Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA.

abstract：:The lactose permease of Escherichia coli catalyzes coupled translocation of galactosides and H(+) across the cell membrane. It is the best-characterized member of the Major Facilitator Superfamily, a related group of membrane proteins with 12 transmembrane domains that mediate transport of various substrates across ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sorgen PL,Hu Y,Guan L,Kaback HR,Girvin ME

更新日期：2002-10-29 00:00:00

abstract：:Clustered DNA damage induced by ionizing radiation is refractory to repair and may trigger carcinogenic events for reasons that are not well understood. Here, we used an in situ method to directly monitor induction and repair of clustered DNA lesions in individual cells. We showed, consistent with biophysical modeling...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asaithamby A,Hu B,Chen DJ

更新日期：2011-05-17 00:00:00

abstract：:We have produced a soluble form of a mouse alpha beta T-cell antigen receptor (TCR) by shuffling its variable (V) and constant (C) domains to the C region of an immunoglobulin kappa light chain. These chimeric molecules composed of V alpha C alpha C kappa and V beta C beta C kappa chains were efficiently secreted (up ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grégoire C,Rebaï N,Schweisguth F,Necker A,Mazza G,Auphan N,Millward A,Schmitt-Verhulst AM,Malissen B

更新日期：1991-09-15 00:00:00

abstract：:Continuous intravenous infusion of heparin suppresses smooth muscle cell proliferation in rats after endothelial injury but may lead to hemorrhage and other complications. The anticoagulant property has been removed from chemically modified heparin without loss of antiproliferative effect but use of such compounds is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edelman ER,Adams DH,Karnovsky MJ

更新日期：1990-05-01 00:00:00

abstract：:Double-stranded DNA break repair by homologous recombination is initiated by resection of free DNA ends to produce a 3'-ssDNA overhang. In bacteria, this reaction is catalyzed by helicase-nuclease complexes such as AddAB in a manner regulated by specific recombination hotspot sequences called Crossover hotspot instiga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrasco C,Gilhooly NS,Dillingham MS,Moreno-Herrero F

更新日期：2013-07-09 00:00:00

abstract：:Animals capable of regenerating multiple tissue types, organs, and appendages after injury are common yet sporadic and include some sponge, hydra, planarian, and salamander (i.e., newt and axolotl) species, but notably such regenerative capacity is rare in mammals. The adult MRL mouse strain is a rare exception to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bedelbaeva K,Snyder A,Gourevitch D,Clark L,Zhang XM,Leferovich J,Cheverud JM,Lieberman P,Heber-Katz E

更新日期：2010-03-30 00:00:00

abstract：:The effects of calcium ion on the Na+ activation gate were studied in squid giant axons. Saxitoxin (STX) was used to block ion entry into Na+ channels without hindering access to the membrane surface, making it possible to distinguish surface effects of calcium from pore-occupancy effects. In the presence of STX, gati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armstrong CM

更新日期：1999-03-30 00:00:00

abstract：:Antibodies may bind to bacterial pathogens or their toxins to control infections, and their effector activity is mediated through the recruitment of complement component C1q or the engagement with Fcγ receptors (FcγRs). For bacterial pathogens that rely on a single toxin to cause disease, immunity correlates with toxi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Shi M,Tong X,Kim HK,Wang LX,Schneewind O,Missiakas D

更新日期：2020-09-15 00:00:00

abstract：:Point mutations in vascular smooth muscle α-actin (SM α-actin), encoded by the gene ACTA2, are the most prevalent cause of familial thoracic aortic aneurysms and dissections (TAAD). Here, we provide the first molecular characterization, to our knowledge, of the effect of the R258C mutation in SM α-actin, expressed wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu H,Fagnant PM,Bookwalter CS,Joel P,Trybus KM

更新日期：2015-08-04 00:00:00

abstract：:Extracellular growth and differentiation factors induce changes in gene expression in the nucleus by initiating a series of protein associations that alter the subcellular localization of intracellular signaling proteins. Initial events involve receptor homo- or heterodimerization and subsequent recruitment of cytosol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belshaw PJ,Ho SN,Crabtree GR,Schreiber SL

更新日期：1996-05-14 00:00:00

abstract：:Introduction and expression of multiple transgenes is frequently required for basic and applied studies. However, at present, multigene transformation is very difficult due to technical limitations of existing methods. Here, we describe a vector system for efficient multigene assembly and transformation. The system co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin L,Liu YG,Xu X,Li B

更新日期：2003-05-13 00:00:00

abstract：:Alfalfa mosaic virus (AlMV) coat protein is involved in systemic infection of host plants, and a specific mutation in this gene prevents the virus from moving into the upper uninoculated leaves. The coat protein also is required for different viral functions during early and late infection. To study the role of the co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spitsin S,Steplewski K,Fleysh N,Belanger H,Mikheeva T,Shivprasad S,Dawson W,Koprowski H,Yusibov V

更新日期：1999-03-02 00:00:00

abstract：:Two Chinese hamster cell lines, G1+-1 and CHO, have been grown in the presence of low concentrations of hydroxyurea to determine how a slowing DNA synthesis (i.e., a lengthening of the S period) affects the length of the G1 period. Hydroxyurea concentrations of approximately 10 microM do not alter the generation times...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stancel GM,Prescott DM,Liskay RM

更新日期：1981-10-01 00:00:00

abstract：:Strategies to elicit Abs that can neutralize diverse strains of a highly mutable pathogen are likely to result in a potent vaccine. Broadly neutralizing Abs (bnAbs) against HIV have been isolated from patients, proving that the human immune system can evolve them. Using computer simulations and theory, we study immuni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaffer JS,Moore PL,Kardar M,Chakraborty AK

更新日期：2016-11-08 00:00:00

abstract：:The Ten-Eleven-Translocation 2 (TET2) gene encodes a member of TET family enzymes that alters the epigenetic status of DNA by oxidizing 5-methylcytosine to 5-hydroxymethylcytosine (5hmC). Somatic loss-of-function mutations of TET2 are frequently observed in patients with diverse myeloid malignancies, including myelody...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ko M,Bandukwala HS,An J,Lamperti ED,Thompson EC,Hastie R,Tsangaratou A,Rajewsky K,Koralov SB,Rao A

更新日期：2011-08-30 00:00:00

abstract：:The acquisition of cell-surface urokinase plasminogen activator activity is a hallmark of malignancy. We generated an engineered anthrax toxin that is activated by cell-surface urokinase in vivo and displays limited toxicity to normal tissue but broad and potent tumoricidal activity. Native anthrax toxin protective an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu S,Aaronson H,Mitola DJ,Leppla SH,Bugge TH

更新日期：2003-01-21 00:00:00

abstract：:Recently, we established that satellite III (TGGAA)n tandem repeats, which occur at the centromeres of human chromosomes, pair with themselves to form an unusual "self-complementary" antiparallel duplex containing (GGA)2 motifs in which two unpaired guanines from opposite strands intercalate between sheared G.A base p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Chou SH,Reid BR

更新日期：1996-10-29 00:00:00

abstract：:An RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) has been purified from phage-SP01-infected Bacillus subtilis that copies RNA almost exclusively from the heavy strand of native SP01 DNA, the DNA strand from which "middle" and "late" classes of RNA are copied in vivo. Hybridization-com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pero J,Nelson J,Fox TD

更新日期：1975-04-01 00:00:00

abstract：:Infectious RNA transcripts were generated from full-length cDNA clones of the tobacco etch potyvirus genome containing an insertion of the bacterial beta-glucuronidase (GUS) gene between the polyprotein-coding sequences for the N-terminal 35-kDa proteinase and the helper component-proteinase. The recombinant virus was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dolja VV,McBride HJ,Carrington JC

更新日期：1992-11-01 00:00:00

abstract：:Genetic data are commonly used to estimate connectivity between putative populations, but translating them to demographic dispersal rates is complicated. Theoretical equations that infer a migration rate based on the genetic estimator F ST , such as Wright's equation, F ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spies I,Hauser L,Jorde PE,Knutsen H,Punt AE,Rogers LA,Stenseth NC

更新日期：2018-05-08 00:00:00

abstract：:Cell recognition molecules play a crucial role in the regulation of immune cells. We recently found that mast cells (MCs) express leukocyte recognition molecules, including ICAM-1 antigen, a natural ligand of LFA-1. We here report that interleukin 4 (IL-4), a pleiotropic cytokine and mast cell differentiation factor, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valent P,Bevec D,Maurer D,Besemer J,Di Padova F,Butterfield JH,Speiser W,Majdic O,Lechner K,Bettelheim P

更新日期：1991-04-15 00:00:00

abstract：:The self-association of gene-32 protein has been studied by sedimentation equilibrium centrifugation and polyacrylamide gel electrophoresis, in order to better understand its role in DNA replication and genetic recombination. The monomer molecular weight of gene-32 protein is 38,000 in guanidine hydrochloride and 34,0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carroll RB,Neet KE,Goldthwait DA

更新日期：1972-09-01 00:00:00

abstract：:The size and metabolic rate of cells affect processes from the molecular to the organismal level. We present a quantitative, theoretical framework for studying relationships among cell volume, cellular metabolic rate, body size, and whole-organism metabolic rate that helps reveal the feedback between these levels of o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Savage VM,Allen AP,Brown JH,Gillooly JF,Herman AB,Woodruff WH,West GB

更新日期：2007-03-13 00:00:00

abstract：:The remarkable fidelity of most DNA polymerases depends on a series of early steps in the reaction pathway which allow the selection of the correct nucleotide substrate, while excluding all incorrect ones, before the enzyme is committed to the chemical step of nucleotide incorporation. The conformational transitions t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santoso Y,Joyce CM,Potapova O,Le Reste L,Hohlbein J,Torella JP,Grindley ND,Kapanidis AN

更新日期：2010-01-12 00:00:00

abstract：:For several decades, research into the mechanisms of genetic recombination proceeded without a complete understanding of its cellular function or its place in DNA metabolism. Many lines of research recently have coalesced to reveal a thorough integration of most aspects of DNA metabolism, including recombination. In b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cox MM

更新日期：2001-07-17 00:00:00

abstract：:The rapidly expanding network of roads into the Amazon is permanently altering the world's largest tropical forest. Most proposed road projects lack rigorous impact assessments or even basic economic justification. This study analyzes the expected environmental, social and economic impacts of 75 road projects, totalin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vilela T,Malky Harb A,Bruner A,Laísa da Silva Arruda V,Ribeiro V,Auxiliadora Costa Alencar A,Julissa Escobedo Grandez A,Rojas A,Laina A,Botero R

更新日期：2020-03-31 00:00:00

abstract：:Transporters in the Golgi apparatus membrane translocate nucleotide sugars from the cytosol into the Golgi lumen before these can be substrates for the glycosylation of proteins, lipids, and proteoglycans. We have cloned the mammalian Golgi membrane transporter for uridine diphosphate-N-acetylglucosamine by phenotypic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guillen E,Abeijon C,Hirschberg CB

更新日期：1998-07-07 00:00:00

abstract：:Activated RAS transforming genes that encode proteins (p21s) with amino acid substitutions at positions 12, 13, or 61 have been detected in 10-20% of human neoplasms. This report describes a monoclonal antibody (DWP) raised against a synthetic peptide corresponding to amino acids 5-16 of a mutated RAS gene encoding Va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carney WP,Petit D,Hamer P,Der CJ,Finkel T,Cooper GM,Lefebvre M,Mobtaker H,Delellis R,Tischler AS

更新日期：1986-10-01 00:00:00

abstract：:Group A Streptococcus (GAS) and other bacterial pathogens are known to interact with integrins as an initial step in a complex pathway of bacterial ingestion by host cells. Efficient GAS invasion depends on the interaction of bound fibronectin (Fn) with integrins and activation of integrin signaling. TGF-beta1 regulat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang B,Li S,Southern PJ,Cleary PP

更新日期：2006-02-14 00:00:00

abstract：:Simplified models of the protein-folding process have led to valuable insights into the generic properties of the folding of heteropolymers. On the basis of theoretical arguments, Shakhnovich and Gutin [(1993) Proc. Natl. Acad. Sci. USA 90, 7195-7199] have proposed a specific method to generate folding sequences for o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ebeling M,Nadler W

更新日期：1995-09-12 00:00:00