Abstract:
:Clustered regularly interspaced short palindromic repeats, or CRISPR, has been widely accepted as a versatile genome editing tool with significant potential for medical application. Reliable allele specificity is one of the most critical elements for successful application of this technology to develop high-precision therapeutics and diagnostics. CRISPR-based genome editing tools achieve high-fidelity distinction of single-base differences in target genomic loci by structural identification of CRISPR-associated (Cas) proteins and sequences of the guide RNAs. In this review, we describe the structural features of ribonucleoprotein complex formation by CRISPR proteins and guide RNAs that eventually recognize target DNA sequences. This structural understanding provides the basis for the recent applications of enhanced single-base precision genome editing technologies for effective distinction of specific alleles.
journal_name
Trends Mol Medjournal_title
Trends in molecular medicineauthors
Lee SH,Park YH,Jin YB,Kim SU,Hur JKdoi
10.1016/j.molmed.2019.09.008subject
Has Abstractpub_date
2020-03-01 00:00:00pages
337-350issue
3eissn
1471-4914issn
1471-499Xpii
S1471-4914(19)30262-Xjournal_volume
26pub_type
杂志文章,评审abstract::Genetic causes are thought to underlie about half of infertility cases, but understanding the genetic bases has been a major challenge. Modern genomics tools allow more sophisticated exploration of genetic causes of infertility through population, family-based, and individual studies. Nevertheless, potential therapies...
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journal_title:Trends in molecular medicine
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journal_title:Trends in molecular medicine
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journal_title:Trends in molecular medicine
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