Abstract:
:Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this study, we focus our attention, for the first time, on the marine sponge Ianthella labyrinthus Bergquist & Kelly-Borges, 1995 (Demospongiae: Verongida: Ianthellidae) as a novel potential source of naturally prestructured bandage-like 3D scaffolds which can be isolated simultaneously with biologically active bromotyrosines. Specifically, translucent and elastic flat chitinous scaffolds have been obtained after bromotyrosine extraction and chemical treatments of the sponge skeleton with alternate alkaline and acidic solutions. For the first time, cardiomyocytes differentiated from human induced pluripotent stem cells (iPSC-CMs) have been used to test the suitability of I. labyrinthus chitinous skeleton as ready-to-use scaffold for their cell culture. Results reveal a comparable attachment and growth on isolated chitin-skeleton, compared to scaffolds coated with extracellular matrix mimetic Geltrex®. Thus, the natural, unmodified I. labyrinthus cleaned sponge skeleton can be used to culture iPSC-CMs and 3D tissue engineering. In addition, I. labyrinthus chitin-based scaffolds demonstrate strong and efficient capability to absorb blood deep into the microtubes due to their excellent capillary effect. These findings are suggestive of the future development of new sponge chitin-based absorbable hemostats as alternatives to already well recognized cellulose-based fabrics.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Schubert M,Binnewerg B,Voronkina A,Muzychka L,Wysokowski M,Petrenko I,Kovalchuk V,Tsurkan M,Martinovic R,Bechmann N,Ivanenko VN,Fursov A,Smolii OB,Fromont J,Joseph Y,Bornstein SR,Giovine M,Erpenbeck D,Guan K,Ehrlichdoi
10.3390/ijms20205105subject
Has Abstractpub_date
2019-10-15 00:00:00issue
20issn
1422-0067pii
ijms20205105journal_volume
20pub_type
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