Abstract:
:Adeno-associated virus (AAV) capsid engineering is an emerging approach to advance gene therapy. However, a systematic analysis on how each capsid amino acid contributes to multiple functions remains challenging. Here we show proof-of-principle and successful application of a novel approach, termed AAV Barcode-Seq, that allows us to characterize phenotypes of hundreds of different AAV strains in a high-throughput manner and therefore overcomes technical difficulties in the systematic analysis. In this approach, we generate DNA barcode-tagged AAV libraries and determine a spectrum of phenotypes of each AAV strain by Illumina barcode sequencing. By applying this method to AAV capsid mutant libraries tagged with DNA barcodes, we can draw a high-resolution map of AAV capsid amino acids important for the structural integrity and functions including receptor binding, tropism, neutralization and blood clearance. Thus, Barcode-Seq provides a new tool to generate a valuable resource for virus and gene therapy research.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Adachi K,Enoki T,Kawano Y,Veraz M,Nakai Hdoi
10.1038/ncomms4075subject
Has Abstractpub_date
2014-01-01 00:00:00pages
3075issn
2041-1723pii
ncomms4075journal_volume
5pub_type
杂志文章abstract::Homozygosity mapping is a powerful method for identifying mutations in patients with recessive conditions, especially in consanguineous families or isolated populations. Historically, it has been used in conjunction with genotypes from highly polymorphic markers, such as DNA microsatellites or common SNPs. Traditional...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20584-4
更新日期:2021-01-22 00:00:00
abstract::To reconstruct the evolutionary origin of multicellular animals from their unicellular ancestors, the genome sequences of diverse unicellular relatives are essential. However, only the genome of the choanoflagellate Monosiga brevicollis has been reported to date. Here we completely sequence the genome of the filastere...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3325
更新日期:2013-01-01 00:00:00
abstract::Artificial nanoparticles accumulate a protein corona layer in biological fluids, which significantly influences their bioactivity. As nanosized obligate intracellular parasites, viruses share many biophysical properties with artificial nanoparticles in extracellular environments and here we show that respiratory syncy...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10192-2
更新日期:2019-05-27 00:00:00
abstract::A fundamental puzzle of human evolution is how we evolved to cooperate with genetically unrelated strangers in transient interactions. Group-level selection on culturally differentiated populations is one proposed explanation. We evaluate a central untested prediction of Cultural Group Selection theory, by assessing w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14416-8
更新日期:2020-02-04 00:00:00
abstract::Mycobacterium tuberculosis (MTB) infects 30% of all humans and kills someone every 20-30 s. Here we report genome-wide binding for ~80% of all predicted MTB transcription factors (TFs), and assayed global expression following induction of each TF. The MTB DNA-binding network consists of ~16,000 binding events from 154...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6829
更新日期:2015-01-12 00:00:00
abstract::To know the properties of a particle or a wave, one should measure how its energy changes with its momentum. The relation between them is called the dispersion relation, which encodes essential information of the kinetics. In a magnet, the wave motion of atomic spins serves as an elementary excitation, called a spin w...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15859
更新日期:2017-06-12 00:00:00
abstract::The primary motor cortex has an important role in the precise execution of learned motor responses. During motor learning, synaptic efficacy between sensory and primary motor cortical neurons is enhanced, possibly involving long-term potentiation and N-methyl-D-aspartate (NMDA)-specific glutamate receptor function. To...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3258
更新日期:2013-01-01 00:00:00
abstract::In the original version of this Article, the four structural depictions of orbital distributions were inadvertently omitted from Figure 5. This has now been corrected in both the HTML and PDF versions of the Article. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-019-10880-z
更新日期:2019-06-21 00:00:00
abstract::Diverse entry inhibitors targeting the gp120 subunit of the HIV-1 envelope (Env) trimer have been developed including BMS-626529, also called temsavir, a prodrug version of which is currently in phase III clinical trials. Here we report the characterization of a panel of small-molecule inhibitors including BMS-818251,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07851-1
更新日期:2019-01-03 00:00:00
abstract::Despite the maximized metal dispersion offered by single-atom catalysts, further improvement of intrinsic activity can be hindered by the lack of neighboring metal atoms in these systems. Here we report the use of isolated Pt1 atoms on ceria as "seeds" to develop a Pt-O-Pt ensemble, which is well-represented by a Pt8O...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11856-9
更新日期:2019-08-23 00:00:00
abstract::Leptin stimulates the sympathetic nervous system (SNS), energy expenditure, and weight loss; however, the underlying molecular mechanism remains elusive. Here, we uncover Sh2b1 in leptin receptor (LepR) neurons as a critical component of a SNS/brown adipose tissue (BAT)/thermogenesis axis. LepR neuron-specific deletio...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15328-3
更新日期:2020-03-23 00:00:00
abstract::Many voltage-gated potassium channels open in response to membrane depolarization and then inactivate within milliseconds. Neurons use these channels to tune their excitability. In Shaker K(+) channels, inactivation is caused by the cytoplasmic amino terminus, termed the inactivation gate. Despite having four such gat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5050
更新日期:2014-06-09 00:00:00
abstract::The most frequent genetic alterations across multiple human cancers are mutations in TP53 and the activation of the PI3K/AKT pathway, two events crucial for cancer progression. Mutations in TP53 lead to the inhibition of the tumour and metastasis suppressor TAp63, a p53 family member. By performing a mouse-human cross...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18973-w
更新日期:2020-10-14 00:00:00
abstract::Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2089
更新日期:2012-01-01 00:00:00
abstract::Carbon and hydrogen are fundamental components of organic molecules and a fascinating plethora of functions can be generated using these two elements. Yet, realizing attractive electronic structures only by using carbon and hydrogen remains challenging. Herein, we report the synthesis and properties of the C70 fragmen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17684-6
更新日期:2020-08-03 00:00:00
abstract::Changing climate will impact species' ranges only when environmental variability directly impacts the demography of local populations. However, measurement of demographic responses to climate change has largely been limited to single species and locations. Here we show that amphibian communities are responsive to clim...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06157-6
更新日期:2018-09-25 00:00:00
abstract::Amazon forests have experienced frequent and severe droughts in the past two decades. However, little is known about the large-scale legacy of droughts on carbon stocks and dynamics of forests. Using systematic sampling of forest structure measured by LiDAR waveforms from 2003 to 2008, here we show a significant loss ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05668-6
更新日期:2018-08-09 00:00:00
abstract::Microbial production of isoprene from renewable feedstock is a promising alternative to traditional petroleum-based processes. Currently, efforts to improve isoprenoid production in Saccharomyces cerevisiae mainly focus on cytoplasmic engineering, whereas comprehensive engineering of multiple subcellular compartments ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12851
更新日期:2016-09-21 00:00:00
abstract::A key element enabling the microelectronic technology advances of the past decades has been the conceptualization of complex circuits with versatile functionalities as being composed of the proper combination of basic 'lumped' circuit elements (for example, inductors and capacitors). In contrast, modern nanophotonic s...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4896
更新日期:2014-05-29 00:00:00
abstract::The original version of the Supplementary Information associated with this Article contained errors in Supplementary Figures 2, 12, 20 and 22. The HTML has been updated to include a corrected version of the Supplementary Information; the original incorrect versions of these Figures can be found as Supplementary Inform...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-019-09702-z
更新日期:2019-05-13 00:00:00
abstract::Synthetic biology tools, such as modular parts and combinatorial DNA assembly, are routinely used to optimise the productivity of heterologous metabolic pathways for biosynthesis or substrate utilisation, yet it is well established that host strain background is just as important for determining productivity. Here we ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03143-w
更新日期:2018-05-22 00:00:00
abstract::Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorog...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10855-0
更新日期:2019-06-27 00:00:00
abstract::Hybrid halide perovskite solar cells were first demonstrated in 2009 with cell efficiency quickly soaring from below 10% to more than 23% in a few years. Halide perovskites have the desirable processing simplicity but are very fragile when exposed to water and heat. This fragility represents a great challenge for the ...
journal_title:Nature communications
pub_type: 杂志文章,评审
doi:10.1038/s41467-018-07382-9
更新日期:2018-11-28 00:00:00
abstract::Rapid growth in size and complexity of biological data sets has led to the 'Big Data to Knowledge' challenge. We develop advanced data integration methods for multi-level analysis of genomic, transcriptomic, ribosomal profiling, proteomic and fluxomic data. First, we show that pairwise integration of primary omics dat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13091
更新日期:2016-10-26 00:00:00
abstract::Cell fate determination requires the cooperation between extrinsic signals and intrinsic molecules including transcription factors as well as epigenetic regulators. Nevertheless, how neural fate commitment is regulated by epigenetic modifications remains largely unclear. Here we show that transient histone deacetylati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7830
更新日期:2015-04-23 00:00:00
abstract::Artificial photosynthesis is a vibrant field of research aiming at converting abundant, low energy molecules such as water, nitrogen or carbon dioxide into fuels or useful chemicals by means of solar energy input. Photo-electrochemical reduction of carbon dioxide is an appealing strategy, aiming at reducing the greenh...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17125-4
更新日期:2020-07-13 00:00:00
abstract::Dynamic processes have many implications in functional molecules, including catalysts, enzymes, host-guest complexes, and molecular machines. Here, we demonstrate via deuterium NMR relaxation experiments how halogen bonding directly impacts the dynamics in solid 2,3,5,6-tetramethylpyrazine cocrystals, catalyzing the m...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08878-8
更新日期:2019-02-22 00:00:00
abstract::Accurate prediction of an individual's phenotype from their DNA sequence is one of the great promises of genomics and precision medicine. We extend a powerful individual-level data Bayesian multiple regression model (BayesR) to one that utilises summary statistics from genome-wide association studies (GWAS), SBayesR. ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12653-0
更新日期:2019-11-08 00:00:00
abstract::The proton-activated sodium channel ASIC1 belongs to the ENaC/Degenerins family of ion channels. Little is known about gating of the pore in any member of this class. Here we outline the shape of the ion pathway of ASIC1 in the open and closed conformations by measuring apparent rates of cysteine modification by thiol...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1409
更新日期:2011-07-19 00:00:00
abstract::Despite the clinical success of Androgen Receptor (AR)-targeted therapies, reactivation of AR signalling remains the main driver of castration-resistant prostate cancer (CRPC) progression. In this study, we perform a comprehensive unbiased characterisation of LNCaP cells chronically exposed to multiple AR inhibitors (...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16126-7
更新日期:2020-05-19 00:00:00