B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches.

Abstract:

:Antibodies secreted within the intestinal tract provide protection from the invasion of microbes into the host tissues. Germinal center (GC) formation in lymph nodes and spleen strictly requires SLAM-associated protein (SAP)-mediated T cell functions; however, it is not known whether this mechanism plays a similar role in mucosal-associated lymphoid tissues. Here, we find that in Peyer's patches (PPs), SAP-mediated T cell help is required for promoting B cell selection in GCs, but not for clonal diversification. PPs of SAP-deficient mice host chronic GCs that are absent in T cell-deficient mice. GC B cells in SAP-deficient mice express AID and Bcl6 and generate plasma cells in proportion to the GC size. Single-cell IgA sequencing analysis reveals that these mice host few diversified clones that were subjected to mild selection forces. These findings demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions.

journal_name

Cell Rep

journal_title

Cell reports

authors

Biram A,Winter E,Denton AE,Zaretsky I,Dassa B,Bemark M,Linterman MA,Yaari G,Shulman Z

doi

10.1016/j.celrep.2020.01.032

subject

Has Abstract

pub_date

2020-02-11 00:00:00

pages

1910-1922.e5

issue

6

issn

2211-1247

pii

S2211-1247(20)30047-4

journal_volume

30

pub_type

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