertebrate MBP-1 ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms19123868

    authors： Liu Z,Zhang A,Zheng L,Johnathan AF,Zhang J,Zhang G

    更新日期：2018-12-04 00:00:00

  • Palmitoylethanolamide inhibits glutamate release in rat cerebrocortical nerve terminals.

    abstract：:The effect of palmitoylethanolamide (PEA), an endogenous fatty acid amide displaying neuroprotective actions, on glutamate release from rat cerebrocortical nerve terminals (synaptosomes) was investigated. PEA inhibited the Ca²⁺-dependent release of glutamate, which was triggered by exposing synaptosomes to the potassi...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms16035555

    authors： Lin TY,Lu CW,Wu CC,Huang SK,Wang SJ

    更新日期：2015-03-11 00:00:00

  • Detection of a G-Quadruplex as a Regulatory Element in Thymidylate synthase for Gene Silencing Using Polypurine Reverse Hoogsteen Hairpins.

    abstract：:Thymidylate synthase (TYMS) enzyme is an anti-cancer target given its role in DNA biosynthesis. TYMS inhibitors (e.g., 5-Fluorouracil) can lead to drug resistance through an autoregulatory mechanism of TYMS that causes its overexpression. Since G-quadruplexes (G4) can modulate gene expression, we searched for putative...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21145028

    authors： Aubets E,J Félix A,Garavís M,Reyes L,Aviñó A,Eritja R,Ciudad CJ,Noé V

    更新日期：2020-07-16 00:00:00

  • The potential of liposomes with carbonic anhydrase IX to deliver anticancer ingredients to cancer cells in vivo.

    abstract：:Drug delivery nanocarriers, especially targeted drug delivery by liposomes are emerging as a class of therapeutics for cancer. Early research results suggest that liposomal therapeutics enhanced efficacy, while simultaneously reducing side effects, owing to properties such as more targeted localization in tumors and a...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms16010230

    authors： Ng HL,Lu A,Lin G,Qin L,Yang Z

    更新日期：2014-12-24 00:00:00

  • Soluble Epidermal Growth Factor Receptors (sEGFRs) in Cancer: Biological Aspects and Clinical Relevance.

    abstract：:The identification of molecules that can reliably detect the presence of a tumor or predict its behavior is one of the biggest challenges of research in cancer biology. Biological fluids are intriguing mediums, containing many molecules that express the individual health status and, accordingly, may be useful in estab...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17040593

    authors： Maramotti S,Paci M,Manzotti G,Rapicetta C,Gugnoni M,Galeone C,Cesario A,Lococo F

    更新日期：2016-04-19 00:00:00

  • VDAC1 and the TSPO: Expression, Interactions, and Associated Functions in Health and Disease States.

    abstract：:The translocator protein (TSPO), located at the outer mitochondrial membrane (OMM), serves multiple functions and contributes to numerous processes, including cholesterol import, mitochondrial metabolism, apoptosis, cell proliferation, Ca2+ signaling, oxidative stress, and inflammation. TSPO forms a complex with the v...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms20133348

    authors： Shoshan-Barmatz V,Pittala S,Mizrachi D

    更新日期：2019-07-08 00:00:00

  • Rheumatoid Arthritis: The Stride from Research to Clinical Practice.

    abstract：:Over 70 different genetic variants with a significant association with rheumatoid arthritis (RA) have been discovered. Anti-citrullination protein antibodies (ACPA)-positive RA variants are more well-defined than their ACPA-negative counterparts. The human leukocyte antigen, HLA-DRB1 locus remains the prime suspect in...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms17060900

    authors： Chung IM,Ketharnathan S,Thiruvengadam M,Rajakumar G

    更新日期：2016-06-08 00:00:00

  • Cisplatin protects against acute liver failure by inhibiting nuclear HMGB1 release.

    abstract：:Cisplatin is one of the most widely used chemical drugs for anticancer treatment. Recent studies have focused on the ability of cisplatin to retain the high mobility group box 1 (HMGB1) protein in cisplatin-DNA adducts, thereby preventing its release from the nucleus. Because HMGB1 is a powerful inflammatory mediator ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms140611224

    authors： Li X,Wang LK,Wang LW,Han XQ,Yang F,Gong ZJ

    更新日期：2013-05-27 00:00:00

  • Bio-guided isolation of the cytotoxic terpenoids from the roots of Euphorbia kansui against human normal cell lines L-O2 and GES-1.

    abstract：:The dried roots of Euphorbia kansui (kansui) have been used for centuries in China as a herbal medicine for edema, ascites, and asthma. The 95% ethanol extract showed a significant inhibition of cell proliferation against human normal cell lines L-O2 and GES-1. Bioassay-guided separation of the 95% ethanol extract fro...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms130911247

    authors： Zhang L,Gao L,Li Z,Yan X,Yang Y,Tang Y,Cao Y,Ding A

    更新日期：2012-01-01 00:00:00

  • Overview of Epstein-Barr-Virus-Associated Gastric Cancer Correlated with Prognostic Classification and Development of Therapeutic Options.

    abstract：:Gastric cancer (GC) is a deadly disease with poor prognosis that is characterized by heterogeneity. New classifications based on histologic features, genotypes, and molecular phenotypes, for example, the Cancer Genome Atlas subtypes and those by the Asian Cancer Research Group, help understand the carcinogenic differe...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章,评审

    doi：10.3390/ijms21249400

    authors： Re V,Brisotto G,Repetto O,De Zorzi M,Caggiari L,Zanussi S,Alessandrini L,Canzonieri V,Miolo G,Puglisi F,Belluco C,Steffan A,Cannizzaro R

    更新日期：2020-12-10 00:00:00

  • Structural Diversity and Dynamics of Human Three-Finger Proteins Acting on Nicotinic Acetylcholine Receptors.

    abstract：:Ly-6/uPAR or three-finger proteins (TFPs) contain a disulfide-stabilized β-structural core and three protruding loops (fingers). In mammals, TFPs have been found in epithelium and the nervous, endocrine, reproductive, and immune systems. Here, using heteronuclear NMR, we determined the three-dimensional (3D) structure...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21197280

    authors： Paramonov AS,Kocharovskaya MV,Tsarev AV,Kulbatskii DS,Loktyushov EV,Shulepko MA,Kirpichnikov MP,Lyukmanova EN,Shenkarev ZO

    更新日期：2020-10-01 00:00:00

  • Degenerated Cones in Cultured Human Retinas Can Successfully Be Optogenetically Reactivated.

    abstract：:Biblical references aside, restoring vision to the blind has proven to be a major technical challenge. In recent years, considerable advances have been made towards this end, especially when retinal degeneration underlies the vision loss such as occurs with retinitis pigmentosa. Under these conditions, optogenetic the...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms21020522

    authors： Kamar S,Howlett MHC,Klooster J,Graaff W,Csikós T,Rabelink MJWE,Hoeben RC,Kamermans M

    更新日期：2020-01-14 00:00:00

  • PSMA-Oriented Target Delivery of Novel Anticancer Prodrugs: Design, Synthesis, and Biological Evaluations of Oligopeptide-Camptothecin Conjugates.

    abstract：:Clinical applications of camptothecin (CPT) have been heavily hindered due to its non-targeted toxicity, active lactone ring instability, and poor water solubility. Targeted drug delivery systems may offer the possibility to overcome the above issues as reported. In this research, a series of prostate-specific membran...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms19103251

    authors： Xu B,Zhou F,Yan MM,Cai DS,Guo WB,Yang YQ,Jia XH,Zhang WX,Li T,Ma T,Wang PL,Lei HM

    更新日期：2018-10-19 00:00:00

  • Combined Proteomic and Metabolomic Profiling of the Arabidopsis thaliana vps29 Mutant Reveals Pleiotropic Functions of the Retromer in Seed Development.

    abstract：:The retromer is a multiprotein complex conserved from yeast to humans, which is involved in intracellular protein trafficking and protein recycling. Selection of cargo proteins transported by the retromer depends on the core retromer subunit composed of the three vacuolar protein sorting (VPS) proteins, namely VPS26, ...

    journal_title：International journal of molecular sciences

    pub_type： 杂志文章

    doi：10.3390/ijms20020362

    authors： Durand TC,Cueff G,Godin B,Valot B,Clément G,Gaude T,Rajjou L

    更新日期：2019-01-16 00:00:00