Drosophila as a model for studying cystic fibrosis pathophysiology of the gastrointestinal system.

abstract：:Each person's genome sequence has thousands of missense variants. Practical interpretation of their functional significance must rely on computational inferences in the absence of exhaustive experimental measurements. Here we analyzed the efficacy of these inferences in 33 de novo missense mutations revealed by sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miosge LA,Field MA,Sontani Y,Cho V,Johnson S,Palkova A,Balakishnan B,Liang R,Zhang Y,Lyon S,Beutler B,Whittle B,Bertram EM,Enders A,Goodnow CC,Andrews TD

更新日期：2015-09-15 00:00:00

abstract：:The DNA templates containing immediate early (IE) genes of human cytomegalovirus (CMV) were transcribed in vitro by using a HeLa cell extract. When IE region 1, 2, and 3 were used, transcription was detected qualitatively only from IE region 1. Transcription was detected with DNA representing IE region 2 when the IE r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomsen DR,Stenberg RM,Goins WF,Stinski MF

更新日期：1984-02-01 00:00:00

abstract：:Fluorescence photobleaching recovery and immunofluorescence methods have been used to study the lateral mobility and topographical distribution of a major cell surface glycoprotein (CSP). Both endogenous CSP and fluorescent-labeled exogenous CSP bind to the cell surface in a fibrillar pattern and are immobile on the e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlessinger J,Barak LS,Hammes GG,Yamada KM,Pastan I,Webb WW,Elson EL

更新日期：1977-07-01 00:00:00

abstract：:Rotaviruses are the single most common cause of fatal and severe childhood diarrheal illness worldwide (>125 million cases annually). Rotavirus shares structural and functional features with many viruses, such as the presence of segmented double-stranded RNA genomes selectively and tightly packed with a conserved numb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Periz J,Celma C,Jing B,Pinkney JN,Roy P,Kapanidis AN

更新日期：2013-07-16 00:00:00

abstract：:Depression, the most prevalent mental illness, is underdiagnosed and undertreated, highlighting the need to extend the scope of current screening methods. Here, we use language from Facebook posts of consenting individuals to predict depression recorded in electronic medical records. We accessed the history of Faceboo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eichstaedt JC,Smith RJ,Merchant RM,Ungar LH,Crutchley P,Preoţiuc-Pietro D,Asch DA,Schwartz HA

更新日期：2018-10-30 00:00:00

abstract：:Estimates of 21st Century global-mean surface temperature increase have generally been based on scenarios that do not include climate policies. Newly developed multigas mitigation scenarios, based on a wide range of modeling approaches and socioeconomic assumptions, now allow the assessment of possible impacts of clim...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Vuuren DP,Meinshausen M,Plattner GK,Joos F,Strassmann KM,Smith SJ,Wigley TM,Raper SC,Riahi K,de la Chesnaye F,den Elzen MG,Fujino J,Jiang K,Nakicenovic N,Paltsev S,Reilly JM

更新日期：2008-10-07 00:00:00

abstract：:As global climate continues to warm, melting of glaciers releases a large quantity of mercury (Hg) originally locked in ice into the atmosphere and downstream ecosystems. Here, we show an opposite process that captures atmospheric Hg through glacier-to-vegetation succession. Our study using stable isotope techniques a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Luo J,Yuan W,Lin CJ,Wang F,Liu C,Wang G,Feng X

更新日期：2020-01-28 00:00:00

abstract：:We have isolated and determined the sequence of a genomic clone containing the gene for a human class I transplantation antigen. The gene contains seven exons. The first five exons code respectively for a signal peptide, for the first, second, and third extracellular domains of the protein molecule, and for the transm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malissen M,Malissen B,Jordan BR

更新日期：1982-02-01 00:00:00

abstract：:The 158,000 Mr protein, previously designated C5c, present in fully assembled complement (C) membrane attack complexes (MC5b-9) has been identified as a disulfide-bonded dimer of C9. This conclusion was based on the observations that: (i) a portion of the 125I-radiolabeled precursor C9 incorporated into MC5b-9 complex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ware CF,Kolb WP

更新日期：1981-10-01 00:00:00

abstract：:A nuclear protein, termed leucine-rich acidic nuclear protein (LANP), has been isolated from among rat cerebellar proteins whose expression was transiently increased during an early stage of postnatal development. The amino acid sequence, deduced from its cDNA, showed that LANP contains 247 amino acids consisting of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuoka K,Taoka M,Satozawa N,Nakayama H,Ichimura T,Takahashi N,Yamakuni T,Song SY,Isobe T

更新日期：1994-10-11 00:00:00

abstract：:Lod scores provide a method to unify linkage tests based on identity by descent and identity in marker state while permitting selection of the most informative individuals through their disease-related phenotypes and markers in relatives. After parametric lods are reviewed, a nonparametric approach that depends on a s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morton NE

更新日期：1996-04-16 00:00:00

abstract：:Fibrotic diseases are not well-understood. They represent a number of different diseases that are characterized by the development of severe organ fibrosis without any obvious cause, such as the devastating diseases idiopathic pulmonary fibrosis (IPF) and scleroderma. These diseases have a poor prognosis comparable wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wernig G,Chen SY,Cui L,Van Neste C,Tsai JM,Kambham N,Vogel H,Natkunam Y,Gilliland DG,Nolan G,Weissman IL

更新日期：2017-05-02 00:00:00

abstract：:We have determined the nucleotide sequences of the Klebsiella pneumoniae nifL (regulation of N2 fixation genes) and the Escherichia coli glnA (glutamine synthetase) promoters. We compared these sequences with the published sequences of three other promoters that, like the nifL and glnA promoters, are activated by the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ow DW,Sundaresan V,Rothstein DM,Brown SE,Ausubel FM

更新日期：1983-05-01 00:00:00

abstract：:The fluorescence decay functions of individual, specifically labeled tRNAPhe molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNAPhe conformational states that interchange much more slowly are evidenced by the distribution of life...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jia Y,Sytnik A,Li L,Vladimirov S,Cooperman BS,Hochstrasser RM

更新日期：1997-07-22 00:00:00

abstract：:Aconitase (Aco1p) is a multifunctional protein: It is an enzyme of the tricarboxylic acid cycle. In animal cells, Aco1p also is a cytosolic protein binding to mRNAs to regulate iron metabolism. In yeast, Aco1p was identified as a component of mtDNA nucleoids. Here we show that yeast Aco1p protects mtDNA from excessive...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen XJ,Wang X,Butow RA

更新日期：2007-08-21 00:00:00

abstract：:In an earlier paper, the author jointly with S. Suryawanshi proposed statistical analysis of shape through triangulation of landmarks on objects. It was observed that the angles of the triangles are invariant to scaling, location, and rotation of objects. No distinction was made between an object and its reflection. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rao CR

更新日期：2000-03-28 00:00:00

abstract：:An ability to safely harness the powerful regenerative potential of adult stem cells for clinical applications is critically dependent on a comprehensive understanding of the underlying mechanisms regulating their activity. Epithelial organoid cultures accurately recapitulate many features of in vivo stem cell-driven ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami K,Terakado Y,Saito K,Jomen Y,Takeda H,Oshima M,Barker N

更新日期：2021-01-26 00:00:00

abstract：:Conventional methods of determining the coupling factor alpha(2)(omega)F(omega) for the newly discovered high transition temperature (T(c)) cuprate superconductors by using tunneling and infrared measurements have thus far failed to show the cause of the very high T(c) of these compounds. This is due in part to diffic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Little WA,Collman JP

更新日期：1988-07-01 00:00:00

abstract：:Human speech comprehension is remarkable for its immediacy and rapidity. The listener interprets an incrementally delivered auditory input, millisecond by millisecond as it is heard, in terms of complex multilevel representations of relevant linguistic and nonlinguistic knowledge. Central to this process are the neura...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyu B,Choi HS,Marslen-Wilson WD,Clarke A,Randall B,Tyler LK

更新日期：2019-10-15 00:00:00

abstract：:Genetic screens have been performed to identify mutants with altered auxin homeostasis in Arabidopsis. A tagged allele of the auxin-overproducing mutant sur2 was identified within a transposon mutagenized population. The SUR2 gene was cloned and shown to encode the CYP83B1 protein, which belongs to the large family of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barlier I,Kowalczyk M,Marchant A,Ljung K,Bhalerao R,Bennett M,Sandberg G,Bellini C

更新日期：2000-12-19 00:00:00

abstract：:Arachidonic acid (AA) metabolites derived from both cyclooxygenase (COX) and lipoxygenase (LOX) pathways transduce a variety of signals related to cell growth. Here, we report that the AA LOX pathway also functions as a critical regulator of cell survival and apoptosis. Rat Walker 256 (W256) carcinosarcoma cells expre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tang DG,Chen YQ,Honn KV

更新日期：1996-05-28 00:00:00

abstract：:Nipah virus (NiV) and Hendra virus are the type species of the highly pathogenic paramyxovirus genus Henipavirus, which can cause severe respiratory disease and fatal encephalitis infections in humans, with case fatality rates approaching 75%. NiV contains two envelope glycoproteins, the receptor-binding G glycoprotei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu K,Rajashankar KR,Chan YP,Himanen JP,Broder CC,Nikolov DB

更新日期：2008-07-22 00:00:00

abstract：:Understanding the gene-expression patterns during liver regeneration may help to reveal how regenerative processes are initiated and controlled as well as shed new light onto processes that lead to liver disease. Using high-density oligonucleotide arrays, we have examined the gene-expression program in the livers of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Su AI,Guidotti LG,Pezacki JP,Chisari FV,Schultz PG

更新日期：2002-08-20 00:00:00

abstract：:Purified dicyclohexylcarbodiimide-sensitive ATPase (TF0-F1) from thermophilic bacterium PS3 is composed of a water soluble part with ATP hydrolytic activity (TF1) and a water insoluble moiety (TF0). All of the five subunits (alpha, beta, gamma, delta, and epsilon) of TF1 were isolated. TF1 was reconstituted from the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshida M,Okamoto H,Sone N,Hirata H,Kagawa Y

更新日期：1977-03-01 00:00:00

abstract：:Mesenchymal stromal stem cells (MSCs) isolated from adult tissues offer tangible potential for regenerative medicine, given their feasibility for autologous transplantation. MSC research shows encouraging results in experimental stroke, amyotrophic lateral sclerosis, and neurotrauma models. However, further translatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ropper AE,Thakor DK,Han I,Yu D,Zeng X,Anderson JE,Aljuboori Z,Kim SW,Wang H,Sidman RL,Zafonte RD,Teng YD

更新日期：2017-01-31 00:00:00

abstract：:The potential involvement of ceramide-related signaling processes in the induction of apoptosis by tumor necrosis factor alpha was assessed by multiple biochemical strategies in the human leukemic cell lines HL-60 and U937 and the murine fibrosarcoma cell lines L929/LM and WEHI 164/13. Exposure of these cells to tumor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jarvis WD,Kolesnick RN,Fornari FA,Traylor RS,Gewirtz DA,Grant S

更新日期：1994-01-04 00:00:00

abstract：:Immunological memory is a hallmark of adaptive immunity, and understanding T cell memory will be central to the development of effective cell-mediated vaccines. The characteristics and functions of CD4 memory cells have not been well defined. Here we demonstrate that the increased size of the secondary response is sol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MacLeod MK,McKee A,Crawford F,White J,Kappler J,Marrack P

更新日期：2008-09-23 00:00:00

abstract：:During excitation-contraction (e-c) coupling of striated muscle, depolarization of the surface membrane is converted into Ca2+ release from internal stores. This process occurs at intracellular junctions characterized by a specialized composition and structural organization of membrane proteins. The coordinated arrang...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flucher BE,Franzini-Armstrong C

更新日期：1996-07-23 00:00:00

abstract：:Examination of old human lenses indicates that low-molecular-weight alpha crystallin is not present in the inner 30-40%, the nucleus, of the lens. The remainder of such lenses, the periphery, contains normal levels of this protein. This finding is in marked contrast to observations in young lenses, where a large quant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy D,Spector A

更新日期：1976-10-01 00:00:00

abstract：:We examined the effect of the inhibition of initiation of DNA replication on the membrane association of the chromosomal origin of replication of Bacillus subtilis and the Staphylococcus aureus-Bacillus pumilus chimeric plasmid pSL103, using temperature-sensitive mutants of B. subtilis that have specifically affected ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winston S,Sueoka N

更新日期：1980-05-01 00:00:00