Abstract:
:Rotaviruses are the single most common cause of fatal and severe childhood diarrheal illness worldwide (>125 million cases annually). Rotavirus shares structural and functional features with many viruses, such as the presence of segmented double-stranded RNA genomes selectively and tightly packed with a conserved number of transcription complexes in icosahedral capsids. Nascent transcripts exit the capsid through 12 channels, but it is unknown whether these channels specialize in specific transcripts or simply act as general exit conduits; a detailed description of this process is needed for understanding viral replication and genomic organization. To this end, we developed a single molecule assay for capturing and identifying transcripts extruded from transcriptionally active viral particles. Our findings support a model in which each channel specializes in extruding transcripts of a specific segment that in turn is linked to a single transcription complex. Our approach can be extended to study other viruses and transcription systems.
journal_name
Proc Natl Acad Sci U S Aauthors
Periz J,Celma C,Jing B,Pinkney JN,Roy P,Kapanidis ANdoi
10.1073/pnas.1220345110subject
Has Abstractpub_date
2013-07-16 00:00:00pages
12042-7issue
29eissn
0027-8424issn
1091-6490pii
1220345110journal_volume
110pub_type
杂志文章abstract::The physiological significance of multiple G-protein-coupled receptor subtypes, such as the beta-adrenergic receptors (beta ARs), remains obscure, since in many cases several subtypes activate the same effector and utilize the same physiological agonists. We inspected the deduced amino acid sequences of the beta AR su...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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