Abstract:
:Chromatin states must be maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications is central in this process. However, the histone modification landscape is challenged by incorporation of new unmodified histones during each cell cycle, and the principles governing heritability remain unclear. We take a quantitative computational modeling approach to describe propagation of histone H3K27 and H3K36 methylation states. We measure combinatorial H3K27 and H3K36 methylation patterns by quantitative mass spectrometry on subsequent generations of histones. Using model comparison, we reject active global demethylation and invoke the existence of domains defined by distinct methylation endpoints. We find that H3K27me3 on pre-existing histones stimulates the rate of de novo H3K27me3 establishment, supporting a read-write mechanism in timely chromatin restoration. Finally, we provide a detailed quantitative picture of the mutual antagonism between H3K27 and H3K36 methylation and propose that it stabilizes epigenetic states across cell division.
journal_name
Cell Repjournal_title
Cell reportsauthors
Alabert C,Loos C,Voelker-Albert M,Graziano S,Forné I,Reveron-Gomez N,Schuh L,Hasenauer J,Marr C,Imhof A,Groth Adoi
10.1016/j.celrep.2019.12.060subject
Has Abstractpub_date
2020-01-28 00:00:00pages
1223-1234.e8issue
4issn
2211-1247pii
S2211-1247(19)31717-6journal_volume
30pub_type
杂志文章相关文献
Cell Reports文献大全abstract::Telomerase biogenesis is a complex process where several steps remain poorly understood. Single-strand-selective uracil-DNA glycosylase (SMUG1) associates with the DKC1-containing H/ACA ribonucleoprotein complex, which is essential for telomerase biogenesis. Herein, we show that SMUG1 interacts with the telomeric RNA ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.07.040
更新日期:2019-08-13 00:00:00
abstract::Pathogens trigger T cells to express distinct sets of effector proteins. To better understand the molecular mechanisms that drive functional specification, we used high-resolution mass spectrometry and label-free protein quantification to measure proteomic differences between the seven largest circulating human CD8+ T...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.04.014
更新日期:2017-05-02 00:00:00
abstract::The stress-activated protein kinases c-Jun N-terminal kinase (JNK) and p38 are important players in cell-fate decisions in response to environmental stress signals. Crosstalk signaling between JNK and p38 is emerging as an important regulatory mechanism in inflammatory and stress responses. However, it is unknown how ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.08.020
更新日期:2018-09-04 00:00:00
abstract::Arrival of encephalitogenic T cells at inflammatory foci represents a critical step in development of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. EBI2 and its ligand, 7α,25-OHC, direct immune cell localization in secondary lymphoid organs. CH25H and CYP7B1 hydroxylate chol...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.01.020
更新日期:2017-01-31 00:00:00
abstract::We assessed the subsistence-related variation of the human gut microbiome at a fine resolution for two of the main dimensions of microbiome variation, age and geography. For this, we investigated the fecal microbiome and metabolome in rural Bassa and urbanized individuals from Nigeria, including infants, and compared ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.05.018
更新日期:2018-06-05 00:00:00
abstract::The large number of mutations identified across all cancers represents an untapped reservoir of targets that can be useful for therapeutic targeting if highly selective, mutation-specific reagents are available. We report here our attempt to generate such reagents: monoclonal antibodies against the most common R175H, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.112
更新日期:2018-01-02 00:00:00
abstract::Mucus separates gut-luminal microbes from the tissue. It is unclear how pathogens like Salmonella Typhimurium (S.Tm) can overcome this obstacle. Using live microscopy, we monitored S.Tm interactions with native murine gut explants and studied how mucus affects the infection. A dense inner mucus layer covers the distal...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.04.106
更新日期:2019-05-28 00:00:00
abstract::In response to DNA double-strand breaks (DSBs), H2AX is rapidly phosphorylated at Ser139 to promote DSB repair. Here we show that H2AX is rapidly stabilized in response to DSBs to efficiently generate γH2AX foci. This mechanism operated even in quiescent cells that barely expressed H2AX. H2AX stabilization resulted fr...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.11.054
更新日期:2015-12-29 00:00:00
abstract::This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that the number of unique epitopes targeted ("antibody breadth") increases...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.03.097
更新日期:2019-04-30 00:00:00
abstract::Granule cells (GCs) of the cerebellar input layer express high-affinity δ GABAA subunit-containing GABAA receptors (δGABAARs) that respond to ambient GABA levels and context-dependent neuromodulators like steroids. We find that GC-specific deletion of δGABAA (cerebellar [cb] δ knockout [KO]) decreases tonic inhibition...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108338
更新日期:2020-11-03 00:00:00
abstract::Activation of postsynaptic metabotropic glutamate receptors (mGluRs) modulates neuronal excitability and synaptic plasticity, while deregulation of mGluR signaling has been implicated in neurodevelopmental disorders. Overstimulation of mGluRs is restricted by the rapid endocytosis of receptors after activation. Howeve...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.08.102
更新日期:2019-10-08 00:00:00
abstract::The JAK/STAT1 pathway is generally activated by cytokines, providing essential antiviral defense. Here, we identify that STAT1 activation is independent of cytokines and JAKs at the early infection stage of some viruses, including influenza A virus (IAV). Instead, STAT1 is activated mainly through spleen tyrosine kina...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108627
更新日期:2021-01-19 00:00:00
abstract::Infections can result in a temporarily restricted unresponsiveness of the innate immune response, thereby limiting pathogen control. Mechanisms of such unresponsiveness are well studied in lipopolysaccharide tolerance; however, whether mechanisms of tolerance limit innate immunity during virus infection remains unknow...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.02.101
更新日期:2020-03-17 00:00:00
abstract::The number of essential telomerase components in the active ribonucleoprotein (RNP) has important implications for its mechanism of action yet is by and large unknown. We report that two differentially tagged TLC1 RNAs endogenously expressed in a heterozygous diploid and simultaneously detected via multi-color fluores...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.06.045
更新日期:2015-07-21 00:00:00
abstract::Alcohol produces both stimulant and sedative effects in humans and rodents. In humans, alcohol abuse disorder is associated with a higher stimulant and lower sedative responses to alcohol. Here, we show that this association is conserved in mice and demonstrate a causal link with another liability factor: low expressi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.09.059
更新日期:2019-10-29 00:00:00
abstract::The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.12.024
更新日期:2016-01-12 00:00:00
abstract::Calcium is a key regulator of mitochondrial function under both normal and pathological conditions. The mechanisms linking metabolic activity to mitochondrial Ca2+ signaling remain elusive, however. Here, by monitoring mitochondrial Ca2+ transients while manipulating mitochondrial membrane potential (ΔΨm), we found th...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.11.084
更新日期:2018-12-18 00:00:00
abstract::Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT)...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.05.033
更新日期:2015-06-30 00:00:00
abstract::Cognitive dysfunction and decreased mobility from aging and neurodegenerative conditions, such as Parkinson and Alzheimer diseases, are major biomedical challenges in need of more effective therapies. Increasing brain resilience may represent a new treatment strategy. Klotho, a longevity factor, enhances cognition whe...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.07.024
更新日期:2017-08-08 00:00:00
abstract::Current treatment regimens for pancreatic ductal adenocarcinoma (PDAC) yield poor 5-year survival, emphasizing the critical need to identify druggable targets essential for PDAC maintenance. We developed an unbiased and in vivo target discovery approach to identify molecular vulnerabilities in low-passage and patient-...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.05.063
更新日期:2016-06-28 00:00:00
abstract::The pharyngeal arch arteries (PAAs) are transient embryonic blood vessels that mature into critical segments of the aortic arch and its branches. Although defects in PAA development cause life-threating congenital cardiovascular defects, the molecular mechanisms that orchestrate PAA morphogenesis remain unclear. Throu...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.07.002
更新日期:2017-07-25 00:00:00
abstract::In Drosophila, it is thought that peptidoglycan recognition proteins (PGRPs) SA and LC structurally discriminate between bacterial peptidoglycans with lysine (Lys) or diaminopimelic (DAP) acid, respectively, thus inducing differential antimicrobial transcription response. Here, we find that accessibility to PG at the ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.04.103
更新日期:2019-05-21 00:00:00
abstract::Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia b...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.12.065
更新日期:2017-01-17 00:00:00
abstract::53BP1 plays multiple roles in mammalian DNA damage repair, mediating pathway choice and facilitating DNA double-strand break repair in heterochromatin. Although it possesses a C-terminal BRCT2 domain, commonly involved in phospho-peptide binding in other proteins, initial recruitment of 53BP1 to sites of DNA damage de...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.10.074
更新日期:2015-12-15 00:00:00
abstract::The circadian clock generates biological rhythms of metabolic and physiological processes, including the sleep-wake cycle. We previously identified a missense mutation in the flavin adenine dinucleotide (FAD) binding pocket of CRYPTOCHROME2 (CRY2), a clock protein that causes human advanced sleep phase. This prompted ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.03.041
更新日期:2017-04-11 00:00:00
abstract::Mcm2-7 helicase is loaded onto double-stranded origin DNA as an inactive double hexamer (DH) in G1 phase. The mechanisms of Mcm2-7 remodeling that trigger helicase activation in S phase remain unknown. Here, we develop an approach to detect and purify the endogenous DHs directly. Through cellular fractionation, we pro...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.11.018
更新日期:2015-12-22 00:00:00
abstract::The transcription factor hepatic leukemia factor (HLF) is strongly expressed in hematopoietic stem cells (HSCs) and is thought to influence both HSC self-renewal and leukemogenesis. However, the physiological role of HLF in hematopoiesis and HSC function is unclear. Here, we report that mice lacking Hlf are viable wit...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.084
更新日期:2017-12-19 00:00:00
abstract::We report the emergence of an endogenous circadian clock that regulates organogenesis in mouse fetal kidney. We detect circadian rhythms both in vivo with transcriptional profiling and ex vivo by bioluminescence. High-resolution structural analysis of embryonic explants reveals that global or local clock disruption re...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107661
更新日期:2020-05-19 00:00:00
abstract::Synaptic efficacy plays crucial roles in neuronal circuit operation and synaptic plasticity. Presynaptic determinants of synaptic efficacy are neurotransmitter content in synaptic vesicles and the number of vesicles undergoing exocytosis at a time. Bursts of presynaptic firings depress synaptic efficacy, mainly due to...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.02.080
更新日期:2018-03-20 00:00:00
abstract::Plant glutamate receptor homologs (GLRs) have long been proposed to function as ligand-gated Ca2+ channels, but no in planta evidence has been provided. Here, we present genetic evidence that Arabidopsis GLR3.1 and GLR3.5 form Ca2+ channels activated by L-methionine (L-Met) at physiological concentrations and regulate...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.11.015
更新日期:2016-12-06 00:00:00