Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:As an important medicinal material constituting a variety of traditional Chinese medicine prescriptions, Nepeta angustifolia C. Y. Wu was used as a folk medicine to treat various vascular-related diseases including apoplexia, and cerebral haemorrhage in Tibet, China. Our previous studies have shown that this plant had a significant protective effect on vascular dysfunction of the intracerebral haemorrhage and diabetic rats. In present study, we aimed to investigate the protective effects and underlying mechanisms of Nepeta angustifolia on diabetic nephropathy (DN), a microvascular complication. AIM OF THE STUDY:This study is aim to evaluate the protective effect of ethanol extracts of N. angustifolia (NA) on DN, and explore mechanism of action to provide basis for its pharmacological action against DN. MATERIALS AND METHODS:High-fat diet and low-dose streptozotocin administration (HFD/STZ) induced diabetic rats were randomly divided into 5 groups (n = 8): the diabetic model group, metformin group, and three dose groups of NA (60 mg/kg, 120 mg/kg, 240 mg/kg). After administration of NA for 8 weeks, the blood, urine and renal tissue were collected for subsequent experiments. Biochemical markers (urine protein, Cr, BUN), oxidative stress makers (SOD, GSH-px and MDA) and pro-inflammatory mediators (TNF-α, IL-1β, IL-6 and MCP-1) were evaluated by commercial kit and ELISA, respectively. The effect of NA on DN was further confirmed by evaluation of renal histopathology by using the H&E, PAS and Masson staining. The H2O2-induced HBZY-1 cells (rat glomerular mesangial cells) were also been used to evaluate the renal protective effect of NA (50 μg/mL, 100 μg/mL, 200 μg/mL). The oxidative stress makers were detected by commercial kit. The levels of apoptosis and related proteins (caspase 3, 9) were detected by TUNEL assay and western blot analysis, respectively. The depolarization of mitochondrial membrane potential was detected by JC-1 staining assay. RESULTS:The administration of NA is helpful to maintain near normal body weight, blood glucose, urine volume, urine protein, kidney index and serum levels of Cr and BUN. NA treatment significantly improve renal dysfunction by the down-regulation of renal oxidative stress and pro-inflammatory mediators in HFD/STZ induced diabetic rats. In vitro experiments, NA has a significant cellular protective effect in H2O2-induced HBZY-1 cells, as well as the regulation in increases of SOD level and the decreases of ROS and MDA levels. Furthermore, NA treatment can significantly inhibit H2O2 induced mesangial cells apoptosis by the increasing mitochondrial potential and suppressing caspases-madiated signaling pathway. CONCLUSIONS:NA has obvious improvement on renal dysfunction in HFD/STZ induced diabetic rats. NA can protect mesangial cells by inhibiting oxidative stress induced apoptosis, which may be related to its regulation of mitochondrial-caspase apoptosis pathway.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Huang S,Tan M,Guo F,Dong L,Liu Z,Yuan R,Dongzhi Z,Lee DS,Wang Y,Li Bdoi
10.1016/j.jep.2020.112771subject
Has Abstractpub_date
2020-06-12 00:00:00pages
112771eissn
0378-8741issn
1872-7573pii
S0378-8741(19)35075-5journal_volume
255pub_type
杂志文章abstract::By means of bioassay-guided separation methods, the cancer cell growth inhibitory constituents residing in the bark, stem and leaves of the Mauritius medicinal plant Terminalia arjuna (Combretaceae) were examined. The cancer cell line active components were found to be gallic acid, ethyl gallate, and the flavone luteo...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/S0378-8741(96)01421-3
更新日期:1996-08-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Yan-Hou-Qing (YHQ), a Chinese medicine formula containing fourteen kinds of materials, has been designed for pharyngitis and cough treatment in Oriental medicine. In the present study, the anti-allergic effects and underlying mechanisms of YHQ in inhibition of airway hyper responsiveness ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.11.038
更新日期:2019-03-01 00:00:00
abstract::This study investigates anticonvulsant effects of an essential oil of the fruits of Pimpinella anisum (Umbelliferae), a folkloric remedy in the Iranian traditional medicine, against seizures induced by pentylenetetrazole (PTZ) or maximal electroshock (MES) in male mice. The essential oil suppressed tonic convulsions i...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(98)00161-5
更新日期:1999-08-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Maimendong and Qianjinweijing Tang (Jin formula), a classic Chinese formula, can enhance therapeutic efficacy and reduce adverse effects in patients with lung cancer. AIM OF THE STUDY:To evaluate the anti-lung cancer effect of Jin formula in vivo and in vitro, and to explore the role of ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112836
更新日期:2020-08-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Radix paeoniae rubra, also known as chishao (CS), is a frequently used traditional Chinese medicine that can promote blood circulation to remove blood stasis. It has been widely used for the prevention and treatment of cardiovascular diseases in China. Although terpene glycoside (TG), the...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.05.028
更新日期:2017-07-12 00:00:00
abstract::The anti-hyperglycemic action of Hei-Shug-Pian, the fire-processed product of the root of Aconitum (Aconitum carmichaeli), was investigated in streptozotocin-induced diabetic (STZ-diabetic) rats. At 120 min following oral administration of Hei-Shug-Pian at doses ranging from 12.5 to 50 mg/kg, plasma glucose of STZ-dia...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.01.001
更新日期:2006-06-30 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Propolis has been employed extensively in many cultures since ancient times as antiseptic, wound healing, anti-pyretic and others due to its biological and pharmacological properties, such as immunomodulatory, antitumor, anti-inflammatory, antioxidant, antibacterial, antiviral, antifungal...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112174
更新日期:2019-12-05 00:00:00
abstract::The aim of this study was to investigate the immunomodulatory effects of the volatile oil of ginger (Zingiber officinale Roscoe) in vitro and in vivo in mice. In vitro, the volatile oil of ginger (0.001-10 ng/mL) significantly inhibited T lymphocyte proliferation (P < 0.01), decreased the number of the total T lymphoc...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.10.022
更新日期:2006-04-21 00:00:00
abstract:AIM OF THE STUDY:In African traditional medicine, different parts of Phyllanthus amarus Schum. and Thonn. (family: Euphorbiaceae) are highly valued for the treatment of array of human diseases including hepatic and urolithic and/or other renal diseases. In the present study, single oral 100-400mg/kg/day of the leaf and...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2008.04.025
更新日期:2008-07-23 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Herbal drugs are gaining worldwide prominence due to their distinct advantages. Developing countries have started exploring the ethnopharmacological approach of drug discovery and have begun to file patents on herbal drugs. The expansion of R&D in Indian herbal research organizations and ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.05.022
更新日期:2011-09-01 00:00:00
abstract::The present study was undertaken to explore the immunomodulatory activity of ethanolic and water extracts of Capparis zeylanica Linn. (family: Capparidaceae) leaves on neutrophil adhesion test, humoral response to sheep red blood cells, delayed-type hypersensitivity, phagocytic activity and cyclophosphamide-induced my...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.03.041
更新日期:2006-11-24 00:00:00
abstract::The oral hypoglycaemic activity of Momordica charantia fruit juice was investigated in rats with streptozotocin-induced diabetes. Oral administration of the juice (10 ml/kg for 30 days) did not show a significant effect, either acute or cumulative, on the ability to tolerate an external glucose load. The glycosylated ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(90)90008-h
更新日期:1990-09-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Astragali radix is one of the well-known traditional Chinese herbal medicine, and possesses various biological functions, such as hepatoprotective and anticancer. In present study, to investigate the metabolism and pharmacokinetics of the major constituents of A. radix, a sensitive ultra-...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.04.019
更新日期:2015-07-01 00:00:00
abstract:AIMS OF THE STUDY:This study investigated the anti-inflammatory and analgesic activities, and protoberberine alkaloid contents of ethanol extract of MO roots (MOR(EtOH)). MATERIALS AND METHODS:The analgesic activity of MOR(EtOH) was determined using acetic acid-induced writhing response and formalin test. The anti-inf...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.06.024
更新日期:2009-09-07 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Astragaloside IV is the primary pure saponin isolated from Astragalus membranaceus, one of the valuable traditional medical herbs. Antifibrotic activities of Astragalus membranaceus have been extensively proved. AIM OF THE STUDY:To investigate the effects of astragaloside IV on hepatic s...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.01.035
更新日期:2009-04-21 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for scre...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.02.017
更新日期:2011-04-26 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Plants of genus Stemona (Stemonaceae) have been long used locally and traditionally in many South and East Asian counties to relieve cough, dispel phlegm, prevent asthma, control pests, diminish inflammation, decrease pain, and treat some cutaneous diseases. AIM OF STUDY:This review prov...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2020.113112
更新日期:2021-01-30 00:00:00
abstract::Four taxoids (taxusin, baccatin VI, baccatin III and 1beta-hydroxybaccatin I) and five lignans (lariciresinol, taxiresinol, 3'-demethylisolariciresinol-9'-hydroxyisopropylether, isolariciresinol and 3-demethylisolariciresinol) were isolated from the heartwood of Taxus baccata L. (Taxaceae) growing in Turkey through ch...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2003.09.005
更新日期:2003-12-01 00:00:00
abstract::Hydroethanol (HE) and methanol (ME) extracts obtained from the leaves of Passiflora actinia Hooker were evaluated for behavioral effects in mice. Single-dose oral administration of HE (300 and 600 mg/kg) or ME (100 and 300 mg/kg) resulted in anxiolytic-like effects in the elevated plus-maze. The anxiolytic-like effect...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.11.021
更新日期:2007-05-04 00:00:00
abstract::This paper focusses on Xhosa healers and their pharmacopoeias in Southern Africa. Its preliminary aim is to show that Xhosa healers have incorporated Western pharmaceutical products into their traditional dispensaries. The primary aim of the paper is to explain the trend towards medicinal incorporation in terms of the...
journal_title:Journal of ethnopharmacology
pub_type: 历史文章,杂志文章
doi:10.1016/0378-8741(91)90083-p
更新日期:1991-07-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Doradilla is a plant that has a long history in the Mexican traditional system of medicine for gall and renal stones, diuresis, stomach and liver inflammation among other diseases. Major components isolated from these plants include biflavonoids as amentoflavone (1), robustaflavone (2) an...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2015.01.031
更新日期:2015-04-02 00:00:00
abstract::All through time, humans have used smoke of medicinal plants to cure illness. To the best of our knowledge, the ethnopharmacological aspects of natural products' smoke for therapy and health care have not been studied. Mono- and multi-ingredient herbal and non-herbal remedies administered as smoke from 50 countries ac...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2006.09.005
更新日期:2006-11-24 00:00:00
abstract::This study was undertaken to investigate the protective effects of Phyllanthus emblica Linn. (PE) extract on ethanol induced rat hepatic injury. PE (0.5 and 1 mg/ml) increased cell viability of rat primary cultured hepatocytes being treated with ethanol (96 microl/m) by increasing % MTT and decreasing the release of t...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.03.035
更新日期:2006-10-11 00:00:00
abstract::A room temperature aqueous extract of the roots of Taverniera abyssinica antagonized the contractile responses of the guinea-pig ileum to acetylcholine and histamine. The extract also relaxed the smooth muscle of the rabbit duodenum, abolished the pendular contractions and antagonized the effects of acetylcholine and ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(90)90021-k
更新日期:1990-08-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Oryeongsan (ORS, Wulingsan), a formula composed of five herbal medicines, has long been used for the treatment of impairments of the regulation of body fluid homeostasis in China, Japan and Korea. AIM OF THE STUDY:The purpose of the present study was to test the effects of ORS on the ren...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2012.02.021
更新日期:2012-06-14 00:00:00
abstract:AIM OF THE STUDY:Red Ginseng roots (Panax ginseng C.A. Meyer) have traditionally been thought to have anti-allergic effects, but their influence on food-induced allergic responses is unclear. MATERIALS AND METHODS:This study examined the effects of a Red Ginseng extract on an ova-albumin (OVA)-evoked allergic reaction...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.08.012
更新日期:2010-10-28 00:00:00
abstract::The partitioned n-hexane, ethylacetate and residual methanol extracts of the root of Rumex maritimus were evaluated for antidiarrhoeal activity in mice using castor oil and serotonin induced diarrhoea and also charcoal motility test at 50, 100 and 200 mg/kg body weight dose. Among these, the methanol extract most sign...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(02)00326-4
更新日期:2003-02-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Modified Si-Miao-San (mSMS) is a prescription modified from Si-Miao-San which is an ancient Chinese prescription used to treat various ailments. AIM OF THE STUDY:Modified Si-Miao-San (mSMS) has been used for the treatment of infectious and inflammatory disorders in the clinic. This study...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.02.002
更新日期:2010-05-04 00:00:00
abstract::In the biological sciences the use of medicinal plants in indigenous cultures is commonly seen as being based on a long tradition ('traditional medicine'). However, under normal circumstances, ethnobotanical studies cannot provide evidence on the antiquity of specific uses for medicinal plants since oral traditions ha...
journal_title:Journal of ethnopharmacology
pub_type: 历史文章,杂志文章
doi:10.1016/s0378-8741(03)00188-0
更新日期:2003-10-01 00:00:00
abstract::The effect of a methanol extract of Sophora flavescens root was examined on gastric ulcers induced in rats by HCl/ethanol in order to substantiate its reputed protective properties. Oral doses of 1 g/kg completely suppressed ulceration. Results suggest that oral administration of 50 mg/kg of vexibinol, a flavanol foun...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/0378-8741(90)90053-v
更新日期:1990-05-01 00:00:00