Abstract：
:In Drosophila, it is thought that peptidoglycan recognition proteins (PGRPs) SA and LC structurally discriminate between bacterial peptidoglycans with lysine (Lys) or diaminopimelic (DAP) acid, respectively, thus inducing differential antimicrobial transcription response. Here, we find that accessibility to PG at the cell wall plays a central role in immunity to infection. When wall teichoic acids (WTAs) are genetically removed from S. aureus (Lys type) and Bacillus subtilis (DAP type), thus increasing accessibility, the binding of both PGRPs to either bacterium is increased. PGRP-SA and -LC double mutant flies are more susceptible to infection with both WTA-less bacteria. In addition, WTA-less bacteria grow better in PGRP-SA/-LC double mutant flies. Finally, infection with WTA-less bacteria abolishes any differential activation of downstream antimicrobial transcription. Our results indicate that accessibility to cell wall PG is a major factor in PGRP-mediated immunity and may be the cause for discrimination between classes of pathogens.
journal_name
Cell Repjournal_title
Cell reportsauthors
Vaz F,Kounatidis I,Covas G,Parton RM,Harkiolaki M,Davis I,Filipe SR,Ligoxygakis Pdoi
10.1016/j.celrep.2019.04.103subject
Has Abstractpub_date
2019-05-21 00:00:00pages
2480-2492.e6issue
8issn
2211-1247pii
S2211-1247(19)30583-2journal_volume
27pub_type
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