Abstract：
:α-Actinins, a family of critical cytoskeletal actin-binding proteins that usually exist as anti-parallel dimers, play crucial roles in organizing the framework of the cytoskeleton through crosslinking the actin filaments, as well as in focal adhesion maturation. However, the molecular mechanisms underlying its functions are unclear. Here, by mechanical manipulation of single human α-actinin 1 using magnetic tweezers, we determined the mechanical stability and kinetics of the functional domains in α-actinin 1. Moreover, we identified the force-dependence of vinculin binding to α-actinin 1, with the demonstration that force is required to expose the high-affinity binding site for vinculin binding. Further, a role of the α-actinin 1 as molecular shock absorber for the cytoskeleton network is revealed. Our results provide a comprehensive analysis of the force-dependent stability and interactions of α-actinin 1, which sheds important light on the molecular mechanisms underlying its mechanotransmission and mechanosensing functions.
journal_name
Cell Repjournal_title
Cell reportsauthors
Le S,Hu X,Yao M,Chen H,Yu M,Xu X,Nakazawa N,Margadant FM,Sheetz MP,Yan Jdoi
10.1016/j.celrep.2017.11.040subject
Has Abstractpub_date
2017-12-05 00:00:00pages
2714-2723issue
10issn
2211-1247pii
S2211-1247(17)31677-7journal_volume
21pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:Epiblast cells in the early post-implantation stage mammalian embryo undergo a transition described as lineage priming before cell fate allocation, but signaling pathways acting upstream remain ill defined. Genetic studies demonstrate that Smad2/3 double-mutant mouse embryos die shortly after implantation. To learn mo...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.07.077
更新日期：2018-08-21 00:00:00
abstract：:Eosinophils mediate protection against filarial nematodes. Our results demonstrate that eosinophil extracellular traps (EETosis) are induced by microfilariae and infective L3 larvae of Litomosoides sigmodontis. These extracellular DNA traps inhibit microfilariae motility in a DNA- and contact-dependent manner in vitro...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108621
更新日期：2021-01-12 00:00:00
abstract：:Cells, from microbes to mammals, adapt their membrane lipid composition in response to environmental changes to maintain optimal properties. Global patterns of lipidome remodeling are poorly understood, particularly in organisms with simple lipid compositions that can provide insight into fundamental principles of mem...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108165
更新日期：2020-09-22 00:00:00
abstract：:Inhibition of RNA polymerase I (Pol I) is a promising strategy for modern cancer therapy. BMH-21 is a first-in-class small molecule that inhibits Pol I transcription and induces degradation of the enzyme, but how this exceptional response is enforced is not known. Here, we define key elements requisite for the respons...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.03.066
更新日期：2018-04-10 00:00:00
abstract：:Chromothripsis represents a novel phenomenon in the structural variation landscape of cancer genomes. Here, we analyze the genomes of ten patients with congenital disease who were preselected to carry complex chromosomal rearrangements with more than two breakpoints. The rearrangements displayed unanticipated complexi...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2012.05.009
更新日期：2012-06-28 00:00:00
abstract：:Stable unannotated transcripts (SUTs), some of which overlap protein-coding genes in antisense direction, are a class of non-coding RNAs. While case studies have reported important regulatory roles for several of such RNAs, their general impact on protein abundance regulation of the overlapping gene is not known. To t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.05.043
更新日期：2016-06-21 00:00:00
abstract：:Over 80% of pancreatic ductal adenocarcinoma (PDA) patients are diagnosed with non-resectable late-stage disease that lacks effective neoadjuvant therapies. Stereotactic body radiation therapy (SBRT) has shown promise as an emerging neoadjuvant approach for treating PDA, and here, we report that its combination with l...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.095
更新日期：2019-10-08 00:00:00
abstract：:Microglia are resident immune cells of the CNS that are activated by infection, neuronal injury, and inflammation. Here, we utilize flow cytometry and deep RNA sequencing of acutely isolated spinal cord microglia to define their activation in vivo. Analysis of resting microglia identified 29 genes that distinguish mic...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.06.018
更新日期：2013-07-25 00:00:00
abstract：:Neuregulin-1 (NRG1) gene variants are associated with increased genetic risk for schizophrenia. It is unclear whether risk haplotypes cause elevated or decreased expression of NRG1 in the brains of schizophrenia patients, given that both findings have been reported from autopsy studies. To study NRG1 functions in vivo...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.07.026
更新日期：2014-08-21 00:00:00
abstract：:BRCA1 is a tumor suppressor found to be mutated in hereditary breast and ovarian cancer and plays key roles in the maintenance of genomic stability by homologous recombination repair. It is recruited to damaged chromatin as a component of the BRCA1-A deubiquitinase, which cleaves K63-linked ubiquitin chains attached t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.11.063
更新日期：2016-12-20 00:00:00
abstract：:Glucagon-like peptide-1 receptor (GLP-1R) agonists used to treat type 2 diabetes mellitus often produce nausea, vomiting, and in some patients, undesired anorexia. Notably, these behavioral effects are caused by direct central GLP-1R activation. Herein, we describe the creation of a GLP-1R agonist conjugate with modif...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107768
更新日期：2020-06-16 00:00:00
abstract：:Large-scale, cooperative rearrangements underlie the functions of RNA in RNA-protein machines and gene regulation. To understand how such rearrangements are orchestrated, we used high-throughput chemical footprinting to dissect a seemingly concerted rearrangement in P5abc RNA, a paradigm of RNA folding studies. With m...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.02.101
更新日期：2018-03-20 00:00:00
abstract：:Human rhinovirus (HRV) causes upper respiratory infections and asthma exacerbations. We screened multiple orthologous RNAi reagents and identified host proteins that modulate HRV replication. Here, we show that RNASEK, a transmembrane protein, was needed for the replication of HRV, influenza A virus, and dengue virus....
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.06.076
更新日期：2015-08-04 00:00:00
abstract：:To understand how the Bcl6 transcriptional repressor functions in the immune system, we disrupted its RD2 repression domain in mice. Bcl6RD2(MUT) mice exhibit a complete loss of germinal center (GC) formation but retain normal extrafollicular responses. Bcl6RD2(MUT) antigen-engaged B cells migrate to the interfollicul...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.07.059
更新日期：2014-09-11 00:00:00
abstract：:Training-dependent increases in c-fos have been used to identify engram cells encoding long-term memories (LTMs). However, the interaction between transcription factors required for LTM, including CREB and c-Fos, and activating kinases such as phosphorylated ERK (pERK) in the establishment of memory engrams has been u...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.11.022
更新日期：2018-12-04 00:00:00
abstract：:Selecting a suitable site to deposit their eggs is an important reproductive need of Drosophila females. Although their choosiness toward egg-laying sites is well documented, the specific neural mechanism that activates females' search for attractive egg-laying sites is not known. Here, we show that distention and con...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.09.033
更新日期：2014-10-23 00:00:00
abstract：:Tissue-resident memory T (TRM) cells provide key adaptive immune responses in infection, cancer, and autoimmunity. However, transcriptional heterogeneity of human intestinal TRM cells remains undefined. Here, we investigate transcriptional and functional heterogeneity of human TRM cells through study of donor-derived ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108661
更新日期：2021-01-19 00:00:00
abstract：:Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.01.059
更新日期：2015-03-03 00:00:00
abstract：:Generation of insulin-secreting β cells in vitro is a promising approach for diabetes cell therapy. Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are differentiated to β cells (SC-β cells) and mature to undergo glucose-stimulated insulin secretion, but molecular regulation of thi...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107687
更新日期：2020-05-26 00:00:00
abstract：:Gli proteins are transcriptional effectors of the Hedgehog (Hh) pathway in both normal development and cancer. We describe a program of multisite phosphorylation that regulates the conversion of Gli proteins into transcriptional activators. In the absence of Hh ligands, Gli activity is restrained by the direct phospho...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.12.003
更新日期：2014-01-16 00:00:00
abstract：:Recurrent copy-number alterations, mutations, and transcript fusions of the genes encoding CDKs/cyclins are characterized in >10,000 tumors. Genomic alterations of CDKs/cyclins are dominantly driven by copy number aberrations. In contrast to cell-cycle-related CDKs/cyclins, which are globally amplified, transcriptiona...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107884
更新日期：2020-07-14 00:00:00
abstract：:During an infection the antigen-nonspecific memory CD8 T cell compartment is not simply an inert pool of cells, but becomes activated and cytotoxic. It is unknown how these cells contribute to the clearance of an infection. We measured the strength of T cell receptor (TCR) signals that bystander-activated, cytotoxic C...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.02.020
更新日期：2013-03-28 00:00:00
abstract：:During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.04.054
更新日期：2014-06-12 00:00:00
abstract：:Synapsin III (SynIII) is a phosphoprotein that is highly expressed at early stages of neuronal development. Whereas in vitro evidence suggests a role for SynIII in neuronal differentiation, in vivo evidence is lacking. Here, we demonstrate that in vivo downregulation of SynIII expression affects neuronal migration and...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.03.022
更新日期：2015-04-14 00:00:00
abstract：:Bat cells and tissue have elevated basal expression levels of antiviral genes commonly associated with interferon alpha (IFNα) signaling. Here, we show Interferon Regulatory Factor 1 (IRF1), 3, and 7 levels are elevated in most bat tissues and that, basally, IRFs contribute to the expression of type I IFN ligands and ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108345
更新日期：2020-11-03 00:00:00
abstract：:Neurogenesis continues in the ventricular-subventricular zone (V-SVZ) of the adult forebrain from quiescent neural stem cells (NSCs). V-SVZ NSCs are a reservoir for new olfactory bulb (OB) neurons that migrate through the rostral migratory stream (RMS). To generate neurons, V-SVZ NSCs need to activate and enter the ce...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.12.094
更新日期：2018-01-23 00:00:00
abstract：:Durable humoral immunity against epidemic infectious disease requires the survival of long-lived plasma cells (LLPCs). LLPC longevity is dependent on metabolic programs distinct from short-lived plasma cells (SLPCs); however, the mechanistic basis for this difference is unclear. We have previously shown that CD28, the...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107815
更新日期：2020-06-23 00:00:00
abstract：:In mammals, a cell's decision to divide is thought to be under the control of the Rb/E2F pathway. We previously found that inactivation of the Rb family of cell cycle inhibitors (Rb, p107, and p130) in quiescent liver progenitors leads to uncontrolled division and cancer initiation. Here, we show that, in contrast, de...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.06.025
更新日期：2014-07-24 00:00:00
abstract：:Although transcription factors are prevalent among yeast prion proteins, the role of prion-mediated transcriptional regulation remains elusive. Here, we show that the yeast prion [SWI(+)] abolishes flocculin (FLO) gene expression and results in a complete loss of multicellularity. Further investigation demonstrates th...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.11.060
更新日期：2015-12-29 00:00:00
abstract：:MicroRNAs play an important role in T cell responses. However, how microRNAs regulate CD8 T cell memory remains poorly defined. Here, we found that miR-150 negatively regulates CD8 T cell memory in vivo. Genetic deletion of miR-150 disrupted the balance between memory precursor and terminal effector CD8 T cells follow...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.08.060
更新日期：2017-09-12 00:00:00
