Nonexponential kinetics of a single tRNAPhe molecule under physiological conditions.

abstract：:Myosin VI is a molecular motor implicated in many processes, and it likely associates with a variety of cargoes that specify its functions. Although it is critical to Drosophila development, little is known about its cellular roles. To reveal its involvement in specific pathways, we sought to identify the binding part...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finan D,Hartman MA,Spudich JA

更新日期：2011-04-05 00:00:00

abstract：:The development of osteoclastic cell lines would greatly facilitate analysis of the cellular and molecular biology of bone resorption. Several cell lines have previously been reported to be capable of osteoclastic differentiation. However, such cell lines form at best only occasional excavations, suggesting that osteo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chambers TJ,Owens JM,Hattersley G,Jat PS,Noble MD

更新日期：1993-06-15 00:00:00

abstract：:Polyclonal antibodies reactive with a cleavage site in human proinsulin (HPI) (C-peptide-A-chain junction) have been raised (rabbit, guinea pig) using a synthetic peptide antigen coupled with keyhole limpet hemocyanin. These antisera recognize native HPI and des-31,32-HPI equally well but react 20-50 times less well w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steiner DF,Michael J,Houghten R,Mathieu M,Gardner PR,Ravazzola M,Orci L

更新日期：1987-09-01 00:00:00

abstract：:B-1a cells reside in both the peritoneal cavity and the spleen. LPS stimulates splenic B-1a to differentiate to plasma cells producing natural IgM specific for microbial and self antigens. However, there are conflicting views as to whether the B-1a cells divide before this differentiation occurs, and hence how the res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Y,Tung JW,Ghosn EE,Herzenberg LA,Herzenberg LA

更新日期：2007-03-13 00:00:00

abstract：:X-linked hypophosphatemia is the most prevalent inherited form of rickets. In this disorder, rickets results from hyperphosphaturia and inappropriately normal levels of 1,25(OH)2-vitamin D. Current therapy with oral phosphate and vitamin D improves the rickets, but has significant morbidity and does not significantly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baum M,Loleh S,Saini N,Seikaly M,Dwarakanath V,Quigley R

更新日期：2003-09-16 00:00:00

abstract：:The formation of nerve-muscle junctions in monolayer cultures of embryonic muscle and spinal cord cells is described. Muscle-forming cells (myoblasts) from leg muscles of 12-day chick embryos were separated with trypsin and cultured on a collagen substrate for two days. Suspensions of ventral spinal cord cells from si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shimada Y,Fischman DA,Moscona AA

更新日期：1969-03-01 00:00:00

abstract：:A central tenet of evolutionary social science holds that behaviors, such as those associated with social dominance, produce fitness effects that are subject to cultural selection. However, evidence for such selection is inconclusive because it is based on short-term statistical associations between behavior and ferti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lansing JS,Watkins JC,Hallmark B,Cox MP,Karafet TM,Sudoyo H,Hammer MF

更新日期：2008-08-19 00:00:00

abstract：:Expressions are developed for weak single pair emission probability and strong emission average number of pairs. The water transparency cutoff is closely realized, showing that the fundamental time scale is even shorter. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwinger J

更新日期：1993-05-15 00:00:00

abstract：:Previous studies have shown that DNA methyltransferase (Dnmt) 1 is required for maintenance of bulk DNA methylation and is essential for mouse development. However, somatic disruption of DNMT1 in the human cancer cell line HCT116 was not lethal and caused only minor decreases in methylation. Here, we report the identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egger G,Jeong S,Escobar SG,Cortez CC,Li TW,Saito Y,Yoo CB,Jones PA,Liang G

更新日期：2006-09-19 00:00:00

abstract：:This paper's findings suggest that an arbitrary Chinese policy that greatly increases total suspended particulates (TSPs) air pollution is causing the 500 million residents of Northern China to lose more than 2.5 billion life years of life expectancy. The quasi-experimental empirical approach is based on China's Huai ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Ebenstein A,Greenstone M,Li H

更新日期：2013-08-06 00:00:00

abstract：:Differentiation-inducing factor 1 [DIF-1; 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)-hexan-1-one] induces stalk cell differentiation during Dictyostelium development. It is present as a gradient in the multicellular slug, its lowest concentration being in the anterior. Here we demonstrate the existence of a locali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kay RR,Large S,Traynor D,Nayler O

更新日期：1993-01-15 00:00:00

abstract：:The Arabidopsis DEMETER (DME) DNA glycosylase demethylates the maternal genome in the central cell prior to fertilization and is essential for seed viability. DME preferentially targets small transposons that flank coding genes, influencing their expression and initiating plant gene imprinting. DME also targets interg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang C,Hung YH,Rim HJ,Zhang D,Frost JM,Shin H,Jang H,Liu F,Xiao W,Iyer LM,Aravind L,Zhang XQ,Fischer RL,Huh JH,Hsieh TF

更新日期：2019-08-27 00:00:00

abstract：:Human embryonic stem cells have the potential to differentiate into various cell types and, thus, may be useful as a source of cells for transplantation or tissue engineering. We describe here the differentiation steps of human embryonic stem cells into endothelial cells forming vascular-like structures. The human emb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levenberg S,Golub JS,Amit M,Itskovitz-Eldor J,Langer R

更新日期：2002-04-02 00:00:00

abstract：:The electronic absorption spectra of oxidized and reduced spinach ferredoxins have been measured between 1200 and 600 nm at low temperature in D(2)O/ethylene glycol glasses. Relatively weak absorption bands are observed at 720, 820, and 920 nm in oxidized ferredoxin, and at 652, 820, and 920 nm in reduced ferredoxin. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rawlings J,Siiman O,Gray HB

更新日期：1974-01-01 00:00:00

abstract：:The issue of protein dynamics and its implications in the biological function of proteins are arousing greater and greater interest in different fields of molecular biology. In cryo-electron tomography experiments one may take several snapshots of a given biological macromolecule. In principle, a large enough collecti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bongini L,Fanelli D,Piazza F,De Los Rios P,Sandin S,Skoglund U

更新日期：2004-04-27 00:00:00

abstract：:Niemann-Pick type C (NPC) 1 protein plays important roles in moving cholesterol and other lipids out of late endosomes by means of vesicular trafficking, but it is not known whether NPC1 directly interacts with cholesterol. We performed photoaffinity labeling of intact cells expressing fluorescent protein (FP)-tagged ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohgami N,Ko DC,Thomas M,Scott MP,Chang CC,Chang TY

更新日期：2004-08-24 00:00:00

abstract：:Seed development in plants involves the coordinated growth of the embryo, endosperm, and maternal tissue. Several genes have been identified that influence seed size by acting maternally, such as AUXIN RESPONSE FACTOR2, APETALA2, and DA1. However, given the lack of gain-of-function effects of these genes on seed size,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adamski NM,Anastasiou E,Eriksson S,O'Neill CM,Lenhard M

更新日期：2009-11-24 00:00:00

abstract：:Most genetic regulatory mechanisms involve protein-DNA interactions. In these processes, the classical Watson-Crick DNA structure sometimes is distorted severely, which in turn enables the precise recognition of the specific sites by the protein. Despite its key importance, very little is known about such deformation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leger JF,Robert J,Bourdieu L,Chatenay D,Marko JF

更新日期：1998-10-13 00:00:00

abstract：:The mammalian form of the protozoan parasite Leishmania mexicana contains high activity of a cysteine proteinase (LmCPb) encoded on a tandem array of 19 genes (lmcpb). Homozygous null mutants for lmcpb have been produced by targeted gene disruption. All life-cycle stages of the mutant can be cultured in vitro, demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mottram JC,Souza AE,Hutchison JE,Carter R,Frame MJ,Coombs GH

更新日期：1996-06-11 00:00:00

abstract：:The reversible phosphorylation of tyrosine residues is an important mechanism for modulating biological processes such as cellular signaling, differentiation, and growth, and if deregulated, can result in various types of cancer. Therefore, an understanding of these dynamic cellular processes at the molecular level re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salomon AR,Ficarro SB,Brill LM,Brinker A,Phung QT,Ericson C,Sauer K,Brock A,Horn DM,Schultz PG,Peters EC

更新日期：2003-01-21 00:00:00

abstract：:Orai1 and stromal interaction molecule 1 (STIM1) mediate store-operated Ca(2+) entry (SOCE) in immune cells. STIM1, an endoplasmic reticulum (ER) Ca(2+) sensor, detects store depletion and interacts with plasma membrane (PM)-resident Orai1 channels at the ER-PM junctions. However, the molecular composition of these ju...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woo JS,Srikanth S,Nishi M,Ping P,Takeshima H,Gwack Y

更新日期：2016-03-08 00:00:00

abstract：:I describe the self-splicing of an RNA that consists of exon sequences flanked by group II intron sequences. I find that this RNA undergoes accurate splicing in vitro, yielding an excised exon circle. This splicing reaction involves the joining of the 5' splice site at the end of an exon to the 3' splice site at the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jarrell KA

更新日期：1993-09-15 00:00:00

abstract：:Engagement of high-affinity IgE receptors leads to activation of tyrosine and serine/threonine kinases and the immediate phosphorylation of receptor beta (serine and tyrosine) and gamma (threonine and tyrosine) chains. Receptor disengagement leads to dephosphorylation of beta and gamma chains via the action of undefin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paolini R,Numerof R,Kinet JP

更新日期：1992-11-15 00:00:00

abstract：:Deletion derivatives of the maize transposable element Activator (Ac) were constructed in vitro and inserted into a kanamycin resistance gene. These constructions were then introduced into tobacco protoplasts derived from plants previously transformed with Ac. The ability of each deletion derivative to excise was meas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coupland G,Plum C,Chatterjee S,Post A,Starlinger P

更新日期：1989-12-01 00:00:00

abstract：:After intravitreal injection of gamma-[3H] aminobutyric acid (GAB), 2% of the neurons at the outer margin of the inner plexiform layer were intensely labeled. Reconstructions of these neurons from serial electron microscope autoradiograms showed that they are interplexiform cells, which synapse on bipolar processes in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,McGuire BA,Sterling P

更新日期：1980-01-01 00:00:00

abstract：:Recombinant techniques allow one to engineer an antibody molecule and, in this way, manipulate its properties and functions. We engineered a chimeric human/mouse antibody to the tumor-associated antigen ganglioside GD2, with the aim of decreasing its serum half-life, maintaining its full antigen-binding capacity, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mueller BM,Reisfeld RA,Gillies SD

更新日期：1990-08-01 00:00:00

abstract：:Hearing loss is the main limitation of radiation therapy for vestibular schwannoma (VS), and identifying treatment options that minimize hearing loss are urgently needed. Treatment with bevacizumab is associated with tumor control and hearing improvement in neurofibromatosis type 2 (NF2) patients; however, its effect ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao X,Zhao Y,Stemmer-Rachamimov AO,Liu H,Huang P,Chin S,Selig MK,Plotkin SR,Jain RK,Xu L

更新日期：2015-11-24 00:00:00

abstract：:Overexpression of the p53 protein, resulting from gene mutations that increase protein stability, has been detected in greater than 25% of primary human breast cancers. In addition, approximately 10% of breast cancer patients have circulating antibodies to the p53 protein. In this study, the anti-p53 humoral response ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davidoff AM,Iglehart JD,Marks JR

更新日期：1992-04-15 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a lethal genetic disorder caused by an absence of the dystrophin protein in bodywide muscles, including the heart. Cardiomyopathy is a leading cause of death in DMD. Exon skipping via synthetic phosphorodiamidate morpholino oligomers (PMOs) represents one of the most promising ther...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Echigoya Y,Nakamura A,Nagata T,Urasawa N,Lim KRQ,Trieu N,Panesar D,Kuraoka M,Moulton HM,Saito T,Aoki Y,Iversen P,Sazani P,Kole R,Maruyama R,Partridge T,Takeda S,Yokota T

更新日期：2017-04-18 00:00:00

abstract：:Freezing avoidance conferred by different types of antifreeze proteins in various polar and subpolar fishes represents a remarkable example of cold adaptation, but how these unique proteins arose is unknown. We have found that the antifreeze glycoproteins (AFGPs) of the predominant Antarctic fish taxon, the notothenio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen L,DeVries AL,Cheng CH

更新日期：1997-04-15 00:00:00