Abstract：
:Plant glutamate receptor homologs (GLRs) have long been proposed to function as ligand-gated Ca2+ channels, but no in planta evidence has been provided. Here, we present genetic evidence that Arabidopsis GLR3.1 and GLR3.5 form Ca2+ channels activated by L-methionine (L-Met) at physiological concentrations and regulate stomatal apertures and plant growth. The glr3.1/3.5 mutations resulted in a lower cytosolic Ca2+ level, defective Ca2+-induced stomatal closure, and Ca2+-deficient growth disorder, all of which involved L-Met. Patch-clamp analyses of guard cells showed that GLR3.1/3.5 Ca2+ channels are activated specifically by L-Met, with the activation abolished in glr3.1/3.5. Moreover, GLR3.1/3.5 Ca2+ channels are distinct from previously characterized ROS-activated Ca2+ channels and act upstream of ROS, providing Ca2+ transients necessary for the activation of NADPH oxidases. Our data indicate that GLR3.1/3.5 constitute L-Met-activated Ca2+ channels responsible for maintaining basal [Ca2+]cyt, play a pivotal role in plant growth, and act upstream of ROS, thereby regulating stomatal aperture.
journal_name
Cell Repjournal_title
Cell reportsauthors
Kong D,Hu HC,Okuma E,Lee Y,Lee HS,Munemasa S,Cho D,Ju C,Pedoeim L,Rodriguez B,Wang J,Im W,Murata Y,Pei ZM,Kwak JMdoi
10.1016/j.celrep.2016.11.015subject
Has Abstractpub_date
2016-12-06 00:00:00pages
2553-2561issue
10issn
2211-1247pii
S2211-1247(16)31556-Xjournal_volume
17pub_type
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