Most of the G1 period in hamster cells is eliminated by lengthening the S period.

Abstract:

:Two Chinese hamster cell lines, G1+-1 and CHO, have been grown in the presence of low concentrations of hydroxyurea to determine how a slowing DNA synthesis (i.e., a lengthening of the S period) affects the length of the G1 period. Hydroxyurea concentrations of approximately 10 microM do not alter the generation times of these cell lines but do cause increases in S with corresponding decreases in G1. In both cell lines, 10 microM hydroxyurea reduces G1 to an absolute value of 1 hr, which represents decreases of 70% (G1+-1) and 60% (CHO) from control values. Higher concentrations of hydroxyurea increase the generation times and lengths of S for both cell lines but do not reduce G1 below the minimum value of 1 hr. These observations indicate that the majority of G1 is expendable and most of G1 therefore cannot contain specific events required for the initiation of DNA synthesis. This result supports the hypothesis that G1 is a portion of the cell growth cycle but not of the chromosome cycle.

authors

Stancel GM,Prescott DM,Liskay RM

doi

10.1073/pnas.78.10.6295

subject

Has Abstract

pub_date

1981-10-01 00:00:00

pages

6295-8

issue

10

eissn

0027-8424

issn

1091-6490

journal_volume

78

pub_type

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