Abstract：
:The higher-order architecture observed in biological systems, like viruses, is very effective in nucleic acid transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (<30 min), effective siRNA release, and gene silencing. The complex provides capsid-like protection for siRNA against nucleases without being immunostimulatory, or cytotoxic. Our data suggests that delivery vehicles based on synthetic quaternary structures that exhibit higher-order architecture may be effective in improving delivery and release of nucleic acid cargo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Gamboa A,Urfano SF,Hernandez K,Fraser DA,Ayalew L,Slowinska Kdoi
10.1038/s41598-019-53462-1subject
Has Abstractpub_date
2019-11-14 00:00:00pages
16875issue
1issn
2045-2322pii
10.1038/s41598-019-53462-1journal_volume
9pub_type
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