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Synthetic chimeric nucleases function for efficient genome editing.

Abstract：

:CRISPR-Cas systems have revolutionized genome editing across a broad range of biotechnological endeavors. Many CRISPR-Cas nucleases have been identified and engineered for improved capabilities. Given the modular structure of such enzymes, we hypothesized that engineering chimeric sequences would generate non-natural variants that span the kinetic parameter landscape, and thus provide for the rapid selection of nucleases fit for a particular editing system. Here, we design a chimeric Cas12a-type library with approximately 560 synthetic chimeras, and select several functional variants. We demonstrate that certain nuclease domains can be recombined across distantly related nuclease templates to produce variants that function in bacteria, yeast, and human cell lines. We further characterize selected chimeric nucleases and find that they have different protospacer adjacent motif (PAM) preferences and the M44 chimera has higher specificity relative to wild-type (WT) sequences. This demonstration opens up the possibility of generating nuclease sequences with implications across biotechnology.

journal_name

Nat Commun

journal_title

Nature communications

authors

Liu RM,Liang LL,Freed E,Chang H,Oh E,Liu ZY,Garst A,Eckert CA,Gill RT

doi

10.1038/s41467-019-13500-y

subject

Has Abstract

pub_date

2019-12-04 00:00:00

pages

5524

issue

1

issn

2041-1723

pii

10.1038/s41467-019-13500-y

journal_volume

10

pub_type

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