Abstract：
:Wilms tumor protein-1 (WT1) is an attractive target for adoptive T-cell therapy due to its expression in solid tumors and hematologic malignancies. However, T cells recognizing WT1 occur in low frequencies in the peripheral blood of healthy donors, limiting potential therapeutic possibilities. Tin mesoporphyrin (SnMP) is known to inhibit heme oxygenase-1 (HO-1), which has been shown to boost the activation and proliferation of human virus-specific T cells. We analyzed the influence of this effect on the generation of WT1-specific T cells and developed strategies for generating quantities of these cells from healthy donors, sufficient for adoptive T-cell therapies. HO-1 inhibition with SnMP increased WT1-specific T-cell frequencies in 13 (26%) of 50 healthy donors. To assess clinical applicability, we measured the enrichment efficiency of SnMP-treated WT1-specific T cells in response to a WT1-specific peptide pool and a HLA-A*02:01-restricted WT1 peptide by cytokine secretion assay. SnMP treatment resulted in a 28-fold higher enrichment efficacy with equal functionality. In conclusion, pharmacological inhibition of HO-1 activity with SnMP results in more efficient generation of functionally active WT1-specific T cells. This study demonstrates the therapeutic potentials of inhibiting HO-1 with SnMP to enhance antigen-specific T-cell responses in the treatment of cancer patients with WT1-positive disease.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Schillingmann DA,Riese SB,Vijayan V,Tischer-Zimmermann S,Schmetzer H,Maecker-Kolhoff B,Blasczyk R,Immenschuh S,Eiz-Vesper Bdoi
10.3390/ijms20030482subject
Has Abstractpub_date
2019-01-23 00:00:00issue
3issn
1422-0067pii
ijms20030482journal_volume
20pub_type
杂志文章abstract：:Chaetomium globosum is frequently isolated in water-damaged buildings and produces two mycotoxins called chaetoglobosins A and C when cultured on building material. In this study, the influence of ambient pH on the growth of C. globosum was examined on an artificial medium. This fungus was capable of growth on potato ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9122357
更新日期：2008-12-01 00:00:00
abstract：:Impaired NO-cGMP signaling has been linked to several neurological disorders. NO-sensitive guanylyl cyclase (NO-GC), of which two isoforms-NO-GC1 and NO-GC2-are known, represents a promising drug target to increase cGMP in the brain. Drug-like small molecules have been discovered that work synergistically with NO to s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082313
更新日期：2018-08-07 00:00:00
abstract：:Cadmium is a deleterious environmental pollutant that threats both animals and human health. Oxidative stress and elevated levels of reactive oxygen species (ROS) have recently been reported to be the main cause of cellular damage as a result of cadmium exposure. We investigate, here, the protective effect of strawber...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18050957
更新日期：2017-05-05 00:00:00
abstract：:The consumption of carotenoid-rich vegetables such as tomatoes and tomato sauces is associated with reduced risk of several chronic diseases. The predominant carotenoids in tomato products are in the (all-E) configuration, but (Z) isomers can be formed during thermal processing. The effect of cooking time (15, 30, 45 ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16059588
更新日期：2015-04-28 00:00:00
abstract：:A series of novel purpurinimides with long wavelength absorption were designed and synthesized to develop novel and potential photosensitizers. These compounds were investigated through reduction, oxidation, rearrangement reaction and amidation reactions of methyl pheophorbide a. They demonstrated a considerable batho...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15058091
更新日期：2014-05-08 00:00:00
abstract：:: Background: The aberrant expression of microRNAs (miRNAs) has been associated with several diseases, including cancer, inflammatory, and autoimmune conditions. Interest in salivary miRNAs as non-invasive tools for the diagnosis of malignancies and systemic diseases is rapidly increasing. The present systematic revie...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21030907
更新日期：2020-01-30 00:00:00
abstract：:Cardiovascular diseases (CVDs) are the leading causes of death worldwide. CVD pathophysiology is often characterized by increased stiffening of the heart muscle due to fibrosis, thus resulting in diminished cardiac function. Fibrosis can be caused by increased oxidative stress and inflammation, which is strongly linke...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21165913
更新日期：2020-08-18 00:00:00
abstract：:Recently, extreme ultraviolet (EUV) radiation has been increasingly used to modify polymers. Properties such as the extremely short absorption lengths in polymers and the very strong interaction of EUV photons with materials may play a key role in achieving new biomaterials. The purpose of the study was to examine the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249679
更新日期：2020-12-18 00:00:00
abstract：:Cardiac hypertrophy is an adaptive and compensatory mechanism preserving cardiac output during detrimental stimuli. Nevertheless, long-term stimuli incite chronic hypertrophy and may lead to heart failure. In this review, we analyze the recent literature regarding the role of ERK (extracellular signal-regulated kinase...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20092164
更新日期：2019-05-01 00:00:00
abstract：:Background: Gastric glands grow and cells reach differentiation at weaning in rats. By considering that early weaning (EW) can affect the timing of development, we aimed to compare molecular and cellular markers of differentiation in pups and adults. Methods: Wistar rats were separated into suckling-control (S) and EW...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010196
更新日期：2019-12-27 00:00:00
abstract：:G protein-coupled receptors (GPCRs) have a substantial role in tumorigenesis and are described as a "cancer driver". Aberrant expression or activation of GPCRs leads to the deregulation of downstream signaling pathways, thereby promoting cancer progression. In hepatocellular carcinoma (HCC), the Wnt signaling pathway ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19010313
更新日期：2018-01-21 00:00:00
abstract：:Many biological cellular processes occur at the micro- or millisecond time scale. With traditional all-atom molecular modeling techniques it is difficult to investigate the dynamics of long time scales or large systems, such as protein aggregation or activation. Coarse graining (CG) can be used to reduce the number of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms11062393
更新日期：2010-06-09 00:00:00
abstract：:Timely and reliable distinction of sepsis from non-infectious systemic inflammatory response syndrome (SIRS) supports adequate antimicrobial therapy and saves lives but is clinically challenging. Blood transcriptional profiling promises to deliver insights into the pathomechanisms of SIRS and sepsis and to accelerate ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20092290
更新日期：2019-05-09 00:00:00
abstract：:Many microbial ecology studies have demonstrated profound changes in community composition caused by environmental pollution, as well as adaptation processes allowing survival of microbes in polluted ecosystems. Soil microbial communities in polluted areas with a long-term history of contamination have been shown to m...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17060933
更新日期：2016-06-14 00:00:00
abstract：:Ethanol abuse is a common issue in individuals with sedentary lifestyles, unbalanced diets, and metabolic syndrome. Both ethanol abuse and metabolic syndrome have negative impacts on the central nervous system, with effects including cognitive impairment and brain oxidative status deterioration. The combined effects o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020807
更新日期：2021-01-15 00:00:00
abstract：:Patients harbouring mutations in genes encoding C-type natriuretic peptide (CNP; NPPC) or its receptor guanylyl cyclase B (GC-B, NPR2) suffer from severe growth phenotypes; loss-of-function mutations cause achondroplasia, whereas gain-of-function mutations cause skeletal overgrowth. Although most of the effects of CNP...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22031076
更新日期：2021-01-22 00:00:00
abstract：:VDACs (voltage-dependent anion-selective channels) are pore-forming proteins of the outer mitochondrial membrane, whose permeability is primarily due to VDACs' presence. In higher eukaryotes, three isoforms are raised during the evolution: they have the same exon-intron organization, and the proteins show the same cha...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197388
更新日期：2020-10-07 00:00:00
abstract：:The authors wish to make the following corrections to this paper [...]. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,已发布勘误
doi：10.3390/ijms21124560
更新日期：2020-06-26 00:00:00
abstract：BACKGROUND:The determinants that mediate the interactions between microRNAs and the gut microbiome impacting on obesity are scarcely understood. Thus, the aim of this study was to investigate possible interactions between circulating microRNAs and gut microbiota composition in obesity. METHOD:The sample comprised 78 s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249509
更新日期：2020-12-14 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Its highly notorious persistence in clinical settings is attributed to its ability to form antibiotic-resistant biofilms. Biofilm is an architecture built mostly by...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21228671
更新日期：2020-11-17 00:00:00
abstract：:The applications of ZnO nanoparticles in agriculture have largely contributed to crop growth regulation, quality enhancement, and induction of stress tolerance, while the underlying mechanisms remain elusive. Herein, the involvement of melatonin synthesis and metabolism in the process of nano-ZnO induced drought toler...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21030782
更新日期：2020-01-25 00:00:00
abstract：:Protein kinases of the CK1 family can be involved in numerous physiological and pathophysiological processes. Dysregulated expression and/or activity as well as mutation of CK1 isoforms have previously been linked to tumorigenesis. Among all neoplastic diseases, colon and rectal cancer (CRC) represent the fourth leadi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246184
更新日期：2019-12-07 00:00:00
abstract：:Multiple myeloma (MM) is a hematologic disorder of B lymphocytes characterized by the accumulation of malignant plasma cells (PCs) in the bone marrow. The altered plasma cells overproduce abnormal monoclonal immunoglobulins and also stimulate osteoclasts. The host's immune system and microenvironment are of paramount ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145002
更新日期：2020-07-15 00:00:00
abstract：:Genealogical concordance is a critical overlay of all phylogenetic analyses, irrespective of taxonomic level. To assess such patterns of congruence we have compiled and derived sequence data for two mitochondrial (16S rRNA, COI) and two nuclear (ITS1, p26) markers in 14 American populations of the hypersaline branchio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10125455
更新日期：2009-12-18 00:00:00
abstract：:Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor cells. In our experi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14010411
更新日期：2012-12-24 00:00:00
abstract：:Proteomic analyses are rapid and powerful tools that are used to increase the understanding of cancer pathogenesis, discover cancer biomarkers and predictive markers, and select and monitor novel targets for cancer therapy. Feline injection-site sarcomas (FISS) are aggressive skin tumours with high recurrence rates, d...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020576
更新日期：2018-02-14 00:00:00
abstract：:The development of stimulus-responsive photosensitizer delivery systems that carry a high payload of photosensitizers is of great importance in photodynamic therapy. In this study, redox-responsive polysilsesquioxane nanoparticles (PSilQNPs) built by a reverse microemulsion approach using 5,10,15,20-tetrakis(carboxyph...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17010056
更新日期：2015-12-31 00:00:00
abstract：:DNA topoisomerase II (TOP2) activity involves a normally transient double-strand break intermediate in which the enzyme is coupled to DNA via a 5'-phosphotyrosyl bond. However, etoposide and other topoisomerase drugs poison the enzyme by stabilising this enzyme-bridged break, resulting in the accumulation of TOP2-DNA ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19072056
更新日期：2018-07-14 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) typically migrates and metastasizes. Interleukin-6 (IL-6) is a multifunctional cytokine associated with disease status and cancer outcomes. The effect of IL-6 on human OSCC cells, however, is unknown. Here, we showed that IL-6 increased cell migration and Intercellular adhesion mole...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15010545
更新日期：2014-01-06 00:00:00
abstract：:Molecular imprinted polymers (MIP) are considered one of the most promising selective and novel separation methods for removal phenolic compound in wastewater treatment. MIP are crosslinked polymeric materials that exhibit high binding capacity and selectivity towards a target molecule (template), purposely present du...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms10010354
更新日期：2009-01-01 00:00:00
