Abstract：
:Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped potential of "coding introns" (i.e., introns between coding exons). Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest using intronically inserted "plug-and-play" cassettes (called "Trojan exons") that carry a splice acceptor site followed by the coding sequences of T2A peptide and an effector transgene. We demonstrate the efficacy of this approach in Drosophila using lines containing suitable MiMIC (Minos-mediated integration cassette) transposons and a palette of Trojan exons capable of expressing a range of commonly used transcription factors. We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system.
journal_name
Cell Repjournal_title
Cell reportsauthors
Diao F,Ironfield H,Luan H,Diao F,Shropshire WC,Ewer J,Marr E,Potter CJ,Landgraf M,White BHdoi
10.1016/j.celrep.2015.01.059subject
Has Abstractpub_date
2015-03-03 00:00:00pages
1410-21issue
8issn
2211-1247pii
S2211-1247(15)00101-1journal_volume
10pub_type
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